CA2174244A1 - Biochemically active agents for chemical catalysis and cell receptor activation - Google Patents

Abstract

A biologically active composition made up of core particles or surfaces which are coated with a layer which is designed to allow attachment of biochemically reactive pairs (BRP's) without denaturing the BRP. BRPs which may be attached include ligand-receptor pairs, enzyme-substrate pairs, drug-receptor pairs, catalyst-reactant pairs, toxin-ligand pairs, absorbant-absorbate pairs and adsorbant-adsorbate pairs. Also disclosed are biologically active compositions made up of biodegradable core particles which are coated with a layer that is designed to allow attachment of biologically active agents without denaturing them. The compositions may further include an exterior targeting membrane which provides selective targeting to specific receptors. In addition, biologically active compositions for use in gene therapy and other transfection procedures are disclosed. The compositions are composed of nanocrystalline core particles which are coated with a layer that is designed to allow attachment of transfection agents (DNA/RNA segments or antisense fragments) without denaturing them. The composition may further include an exterior targeting membrane which provides selective targeting of the transfection agents to specific cell receptors.

Description

WO95112392 2~ 4 ~ 4 ~ PCT/US94/12515 BIOCHEMICALLY ACTIVE AGENTS FOR CHEMICAL
CATALYSIS AND CELL RECEPTOR ACTIVATION

BACKGROUND OF THE INVENTION
This is a continuation-in-part of co-pending application Serial No.
08/000,199 which was filed on January 4, 1993 which is a continuation-in-part of Serial No. 07/690,601 which was filed on April 24, 1991 which is a continuation-in-part of co-pending application Serial No. 07/542,255 which was filed on June 22, 1990.

1. Field of the Invention The present invention relates generally to synthetic biologically active compositions which have a microparticulate (nanoparticulate) core.
In one aspect, the present invention relates to biologically active compo-sitions where transfecting DNA or RNA is attached to a microparticulate core and coated with a targeting membrane or ligand. These transfection nanoparticles are useful in delivering the transfecting DNA or RNA to target cells. In another aspect, the present invention relates to synthetic biochemically active agents which are useful for chemical catalyst and/or cell receptor activation. The present invention also relates to biologically active compositions where the microparticulate core is biodegradable.

2. Descriotion of Related Art The attachment of biologically active proteins, peptides or pharma-cologic agents to various carrier particles has been an area of intense investigation. These conjugated biological systems offer the promise of reduced toxicity, increased efficacy and lowered cost of biologically active agents. As a result, many different carrier models are presently available.

WO 95/12392 2 ~ 7 ~ 2 ~ 1 PCT/US94/12515 0 (Varga, J.M., Asato, N., in Goldberg, E.P. (ed.): Polymers in Biologv and Mediçine. New York, Wiley, 2, 73-88 (1983). Ranney, D.F., Huffaker, H.H., in Juliano, R.L. (ed.): Biological ApDroaches to the Delivery of Drugs, Ann. N Y. Acad. Sci., 507, 104-119 (1987).) Nanocrystallineand micron sized inorganic substrates are the most common carriers and proteins are the most commonly conjugated agents. For example, gold/protein (prin-cipally immunoglobulin) conjugates measuring as small as 5 nm have been used in immunological labeling applications in light, transmission electron and scanning electron microscopy as well as immunoblotting. (Faulk, W., Taylor, G ., Immunochemistry 8, 1081 - 1083 (1971) . Hainfeld, J . F., Nature 333, 281-282 (1988).) Silanized iron oxide protein conjugates (again principally antibodies) generally measuring between 500 and 1500 nm have proven useful in various in vitro applications where paramagnetic properties can be used advantageously. (Research Products Catalog, Advanced Magnetics, Inc., Cambridge, MA,1988-1989.) Ugelstad and others have produced gamma iron oxides cores coated with a thin polystyrene shell. (Nustad, K., Johansen, L., Schmid, R., Ugelstad, J., Ellengsen, T., Berge, A.: Covalent coupling of proteins to monodisperse particles. Preparation of solid phase second antibody. Agents Actions 1982; 9:207-212 (id. no. 60).) The resulting 4500 nm beads demonstrated both the adsorption capabilities of polystyrene latex beads as well as the relatively novel benefit of paramag-netism.
Carrier systems designed for in vivo applications have been fabri-cated from both inorganic and organic cores. For example, Davis and Illum developed a 60 nm system comprised of polystyrene cores with the block copolymer poloxamer, polyoxyethylene and polyoxypropylene, outer coats that showed a remarkable ability to bypass rat liver and splenic macro-phages. (Davis, S.S., Illum, L., Biomaterials 9, 111-115 (1988)). Drug delivery with these particles has not yet been demonstrated. Ranney and ~ WO 95/12392 PCT/US94/12515 21 71~

Huffaker described an iron-oxide/albumin/drug system that yielded 350-1600 nm paramagnetic drug carriers. (Ranney, D.F., Huffaker, H.H., In, Juliano, R.L. (ed.): Biological approaches to the deliverv of drugs, Ann.
N.Y. Acad. Sci. 507, 104-119 (1987).) Poznasky has developed an enzyme-albumin conjugate system that appears to decrease the sensitivity of the product to biodegradation while masking the apparent antigenicity of the native enzyme. (Poznasky, M.J.: Targeting enzyme albumin conju-gates. Examining the magic bullet. In, Juliano, R.L. (ed.): Biological a~proaches to the delivery of drugs, Annals New York Academy Sciences 1987; 507-211 :219.) Shaw and others have prepared and characterized lipoprotein/drug complexes. (Shaw, J.M., Shaw, K.V., Yanovich, S., Iwanik, M., Futch, W.S., Rosowsky, A., Schook, L.B.: Delivery of lipophilic drugs using lipoproteins. In, Juliano, R.L.(ed.): Biological approaches to the delivery of druas,AnnalsNewYorkAcademySciences1987;507:252-271.) Lipophi-lic drugs are relatively stable in these carriers and cell interactions do occuralthough little detail is known.
In any conjugated biological composition, it is important that the conformational integrity and biological activity of the adsorbed proteins or other biological agents be preserved without evoking an untoward immuno-logical response. Spacial orientation and structural configuration are known to play a role in determining the biological activity of many peptides, proteins and pharmacological agents. Changes in the structural configuration of these compounds may result in partial or total loss of biological activity. Changes in configuration may be caused by changing the environment surrounding the biologically active compound or agent.
For example, pharmacologic agents which exhibit in vitro activity may not exhibit in vivo activity owing to the loss of the molecular configuration formerly determined in part by the in vitro environment. Further, the size and associated ability of the carrier particle to minimize phagocytic trapping 2 ~ 4_ is a primary concern when the composition is to be used in vivo. All of these factors must be taken into account when preparing a carrier particle.
Biochemical phenomena consist of binary interactions between pairs of molecules. Common names for such biochemically reactive pairs ("BRP's") include but are not limited to immunological pairs, ligand-receptor pairs, enzyme-substrate pairs, drug-receptor pairs, catalyst-reactant pairs, catalyst-substrate pairs, absorbate-absorbent pairs, adsorbate-adsorbent pairs, and toxin-ligant pairs. On a molecular level, nearly all biochemical phenomena between such pairs involve the spatial recognition of one mole-cule by another, and such recognition serves as the means by which energy and information are transmitted, products are generated, responses are initiated and complex biological structures are built.
The process of spatial recognition implies of both regioselective and stereoselective interactions among BRP's. One member of a BRP, con-strained by fundamental biophysical laws, may interact with the other member of a BRP if and only if both members are physically conformed within some bounded set of possible spatial arrangements and if both members have their respective interactive regions unencumbered. The environment within which BRP's interact affect greatly the process of spatial recognition. Environments that constain spatial mobility or encumber molecular regions may, depending on the degree of constraint and the resulting spatial conformation, either promote or inhibit BRP
interactions.
An example of the former is surface activation of synthetic chemical reactions in a process known as "solid phase synthesis." Solid phases, either as solid glassy polymers, crystalline materials, or complex macro-molecular polymers have been features of synthetic biochemistry since the early 1 960's. Their use was advanced largely by Merrifield for facilitating peptide synthesis and for which he received the Nobel Prize for Chemistry in 1984. They became widely popular because the solid-phase method ~ WO 95112392 PCT/US94/12515 ~1 742~

offered simplicity, speed, avoidance of intermediate isolation, and automation. The principal limitation in the widespread use of solid phases has been the empirical observation that only a few surfaces have been effective BRP interaction promoters.
Drug delivery systems which are used for in vivo delivery of biolo-gically active agents, tend to be very complex. Most have some advan-tages that give them promise and possibility for further development. Most are also riddled with problems ranging from low drug load to in vivo instability. Drug delivery systems which are able to deliver a moderate dose of therapeutic agent to a specific target cell are highly desirable. This type of delivery system should provide a binding of the carrier to the cell via a receptor and a mechanism of internalization where the drug is libe-rated to the intracellular space from the carrier. The carrier should also be biodegradable and not induce any type of immune reaction or response.
The delivery system should be specific, with high efficacy and low non-targeted toxicity. It should be convenient to administer, manufacture, and be economically viable for both the patient and the manufacturer.
Although numerous different carrier particlQs have been developed, there is a continuing need to provide carrier par;.~.ies for both in vivo and in vitro application wherein a biologically active peptide, protein or pharmacological agent can be attached to the particles in a manner which promotes stabilization of the biologically active compound in its active configuration. With respect to chemical catalysts and cell receptor activation, it wouid be desirable to develop synthetic surfaces to which individual catalysts may be anchored without destroying their catalytic activity. Such surfaces should also be useful for immobilizing bio-chemically reactive pairs (BRP's), such as catalyst subsl~le pairs, without reducing the ability of the BRP's to promote biochemical reactions.
To date, gene therapy in humans has been limited to ex-vivo proto-cols in which tissues are transfected in the culture dish and placed back in WO 95112392 PCT/US9~/12515 ~
2~

the body. In vivo work is still in pre-clinical development and has been confined to animal models due to a range of safety and efficacy issues.
Such concerns arise primarily from the use of viral vectors to effect the gene transfer. Retroviral transfection has generated a lot of interest since it can stably transfect nearly 100% of targeted cells ex-vivo. Production of transfecting, replication defective retroviruses, proceeds through packaging cell lines which in principle are unable to produce wild type virus. However, low titers of "wild type" (replication competent) virus have been observed in these systems. In one such protocol utilizing primates, outbreaks of Iymphoma were linked to the detection of wild type retrovirus from a packaging system. Besides potential pathogenicity, maintaining useful transfecting titers of these vectors can be difficult.
They are hard to purify and concentrate since the envelopes (membranes) tend to be extremely labile. Alternatively, adenoviral vectors have been found to be considerably more stable. Moreover, these viruses are capable of transfecting quiescent tissue and producing large amounts of gene products. Unfortunately, gene expression is often transient because the viral genome often remains extra-chromosomal. Direct clinical application is also problematic, since replication of the vector can result in aberrant host protein synthesis leading to deleterious effects ranging from oncoge-nesis to cellular toxicity.
Given the practical concerns of in vivo viral transfection, nonviral methods are also being developed. At present, most efforts are centered on receptor mediated transfer because such methods can provide targeted delivery of DNA (and RNA) in vivo. Receptor-mediated systems employ ligand DNA (and RNA) complexes which can be recognized by cell recep-tors on the cell surface. Internalization of the complex occurs via the formation of endocytotic vesicles which allow for transport into the cytoplasm. Problems arise, however, when the endosomes fuse with Iyso-somes which causes the contents to be destroyed. In turn, the trans-2~ 7~2~

fection rate for these complexes remain below clinical efficacy. Some investigators have used fusogenic peptides of Influenza Hemmoglutin A to disrupt endosome formation which has led to higher transfection rates.
Nonetheless, the data on in vivo expression suggests that this method may only permit transient expression of genes.
Besides ligand DNA (and RNA) complexes, lipofection techniques have also been tried with varying success. Liposomes are specially susceptible to uptake by the filter organs and in the peripheral tissues by macrophages which limits their transfection efficiency and specificity in 1 0 vivo.
In view of the above, it would be desirable to provide compositions which can be used to transfect cells with DNA or RNA in both in vivo or in vitro environments.

SUMMARY OF THE INVENTION
In accordance with the present invention, members of a BRP such as biologically active peptides, proteins or pharmacological agents are attached to a core particle to provide a wide variety of biologically active compositions. The invention is based on the discovery that the surface of ultrafine particles (nanocrystalline particles) can be modified with a surface coating to allow attachment of biologically active moieties to produce compositions wherein the naturally occurring structural environment of the moiety is mimicked sufficiently so that biological activity is preserved. The coating which provides for the attachment of biologically active moieties to nanocrystalline particles in accordance with the present invention can be composed of a basic or modified sugar or oligonucleo~;~le. Coating nanocrystalline particles with a basic sugar or oligonucleotide produces changes in the surface energy and other surface characteristics which make the particles well suited for attachment of biologically active moieties or other members of a BRP.

WO 95/12392 PCT/US9~/12515 ln accordance with the present invention, ultrafine core particles having diameters of less than about 1000 nanometers are used to anchor enzymes or other catalytic particles without denaturing the catalyst.
Surface coating of the core particles provides an anchoring surface which prevents substantial alteration of the catalysts which might otherwise occur when the catalysts are attached directly to the particle surface. The coated particles are also useful for anchoring catalyst-substrate (enzyme-substrate) pairs or other bioreactive pairs without destroying the catalytic activity of the BRP's.
The present invention also involves the attachment of BRP's to macroscopic surfaces, such as films or solid surfaces. Anchoring of BRP's to these type of large surface area systems are useful where chemical catalysis or cell receptor activation is not dependent upon or does not require the microgeometry provided by nanocrystalline core anchoring 1 5 particles.
As a further aspect of the present invention, biologically active peptides, proteins or pharmacological agents are attached to a biodegrad-able core particle to provide a wide variety of biologically active composi-tions. The invention is based in part on the discovery that the surface of ultrafine particles (nanocrystalline particles) can be modified with a surface coating to allow attachment of biologically active moieties to produce compositions wherein the naturally occurring structural environment of the moiety is mimicked sufficiently so that biological activity is preserved. The core particle, with the surface coating and attached moiety, is further coated with a targeting agent, such as a biologically active ligand or phospholipid membrane complex.
The invention is also based in part on the discovery that biodegrad-able core particles may be assembled with biologically active or pharma-ceutical agents to form a biologically active core which is further treated ~ WO 95/12392 ~ ~ PCT/US94/12515 _9_ with a targeting ligand or membrane complex to provide selective targeting of the biochemically active core in vivo.
As a feature of the present invention, the nanocrystalline core is composed of brushite which is composed of crystalline calcium phosphate (brushite). This material is biodegradable, inexpensive and is found in human beings as a substrate of bone synthesis. The brushite particles may be synthesized at a nanomeric size (between approximately 5 nm and 150 nm). This small size allows the drug delivery construct to be small enough to avoid uptake by the Reticulo-Endothelial System (RES) of the body and deliver the drug or biologically active agent in vivo without non-specific toxicity or loss of drug to macrophages.
The brushite core may be co-crystallized with a drug or agent for sustained release and protection of the drug from the physiological environment. Conversely, the brushite core may be coated, as described above with a sugar, such as cellobiose or pyridoxal-phosphate to improve its surface attachment characteristics. The sugar coat allows for the binding of drugs and other biological agents in their active or inactive forms, without alteration. The sugar helps to maintain the conformation of the drug or agent that is adsorbed to its surface. This core construct is capable of carrying a moderate drug or agent load, depending upon the size of the biological agent.
As a feature of the present invention, the brushite based particle is targeted to a specific tissue or cell type. In order to achieve this targeting, the construct has a targeting ligand or a primed phospholipid membrane tightly adsorbed to its surface. The membrane may contain proteins, receptors and carbohydrates which provide targeting of the vehicle. The membrane also serves to further maintain the stability of the biological agent and the integrity of the construct. This membrane may be derived from cell membranes, viral envelopes (see U.S. patent 5,178,882), or other specifically engineered or synthesized membranes. Due to the very small size of the biodegradable core particle deiivery system, multiple layers of membranes may be adsorbed to the core particle to increase the efficiency of targeting.
As one aspect of the present invention, biodegradable nanocrystal-line particles are used to prepare a decoy virus wherein the DNA or RNA
core of the virus is replaced by the microparticle. The microparticle is chosen to be approximately the same size as the viral core so that the conformation of the surrounding protein coat accurately mimics the native virus. The resulting viral decoy is incapable of infectious behavior while at the same time being fully capable of effecting an immune response and otherwise being antigenically bioreactive.
The biologically active microparticles in accordance with the present invention have wide-ranging use depending upon the type of biologically active compound which is attached to the biodegradable microparticle core.
When viral protein from HIV is attached to the microparticle core, the result is a decoy virus which may be used as an AIDS vaccine, diagnostic tool or antigenic reagent for raising antibodies. Non-viral protein or antigen coatings may be selected and structured for use in raising specific anti-bodies or as a diagnostic tool. Further, the microparticles can function as a pharmacological agent when compounds having pharmacological activity are attached to the biodegradable core particle.
In accordance with the present invention, biodegradable core parti-cles having diameters of less than about 1000 nanometers are used to anchor enzymes or other catalytic particles without denaturing the catalyst.
Surface coating of the core particles provides an anchoring surface which prevents substantial alteration of the catalysts which might otherwise occur when the catalysts are attached directly to the particle surface. The coated particles are also useful for anchoring catalyst-substrate (enzyme-substrate) pairs or other bioreactive pairs (BRP's) without destroying the catalytic activity of the BRP's.

