CA2091243A1 - Lyase recombinante acc - Google Patents

Lyase recombinante acc

Info

Publication number
CA2091243A1
CA2091243A1 CA2091243A CA2091243A CA2091243A1 CA 2091243 A1 CA2091243 A1 CA 2091243A1 CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A1 CA2091243 A1 CA 2091243A1
Authority
CA
Canada
Prior art keywords
acc synthase
acc
recombinant
ethylene
effected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2091243A
Other languages
English (en)
Other versions
CA2091243C (fr
Inventor
Athanasios Theologis
Takahide Sato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Commerce
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2091243A1 publication Critical patent/CA2091243A1/fr
Application granted granted Critical
Publication of CA2091243C publication Critical patent/CA2091243C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'isolation de l'ADNc codant la synthase de l'enzyme ACC de zucchini au moyen d'un nouveau procédé d'isolation, permet de récupérer des gènes de synthase de l'ACC à partir d'une grande variété de sources de plantes supérieures. Les synthases de l'ACC sont codées par des familles à gènes multiples dont les membres peuvent être responsables de caractéristiques diverses du développement des plantes induites par l'éthylène. L'utilisation de ce gène permet également la production recombinée de la synthase de ACC et, de ce fait, la production in vitro de l'ACC, d'éthylène et d'éthanol. De plus, on peut maîtriser les processus induits dans les plantes par la synthase de l'ACC, en appliquant une technique de recombinaison non codante ou en utilisant des gènes mutés de synthase de l'ACC.
CA002091243A 1990-09-10 1991-09-10 Lyase recombinante acc Expired - Fee Related CA2091243C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57989690A 1990-09-10 1990-09-10
US07/579,896 1990-09-10
PCT/US1991/006453 WO1992004456A1 (fr) 1990-09-10 1991-09-10 Synthase recombinee de l'enzyme acc (acide 1-aminocyclopropane-1-carboxylique)

Publications (2)

Publication Number Publication Date
CA2091243A1 true CA2091243A1 (fr) 1992-03-11
CA2091243C CA2091243C (fr) 2000-11-28

Family

ID=24318792

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002091243A Expired - Fee Related CA2091243C (fr) 1990-09-10 1991-09-10 Lyase recombinante acc

Country Status (6)

Country Link
EP (1) EP0548164A4 (fr)
JP (1) JPH06502759A (fr)
AU (1) AU657276B2 (fr)
CA (1) CA2091243C (fr)
MX (1) MX9100993A (fr)
WO (1) WO1992004456A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5670454A (en) * 1994-12-15 1997-09-23 Basf Aktiengesellschaft Herbicides of the auxin type for treating transgenic crop plants

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL107239A0 (en) * 1992-10-15 1994-01-25 Gen Hospital Corp Crucifer acc synthase polypeptide, methods for the production thereof and uses thereof
EP0779926A1 (fr) * 1994-09-02 1997-06-25 Seminis Vegetable Seeds, Inc. Plantes transgeniques exprimant les genes de l'oxydase de l'acide 1-aminocyclopropane-1-carboxylique
US5998702A (en) * 1994-12-30 1999-12-07 Seminis Vegetable Seeds, Inc. Transgenic plants expressing ACC synthase gene
US6448474B1 (en) 1995-06-07 2002-09-10 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants
US5874269A (en) * 1995-06-07 1999-02-23 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plant
US6043409A (en) * 1995-06-07 2000-03-28 Seminis Vegetable Seeds, Inc. Transgenic plants expressing ACC oxidase genes
AU727323B2 (en) * 1995-09-20 2000-12-07 University Of Queensland, The Novel ACC synthase genes
US6075184A (en) * 1996-03-26 2000-06-13 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
AUPN958296A0 (en) 1996-05-01 1996-05-23 Golden Circle Limited Novel acc synthase genes from pineapple
US5952546A (en) * 1996-06-27 1999-09-14 Dna Plant Technology Corporation Delayed ripening tomato plants with T-DNA bearing a truncated ACC2 synthase gene
CA2287777A1 (fr) * 1997-04-09 1998-10-15 The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty' S Government Of The United Kingdom Of Great Britain And Northern Ireland Promoteurs de plantes activables
EP1555317B1 (fr) 1998-03-20 2011-09-28 Commonwealth Scientific And Industrial Research Organisation Genes synthetiques et constructions genetiques comprenant ces genes
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
US8598332B1 (en) 1998-04-08 2013-12-03 Bayer Cropscience N.V. Methods and means for obtaining modified phenotypes
EP2267139B1 (fr) 1998-04-08 2017-03-22 Commonwealth Scientific and Industrial Research Organisation Procédés et moyens d'obtention de phénotypes modifiés
US20040214330A1 (en) 1999-04-07 2004-10-28 Waterhouse Peter Michael Methods and means for obtaining modified phenotypes
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
CN1154745C (zh) * 1999-11-09 2004-06-23 浙江省农业科学院 利用反义基因调控籽粒油脂含量的方法
DE10212892A1 (de) 2002-03-20 2003-10-09 Basf Plant Science Gmbh Konstrukte und Verfahren zur Regulation der Genexpression
HUE035897T2 (en) 2003-06-23 2018-05-28 Pioneer Hi Bred Int Incorporating the green-capability of a single gene-controlled option into the plant
EP2980220A1 (fr) 2005-09-20 2016-02-03 BASF Plant Science GmbH Procédés améliorés de contrôle de l'expression de gènes
US9315818B2 (en) 2006-06-07 2016-04-19 Yissum Research Development Company Of The Hebrew University Of Jerusalem Plant expression constructs and methods of utilizing same
US8975470B2 (en) 2009-06-30 2015-03-10 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Introducing DNA into plant cells
BRPI1105206B1 (pt) * 2011-11-30 2021-06-01 Universidade Estadual De Campinas - Unicamp Método para produção de plantas geneticamente modificadas com capacidade de controle da produção do hormônio etileno, plantas geneticamente modificadas assim obtidas, vetor de dna recombinante e seus usos
WO2013184768A1 (fr) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions et méthodes d'inactivation génique dans les plantes
CA2891720C (fr) 2012-11-21 2022-05-03 Nunhems B.V. Plantes de solanum lycopersicum presentant des alterations non-transgeniques dans le gene acs2
US20150040268A1 (en) 2013-04-25 2015-02-05 Morflora Israel Ltd Methods and compositions for the delivery of nucleic acids to seeds

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) * 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US4801540A (en) * 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
GB8916213D0 (en) * 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5670454A (en) * 1994-12-15 1997-09-23 Basf Aktiengesellschaft Herbicides of the auxin type for treating transgenic crop plants

Also Published As

Publication number Publication date
EP0548164A4 (en) 1994-06-08
EP0548164A1 (fr) 1993-06-30
JPH06502759A (ja) 1994-03-31
WO1992004456A1 (fr) 1992-03-19
MX9100993A (es) 1992-05-04
CA2091243C (fr) 2000-11-28
AU8511491A (en) 1992-03-30
AU657276B2 (en) 1995-03-09

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