WO 95/12392 PCT/US9~/12515 1 7~

As an additional aspect of the present invention, transfecting DNA
or RNA is attached to a nanocrystalline core particle and coated with a targeting ligand or membrane to provide a viral transfection system which may be used in gene therapy. The invention is based in part on the discovery that the surface of ultrafine particles (nanocrystalline particles) can be modified with a surface coating to allow attachment of transfecting DNA or RNA to produce cornpositions wherein the naturally occurring structural environment of the DNA or RNA is mimicked sufficiently so that biological activity is preserved. The core particle, with the surface coating and attached transfecting DNA or RNA, is further coated with a targeting agent, such as ligand or phospholipid membrane complex to provide target-ing of the DNA or RNA to particular cell receptors.
As a feature of this aspect of the present invention, the nanocry-stalline core may be composed of brushite. This material is biodegradable, inexpensive and is found in human beings as a substrate of bone synthesis.
The brushite particles may be synthesized at a nanomeric size (between approximately 5 nm and 150 nm). This small size allows the DNA/RNA
delivery construct to be small enough to avoid uptake by the Reticulo-Endothelial System (RES) of the body and deliver the DNA/RNA to cells in vivo without non-specific toxicity or loss of drug to macrophages.
The DNA/RNA-particle construct is targeted to a specific tissue or cell type. In order to achieve this targeting, the construct has a targeting ligand or a primed phospholipid membrane tightly adsorbed to its surface.
The membrane may contain proteins, receptors and carbohydrates which provide targeting of the vehicle. The membrane also serves to further maintain the stability of the transfecting DNA or RNA and the integrity of the construct. This membrane may be derived from cell membranes, viral envelopes (see U.S. patent 5,178,882), or other specifically engineered or synthesized membranes. Due to the very small size of the biodegradable core particle delivery system, multiple layers of membranes may be WO 9SI12392 PCT/US91/12515 ~
21~ 4~

adsorbed to the core particle to increase the efficiency of targeting. The DNA/RNA transfecting microparticles in accordance with the present inven- t tion have wide-ranging use depending upon the particular DNA or RNA
which is attached to the biodegradable microparticle core.
The above-discussed and many other features and attendant advan-tages of the present invention will become better understood by reference to the following detailed description.

DETAILED DESCRIPTION OF THE INVENTION
The present invention has wide application to immunologic proce-dures and methods wherein antigenic material, biologically reactive pairs (BRP's) or other biologically active moieties are utilized. These areas of application include vaccination agents, antigen agents used to raise anti-bodies for subsequent diagnostic uses and antigenic compounds used as diagnostic tools. The composition of the invention can also be used in a wide variety of other applications where there is a need to target pharma-ceuticals or other biologically active agents to particular cell types in both in vivo and in vitro environments.
The compositions of the present invention include biodegradable nanocrystalline core particles (diameters of less than 1000 nm) which are coated with a surface energy modifying layer that promotes bonding of proteins, peptides or pharmaceutical agents to the particles. The coating modifies the surface energy of the nanocrystalline core particles so that a wide variety of immunogenic proteins, peptides and pharmaceutical agents may be attached to the core particle without significant loss of antigenic activity or denaturation. The result is a biologically active composition which includes a biologically inert core. The end use for the compositions of the present invention will depend upon the particular protein, peptide or pharmacological agent (including gene therapy) which is attached to the coated core particle. For example, proteins or peptides having antigenic W09511239~ 7~ PCl 1594/1251 activity may be attached to provide compositions useful as immunodiag-nostic tools. Viral fragments or protein coatings having immunogenic activity may be attached to provide a vaccine. Also, pharmacological agents may be attached to provide compositions which are useful in treat-ing diseases. Also, gene segments or antisense fragments may be attached which are useful in treating diseases.
Examples of individual catalysts which may be attached to the coated core particles include tissue plasminogen activator (whole and partial domains), trypsin inhibitor, cytrochromes, Ferredoxin, phospho-transferase, acyltransferase, papain, Lys C, Arg C, Trypsin, Coagulation factor V, Xlla, Xla, Vlla, Complement factor C3, C3b and properdin.
Bioreactive pairs (BRP's), such as enzyme-substrate pairs, may also be attached to the coated core particles. Exemplary enzyme-substrate pairs include: Iysozyme-chitin pairs, where the Iysozyme catalyzes the hydrolysis of NAM and NAG glycosidic bonds; ribonuclease-RNA pairs where the ribonuclease catalyzes the hydrolysis of RNA; carboxypeptidase A carboxyl terminal polypeptide pairs where the enzyme catalyzes the hydrolysis of the carboxyl-terminal peptide bond in the polypeptide chain;
serine, zinc, thiol and carboxyl proteases-protein pairs where the protease catalyze the degradation of the protein; NADH-Q reductase - NADH
pairs where the reductase catalyzes the oxidation of NADH and the reduc-tion of Q; glutathione seductase-glutathione pairs; acetylcholinesterase-acetylcholine pairs; Lys C; Arg C; acyl and acyltransferase; aspartate and aspartate carbamoyltransferase; elastase; and the cytochromes.
Other biochemically reactive pairs (BRP) which can be immobilized on to the coated solid surfaces in accordance with the present invention include members of immunological pairs, ligand-receptor pairs, drug-recep-tor pairs, catalyst reactant pairs, catalyst-substrate pairs, absorbate-absorbent pairs, adsorbate-adsorbent pairs, and toxin-ligand pairs. Such members include but are not limited to:

W O 95/12392 PCTAUS94/12515 ~
2~

immunological pair members such as IgG, IgM, IgA, IgE and IgD, whole or in part as in Fc or Fab fractions, polyclonal or monoclonal, with recognition sites for epitopes on cells (cell surface antigens) such as CD1, CD3, CD4, CD8, CD11, CD25, CD68; viral epitopes such as EBVgp350, HlVp24, HlVgp120, MS virus coat protein (bacteriophage) other viral antigens, bacterial antigens, fungal antigens, and known viruses, fungi, bacteria, prions and protozoa.

Iigand-receptor pair members such as lectins and lectin binding sites such as FVIII receptor; HDL and HDL receptor cellular receptor site;
hormones such as estrogen and estrogen receptor sites; antibiotics;
ribosomal proteins; FK506 and FK506 binding protein; ricin and cell target; phosphotyrosine recognition domain SH2 (RSV) and phos-photyrosine; and (oligo)nucleopeptides and their corresponding antisense nucleopeptide.

drug-receptor pair members such as epinephrine and adrenergic receptors, methadone and opiate receptors, DNA chelating agents such as adriamycin, etc.
catalyst-reactant pair members such as iron and superoxide, rhodopsin kinase and rhodopsin, hydrogen peroxide and luminol, horseradish peroxidase and hydrogen peroxide.

adsorbent-adsorbate pair members, such as trypsin-trypsin inhibitor, biotin-biotin repressor (E. coli) and subtilisin and subtilisin inhibitor.

toxin-ligand pair members such as strychnine and the glycine receptor, hemoglobin and carbon monoxide, and organophosphate compounds (sarin, tabun, parathion, dimefox, malathion, diazinon) WO95/12392 1 7~2q~ PCT/US94/12515 and acetylcholinesterase; muscarinic receptor and neurotoxins (Neurotoxin I from S. helianthus' scorpion neurotoxin); verotoxin and colonic mucosal epithelial receptor; enterotoxin and colonic mucGsal epithelial receptor.

One or both of the members of the BRP may be initially bound to the modified surface. In general, the enzyme or catalyst will be bound first and substrate or reactant bound later during actual interaction between the enzyme and substrate or catalyst and reactant.
10For preparing decoy viruses for use as vaccines, particles having diameters of between about 10 to 200 nanometers are preferred since particles within this size range more closely mimic the diameter of DNA
and RNA cores typically found in viruses.
The core particles used to anchor catalysts or BRP's can have a 15much broader size range than the particles used in decoy viruses. The particle size should be chosen to maximize the catalytic or enzymatic reac-tion of the BRP. Preferred particle sizes are in the range of 50 to 150 nm.
If desired, the BRP may be attached to a macroscopic surface, such as a film or solid substrate surface.
20The core particles or other surfaces may be made from a wide variety of inorganic materials including metals or ceramics. Preferred metals and alloys include beryllium, silicon, gallium, copper, gold, titanium, nickel, aluminum, silver, iron, steels, cobalt-chrome alloys, and titanium alloys. Preferred ceramic materials include calcium-phosphate, alumina, 25silica, and zirconia. The core particles may be made from organic materials including carbon (diamond). Preferred polymers include polystyrene, sili-cone rubber, polycarbonate, polyurethanes, polypropylenes, polymethyl-methaacrylate, polyvinyl chloride, polyesters, polyethers, and polyethylene.
The core particles may be made from a variety of biodegradable 30materials including polymers or ceramics. Preferred ceramic materials WO 95/12392 PCT/US94/12515 ~

2~ 2~ -16-include brushite composed of calcium phosphate dihydrate, and tricalcium phosphate and iron oxide. Preferred biodegradable polymers include polylactide, polygalactide and polylysine. Particles made from brushite are particularly preferred.
Particles made from the above materials having diameters less than 1000 nanometers are available commercially or they may be produced from progressive nucleation in solution (colloid reaction), or various physical and chemical vapor deposition processes, such as sputter deposition (Hayashi, C., J. Vac. Sci. Technol. A5 (4), Jul/Aug.1987, pgs.1375-1384; Hayashi, C., Physics Today, Dec.1987, pgs. 44-60; MRS Bulletin, Jan 1990, pgs.
16-47). Tin oxide having a dispersed (in H2O) aggregate particle size of about 140 nanometers is available commercially from Vacuum Metallurgical Co. (Japan). Other commercially available particles having the desired composition and size range are available from Advanced Refractory Technologies, Inc. (Buffalo, N.Y.).
Plasma-assisted chemical vapor deposition (PACVD) is one of a number of techniques that may be used to prepare suitable microparticles.
PACVD functions in relatively high atmospheric pressures (on the orde~ of one torr and greater) and is useful in generating particles having diameters of up to 1000 nanometers. For example, aluminum nitride particles having diameters of less than 1000 nanometer can be synthesized by PACVD
using Al (CH3)3 and NH3 as reactants. The PACVD system typically includes a horizontally mounted quartz tube with associated pumping and gas feed systems. A susceptor is located at the center of the quartz tube and heated using a 60 KHz radio frequency source. The synthesized alumi-num nitride particles are collected on the walls of the quartz tube.
Nitrogen gas is used as the carrier of the Al (CH3)3. The ratio of Al (CH3)3:
NH3 in the reaction chamber is controlled by varying the flow rates of the N2/AI(CH3)3 and NH3 gas into the chamber. A constant pressure in the reaction chamber of 10 torr is generally maintained to provide deposition W095/12392 21 7~ PCT/US94/12515 and formation of the ultrafine nanocrystalline aluminum nitride particles.
PACVD may be used to prepare a variety of other suitable nanocrystalline particles.
The core particles or other surface are coated with a substance that provides a threshold surface energy to the particle or other surface which is sufficient to cause binding to occur without that binding being so tight as to denature biologically relevant sites. For particles, coating is prefer-ably accomplished by suspending the particles in a solution containing the dispersed surface modifying agent. It is necessary that the coating make the surface of the particle more amenable to protein or peptide attachment.
For surfaces, the coating may be applied to a meticulously clean area located on the surface.
Suitable coating substances in accordance with the present invention include carbohyd rates, carbohydrate derivatives, and other macromolecules with carbohydrate-like components characterized by the abundance of -OH
(hydroxyl) side groups. The coatings may include but are not limited to:
short chain carbohydrates including glucose, sucrose, cellobiose, nystose, triose, dextrose, trehalose, glucose, lactose, maltose, etc.

hydroxyl rich weak acids such as citrate, fumarate, succinate, isocitrate, oxaloacetate, malate, etc.

nucleotide-like molecules with pendant carbohydrate or phosphate groups such as pyridoxyl-5-pyrophosphate,thiamine pyrophosphate, uridine-diphosphate-glucose, glucose-1-phosphate, adenosine, nicotinamide-adenine-diphosphate, etc.

derivatives of carbohydrates such as nitrocellulose WO 95/12392 PCT/US9~/12515 ~

2~71~ 4A -18-complex polymeric carbohydrates and derivatives such as dextran, glycogen, etc.

Preferred coating materials include cellobiose, sucose, pyridoxyl-5-phosphate and citrate.
An exemplary preferred method for binding the stabilizing coat to the solid phase followed by a member of a BRP consists of:
1. obtaining a meticulously clean surface on the solid to be coated;

2. immersion of the meticulously clean surface to be coated in an aqueous solution of the coating material followed by;

3. Iyophilization of the aqueous solvent/dispersant from the surface of the solid;

4. immersion of the coated solid surface in an aqueous solution/disper-sion containing (a) member(s) of a biochemically reactive pair (BRP);
and 5. removal of the aqueous solvent yielding a solid coated with a molecular stabilizing film to which is bound (a) BRP member(s).

As used in Step 1 above, the term "meticulously clean surface"
means a surface of a material that has been cleansed of all matter that is not intrinsic to the material comprising the bulk of the solid to be coated.
If a solid of some composition A is already coated with a second solid of some composition B, and it is desired that the molecular stabilizing film be applied to the surface of composition B, then the term refers to surface B
and matter that is not intrinsic to the material comprising the bulk of B.
Such techniques include the individual or combined application of acids, ~ WO 9~/12392 PCT/US94/12515 ~1 7~

bases, sonic energy, plasma glow discharge processes, and even mechani-cal cleansing to preformed surfaces.
Immersion, as used in Step 2 above, means the application of a con-tiguous layer of the solution to the surface undergoing coating. Such techniques as spraying, dipping, mechanical painting, or other means of transfer are intended insofar as they yield a contiguous layer comprised exclusively of the solvent and coating macromolecule on the surface to be coated.
Lyophilization, as used in Step 3 above, means the removal of the aqueous phase from the surface film by a reduction in the ambient gas partial pressure. Both the application of heat and the removal of heat to cool the solid and the newly forming surface film may be modifications of the Iyophilization process.
Immersion, as used in Step 4 above, means the application of a contiguous layer of a solution containing (a) member(s) of a BRP to the already modified surface of the coated solid. Such techniques as spraying, dipping, mechanical painting, or other means of transfer are implied insofar as they yield a contiguous layer comprised exclusively of the solvent and BRP members on the already modified surface consisting of a molecular stabilizing film to be coated.
Removal, as used in Step 5 above, means the removal of the aque-ous phase from the surface film by (a) a reduction in the ambient gas partial pressure (Iyophilization) or (b) dialysis/ultrafiltration. Both the appli-cation of heat and the removal of heat to cool the solid and the newly forming surface film may be modifications of the Iyophilization process.
With respect to particles, the particles are suspended in a coating solution. The coating solution into which the core particles are suspended contains, for example, from 1 to 30 weight/volume percent of the coating material. The solute is preferably double distilled water (ddH20). The amount of core particles suspended within the coating solution will vary WO 95/12392 PCTIUS94112515 ~
2 4 ~

depending upon the type of particle and its size. Typically, suspensions containing from 0.1 to 10 weight/volume percent are suitable. Suspen-sions of approximately 1 weight/volume percent of particles are preferred.
The core particles are maintained in dispersion in the coating solution for a sufficient time to provide uniform coating of the particles. Sonication is the preferred method for maintaining the dispersion. Dispersion times ranging from 30 minutes to a few hours at room temperature are usually sufficient to provide a suitable coating to the particles. The thickness of the coating is preferably less than 5 nanometers. Thicknesses of the coating may vary provided that the final core particles include a uniform coating over substantially all of the particle surface.
The particles are separated from the suspension after coating and may be stored for future use or redispersed in a solution containing the protein or peptide to be attached to the particles. Alternatively, the coated particles may be left in the suspension for further treatment involving attachment of the desired protein or peptide.
The term "biodegradable" as used herein means any core particle which decomposes or otherwise disintegrates after prolonged exposure to a mammalian in vivo environment. To be biodegradable, the core particle should be substantially disintegrated within a few weeks after introduction into the body.
The protein or peptide which is applied to the coated particles may be selected from a wide variety of proteins or peptides. Those having antigenic properties are preferred when a vaccine is required. The protein can be the viral protein coat from a selected virus or immunogenic portion thereof. The viral protein coat is isolated according to known separation procedures for isolating and separating viral proteins. The viral coating is the preferred protein because the viral coating is where the antigenic activity of viruses is known to be located. Typically, the virus is digested or solubilized to form a mixture of viral proteins. The viral proteins are then - =

separated by liquid chromatography or other conventional process into the various protein particle fractions and dialyzed to remove impurities.
Suitable viruses from which viral protein particles can be separated and isolated include Epstein-Barr virus, human immunodeficiency virus (HIV), human papilloma virus, herpes simplex virus and pox-virus. Prepara-tions of a wide variety of antigenic protein materials may also be purchased commercially from supply houses such as Microgene Systems, Inc. (400 Frontage Road, West Haven, Connecticut 06516), Amgen Corporation (1900 Oak Terrace Lane, Thousand Oaks, California 91320-1789) and Cetus Corporation (1400 53rd Street, Emeryville, California 94608 and Advanced Biotechnology, Inc. (Columbia, Maryland). Synthetic peptides and/or proteins which correspond to naturally occurring viral particles may also be utilized.
With respect to HIV, any of the viral fragments which are known to elicit an immune response can be used. Suitable viral fragments include gp120, gp160, gp41, and core proteins (p24). Any of the known tech-niques for preparing HIV fragments may be used including recombinant methods.
Other biologically active proteins and peptides that can be attached include enzymes, hormones, transport proteins and protective proteins Human serum transferrin, plasminogen activator and coagulation factors, in addition to the pharmacologic agents amphotericin, taxol and insulin, are examples.
The procedure for attaching the antigens or other protein to the coating on the core particles involves suspending the coated core particles in an aqueous solution containing the antigen. The presence in the solution of materials that may preferentially attach to the particle surface is often not advantageous. For example, the dispersion agents present in the solu-tion may create an undesirable coating on the suspended particles prior to protein attachment. Water miscible solvents such as methanol or ethanol WO 95/12392 PCT/US94/12515 ~

æ ~7 ~ ~4 -22-may be used. The aqueous solution of coated microparticles can be agi-tated sufficiently to provide a uniform suspension of the particles.
Typically, the amount of particles in solution will be between about 0.5 mg per milliliter of solution and 5 mg per milliliter of solution. Sonication is a preferred method for providing a uniform suspension of the coated particles in solution.
The suspension of coated particles and antigens must be within cer-tain parameters for protein attachment and self assembly to occur. The temperature of the particle solution should be between 1 C to 45C. Cer-tain proteins and pharmaceutical agents may be bound to the coated parti-cles in distilled water. Salts may be added to the solution for reactions between coated particles and proteins and other pharmaceutical agents which are unstable or will not disperse readily in distilled water. In general, the salt solutions should be formulated so that the ionic balance (in mM) does not exceed: K=300-500; Na=30-70; Cl=40-150; Ca--0.0003-0.001; and Mg=0.0003-0.001. The oxygen tension of the solution is, advantageously, less than 10% in a solution sparged initially by helium and then gassed with helium, nitrogen and carbon dioxide. The pH of the solu-tion is, advantageously, slightly acidic (relative to blood), with a value, preferably, of between 6.8 to 7.2. An exemplary solution for dispersion of the coated microparticles and for protein attachment is an aqueous solu-tion containing: 0.0360 milligrams MgSo4 per liter, 0.0609 milligrams MgCI26H20, 0.0441 milligram CaCI2.2H20, 22.823 grams K2HP04,13.609 grams KH2P04,7.455 grams KCI, and 4.101 gram sodium acetate. The pH
of this solution is adjusted to 6.8.
The coated particle cores with the attached protein can be separated from the ionic growth medium and stored for further use. The coated particles may be stored by any of the conventional methods typically used for storing antigenic compounds or antibodies. For example, the coated particles may be freeze dried (Iyophilized) or stored as a suspension in a * WO 9~112392 PCT/US94/12515 ~1 7~2~- 23 compatible solution. When used as a vaccine, the particles coated with a viral protein coat are injected or otherwise administered to the individual according to conventional procedures. Any pharmaceutically acceptable carrier solution or other compound may be used in administering the coated particles to the individual. When used for diagnostic purposes in vitro, the protein coated particles are suspended in solution and used in the same manner as other antigenic compounds. The same is true for use of the protein coated particles for raising antibodies. The same protocol and procedures well known for using antigens to produce antibodies may be used wherein the protein coated particles of the present invention are substituted for normally used antigenic compounds.
When targeting of the coated particle and attached biologically active agent is desired, the particles may be coated with a target ligand or a phospholipid membrane complex which is reactive with receptors on par-ticular cells. Exemplary target ligands include HIV coat proteins (gp160, 41, 120) corona virus coat proteins, EBV coat proteins (gp350). Any membrane bound ligand/receptor may be used. These ligands are attached to the particle complex in the same manner as attachment of the biolo-gically active agents discussed above.
The lipids used to coat the b~odegradable nanocrystalline particle and bound agent may also be the same lipids commonly used to form lipo-somes. Suitable lipids include phospholipids such as phosphatidylcholine, cholesterol and phosphatidylserine. The lipids may also be derived directly from natural sources. Such lipids include viral membranes and other lipid bound biochemically reactive pairs. The lipid layer is applied to the nanocrystalline core particle and bound to a biologically active agent in the same manner as the surrogate red blood cells described in United States Patent No. 5,306,508.

WO 95J12392 PCT/US94/12Sl~i ~

2~ ~2~ -24-The core particle and bound agent do not need to be totally covered with a lipid layer. Preferably, the amount of lipid used to coat the particle will be sufficient to coat the entire particle.
In certain situations, it is desirable to attach the biochemically active agent directly to the biodegradable core particles. This direct attachment is preferably accomplished by co-crystallizing the biodegradable core parti-cle with the biochemically active agent. For example insulin, taxol or (DNA,RNA) gene fragments may be co-crystallized with brushite to form drug loaded particles which provide sustained release of the drug while providing protection of the drug in vivo. This direct attachment reduces biological activity.
The present invention also has wide application to procedures and methods wherein DNA or RNA are delivered, i.e. transfected, to cells in vivo or in vitro. These areas of application include gene therapy. The compositions of the present invention can be used in a wide variety of other applications where there is a need to target DNA orRNA to particular cell types in both jn vivo and in vitro environments. The invention may also be used to target other transfection agents, such as antisense frag-ments. The term "transfection agent" as used herein is intended to mean DNA or RNA segments or antisense fragments which are capable of trans-fection into a cell. Exemplary transfection agents include sense DNA, sense RNA, antisense RNA, and antisense DNA.
The compositions of the present invention include nanocrystalline core particles (diameters of less than 1000 nm) which are coated with a surface energy modifying layer that promotes bonding of proteins, peptides or pharmaceutical agents to the particles. The coating modifies the surface energy of the nanocrystalline core particles so that DNA and RNA seg-ments may be attached to the core particle without significant loss of acti-vity or denaturization. The result is a biochemically active composition which includes a biochemically inert core. The end use for the composi-~ WO 95112392 PCT/US94112515 ~1 7~2~ 25 tions of the present invention will depend upon the particular transfection agent which is attached to the coated core particle. For example, DNA
segments, such as human low density lipid receptor, are used in gene therapy. RNA segments, such as antisense and sense MRNA, are used in the transfection procedure. Antisense fragments, such as HIV reverse transcriptase are used in the transfection particles. Preferred particle sizes are on the order of 10 nm to 1 5Q nm.
The core particles and coatings are the same as described above.
The particles are separated from the suspension after coating and may be stored for future use or redispersed in a solution containing the DNA or RNA to be attached to the particles. Alternatively, the coated particles may be left in the suspension for further treatment involving attachment of the desired DNA or RNA.
The core particle may be made from a biodegradable ceramic or polymer. The term "biodegradable" as used herein means any core particle which decomposes or otherwise disintegrates after prolonged exposure to a mammalian in vivo environment. To be biodegradable, the core particle should be substantially disintegrated within a few weeks after introduction into the body. Brushite is a preferred biodegradable core particle material.
The DNA or RNA which is applied to the coated particles may be selected from a wide variety of DNA or RNA segments which are used to transfect cells during gene therapy. Antisense fragments may also be used.
Exemplary transfection gene segments include human low density lipid receptor CDNA, human adenosine deaminase CDNA, human dystro-phan CDNA, and antisense HIV reversed DNA--all subcloned within appropriate expression vectors. The DNA or RNA transfection segments may be prepared according to known procedures such as the procedure described in Manniatis, T., Fritsch, E.F., and Sambrook, S., Molecular Cloning, Cold Spring Laboratory Press, N.Y., 1.0-19.0 (1989). Gene W0 95/12392 ~ PCT/US94/12515 segments are also available commercially from a number of different suppliers .
The procedure for attaching gene segments or antisense fragments to the coating on the core particles involves suspending the coated core particles in an aqueous solution containing the gene segments. The presence in the solution of materials that may preferentially attach to the particle surface is often not advantageous. For example, the dispersion agents present in the solution may create an undesirable coating on the suspended particles prior to protein attachment. Water miscible solvents such as methanol or ethanol may be used. The aqueous solution of coated microparticles can be agitated sufficiently to provide a uniform suspension of the particles. Typically, the amount of particles in solution will be between about 0.5 mg per milliliter of solution and 5 mg per milliliter of solution. Sonication is a preferred method for providing a uniform suspension of the coated particles in solution.
The suspension of coated particles and gene segments must be within certain parameters for segment attachment and self assembly to occur. The temperature of the particle solution should be between 1 C to 45C. The gene segments or antisense fragments may be bound to the coated particles in distilled water. The oxygen tension of the solution is preferably less than 10% in a solution sparged initially by helium and then gassed with helium, nitrogen and carbon dioxide. The pH of the solution is preferably slightly acidic (relative to blood), with a value, preferably, of between 6.8 to 7.2. An exemplary solution for dispersion of the coated microparticles and for DNA attachment is an aqueous solution containing:
0.0360 milligrams MgSo4 per liter, 0.0609 milligrams MgCI2 ~H20, 0.0441 milligram CaCI2 2H20, 22.823 grams K2HP04, 13.609 grams KH2P04, 7.455 grams KCI, and 4.101 gram sodium acetate. The pH of this solution is adjusted to 6.8.

21 712~

The coated particle cores with the attached gene segment or anti-sense fragment can be separated from the ionic growth medium and stored for further use. The coated particles may be stored by any of the conven-tional methods typically used for storing gene segment or antisense fragments. For example, the coated particles may be freeze dried or stored as a suspension in a compatible solution. When used in gene therapy, the particles coated with a targeting layer as described below, are injected or otherwise administered to the individual according to conventional proce-dures. Any pharmaceutically acceptable carrier solution or other compound may be used in administering the DNA/RNA coated particles to the indivi-dual. When used in vitro, the DNA/RNA coated particles are suspended in solution and used in the same manner as other gene therapy compounds.
The same is true for use of antisense coated particles. The same protocol and procedures well known for gene therapy to introduce genes into cells both in vivo and in vitro may be used wherein the DNA/RNA/antisense-particle constructs of the present invention are substituted for other gene therapy compounds.
Targeting of the coated particle and attached gene support or anti-sense fragment is accomplished by coating the particles with a phospho-lipid membrane complex which includes ligands that are reactive with receptors on particular cells. Exemplary target ligands include HIV coat proteins (gpl 60, 41, 120) corona virus coat proteins, EBV coat proteins (gp350). Any membrane bound ligand/receptor may be used. These ligands are attached to the particle complex in the same manner as attach-ment of the transfection agents discussed above.
The lipids used to coat the biodegradable nanocrystalline particle and bound transfection agent are the same lipids commonly used to form lipo-somes. Suitable lipids include phospholipids such as phosphatidylcholine, cholesterol and phosphatidylserine. The lipid layer is applied to the nano-crystalline core particle and bound to a biologically active agent in the same WO 95/12392 PCT/US94/12S15 ~

~4~ 28-manner as the other coatings are applied, i.e. by adsorption onto the surface.
The core particle and bound agent do not need to be totally covered with a lipid layer. Preferably, the amount of lipid used to coat the particle will be sufficient to coat the entire particle. The combined layer of lipid and targeting ligand provide for targeting of the core particle and attached gene segments to the corresponding cell receptor.
The preferred transfection nanoparticles are self-assembling com-piexes of nanometer sized particles, typically on the order 100 nm, that carry an inner layer of transfecting DNA or RNA and an outer layer of targeting molecules. Functionally, the targeting molecules, usually referred to as ligands, impart tissue specificity in the same way a virus finds its host, i.e., the ligands promote the association of the transfection nano-particles to a cell surface by binding to cell surface receptor molecules.
Construction of the transfection nanoparticles is a simple process and which occurs spontaneously without apparent covalent modification.
In one exemplary synthesis, nanoparticle dispersions of tricalcium phos-phate (TCP) are prepared from isochoric opposing streams of 0.750 M
Calcium Chloride and 0.25 M monobasic Sodium Phosphate. The resulting precipitant is sonicated at 175 Watts at room temperature for 30 minutes and washed in volumes of 20 mM pH 6.80 phosphate buffer before being mixed with Cesium Chloride purified transfecting DNA, RNA or antisense.
The DNA, RNA or antisense is then left to adsorb to the particulate surfaces at room temperature under mild agitation. After the transfection agent attachment step is completed, membrane specific ligands, typically prepared from the envelopes of retroviruses, are added to the dispersion and allowed to adsorb overnight at 4.0C in a stir cell.
The ligand receptor complex can be chosen so as to be unique for the targeted tissue since tissues can be differentiated by their component cells, cell surface receptors, and complementary ligands. Once the interac-~ -29-tion takes place, transfection can proceed through a range of cellular uptake mechanisms, resulting in the dissolution of the DNA (RNA/anti-sense) away from the particle complexes, recombination, and expression in a target cell. Introducing DNA, RNA or antisense by this method allows for the alteration of the phenotype of specific cells in a targeted tissue. It will occur because of the conformational stability of the ligands employed, the integration locus of the transfecting DNA/RNA/antisense, and the expression ability of transfecting DNA (RNA/antisense) in the targeted tissue.
Ligand preparation is as varied as their source. They can be produced by recombinant means or derived from their natural source.
Preferably, viral envelope ligands are extracted from viruses, such as Human Immunodeficiency Virus, Epstein-Barr Virus, and murine ecotropic viral strains. In general, the viral envelopes are extracted in accordance with known procedures and then combined with phospholipids in a phos-phate buffer. The solution of viral envelopes and phospholipids are then added to the suspension of DNA/RNA coated nanoparticles. The viral envelopes and phospholipids are absorbed onto the nanoparticles to form a targeting membrane.
Typical preparations of transfection nanoparticles yield in the neighborhood of a tenth of a microgram of DNA (and RNA) per microliter of dispersion as gauged by spectrophotometric determinations over time.
If higher concentrations are required, the DNA (and RNA) is premixed with the substrate solutions and is allowed to slowly coprecipitate with the core material at a pH of 6.5. The particulate size is controlled by the time wise addition and surface adsorption of membrane ligands, and by the removal of substrate by ultrafiltration dialysis. Independent of the synthetic route chosen, administration of the transfection nanoparticles is accomplished by both enteral and parenteral routes. The doses are the same as those used in gene therapy.

The nanoparticle-DNA/RNA constructs of the present invention may be made without the targeting layer when receptor mediated targeting is not required. For example, the constructs may be prepared without the targeting layer when calcium channel uptake of DNA/RNA or other non-cell receptor uptake is desired. The small size of the constructs allows them to evade the reticular endothelial system, thereby increasing the circulation time and transfection efficiency.
The following non-limiting examples describe certain aspects of the present invention in greater detail.
Example 1. PreDaration of nanocrystalline tin oxide micro,~articles:
1.5 to 2.0 mg of ultrafine (nanocrystalline) metal powder was placed in a 1.7 ml screw-cap microcentrifuge with 1.5 mls of double distilled water (ddH20). The ddH20 was filtered through a rinsed 0.45 micron filter-sterilizing unit or acrodisc (Gelman Scientific). The metal powder was tin oxide with a mean diameter (by photon correlation spectroscopy) of 140 nm. The mixture was vortexed for 30 seconds and placed into a water sonicating bath overnight. The sonication bath temperature was stabilized at 60C. After a 24-hour sonication, the samples were vortexed once more for 30 seconds with the resulting dispersion clarified by microcentrif-ugation at approximately 16,000 rpm for 15 seconds. The analysis of particle size was carried out on a Coulter N4MD sub-micron particle analyzer.
The coating was applied to the tin oxide particles by suspending the particles in a stock solution of cellobiose. The cellobiose stock solution was a 292 mM solution made by dissolving 1.000 gram of cellobiose in 9.00 mls of ddH20. Solution was accomplished at approximately 70C in order to promote quick dissolution. The resulting cellobiose solution was filter sterilized through a rinsed 0.45 micron filter with the final volume being adjusted to 10.00 ml.

~ WO 95/12392 PCT/US94/12515 ~2~ -31 -Sufficient cellobiose stock solution was added to 150 microliters of ultrafine tin oxide dispersion so that the final concentration of the tin oxide was 1.00 percent (w/v) or 29.2 mM. A typical volume for preparation was 2.0 mls which was mixed four or five times by the action of a micro-pipetor. After mixing, the dispersion was allowed to equilibrate for two hours. Demonstration of successful coating of the particles was provided by measuring the mobility of the particles (coated and uncoated) on a Coulter DELSA 440 doppler energy light scatter analyzer. The coated tin oxide particles exhibited a relatively low mobility compared to the non-coated tin oxide particles. Measurements were also taken at various dilute salt concentrations to ensure that the observations with respect to mobility were not artifactual. The tests demonstrate that the particles were coated with the cellobiose.
The coated particles are then used to attach antigenic proteins, peptides or pharmacological agents to prepare bioreactive particles.

Example 2. Prenaration of nanocrvstalline ruthenium oxide Darticles:
~he same procedure was carried out in accordance with Example 1, except that ruthenium oxide microparticles were substituted for the tin oxide particles. The ruthenium oxide particles were obtained from Vacuum Metallurgical Company (Japan).

Example 3. Precaration of the nanocrystalline silicon dioxide and tin oxide narticles: Nanocrystalline silicon dioxide was acquired commercially from Advanced Refractory Technologies, Inc. (Buffalo, N.Y.) and tin oxide was acquired commercially from Vacuum Metallurgical Co. (Japan). The tin oxide particles were also prepared by reactive evaporations of tin in an argon-oxygen mixture and collected on cooled substrates. Nanocrystalline tin oxide was also synthesized by D.C. reactive Magnetron sputtering (inverted cathode). A 3" diameter target of high purity tin was sputtered 2~7g~24~ 32 in a high pressure gas mixture of argon and oxygen. The ultrafine particles formed in the gas phase were collected on copper tubes cooled to 77K
with flowing liquid nitrogen. All materials were characterized by X-ray diffraction crystallography, transmission electron microscopy, photon correlation spectroscopy, and Doppler electrophoretic light scatter analysis.
X-ray diffraction samples were prepared by mounting the powder on a glass slide using double-sized Scotch tape. CuKa radiation was used on a Norelco diffractometer. The spectrum obtained was compared with ASTM
standard data of tin oxide. (Powder Diffraction File, Card #21-1250. Joint Committee on Power Diffraction Standards, American Society for Testing and Materials, Philadelphia 1976.) The specimens for (TEM) were collected on a standard 3 mm diameter carbon coated copper mesh by dipping into a dispersion of the (UFP's) in 22-propanol. The samples were examined on a JEOL-STEM 100 CX at an acceleration voltage of 60-80 KV.
To create working dispersions of these metal oxides, 1.5 to 3.0 mg of metal oxide powder was added to 1.5 ml double distilled H20 in a dust-free screw top microcentrifuge tube (Sarsted) and vortexed for 30 sec-onds. The mixture was then sonicated for 16 to 24 hours followed by a second 30 seconds vortex. The submicron fraction was then isolated by pelleting macroparticulates by microcentrifugation 16,000 xg for 15 sec-onds. Approximately 1.3 ml of supernatant was then removed and placed in another dust-free screw top microcentrifuge tube. A sample was prepared for photon correlation spectroscopy (Coulter N4MD) and Doppler electrophoretic light scattering (Coulter delsa 440) analysis by removing 50 to 100 ~l of the dispersion and placing it in a polystyrene cuvette and diluting it to a final volume of 1.00 ml with ddH20. The stability of the dispersion was determined by sequential measurements over a 24-hour period and was found to be stable. The stability of the dispersion with respect to progressive salinity of the solvent (increasing conductivity) was similarly determined. The stability increased with progressive salinity of the solvent.
1.00 ml of the dispersion was combined and stirred with 8.00 ml of ddH20 and 1.00 ml of 29.2 mM cellobiose stock in a 15.0 ml capacity ultrafiltration stir cell (Spectra) which has been fitted with a pre-rinsed 5x105 molecular weight cutoff type F membrane (Spectra). The sample was then left to stir for 15 minutes. After stirring, the excess cellobiose was removed by flushing through the cell chamber 250 ml of ddH20 by the action of a peristaltic pump at a rate that does not exceed 10.0 ml/min.
After washing, the filtrate was concentrated by the means of pressurized N2 gas to approximately 1.0 ml. Character was established by the removal of 500 ul of the treated dispersion by N4MD analysis. The mean disper-sion diameter was re-established at this step. The stability of the coated dispersion was determined by sequential measurements over a 24-hour period. The stability of the coated dispersion with respect to progressive salinity of the solvent (increasing conductivity) was similarly determined.
The resulting coated nanocrystalline particles are suitable for attachment of various proteins, peptides and pharmaceutical agents.

Example 4. PreDaration of Tricalcium Phosphate (TCP) Nanocrystalline Particles Coated With P5P:
1. Using two 60 cc syringes and a T-Luer lock, inject 50 mls of 0.75 m CaCI2 and 50 mls of 0.25 m Na2HP04 into a 120 ml pharmaceutical bottle in the cup sonicator. Sonicate for 30 minutes at room temperature to form suspension of TCP particles.

2. Spin the TCP preparation down in the centrifuge using the bucket rotor at 3000 rpm for 15 minutes to remove unreacted components.

-WO 95112392 PC'r/US94/12515 ~
2 ~ 4 _34 3. Resuspend the TCP particles in 50 mls of HPLC grade water and mix well. Spin down at 3000 rpm for 15 minutes. Repeat step 3, three times (3x).

4. Add 1.0 ml of 100 mg/ml pyridoxal-5-phosphate (P5P) and incubate for 30 minutes on a rocker arm at room temperature.

5. Lyophilize overnight.

6. Resuspend the P5P-TCP preparation in 50 mls of 0.1 n sodium hydroxide. Mix well. Spin down at 3000 rpm for 20 minutes. This removes the excess P5P. (This step may not have to be completed with all carbohydrates. Centrifugation and subsequent washing steps may be adequate . ) 7. Resuspend in 50 mls of PBS and spin down at 3000 rpm for 15 minutes. Repeat step 8 three times (3x). This removes the sodium hydroxide.

8. Resuspend pellet in 50 mls of HPLC grade water and spin down at 3000 rpm for 15 minutes. Repeat step 9 three times (3x). This removes the PBS.

9. Resuspend the pellet in 4.0 mls of HPLC water and 1.0 ml of 100 mM of sodium citrate to pH 7.2.

10. Sonicate for 15 minutes at room temperature to form suspension of particles which is ready for attachment of biochemically active agent.

~ WO 95112392 PCT/US9~/1251S
21 7~

Example 5. Preparation of TCP Nanocrystalline Particles Coated with Cellobiose:
The same procedure as described in Example 1 is followed except that cellobiose is substituted for P5P. The cellobiose coating is applied to the particles by suspending the particles in a stock solution of cellobiose.
The cellobiose stock solution is a 292 mM solution made by dissolving 1.000 gram of cellobiose in 9.00 mls of ddH20. Solution is accomplished at approximately 70C in orderto promote quick dissolution. The resulting cellobiose solution is filter sterilized through a rinsed 0.45 micron filter with the final volume being adjusted to 10.00 ml.
Sufficient cellobiose stock solution is added to 150 microliters of the ultrafine biodegradable particle dispersion so that the final concentration of the particle is 1.00 percent (w/v) or 29.2 mM. A typical volume for prepa-ration is 2.0 mls which is mixed four or five times by the action of a micro-pipetor. After mixing, the dispersion is allowed to equilibrate for two hours. Demonstra~ion of successful coating of the particles is provided by measuring the mobility of the particles (coated and uncoated) on a Coulter DELSA 440 doppler energy light scatter analyzer. The coated particles exhibit a relatively low mobility compared to the non-coated particles.
Measurements are also taken at various dilute salt concentrations to ensure that the observations with respect to mobility are not artifactual.
The coated particles are then used to attach antigenic proteins, peptides or pharmacological agents to prepare bioreactive particles.

Examcle 6. PreDaration of Nanoparticles with Cellobiose or P5P
Coatings: The tin oxide, ruthenium oxide and silicon dioxide nanoparticles prepared in Example 1-3 are coated with cellobiose or P5P in the same manner as TCP.

W095/12392 PCT/US94/12515 ~

~ 4~ ~4 Exampie 7. Pre~aring Meticulouslv Clean Biodegradable Nanocarticles:
1. Prepare 6 clean sonication tubes with 500 mg of biodegradable particles per tube.

2. In fume hood, fill tubes with HCI (10 N) approx. 8 ml/tube.

3. Sonicate for 30 min. (full power 1175 watts]/25C); three tubes per sonication treatment.
4. Centrifuge 30 min. at 2000 rpm.

5. Decant the acidic supernatant (in the fume hood), fill the tubes with HPLC grade water and then vortex.
6. Sonicate for 30 min [above conditions] and centrifuge for 30 [centrifuging is complete if the supernatant is clear].

7. Decant the supernatant, and fill the tubes with HPLC grade water and vortex.

8. Repeat steps 7 and 8 two more times.

9. Decant the preparation into a clean glass [pyrex] baking dish.
10. Anneal at 210C overnight.

11. Remove the dried biodegradable crystals by gentle scraping with a clean unpainted spatula and transfer into 6 clean glass sonicating tubes.

~ WO 95112392 PCTIUS94/12515 ~ ~2~ 37 12. Repeat steps 3 through 8.

13. Prepare a 10 kD (NMWL) 150 ml ultrafiltration cell, empty the contents only one[no more than 500 mg per filtration run] of the tubes into the cell, and wash 500 ml of HPLC grade water through the cell under a N2 pressure head of 20 psi (regulator pressure gauge reading).

14. After washing, adjust the preparation volume to 100.0 ml by using the appropriate volume markings on the side of the cell.

15. Take a concentration measurement by removing 1.0 ml of the prepa-ration from the cell and Iyophilizing it down in a pre-weighed 1.7 ml Eppendorf tube. After Iyophilization, take a mass measurement of the tube with its contents and subtract it away from the mass of the empty tube. This provides the initial density of the preparation.
Preferably, the concentration or density of the particles in the solution is about 10 mg/ml. If the initial density is lower than 10 mg/ml, then the solution should be further concentrated in the ultrafiltration cell.

Examole 8. Coating MeticulouslY Clean Biodegradable Nanor articles with a Molecular Stabilizing Film (Cellobiose):

Incubation/Lyo~hilization.
1. Sonicate the meticulously clean biodegradable particles (aqueous dispersion) prepared in Example 7 for 30 minutes at 25C at full power ~175 Watts].

2. Then as quickly as possible, exchange suspending medium from water (stock) to a solution of 500 mM cellobiose using either a bench top microcentrifuge (30 seconds, full speed of 14,000 RPM) for small volumes or for larger volumes a floor models centrifuge (model 21 K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes). Suspend the pelleted particles with 500 mM cello-biose, sonicate to aid dispersion (approximately 5 minutes at 25C
at full power [175 Watts]) and finally set the mixture on a rocking plate overnight in a cold room [4C].
3. The next day portion out the mixture into appropriately sized vessels for overnight Iyophilization.

4. Leave the tubes capped with a layer of parafilm around the cap and place them in a freezer until the washing step.

5. Reconstitute the particle/cellobiose in a suitable buffer depending on the application. Suitable buffers are low ionic strength buffered phosphate (PRB), water, or bicarbonate. Reconstitution in the buffer is accomplished by vortexing and a 5 minute sonication [175 Watts/
25C].

6. Wash by repeated centrifugation (using either a bench top micro-centrifuge [30 seconds, full speed of 14,000 RPM] for small volumes or for large volumes a floor model centrifuge [model 21 K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes]) and resuspension into the buffer.

~ W095/12392 ~2~ PCl IS94/12515 7. Take a concentration measurement by removing 1 ml of the suspen-sion dehydrating it in a Iyophilizer in a pre-weighed 1.7 ml Eppendorf tube, and massing.

8. Calculate the final volume necessary to bring the concentration to 1 mg/ml. Add enough buffer to bring the concentration of the particle/cellobiose preparation to 1 mg/ml.

Example 9. Preparing Meticulouslv Clean Particles of Brushite:
Reagents. 0.75 M CaC12: 55.139 CaCI2.2H2O is dissolved with HPLC grade water to 0.500L in a volumetric flask. Filter sterilize with 0.2 um sterile filtration unit and place in a sterile 500 ml culture medium flask.
Store at room temperature.
0.25 M Na2HPO4: 17.759 of anhydrous Na2HPO4 is dissolved with HPLC grade water to 0.500 L in a volumetric flask. Filter sterilize with 0.2 um sterile filtration unit and place in a sterile 500 ml culture medium flask.
Also store at room temperature.

Brushite synthesis. About a half hour before synthesis, prepare the sonicator by cooling down the cup horn. This is accomplished by adjusting the low temperature thermostat on the water condenser to 4C and dialing a setting of "4" on the peristatic circulator. Once the 4C mark is reached, prepare 50.0 ml of 0.75 M CaC12 and 50.0 ml of 0.25 M
Na2H2PO4 and load into 50 ml syringes. The syringes are then to be connected to a 3-way luer lock connector so that they are set in diametric opposition allowing the remaining luer port to be free to dispel product. Once the mixing apparatus is set up, place a sterile 120 ml sonicating flask in the cup horn and slowly power up the sonicator to 100% power. Position the mixing apparatus so that the free luer port is W095112392 2~7 ~%~ PCTIUS94/12515 ~

over the sonicating flask. Expel syringe contents into the flask as rapidly and evenly as possible so as to empty each syringe roughly at the same time. Then quickly secure a polypropylene liner over the sonicating flask and let sonicate for an additional 15 minutes.

Brushite washing. Roughly divide the preparation into two 50 ml blue top polypropylene tubes and pellet at 2000 rpm for 10 minutes (room temperature). Reconstitute by vortexing each pellet with sterile HPLC
grade water to 50 ml (or tube capacity) and pellet at 2000 rpm for 10 minutes. Repeat this wash 3 more times and reconstitute the last pellets to 50.0 ml. Transfer the dispersion to a sterile 120 ml sonicating flask with polypropylene liner. Place the flask in a previously cooled sonicator cup horn at 1 C. Sonicate at 100% power for 60 minutes.

ExamDle 10. Coating Meticulouslv Clean Particles of Brushite with a Molecular Stabilizing Film of Pyridoxvl-5-Pvronhosphate:

Brushite/Pyroxidal 5 phosphate (vitamine B6). Pellet 100 ml of the dispersion prepared in Example 10 so that the entire contents can be transferred to a 50 ml conical tube. Adjust the tube volume to 40.0 ml.
Then transfer the contents in 10 ml aliquots to four 15 ml conical tubes.
Dissolve 1000 mg of Pyroxidal-5-phosphate with 800 IJI of 10 N NaOH and adjust with water to 10 mls. Filter sterilize this clear yellow solution with a .2 I~m acrodisc and add 2.5 ml aliquots to each of the previously pre-pared 4 brushite tubes. Vortex each tube a few seconds to make certain that the contents are well dispersed. Lyophilize overnight [approx. 16 hrs]
at the low drying rate setting. The next morning resuspend in 50 ml aliquots of sterile HPLC grade water five more times. Pellet once more and transfer the pellets to four 15 ml conical tubes and adjust the final prepa-ration volume with water to 40.0 ml.

WO 95112392 2~? ~q PCT/US94/1251S

Example 11. Coating Meticulously Clean Particles of Brushite With a Molecular Stabilizinq Film of Citrate:

Brushite/citrate. Pellet the 100 ml of the dispersion prepared in Example 13 so that entire contents can be transferred to a 50 ml conical tube. Adjust the tube volume to 40.0 ml. Then transfer the contents in 10 ml aliquots to four 15 ml conical tubes. Add 10 ml of 100 mM citrate to each of the 15 ml conicals and nutate for 30 minutes at room temperature. Lyophilize overnight [approx. 16 hrs] at the low drying rate setting. The next morning resuspend in 50 ml aliquots of sterile HPLC
grade water five more times. Pellet once more and transfer the pellets to four 15 ml conical tubes and adjust the final preparation volume with water to 40.0 ml.

ExamDle 12. PreDaration, isolation and surface adsorption of human serum transferrin proteins: Nanocrystalline tin oxide was synthesized by D.C. reactive Magnetron sputtering (inverted cathode). A 3" diameter target of high purity tin was sputtered in a high pressure gas mixture of argon and oxygen. The ultra-fine particles formed in the gas phase were collected on coppertubes cooled to 77K with flowing liquid nitrogen. All materials were characterized by x-ray diKraction crystallography, selected area electron diffraction, transmission electron microscopy, photon correlation spectroscopy, and energy dispersive x-ray spectroscopy. X-ray diffraction samples were prepared by mounting the powder on a glass slide using double-sized Scotch tape. CuK(alpha) radiation was used on a Norelco diffractometer. The spectrum obtained was compared with ASTM
standard data of tin oxide. The specimens for transmission electron microscopy and selected area diKraction were collected on a standard 3 mm diameter carbon coated copper mesh by dipping into a dispersion of the nanocrystalline materials in 2-propanol. The samples were examined W095/12392 PCT/US9~112515 ~

4~ -42-on a JEOL-STEM 100 CX at an acceleration voltage of 60-80 KeV. The 2-propanol suspension of particles was also characterized by photon correlation spectroscopy at 22.5C, 600 s run time on a Coulter N4MD.
Energy dispersive x-ray spectroscopy was performed on a JEOL JSM-T330A scanning electron microscope using Kevex quantex V software.
To create working dispersions of these metal oxides for the synthesis of compositions in accordance with the present invention, 0.5 mg of rnetal oxide powder was added to 1.0 ml of a 29.2 mM cellobiose-phosphate buffered saline solution in a dust free screw top glass vial and sonicated for 20 minutes at 22.5-35C. The submicron fraction was then isolated by pelleting macroparticulates by microcentrifugation at 16,000xg for 30 seconds. Approximately 900 ~l of supernatant was then removed and placed in a dust free screw top microcentrifuge tube. An aliquot was removed for photon correlation spectroscopy (Coulter 1\14MD) and Doppler electrophoretic light scattering (Coulter DELSA 440) analysis. Aliquots were also removed for characterizing the stability of the coated dispersion over time and with respect to progressive salinity of the solvent (increasing conductivity) .
To adsorb protein to the cellobiose coated metal oxide nanocrystal-line cores, the core sample was diluted to 10.0 ml with Ca++ and Mg++
free phosphate buffered saline (Gibco). Forty (40.0) /19 of purified human serum transferrin (4/Jg//~l) (Gibco), whose antigenicity was verified by ELISA, was then added to a 10 ml stir cell (Spectra). The sample was then left to stir slowly for 30 minutes, taking great care not to allow foaming.
After the addition period, 15 ml of Ca++ and Mg++ free phosphate buf-fered saline (Gibco) was then washed through the cell under a 2 psi nitro-gen gas pressure head. After washing, the sample was again concentrated to 1.00 ml under N2 and a 500 IJI sample was removed for analysis by photon correlation spectroscopy, Doppler electrophoretic light scatter and transmission electron microscopy as detailed below.

W0 95/12392 2~ , PCT/US94/12515 Conformational integrity was assessed by measuring the retained antigenicity of the bound protein. To the sample cell, 50.0 111 of rabbit polyclonal anti-human transferrin antibody (Dako), whose antigenicity was confirmed by ELISA, was added to the concentrated 1.0 ml reaction pro-duct at 37.5C with gentle stirring. After a 30 minute incubation period, 15 ml of Ca++ and Mg++ free phosphate buffered saline (Gibco) was then washed through the cell under a 2 psi nitrogen gas pressure head and the reaction volume was again reduced to 1.0 ml.
A 200 /~1 aliquot of blocking agent, 1% w/v bovine serum albumin in divalent free saline, was added followed by a 10 minute equilibration period. The secondary antibody, 30 nm gold conjugated goat anti-rabbit polyclonal IgG (Zymed), was then added and the reaction mixture was allowed to incubate for 30 minutes. A sample was removed, chopped on a transmission electron microscopy grid, and vacuum dried. The mixture was again washed with 15 ml of divalent free saline under a nitrogen pressure head and then fixed with glutaraldehyde. One ml of 3% solid bovine collagen (Collagen Corp.) was then added to the mixtures and the composite was ultracentrifuged at 10Gxg for 30 minutes yielding a pellet that was then routinely processed as a biological specimen for transmission electron microscopy. Ten nm thick sections were viewed on a Zeiss trans-mission electron microscopy. Control samples were prepared as above without the cellobiose intermediate bonding layer.
Transmission electron micrographs showed that the D.C. magnetron sputtered tin oxide was composed of individual particles measuring 20-25 nm in diameter which aggregated into clusters measuring 80 to 120 nm in diameter. By photon correlation spectroscopy, these same particles when dispersed in distilled water produced agglomerates measuring 154 i 55 nm. The tin oxide particles were fully crystalline as characterized by electron and x-ray diffraction. Energy dispersive x-ray spectroscopy showed no other elements present as impurities.

W0 95112392 2 ~ ~ PCT/US94/12515 By Doppler electrophoretic light scatter analysis, tin oxide exhibited a mean mobility of 2.177 i 0.215 llm-cm/V-s in aqueous solutions rang-ing from 10.8 to 20.3 I~M NaCI. Following cellobiose surface coating in a 1% solution, tin oxide exhibited a mean mobility of 1.544 i 0.241 ~m-cm/V-s in aqueous solutions ranging from 0.0 to 21.0 IIM NaCI. The oxide agglomerated in salt concentrations of greater than 40.0 ,uM and in solu-tions of increasing cellobiose concentration.
Following transferring binding, the crude tin oxide/cellobiose/protein conjugates measured 350 i 84 nm by photon correlation spectroscopy and transmission electron microscopy. Vacuum dried dropped samples with low concentration gold antibody measured 35-50 nm. Without the cellobiose bonding layer, vacuum dried sections measured 400 to > 1000 nm. Occasional antibody bonding was noted. Following high concentra-tion immunogold labeling and filtering, the thin section cellobiose treated specimens measured 50-100 nm. Positive gold binding was identified in approximately 20% of the appropriately coated samples whereas negative controls (prepared as above but lacking the primary rabbit antibody) exhibited approximately 1% nonspecific binding.
As can be seen from the above examples, the biological activity of protein absorbed to the surface of carbohydrate-treated nanocrystalline metal oxide particles is preserved.

Example 13. Preparation and Characterization of Epstein-Barr Virus Decoys: Nanocrystalline tin oxide particles were synthesized by D.C.
reactive Magnetron sputtering as previously described in Example 1.
Elutriated sucrose gradient purified Epstein-Barr virus (EBV) acquired from the B95-8 cell line were purchased from Advanced Biotechnologies, Inc., Columbia MD. Each viral aliquot contained approximately 5.00 x 101 virus particles/ml suspended in 10mM TRlS-150mM NaCI ph 7.5 buffer (approximately 0.94 mg/ml protein). The virions were solubilized 0.75%

WO 95tl2392 PCT/US9411251S
2~ 7~

(v/v) Triton X100 and then ultracentrifuged at 150,000xg for 60 minutes to pellet the DNA core using a modification of the method described by Wells. (Wells A, Koide N, Klein G: Two large virion envelope glycoproteins mediate EBV binding to receptor-positive cells. J Virology 1982;
41 :286-297.) Following dialysis, the supernatant EBV extract was charac-terized by both SDS-PAGE (denatured) rBiorad Mini Gel ll, 4-20% gradient gel, 200V x 45 minutes and stained with silver] and size exclusion HPLC
(non-denatured) lWaters 620 system with a WISP autoinjector and 7' photodiode array detector, 0.5 ml/minute over a Waters SW300 GFC
column using a 100mM NaCI/20mM TRIS pH 9.4 gradient mobile phase].
Control (non-EBV) proteins were extracted from aliquots of Lambda phage virus ~Pharmacia, Milwaukee Wl] using the same methods as described above.
Aliquots of the tin oxide powder weighing approximately 1.5 mg were initially suspended in 3.0 ml of 29.2 mM cellobiose solution in a dust free glass vial by liberal vortexing [Vortex Genie, Scientific Industries, Bohemia, NY]. The resultant brownish cloudy suspension was then soni-fied at 175 W for 10.0 minutes at a frequency of approximately 20 kHz at 25 C [Branson 2" Cup Horn, Branson Ultrasonics Corp., Danbury CT]. The dispersion was clarified by microcentrifugation at 16,000xg for 15 sec-onds. The remaining pellet was then discarded in favor of the supernatant.
Unadsorbed cellobiose was removed by ultrafiltration against 20 mls of 25 mM phosphate reaction buffer (pH 7.40 25mM HP042-/H2P041-) in a 10 kD
nominal molecular weight filtered stir cell lPharmacia] under a 7.5 psi N2 gas head at 37.5C. Aliquots of the intermediate product were charac-terized by photon correlation spectroscopy and, following dialysis as described below, by doppler electrophoretic light scatter analysis.
The process of viral protein adsorption was initiated by the removal of the mild triton surfactant from 250 1~1 aliquots of EBV extract by ultrafiltration against 25 mls of phosphate reaction buffer at 4C in a 10 WO 95/12392 PCT/US94/12515 ~t 2~ 4~4 kD nominal molecular weight stir cell and then adjusted to a concentration of 1.0 /~g/~l or approximately 1.0 ml final volume. Then 500 ul of the triton free EBV extract was quickly added to a MD nominal molecular weight stir cell with 2.0 ml of the surface treated tin oxide dispersion prewarmed to 37.5C. The mixture was then slowly stirred while being incubated at 37.5C for 2.0 hours. After incubation the unabsorbed EBV
extract was removed by ultrafiltration against 25 mls of phosphate reaction buffer.
Control (non-EBV) decoys fabricated with lambda phage viral protein extracts were synthesized using the same process described above.
Intermediate components, the final assembled decoys, and whole Epstein-Barr virions were characterized by doppler electrophoretic light scatter analysis [DELSA 440, Coulter Electronics Inc., Hialeah, FLl to determine their electrophoretic mobility (surface charge) in a fluid phase.
Nine phosphate buffer solutions having at 25C pH's ranging between 4.59 and 9.06 and corresponding conductivities ranging between 2.290 and 4.720 mS/cm were prepared. Aliquots of raw tin oxide, surface modified cellobiose covered tin oxide, synthesized EBV decoy, and whole EBV were dialyzed against each of the nine solutions and the mobilities of the particulates in dispersion were then measured at field strengths of 4.0, 5.5, 5.5, and 8.0 mA respectively. The mobility values acquired simul-taneously by the 4 angled detectors of the instrument were averaged and the means of 3 measurements per dispersion were recorded.
The synthesized EBV decoys and control decoys were characterized by immunoagglutination photon correlation spectroscopy to determine the antibody reactivity of their surfaces. Positive reactivity was assessed by incubating the EBV decoy for 60 minutes at 37.5C with a cocktail of anti-EBV murine monoclonal antibodies (1 ug each of anti-EBV-VCA, anti-EBV EA-R, anti-EBV MA, and anti EBV EA-D) in 15% lactose, 0.9%
NaCI, 10 mM HEPES buffer, and 0.2% NaN3 [DuPont, Wilmington, DE]).

WO 95/12392 ~ PCT/US91/12515 47_ Background reactivity was assessed by incubating the EBV decoy with irrelevant murine IgG,. Specificity was assessed by reacting the lambda phage decoy with monoclonal anti-EBV murine antibodies. Agglutination was measured by photon correlation spectroscopy at a 90 angle [N4MD, Coulter].
~ntibody affinity intensity was assessed by immunogold transmis-sion electron microscopy using the particulates and antibodies listed above and then adding secondary anti-murine 30 nm gold-labeled antibodies (Faulk W, Taylor G. Immunocolloid method for electron microscopy, /mmunochemistry 8:1 081-1083, 1 971 ).
Labeling of the EBV decoy (positive reaction) was accomplished by incubating a 20~1 mixture of murine monoclonals (1 IJ9 anti-EBV-VCA and 1 /Jg anti-EBV EA-R in 15% lactose, 0.9% NaCI, 10 mM HEPES buffer, and 0.2% NaN3 [DuPont] ) with a fresh 0.5 ml sample of EBV decoy at 37.5C
for 30 minutes in a 300 kD nominal molecular weigh stir cell. Unbound antibody was then removed by ultrafiltration against 20 mls of phosphate reaction buffer under a 5.0 psi N2 pressure head. After washing, 50 ~l of goat anti-murine antibody covalently fused to 30 nm gold spheres (10 particles/mltZymed Laboratories, San Francisco, CA]) were incubated with 200 ~Is of the labeled particles in a 1 M nominal molecular weight stir cell at 37.5C for 30 minutes. Unbound secondary antibody was removed by ultrafiltration against 10 mls of phosphate reaction buffer.
Labeling of the EBV decoy (negative reaction) was accomplished by incubating 2.5~1 of murine polyclonal nonspecific IgG1 (1--~9//11 in 15 mM
NaCI pH 7.4 [Sigma Chemical Corp., St. Louis, M0]) with a fresh 0.5 ml sample of EBV decoy as described above followed by the same washing and gold-labeling steps. Labeling of the lambda phage control decoy (negative reaction) was accomplished by incubating a 20 /~1 mixture of murine monoclonal anti-EBV antibodies with the lambda phage virus coated decoy using the same procedure detailed above.

WO 95112392 PCT/US94112515 ~

Immunoiabeled particles were prepared for electron microscopy in two ways. A direct immersion technique where a carbon coated copper viewing grid [Ted Pella Inc., Redding, CA] was submersed into sample for approximately 5 seconds and then fixed in 5% glutaraldehyde for 1 minute, was used for all reactions as a fast screening technique. A more involved method adding glutaraldehyde directly to the reaction solution, then pelleting the product at 16,000xg for 5 minutes into 0.5 ml soft agar preparation (0.7% agarose [Sea Kem, Temecula, CA] in H20). Then the resultant agar plugs were embedded in plastic and sectioned into 0.1 ~m sheets for viewing.
Analysis of both the positive and negative controls was performed by examining pelleted samples of the labeled reaction products by transmission electron microscopy. The relative intensity of antibody binding was determined by counting the number of tin oxide based particles observed to have bound gold spheres (% positive) and then noting the number of gold spheres bound to a given particle (intensity, number/event) .
The ultrafine tin oxide particles measured 20-25 nm in diameter and formed aggregates measuring 80 to 120 nm in diameter by transmission electron microscopy. By photon correlation spectroscopy, these same particles when dispersed in distilled water produced ag~lomerates measur-ing 154 i 55 nm. The tin oxide particles were fully crystalline as characterized by electron and x-ray diffraction. Energy dispersive x-ray spectroscopy showed no other elements present as impurities.
Characterization of the EBV proteins by SDS-PAGE showed two distinct protein bands. The first, existing as a dimer suggesting variable glycosylation, exhibited a molecular weight of approximately 350 kd which is consistent with the predominant envelope glycoprotein of EBV. The second exhibited a molecular weight of approximately 67 kd consistent with serum albumin which apparently adsorbs avidly to the viral surface.

~ 49-HPLC confirmed the presence of two distinct bands that exhibited spectro-photometric absorption maxima at 280 nm consistent with proteins. The predominant peak had a chromatographic retention time of 10.30 minutes and could be suppressed 90% by monoclonal anti VCA. The second and relatively minor peak exhibited a chromatographic retention time of 15.75 minutes similar to bovine serum albumin standards.
The previously described Doppler electrophoretic mobility studies conducted between the pH range of 4.5 to 9.0 demonstrated 3 distinct patterns. First, both the decoy and native EB virus retained virtually iden-tical mobilities of approximately -1.4~m-cm/V-s throughoutthe pH range.
Second, untreated tin oxide exhibited a mobility of approximately -1.0 ~m-cm/V-s at a pH of 4.5 which then rose rapidly to -3.0 llm-cm/V-s at pH
values of 5.0 and higher. Third, surface modified tin oxide treated with cellobiose retained a mobility of approximately -1.5 llm-cm/V-s until it increased rapidly to -2.5 um-cm/V-s at a pH of 7.5.
The previously described photon correlation spectroscopy showed that native EBV measured approximately 102 +/-32 nm and the synthe-sized EBV decoy measured approximately 154 + /- 52 nm. Synthesized EBV decoy, when reacted with the monoclonal anti-EBV cocktail, aggluti-nated to form 1534 +/ -394 nm masses. Synthesized EBV decoy, when reacted with non-specific mouse IgG, only increased slightly in size with agglutination diameters of 230 +/ -76 nm. Lambda phage decoy, when reacted with the monoclonal anti-EBV cocktail, only increased slightly in size with agglutination diameters of 170 +/-35 nm.
The previously described transmission electron microscopy of anti-EBV antibody labeled EBV decoy particles revealed a positive gold staining frequency of 23.51 % +/ -5.53 with an average staining intensity of 7.41 gold labels per event. Examination of non-specific mouse IgG
antibody labeled EBV decoy particles revealed a positive gold staining frequency of 5.53% +/ -2.04 with an average staining intensity of 1.00 WO 95/12392 PCT/US94/1251S ~

gold labels per event. Examination of anti-EBV antibody labeled lambda phage decoy particles revealed a positive gold staining frequency of 7.21 %
+/-1.26 with an average staining intensity of 1.06 gold labels per event.

Example 14: In Vivo Elicitation of Antibodies By EDstein-Barr Virus Decoy: Four sensitization solutions were prepared and delivered once every other week by intramuscular injection in three 250 /11 aliquots to New Zealand rabbits aged approximately 8 weeks. The first four animals received approximately 109 whole EBV virions (approximately 32 ~9 of gp350 estimated by integration of the spectrophotometric absorption curve at 280 nm against a 25 /Jg bovine serum albumin standard) dispersed in phosphate reaction buffer per injection. The second four animals received 321~9 per injection of isolated and purified gp350 using the same injection protocol. The third group received EBV viral decoys (Example 5) synthe-sized from a starting aliquot of 32 ~9 of gp350 per injection. The last group received cellobiose coated in tin oxide dispersed in phosphate reaction buffer. Injections were free of adjuvant. Whole blood was removed using aseptic techniques via cardiac puncture 2 weeks following each of the three injections and the animals were terminated by cardiac puncture followed by lethal sedation at 6 weeks. Serum was extracted by microcentrifugation at 16 kg of whole blood for 1 minute and then stored frozen at -70C pending analysis.
Immunospecific antibody against whole EBV virions (ABI) was assayed by ELISA. Approximately 109 virions/ml in phosphate reaction buffer were diluted 1:10 in coating buffer and then allowed to adsorb overnight at 4C in polycarbonate assay plates (Falcon). Rabbit serum affi-nity for the bound EBV virions was determined by the colorimetric reaction of goat anti-rabbit IgG alkaline phosphatase (Sigma) developed with para-nitrophenyl phosphate. The concentration of immunospecific IgG
were determined by comparison to a calibration curve using nonspecific I7~2~ _51 -rabbit IgG as the adsorbed antigen and by subtracting the baseline values recorded from the wells containing serum from the rabbits stimulated with tin oxide only.
Serum collected from the 4 rabbits sensitized with tin oxide showed no increased anti-EBV activity over pre-immune serum at any of the three two week sampling intervals. The remaining 3 groups showed a progress-ive rise in the concentration of anti-EBV specific IgG over the 6 week period. Animals sensitized with purified EBV proteins alone showed a maximum of approximately 0.05 ug/~l anti-EBV IgG at six weeks. In contrast, animals sensitized with either whole EBV or decoy EBV exhibited a statistically significant four fold greater response with approximately 0.20 /~g/ul of anti-EBV IgG at six weeks. The immunospecific responses to decoy EBV and whole EBV were virtually identical.
As is apparent from Examples 5 and 6, the synthesized EBV decoy in accordance with the present invention possesses the same surface charge as native virus, is recognized specifically and avidly by monoclonal antibodies, and evokes immunospecific antibodies with the same effective-ness as whole virus. Using photon correlation spectroscopy, the number of particles that agglutinated in the three reaction conditions were calculated from the measured diameters of the aggregates. These calcu-lations indicate that monoclonal anti-EBV antibodies produce agglutinated masses consisting of an average 988.0 decoy EBV particles. Non-specific mouse IgG antibodies produce agglutinated masses consisting of an average 3.33 decoy EBV particles, while monoclonal anti-EBV antibodies produce agglutinated masses consisting of an average 1.35 decoy control - lambda phage particles. These measured results show that the measuredagglutination potential of the EBV decoy in accordance with the present invention is almost three orders of magnitude greater than controls. The immunogold transmission electron microscopy shows that the gold labeled antibody staining of anti-EBV labeled EBV decoys is 25 to 30 times greater than controls. The ELISA analysis of the immunospecificity of anti-EBV
IgG elicited in the rabbits by the EBV decoy is similar to the response eli-cited by native virus and is 4 fold greater than the response elicited by isolated purified proteins. Examples 5 and 6 are summarized in Kossovsky, N. et al., Nanocrystalline Epstein-Barr Virus Decoys, Journal of Applied Biomaterial, Vol. 2, 251-259, (1991).

Exam~le 15. Preoaration of HIV Deco~Js: The following procedure was used to adsorb HIV membrane antigens onto diamond nanocrystalline particles to provide HIV decoys.

HIV Workup. 1.0 ml of HIV (TCID 50 titre which varied between 10575 to 107-17 as determined by the producer Advanced Biotechnology, Inc.) was dialyzed into PBS by 100 KD ultrafiltration and frozen down to -70C until needed. On injection day the viral stock was thawed on ice and diluted to 1:25 in PBS. 100 ul of this preparation was used for injec-tion. 1.0 ml of HIV (10575 transforming units per ml) [ABI] was added to 0.5 ml of envelope extraction buffer [1.0% of Triton X 100\0.25 mM DTT\
10 mM Tris pH 7.4\1.0 mM MgCI] and was allowed to incubate for 1.0 hr at room temp. The extract was then ultracentrifuged at 100K*g for 2.0 hrs [35krpm SW50.1 Beckman rotor] at 4.0C to remove nucleocapsid.
Removal of Triton X and envelope protein enrichment was accomplished by incubation with a 300 ul slurry of polystyrene micro beads [Spectra Gel D2] and subsequent 100 kD ultra filtration into PBS. For a 100 ul injection the extract volume was corrected to a 1.0 ml volume and diluted 1 :25 in PBS or to a protein concentration of around 2.5 ug/100ul/injection volume.
Protein quantization was conducted by HPLC. HPLC conditions were as follows: Waters GFC SW300/Mobile phase:300mM NaCI, 20mM phos-phates pH 7.4/ one major peak with a retention time of around 8.9 minutes ~ 53_ at a flow rate of 0.5 ml per min/integration was done against BSA
standards.

Preparation of HIV Decoy. HIV extract was adjusted to 1.0 ml volume after being ultrafiltered against pH 7.40 20 mM phosphate buffer and was incubated with 1.0 ml of diamond particles which had been coated with 500 mM cellobiose at 4.0C for 24 hours. The diamond parti-cles had an average particle size on the order of 50 nm. After adsorption the decoy dispersion was prepared for injection by 300 kD ultrafiltration against PBS to remove unadsorbed protein and was adjusted to 1.0 ml with PBS and parceled out for ten 100 111 injections.

Immunological Activity of HIV Decov. Rabbits, guinea pigs, and mice were injected with either live virus, protein extract, protein extract mixed with Freund's adjuvant, or the HIV decoy virus. Antibody titres against whole virus were measured by ELISA and characterized by western blotting. Cell mediated reactivity was assessed in the guinea pigs by dermal skin challenge with live virus follows by biopsy.
At physiological pH, the mean electrophoretic mobility and average dispersion diameter (50 nm) of these synthetic carriers closely mimicked that of their infectious counterparts. Vaccination of mouse, guinea pig, and rabbit with the HIV decoy elicited the production of antisera which exhibited specific binding to whole HIV preparation as measured by ELISA.
The histological analysis of earprick sites for animal sensitized to decoy virus and whole virus showed similar (qualitative and quantitative) reac-tions which differed significantly from both Freund's-sensitized animals and purified protein-sensitized animals at 1, 2, 7 and 24 weeks. Binding specificity was confirmed by Western blots.
As shown in the above example, the HIV decoy of the present inven-tion has a number of characteristics which are shared with native whole HIV virus. These characteristics include: size, surface charge, immuno-recognition, ability to eiicit comparable antibody titers, and the magnitude and character of cellular response. These attributes show that the decoy virus in accordance with the present invention can function effectively as a vaccinating agent.
Methods of obtaining meticulously clean solid surfaces, either by cleaning pre-formed solids or by generating clean solids and surfaces de novo; methods for applying solutions containing carbohydrates, carbohy-drate derivatives, and other macromolecules with carbohydrate-like components characterized by the abundance of -OH (hydroxyl) side groups;
methods for Iyophilization to yield molecular stabilizing surface films; and methods for immobilizing (a) member(s) of a BRP are described in the additional examples below.

Example 16. Preoaring a Meticulously Clean Carbon Ceramic (Diamond) Nanoparticles:

1. Prepare 6 clean sonication tubes with 500 mg of particles per tube.

2. In fume hood, fill tubes with HCI (10 N) approx. 8 ml/tube.

3. Sonicate for 30 min. (full power [175 watts]/25C); three tubes per sonication treatment.

4. Centrifuge 30 min. at 2000 rpm.

5. Decant the acidic supernatant (in the fume hood), fill the tubes with HPLC grade water and then vortex.

WO 95/12392 ~? PCT/US94tl2S15 1~, 6. Sonicate for 30 min labove conditions] and centrifuge for 30 [centrifuging is complete if the supernatant is clear].

7. Decant the supernatant, and fill the tubes with HPLC grade water and vortex.

8. Repeat steps 7 and 8 two more times.

9. Decant the preparation into a clean glass ~pyrex] baking dish.
10. Anneal at 210C overnight.

11. Remove the dried diamond crystals by gentle scraping with a clean unpainted spatula and transfer into 6 clean glass sonicating tubes.
12. Repeat steps 3 through 8.

13. Prepare a 10 kD (NMWL) 150 ml ultrafiltration cell, empty the contents only one[no more than 500 mg per filtration run] of the tubes into the cell, and wash 500 ml of HPLC grade water through the cell under a N2 pressure head of 20 psi (regulator pressure gauge reading) .

14. After washing, adjust the preparation volume to 100.0 ml by using the appropriate volume markings on the side of the cell.
.

15. Take a concentration measurement by removing 1.0 ml of the prepa-ration from the cell and Iyophilking it down in a pre-weighed 1.7 ml Eppendorf tube. After Iyophilization, take a mass measurement of the tube with its contents and subtract it away from the mass of the empty tube. This provides the initial density of the preparation.
Preferably, the concèntration or density of the particles in the solution is about 10 mg/ml. If the initial density is lower than 10 mg/ml, then the solution should be further concentrated in the ultrafiltration cell.

Example 17. Coating Meticulouslv Clean Diamond Nanoparticles with a Molecular Stabilizing Film (Cellobiose):

Incubation/Lvor hilization.
1. Sonicate the meticulously clean carbon (diamond) (aqueous disper-sion) prepared in Example 16 for 30 minutes at 25C at full power [175 Watts].

2. Then as quickly as possible, exchange suspending medium from water (stock) to a solution of 500 mM cellobiose using either a bench top microcentrifuge (30 seconds, full speed of 14,000 RPM) for small volumes or for larger volumes a floor models centrifuge (model 21 K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes). Suspend the pelleted carbon with 500 mM cello-biose, sonicate to aid dispersion (approximately 5 minutes at 25C
at full power [175 Watts]) and finally set the mixture on a rocking plate overnight in a cold room [4C].

3. The next day portion out the mixture into appropriately sized vessels for overnight Iyophilization.

4. Leave the tubes capped with a layer of parafilm around the cap and place them in a freezer until the washing step.

WO 95/12392 ~ PCT/US94/12515 5. Reconstitute the carbon/cellobiose in a suitable buffer depending on the application. Suitable buffers are low ionic strength buffered phosphate (PRB), water, or bicarbonate. Reconstitution in the buffer is accomplished by vortexing and a 5 minute sonication [175 Watts/
25C].

6. Wash by repeated centrifugation (using either a bench top micro-centrifuge [30 seconds, full speed of 14,000 RPM] for small volumes or for large volumes a floor model centrifuge ~model 21 K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes]) and resuspension into the buffer.

7. Take a concentration measurement by removing 1 ml of the suspen-sion dehydrating it in a Iyophilizer in a pre-weighed 1.7 ml Eppendorf tube, and massing.

8. Calculate the final volume necessary to bring the concentration to 1 mg/ml. Add enough buffer to bring the concentration of the carbon/cellobiose preparation to 1 mg/ml.
Examcle 18. Prenaring Meticulouslv Clean Carbon Particles:

1. 2 grams of GE carbon powder was mixed with 25 ml 30% hydrogen peroxide + 7~; ml 36N sulfuric acid in a 250 ml Belco stir flask ~designed for suspension cultures). The reaction is exothermic and produces caustic vapors. Therefore it is advised to follow these precautions: 1. work inside a fume hood; 2. do not completely seal the Belco jar screw tops allowing ventilation through the 2 arms of the jar. Stir moderately for 8 days.

2. Pour the solution into 2 x 50 ml centrifuge tubes (approximately 40 ml each). Discard the last 15-20% of the solution, saving only the whiter material. Spin the carbon to a pellet using 8,000 RPM for 1 minute (room temperature). Suspend pellet with 20 mM phosphate buffer (7.4). Wash 3 times. At the third washing step, the centrifu-gation period may need to be extended to 5-10 minutes since carbon is less precipitous with increasing pH. After the final wash, suspend pellet into HPLC water and store at room temperature. The resulting particles had a mean size of 260 nm +/- 87 nm.
Results: Mean Size 260 nm +/- 87 nm; STD analysis 282 nm (98%) and 35.2 nm (2%) dust (4%) Example 1~. Immobilizing a Member of a Biochemically Reactive Pair (BRP) to a Coated Meticulouslv Clean Solid Surface:

1. One ml of Epstein-Barr virus EBV [ABI] was added to 4.0 ml of envelope extraction buffer [1.0% of Triton X 100\0.25 mM
dithiothreitol\10 mM Tris pH 7.4\1.0 mM MgCI] and was allowed to incubate 1 hour at room temperature. The extract was then ultra-centrifuged at 100K*g for 2.0 hrs [35 krpm SW50.1 Beckman rotor]
at 4C to remove nucleocapsid.

2. Pellet is discarded in favor of supernatant.
3. The supernatant is transferred to a 100 kD ultrafiltration unit, kept cold with circulating water at 4C.

4. Begin continuous dialysis using a total of 200 ml fresh, sterile PRB
(20 mM phosphate, pH 7.4). For a 100 ul injection the extract volume was corrected to a 1.0 ml volume and, immediately prior to injection, is diluted 1:25 in PBS or to a protein concentration of around 2.5 ug/100 ul/injection volume. Protein quantification can be done by HPLC [HPLC conditions are as follows: Waters GFC
SW300/Mobile phase: 300 mM NaCI, 20 mM phosphates pH 7.4/
one major peak with a retention time of around 8.9 minutes at a flow rate of 0.~ ml per min./lntegration was done against BSA
standards] .

5. After transferring the EBV extract to the 100 kd filter unit, add the carbon/cellobiose particles prepared in Example 10 to a final concen-tration of 1 mg/ml. Begin continuous dialysis using a total of 200 ml fresh, sterile PRB (20 mM phosphate, pH 7.4J. If the bound HBV
is being prepared for injection, adjust to a final volume of 1.0 ml and dilute to 1:25 with PBS for a 100 ul injection. For all other uses, the bound HBV is stored at 4C in PRB.

Example 20. Immobilizin~ a Member of Biochemically Reactive Pair (BRP) to a Goated Meticulously Clean Solid Surface:
1. Murine Lymphotropic virus (MuLV) extraction: MuLV stock [ABI]
diluted 1:5 (e.g. 1 ml stock virus diluted to a final volume of 5 ml with the dilutant) with Triton X-100 extraction buffer [1.0% of Triton X 100\0.25 mM dithiotreitol\10 mM Tris pH 7.4\1.0 mM
MgCI] and was allowed to incubate overnight at 4C. The extract was then ultracentrifuged at 1 OOK*g for 2.0 hours [35k rpm SW50.1 Beckman rotor] at 4C to remove the nucleocapsid.

2. Pellet is discarded in favor of supernatant.

WO 95/12392 PCTIUS9~/12515 3. MuLV decoy synthesis: It is desirable to use aseptic technique throughout the synthesis. Setup the stir cell unit such that access to the reaction mixture is rigorously controlled. Transfer the MuLV
extract to a 100kd filter unit of 10 ml volume and add the carbon/
cellobiose cores to a final concentration of 1 mg/ml. Begin continu-ous dialysis using a total of 200 ml fresh, sterile PRB (20 mM
phosphate, pH 7.4). If the decoy is being prepared for injection, adjust to a final volume of 1.0 ml and dilute to 1:25 with PBS for a 100 ul injection. For all other uses, the decoy is stored at 4C in 1 0 PRB.

Example 21. PreParing a Meticulously Clean Solid Surface of Calcium Phos~hate Dihydrate (Brushite):

Reagents. 0.75 M CaC12: 55.139 CaCI2.2H2O is dissolved with HPLC grade water to 0.500L in a volumetric flask. Filter sterilize with 0.2 um sterile filtration unit and place in a sterile 500 ml culture medium flask.
Store at room temperature.
0.25 M Na2HPO4: 1 7.75g of anhydrous Na2HPO4 is dissolved with HPLC grade water to 0.500 L in a volumetric flask. Filter sterilize with 0.2 um sterile filtration unit and place in a sterile 500 ml culture medium flask.
Also store at room temperature.

Brushite synthesis. About a half hour before synthesis, prepare the sonicator by cooling down the cup horn. This is accomplished by adjusting the low temperature thermostat on the water condenser to ~C and dialing a setting of "4" on the peristatic circulator. Once the 4C mark is reached, prepare 50.0 ml of 0.75 M CaC12 and 50.0 ml of 0.25 M
Na2H2PO4 and load into 50 ml syringes. The syringes are then to be connected to a 3-way luer lock connector so that they are set in diametric WO 95/12392 ,~ PCT/US94112515 opposition--allowing the remaining luer port to be free to dispel product.
Once the mixing apparatus is set up, place a sterile 120 ml sonicating flask in the cup horn and slowly power up the sonicator to 100% power. Posi-tion the mixing apparatus so that the free luer port is over the sonicating flask. Expel syringe contents into the flask as rapidly and evenly as possi-ble so as to empty each syringe roughly at the same time. Then quickly secure a polypropylene liner over the sonicating flask and let sonicate for an additional 15 minutes.

Brushite washing. Roughly divide the prep into two 50 ml blue top polypropylene tubes and pellet at 2000 rpm for 10 minutes (room tempera-ture). Reconstitute by vortexing each pellet with sterile HPLC grade water to 50 ml (or tube capacity) and pellet at 2000 rpm for 10 minutes. Repeat this wash 3 more times and reconstitute the last pellets to 50.0 ml. Trans-fer the dispersion to a sterile 120 ml sonicating flask with polypropylene liner. Place the flask in a previously cooled sonicator cup horn at 1 C.
Sonicate at 100% power for 60 minutes.

Example 22. Coating a Meticulously Clean Solid Surface of Calcium Phosphate Dihydrate (Brushite) with a Molecular Stabilizing Film of Pvridoxyl-5-Pyronhosphate:

Brushite/Pyroxidal 5 Dhosphate (vitamine B6). Pellet 100 ml of the dispersion prepared in Example 13 so that the entire contents can be transferred to a 50 ml conical tube. Adjust the tube volume to 40.0 ml.
Then transfer the contents in 10 ml aliquots to four 15 ml conical tubes.
Dissolve 1000 mg of Pyroxidal-5-phosphate with 800 111 of 10 N NaOH and adjust with water to 10 mls. Filter sterilize this clear yellow solution with a .2 ,um acrodisc and add 2.5 ml aliquots to each of the previously pre-pared 4 brushite tubes. Vortex each tube a few seconds to make certain WO 95/U392 PCT/US9~/12515 that the contents are well dispersed. Lyophilize overnight lapprox. 16 hrs]
at the low drying rate setting. The next morning resuspend in 50 ml ali-quots of sterile HPLC grade water five more times. Pellet once more and transfer the pellets to four 15 ml conical tubes and adjust the final prepa-ration volume with water to 40.0 ml.

Exam~le 23. Coating a Meticulously Clean Surface of Calcium Phos-phate Dihvdrate (Brushite) With a Molecular Stabilizing Film of Citrate:

Brushite/citrate. Pellet the 100 ml of the dispersion prepared in Example 13 so that entire contents can be transferred to a 50 ml conical tube. Adjust the tube volume to 40.0 ml. Then transfer the contents in 10 ml aliquots to four 15 ml conical tubes. Add 10 ml of 100 mM citrate to each of the 15 ml conicals and nutate for 30 minutes at room tempera-ture. Lyophilize overnight [approx. 16 hrs] at the low drying rate setting.
The next morning resuspend in 50 ml aliquots of sterile HPLC grade water five more times. Pellet once more and transfer the pellets to four 15 ml conical tubes and adjust the final preparation volume with water to 40.0 ml.
Examnle 24. Immobilizing Insulin on Brushite:

Insulin Addition. 100 units of insulin is added to each of the four 10 ml suspension prepared in Example 15 and then agitated on a nutator at 4C:
1) Lyophilization: Two of the core preparations are Iyophilized overnight on a Savant Speed Vac (SVC100) under the low drying rate setting for approximately 16 hours. The next morning the Iyophilate is resuspended to 10 ml with HPLC grade sterile water. Three washes with water are performed by pelleting and resuspension. Activity is determined ~ 63-during each wash by removing successive 1.0 ml aliquots and measuring the adsorption of light at 272 nm. Once it is determined that there is no activity in the supernatant (carrier) the preparation will have about 4.0 units per ml for injection. A typical injection is 500 ul [2.0 units].

If desired, the Brushite particles with the insulin immobilized thereon can be encapsulated in phospholipid as follows:
After insulin Iyophilization, bring each of the preparations up to 10.0 ml with a water dispersion of 10% phosphatidyl choline, 10% phosphatidyl serine, and 5% water soluble cholesterol (Sigma Biochemical). Allow the mixture to incubate overnight at 4C on a rocker. The next morning extrude the mixture through a 19 gauge needed without promoting signi-ficant foaming. Then three washes with water are performed by pelleting and resuspension. Activity is determined during each wash by removing successive 1.0 ml aliquots and measuring the adsorption of light at 272 nm. Once it is determined that there is no activity in the supernatant (carrier) the preparation will have about 4 units per ml for injection. A
typical injection is about 500 ul [2.0 units].
In vivo experiments show that intravenous injections of insulin immobilized on the coated brushite particles exhibit the same physiologic activity (serum glucose suppression) as solution phase insulin. Without the coating, insulin loses all biological activity when immobilized on brushite.

Example 25. Immobilizing Bovine Serum Albumin on Zinc-Selenide Coated with Cellobiose: This example describes the modification of the surface of an analytic device used for Fourier transform infrared spectroscopy. The high energy surface of a meticulously cleaned ZnSe specimen holder is modified with a stabilizing film of cellobiose. While this provides a unique test surface for protein conformation analysis, it may be used to improve the biocompatibility of materials as well.

Wo 95/12392 PCI/US94/12515 Sample Prenaration. The surface film coating the ATR specimen holder was comprised of cellobiose, and the analyte was bovine serum albumin. A solution of 100 mM cellobiose (Sigma's D-( + ) cellobiose, FW
= 342.3 solid-form) was prepared in HPLC-grade water (Sigma). Solid bovine serum albumin (Sigma, I\Aol. Wt. 46000) was dissolved in phos-phate buffered saline (Sigma, Dulbeco's Phosphate Buffer, pH = 7.2) to obtain a desired concentration of 4% (w/v). All solutions were used within 14 days of preparation, and were stored at 4C between experiments.

ATR Samnle Holder PreDaration. Prior to layering a thin desiccated film of cellobiose onto a horizontal ZnSe-45 ATR specimen holder (Spectra-Tech model, Stamford, CT), the plate was thoroughly washed with a solution of 100 mM NaCI and 100 mM NaCH03, followed by HPLC-grade water and acetone. A film of cellobiose was adsorbed onto the clean surface of the crystal by evenly applying 400 ul of 100 mM cello-biose and Iyophilizing for ten minutes without applied heat or rotation (Savant SVC 100 Iyophilizer, Wesbury, NY). 100 ul of the 4% BSA solu-tion was then added onto the cellobiose coating. Excess protein solution was removed by gentle aspiration, and both the specimen and FTIR cham-ber were purged with N2 for 15 minutes.
The immobilized BSA was analyzed by ATR-FTIR and found to have the same conformation as unbound aqueous phase BSA in terms of the proportional distribution of secondary structure components.

2~ Examcle 26. Immobilizing Angiotensin Converting Enzyme on a ~arbon Ceramic Core: Angiotensin converting enzyme was immobilized on carbon nanocrystalline particles coated with the procedures described in the previous examples. The rate of substrate cleavage in the solution phase, i.e. formation of BRP's, was found to be increased by a factor of five over the rate of substrate cleavage observed in the solution phase for WO 95/12392 ?.~7 ~ PCT/US94/12515 non-anchored enzyme. The rate of substrate cleavage for angiotensin converting enzyme bound directly to uncoated particles was only 2.5 times that of the non-anchored enzyme.

Example 27. Preparation and Surface Adsorction of Human Serum Transferrin Protein of Brushite Particles Coated With Cellobiose: Brushite nanocrystalline particles are prepared as set forth in Example 9.
To adsorb protein to the cellobiose coated nanocrystalline cores, the core sample is diluted to 10.0 ml with Ca++ and Mg++ free phosphate buf-fered saline (Gibco). Forty (40.0) I~g of purified human serum transferrin (4~g/~l) (Gibco), whose antigenicity is verified by ELISA, is then added to a 10 ml stir cell (Spectra). The sample is then left to stir slowly for 30 minutes, taking great care ~ t to àllow foaming. After the addition period, 15 ml of Ca++ and Mg++ ,ree phosphate buffered saline (Gibco) is then washed through the cell under a 2 psi nitrogen gas pressure head. After washing, the sample is again concentrated to 1.00 ml under N2 and a 500 ,ul sample is removed for analysis by photon correlation spectroscopy, Doppler electrophoretic light scatter and transmission electron microscopy as detailed below.
Conformational integrity is assessed by measuring the retained anti-genicity of the bound protein. To the sample cell, 50.0 ~l of rabbit polyclonal anti-human transferrin antibody (Dako), whose antigenicity is confirmed by ELISA, is added to the concentrated 1.0 ml reaction product at 37.5C with gentle stirring. After a 30 minute incubation period, 15 ml of Ca++ and Mg++ free phosphate buffered saline (Gibco) is then washed through the cell under a 2 psi nitrogen gas pressure head and the reaction volume is again reduced to 1.0 ml.
A 200 /ll aliquot of blocking agent, 1% w/v bovine serum albumin in divalent free saline, is added followed by a 10 minute equilibration period. The secondary antibody, 30 nm gold conjugated goat anti-rabbit -polyclonal IgG (Zymed), is then added and the reaction mixture is allowed to incubate for 30 minutes. A sample is removed, chopped on a transmis-sion electron microscopy grid, and vacuum dried. The mixture is again washed with 15 ml of divalent free saline under a nitrogen pressure head and then fixed with glutaraldehyde. One ml of 3% solid bovine collagen (Collagen Corp. ) is then added to the mixtures and the composite is ultracentrifuged at 106xg for 30 minutes yielding a pellet that is then routinely processed as a biological specimen for transmission electron microscopy.
Following transferring binding, the Brushite/cellobiose/protein conjugates will measure about 150 nm. The biological activity of protein absorbed to the surface of carbohydrate-treated nanocrystalline TCP
particles is preserved.

Examole 28. Preparing Meticulouslv Clean Biodegradable NanoDar-ticles:
1. Prepare 6 clean sonication tubes with 500 mg of biodegradable particles per tube.

2. In fume hood, fill tubes with HCI (10 N) approx. 8 ml/tube.

3. Sonicate for 30 min. (full power [175 wattsl/25C); three tubes per sonication treatment.

4. Centrifuge 30 min. at 2000 rpm.

5. Decant the acidic supernatant (in the fume hood), fill the tubes with HPLC grade water and then vortex.

WO 95112392 5~ PCT/US94/12515 ~ -67-6. Sonicate for 30 min [above conditions] and centrifuge for 30 [centrifuging is complete if the supernatant is clear].

7. Decant the supernatant, and fill the tubes with HPLC grade water and vortex.

8. Repeat steps 7 and 8 two more times.

9. Decant the preparation into a clean glass [pyrex] baking dish.
10. Anneal at 210C overnight.

11. Remove the dried biodegradable crystals by gentle scraping with a clean unpainted spatula and transfer into 6 clean glass sonicating 1 5 tubes.

12. Repeat steps 3 through 8.

13. Prepare a 10 kD (NMWL) 150 ml ultrafiltration cell, empty the contents only one[no more than 500 mg per filtration run] of the tubes into the cell, and wash 500 ml of HPLC grade water through the cell under a N2 pressure head of 20 psi (regulator pressure gauge reading) .

14. After washing, adjust the preparation volume to 100.0 ml by using the appropriate volume markings on the side of the cell.

15. Take a concentration measurement by removing 1.0 ml of the prepa-ration from the cell and Iyophilizing it down in a pre-weighed 1.7 ml Eppendorf tube. After Iyophilization, take a mass measurement of WO 95/12392 PCT/US94/12515 ~
,44 the tube with its contents and subtract it away from the mass of the empty tube. This provides the initial density of the preparation.
Preferably, the concentration or density of the particles in the solution is about 10 mg/ml. If the initial density is lower than 10 mg/ml, then the solution should be further concentrated in the ultrafiltration cell.

Exam~le 29. Coating Meticulouslv Clean Biodegradable Nanoparticles with a Molecular Stabilizing Film (Cellobiose):
Incubation/Lvophilization .
1. Sonicate the meticulously clean biodegradable particles (aqueous dispersion) prepared in Example 7 for 30 minutes at 25C at full power [175 Watts].
2. Then as quickly as possible, exchange suspending medium from water (stock) to a solution of 500 mM cellobiose using either a bench top microcentrifuge (30 seconds, full speed of 14,000 RPM) for small volumes or for larger volumes a floor models centrifuge (model 21 K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes). Suspend the pelleted particles with 500 mM cello-biose, sonicate to aid dispersion (approximately 5 minutes at 25C
at full power [175 Watts]) and finally set the mixture on a rocking plate overnight in a cold room [4C].
3. The next day portion out the mixture into appropriately sized vessels for overnight Iyophilization.

4. Leave the tubes capped with a layer of parafilm around the cap and place them in a freezer until the washing step.

~ -69-5. Reconstitute the particle/cellobiose in a suitable buffer depending on the application. Suitable buffers are low ionic strength buffered phosphate (PRB), water, or bicarbonate. Reconstitution in the buffer is accomplished by vortexing and a 5 minute sonication [175 Watts/
25 C].

6. Wash by repeated centrifugation (using either a bench top micro-centrifuge [30 seconds, full speed of 14,000 RPM] for small volumes or for large volumes a floor model centrifuge [model 21K, in 50 ml centrifuge tubes, 8,000 RPM for a maximum of 2 minutes]) and resuspension into the buffer.

7. Take a concentration measurement by removing 1 ml of the suspen-sion dehydrating it in a Iyophilizer in a pre-weighed 1.7 ml Eppendorf tube, and massing.

8. Calculate the final volume necessary to bring the concentration to 1 mg/ml. Add enough buffer to bring the concentration of the parti-cle/cellobiose preparation to 1 mg/ml.
Example 30. Preparation of Cellobiose Coated Brushite Particle with Hemoqlobin Bound Thereto: The following example demonstrates the preparation of Brushite particle having hemoglobin bound thereto in accord-ance with the present invention. The fabrication process involved coating ultrafine nanocrystalline Brushite particles with a glassy film of disac-charides and then physically adsorbing purified hemoglobin. The assembly was then coated with phospholipid.
One (1.00) g. of ultrafine particles prepared as in Example 28 was dispersed in 5.0 ml of 100 mM cellobiose (Sigma) solution with 175 watt sonication (Branson) for 10 minutes. The colloid was then incubated at W095/12392 PCT/US9~/12515 70_ 4.0C overnight in a 10 kD stir cell. The following day, this colloid was Iyophilized for 24 hours and reconstituted in 1.0 ml of ddH20. Unabsorbed cellobiose was removed by 10 kD stir cell ultra filtration (UF) (Filtron) against 100 ml of 20 mM phosphate buffer (pH 7.4) (PRB) and corrected to 2.0 ml. (UF) (Filtron) against 100 ml of 20 mM phosphate buffer (pH
7.4) (PRB) and corrected to 2.0 ml.
Five hundred (500) mg of human hemoglobin type Ao (Sigma) was dissolved in 5.0 ml PBS (pH 6.8) (Gibco) and then ultrafiltered against 150 ml PRB at 4.0 C in a 50 kD stir with 30 psi N2. The filtrate was adjusted to 3.0 ml. The surface modified Brushite dispersion (2.0 mL) was then added to the 50 kD ultrafiltrate cell and allowed to incubate overnight with slow stirring (5 psi Nz). The filtrate was adjusted to 3.0 ml. The surface modified diamond dispersion (2.0 mL) was then added to the 50 kD ultrafil-trate cell and allowed to incubate overnight with slow stirring (5 psi N2, 4.0C). The next morning, 35 uL of phosphatidyl dipalmitoyl serine (10 mM of 6.0 mM NaOH) (Sigma), 50 uL of phosphatidyl dipalmitoyl choline [6.8 mM stock] Sigma, and 8.7 uL of cholesterol [3.9 mM stock] (Sigma) was stirred in and incubated for approximately 6.0 hours. The final product was again filtered in a 50 kD ultrafiltration cell over 30 psi N2 against 20 ml PBS (pH 7.4, 4.0C and adjusted to 5.0 ml for an estimated hemoglobin concentration of 10 g/dL.

Example 31. Preparation of P5P Coated Brushite Particle with Hemoglobin Bound Thereto: In this example, red blood cell surrogates are made in the same manner as Example 30 except that pyridoxal-5-phosphate is substituted for cellobiose as the oxygen carrier anchor coating.
50 mg of acid cleaned particles was dispersed by 175 watt sonica-tion (Branson) for 10 minutes, mixed with 75.0 mg of pyridoxal-5-phos-phate [Sigma] and adjusted to 10.0 ml with deionized water. The mixture ~ 71 -was spun Iyophilized overnight, washed with 4-10 ml aliquots of deionized water and reconstituted to 25 mg/ml in pH 7.40, 20 mM phosphate buffer.
4.0 ml [25 mg/ml~ of nanocrystalline core particles were added to 1.0 ml recovered red blood Iysate hemoglobin [26.10 g/dL]. The mixture was then slowly dialized into 100 ml of 0.5X PRB overnight at 4.0C
under a nitrogen head of 20 psi and through a 10 kD ultrafiltration cell.
The next morning,34 uL of phosphatidyl serine [10 mM of 6.0 mM NaOH]
(Sigma), 50 uL of cholesterol [3.9 mM stock] (Sigma) was stirred in and incubated for approximately 6.0 hours. The final product was again filtered to remove free hemoglobin in a 50 kD ultrafiltration cell over 30 psi N2 against 200 ml PBS (pH 7.4, 4.0C) and adjusted to 1.0 - 2.5 ml or an estimated hemoglobin concentration of 10 g/dL.
The preceding procedure was repeated at different pyridoxal-5-phosphate concentrations. As a result, red blood cell surrogates were prepared wherein the nanocrystalline particles were coated in solutions containing 1 mM pyridoxal-5-phosphateand 30 mM pyridoxal-5-phosphate.
The red blood cell surrogates prepared in Examples 30 and 31 were analyzed for oxygen dissociation characteristics as well as size distribution, electrophoretic mobility and retained molecular conformation. The red blood cell surrogates coated with cellobiose (Example 30) exhibited an oxygen dissociation (P50) of about 26-30 mm Hg. The red blood cell surrogates having coatings of pyroxidal-5-phosphate should have oxygen dissociations (P50) of about 37 mm Hg. This compares well with the oxygen dissociation of whole human blood which is 31 mm Hg. In addi-tion, hemoglobin-bound nanocrystalline particles were prepared in the same manner as Example 30 except that the coating of lipid was deleted. The oxygen-dissociation of the lipid-free hemoglobin-bound nanocrystalline particles was about 10 mm Hg.
The electrophoretic mobility of the red blood cell surrogates produced in Examples 30 and 31 were measured with Doppler electro-phoretic light scatter analysis (DELSA 440, Coulter Electronics, Inc., Hialeah, FL). The electrophoretic mobility was -1.7 um cmtVs at a pH of 7.4 PRGB BUFFER at 25C.
The size distribution of the red blood cell surrogates produced in Examples 30 and 31 were measured by both photon correlation spectro-scopy at a 90 angle in PRB buffer at 22.5C (N4MD, Coulter) and by transmission microscopy (TEM, Zeiss 190). The red blood cell surrogates measured about 200 nanometers by photon correlation. For electron microscopy, a 10 microliter drop of particles in solution was placed on a paraffin surface which included a carbon-stabilized FORMVAR GRID (Ted Pella, Inc., Redding, CA) which was floated on top of the drop. Due to the high surface charge of the TEM GRID, the red blood cell surrogates absorbed to the grid allowing excess solution to be removed by careful blotting. A simiiar method was then used to stain the particles with 2%
phosphotungstic acid. The stained grid was then dried and the red blood cell surrogates identified as having particle sizes in the range of 50-100 nanometers.
The conformational integrity of the red blood cell surrogates was verified by immunogold antibody affinity intensity. After being deposited on one nanometer TEM copper grids, the protein bound particles were incu-bated for one hour at 27C with polyclonal rabbit anti-human hemoglobin antibodies (Dako) and secondary goat anti-rabbit 30 nanometer gold-labeled antibodies (Zymed Laboratories, San Francisco, CA). The gold-labeled anti-bodies were observed to attach avidly to the hemoglobin present in the red blood cell surrogates.

ExamDle 32. PreDaration of P5P Coated Brushite Particle with Taxol Bound Thereto: In this example, drug delivery vehicles are made in the same manner as Example 30 except that taxol is substituted for hemo-globin.

WO 95112392 ~?a~ PCT/US94/12515 50 mg of cleaned particles was dispersed by 175 watt sonication (Branson) for 10 minutes, mixed with 75.0 mg of pyridoxal-5-phosphate [Sigma] and adjusted to 10.0 ml with deionized water. The mixture was spun Iyophilized overnight, washed with 4-10 ml aliquots of deionized water and reconstituted to 25 mg/ml in pH 7.40, 20 mM phosphate buffer.
4.0 ml [25 mg/ml] of nanocrystalline core particles were added to 1.0 ml solubilized taxol (100 mg/10 ml dd H2O. The mixture was then slowly dialized into 100 ml of 0.5X PRB overnight at 4.0C under a nitrogen head of 20 psi and through a 10 kD ultrafiltration cell and then Iyophilized. The next morning,34 uL of phosphatidyl serine [10 mM of 6.0 mM NaOH] (Sigma), 50 uL of cholesterol [3.9 mM stock] (Sigma) was stirred in and incubated for approximately 6.0 hours. The final product was again filtered in a 50 kD ultrafiltration cell over 30 psi N2 against 200 ml PBS (pH 7.4, 4.0C).
Example 33: Attachment of Transfection Agents:
The following procedure is used to attach DNA or RNA segments to any of the coated particles described in the preceding Examples:
A dispersion of 40 mg/ml of coated nanoparticles in 20 mM pH 6.80 phosphate buffer is prepared. To this dispersion, 1.00 ug of cesium chloride purified DNA or RNA fragments are added and left to absorb at room temperature under mild agitation for approximately 16 hours Specific DNA fragment used in this example is the human deaminase gene Example 34: Attachment of Targeting Ligands and Phoscholipid Membrane: The procedure described in the example for isolating and attaching the targeting ligands and phospholipid membrane may be used for all of the particle/coating/DNA or RNA combinations set forth in the preceding examples.

WO 95/12392 PCTIUS9411251~i 10~ transforming units of virus is incubated with Triton extraction buffer (1.0% of Triton X 100/0.25 mM Dm10 mM Tris pH 7.4\ 1.0 mM
MgCI). Extract is then ultracentrifuged at 100K*g for 2.0 hrs (35 rpm SW50.1 Beckman rotor) at 4.0C to remove nucleocapsid. Removal of triton and envelope protein enrichment is accomplished by incubation with a 300 ul slurry of polystyrene micro beads (Spectra Gel D2) and subse-quent 100 kD ultrafiltration into phosphate buffer 5mg/ml of phosphotidyl choline and 5mg/ml phosphotidyl serine. An alternative method of viral extraction is as follows. A mixture of phosphotidyl choline (5mg/ml) and phosphotidyl serine (5mg/ml) in 20 mM pH 7.4 phosphate buffer is soni-cated for 30 minutes at 4.0C. 10~ units per ml of virus is then added to 1.0 ml of the phospholipid mixture and left to sonicate at 5 sec cycles per minute for 30 minute~at 4.0C. Nucleocapsid is removed by ultracentri-fugation at 35krpm at 4.0C. Intervening layers of carbohydrate may be first adsorbed to the TCP-DNA/RNA complex prior to the addition of the ligand/membrane components.

Exam~le 35:
A construct is composed of a brushite core with an adsorbed first layer consisting of human deaminase gene in an expression cassette with a albumin enhancer and limited terminal repeats for genomic integration.
The construct includes an adsorbed second layer of cellobiose and an adsorbed third layer of membrane proteins from human low density lipids (LDL receptor ligands).
The entire contents of all references cited hereinabove are hereby incorporated by reference.

-WO 9~i/12392 C~ s PCT/US94/12515 Having thus described exemplary embodiments of the present inven-tion, it should be noted by those skilled in the art that the within disclosures are exemplary only and that various other alternatives, adaptations and modifications may be made within the scope of the present invention. Accordingly, the present invention is not limited to the specific embodiments as illustrated herein, but is only limited by the following claims.

Claims (31)

What is Claimed is:

1. A composition of matter comprising:
an article having a surface consisting of a metal, metal alloy, polymer, ceramic, glass or intermetallic;
a coating consisting essentially of a material which provides a threshold surface energy to said surface which is sufficient to bind biochemically reactive pairs without denaturing said pairs; and at least one biochemically reactive pair bound to said coated surface wherein said biochemically reactive pair bound to said coated surface is not denatured.

2. A composition of matter according to claim 1 wherein said article is a core particle having a diameter of less than about 1000 nanometers.

3. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group consisting of ligand-receptor pairs, enzyme-substrate pairs, drug-receptor pairs, catalyst-reactant pairs, immunological pairs toxin-ligand pairs, absorbant-absorbate pairs and adsorbent-adsorbate pairs.

4. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group of ligand-receptor pairs consisting of lectins and lectin binding sites, HDL and HDL receptor cellular receptor site, hormones and hormone receptor sites, antibiotics and ribosomal proteins.

5. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group of immunological pair members.

6. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group of enzyme-substrate pairs consisting of lysozyme-chitin pairs, ribonuclease-RNA pairs, carboxy-peptidase A-carboxyl terminal polypeptide pairs, serine, zinc, thiol and carboxyl proteases-protein pairs, NADH-Q reductase-NADH pairs, gluta-thione seductase-glutathione pairs and acetylcholinesterase-acetylcholine pairs.

7. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group of drug-receptor pairs consisting of epinephrine and adrenergic receptors, methadone and opiate receptors.

8. A composition of matter according to claim 1 wherein said bioreactive pair is selected from the group of toxin-ligand pairs consisting of strychnine and the glycine receptor, hemoglobin and carbon monoxide, and organophosphate compounds and acetylcholinesterase.

9. A composition of matter comprising:
a biodegradable core particle having a diameter of less than about 1000 nanometers;
a coating comprising a substance that provides a threshold surface energy to said core particle which is sufficient to bind bio-chemically active agents without denaturing said agents, said substance covering at least a part of the surface of said core particle; and at least one biochemically active agent bound to said coated core particle wherein said biologically active agent bound to said coated core particle is not denatured.

10. A composition of matter according to claim 9 wherein said core particle comprises a biodegradable ceramic or polymer.

11. A composition of matter according to claim 10 wherein said core particle comprises a biodegradable ceramic selected from the group consisting of brushite and tricalcium phosphate.

12. A composition of matter according to claim 9 wherein said composition further comprises an outer coating of a phospholipid which comprises target ligands.

13. A composition of matter according to claim 9 wherein said biochemically active agent is one or both members of a bioreactive pair.

14. A composition of matter according to claim 9 wherein said coating comprises citrate, cellobiose or pyridoxal-5-phosphate.

15. A composition of matter according to claim 9 wherein said biologically active agent is a viral protein fragment.

16. A composition of matter according to claim 12 wherein said biologically active agent is a drug.

17. A composition of matter according to claim 9 wherein said biochemically active agent is hemoglobin.

18. A composition of matter comprising:
a biodegradable core particle having a diameter of less than about 1000 nanometers;

a coating comprising a substance that provides a threshold surface energy to said core particle which is sufficient to bind transfection agents without denaturing said agents, said substance covering at least a part of the surface of said core particle; and at least one transfection active agent bound to said coated core particle wherein said transfection agent bound to said coated core particle is not denatured.

19. A composition of matter according to claim 18 wherein said core particle comprises a biodegradable ceramic or polymer.

20. A composition of matter according to claim 19 wherein said core particle comprises of brushite.

21. A composition of matter according to claim 18 which further includes a target coating covering at least a portion of the surface of said core particle.

22. A composition of matter according to claim 21 wherein said outer target coating comprises a phospholipid and targeting ligand.

23. A composition of matter according to claim 18 wherein said coating comprises cellobiose pyridoxal-5-phosphate or citrate.

24. A composition of matter according to claim 18 wherein said transfection agent is a DNA or RNA segment.

25. A composition of matter according to claim 18 wherein said transfection agent is an antisense fragment.

26. A composition of matter according to claim 22 wherein said targeting ligand is a viral envelope.

27. A composition of matter according to claim 21 wherein said core particle comprises a biodegradable ceramic or polymer.

28. A composition of matter according to claim 21 wherein said core particle comprises of brushite.

29. A composition of matter according to claim 21 wherein said coating comprises cellobiose pyridoxal-5-phosphate or citrate.

30. A composition of matter according to claim 21 wherein said transfection agent is a DNA or RNA segment.

31. A composition of matter according to claim 21 wherein said transfection agent is an antisense fragment.