CA2091243A1 - Lyase recombinante acc - Google Patents
Lyase recombinante accInfo
- Publication number
- CA2091243A1 CA2091243A1 CA2091243A CA2091243A CA2091243A1 CA 2091243 A1 CA2091243 A1 CA 2091243A1 CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A1 CA2091243 A1 CA 2091243A1
- Authority
- CA
- Canada
- Prior art keywords
- acc synthase
- acc
- recombinant
- ethylene
- effected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'isolation de l'ADNc codant la synthase de l'enzyme ACC de zucchini au moyen d'un nouveau procédé d'isolation, permet de récupérer des gènes de synthase de l'ACC à partir d'une grande variété de sources de plantes supérieures. Les synthases de l'ACC sont codées par des familles à gènes multiples dont les membres peuvent être responsables de caractéristiques diverses du développement des plantes induites par l'éthylène. L'utilisation de ce gène permet également la production recombinée de la synthase de ACC et, de ce fait, la production in vitro de l'ACC, d'éthylène et d'éthanol. De plus, on peut maîtriser les processus induits dans les plantes par la synthase de l'ACC, en appliquant une technique de recombinaison non codante ou en utilisant des gènes mutés de synthase de l'ACC.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US57989690A | 1990-09-10 | 1990-09-10 | |
US07/579,896 | 1990-09-10 | ||
PCT/US1991/006453 WO1992004456A1 (fr) | 1990-09-10 | 1991-09-10 | Synthase recombinee de l'enzyme acc (acide 1-aminocyclopropane-1-carboxylique) |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2091243A1 true CA2091243A1 (fr) | 1992-03-11 |
CA2091243C CA2091243C (fr) | 2000-11-28 |
Family
ID=24318792
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002091243A Expired - Fee Related CA2091243C (fr) | 1990-09-10 | 1991-09-10 | Lyase recombinante acc |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0548164A4 (fr) |
JP (1) | JPH06502759A (fr) |
AU (1) | AU657276B2 (fr) |
CA (1) | CA2091243C (fr) |
MX (1) | MX9100993A (fr) |
WO (1) | WO1992004456A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5670454A (en) * | 1994-12-15 | 1997-09-23 | Basf Aktiengesellschaft | Herbicides of the auxin type for treating transgenic crop plants |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL107239A0 (en) * | 1992-10-15 | 1994-01-25 | Gen Hospital Corp | Crucifer acc synthase polypeptide, methods for the production thereof and uses thereof |
EP0779926A1 (fr) * | 1994-09-02 | 1997-06-25 | Seminis Vegetable Seeds, Inc. | Plantes transgeniques exprimant les genes de l'oxydase de l'acide 1-aminocyclopropane-1-carboxylique |
US5998702A (en) * | 1994-12-30 | 1999-12-07 | Seminis Vegetable Seeds, Inc. | Transgenic plants expressing ACC synthase gene |
US6448474B1 (en) | 1995-06-07 | 2002-09-10 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants |
US5874269A (en) * | 1995-06-07 | 1999-02-23 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plant |
US6043409A (en) * | 1995-06-07 | 2000-03-28 | Seminis Vegetable Seeds, Inc. | Transgenic plants expressing ACC oxidase genes |
AU727323B2 (en) * | 1995-09-20 | 2000-12-07 | University Of Queensland, The | Novel ACC synthase genes |
US6075184A (en) * | 1996-03-26 | 2000-06-13 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages |
AUPN958296A0 (en) | 1996-05-01 | 1996-05-23 | Golden Circle Limited | Novel acc synthase genes from pineapple |
US5952546A (en) * | 1996-06-27 | 1999-09-14 | Dna Plant Technology Corporation | Delayed ripening tomato plants with T-DNA bearing a truncated ACC2 synthase gene |
CA2287777A1 (fr) * | 1997-04-09 | 1998-10-15 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty' S Government Of The United Kingdom Of Great Britain And Northern Ireland | Promoteurs de plantes activables |
EP1555317B1 (fr) | 1998-03-20 | 2011-09-28 | Commonwealth Scientific And Industrial Research Organisation | Genes synthetiques et constructions genetiques comprenant ces genes |
AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
US8598332B1 (en) | 1998-04-08 | 2013-12-03 | Bayer Cropscience N.V. | Methods and means for obtaining modified phenotypes |
EP2267139B1 (fr) | 1998-04-08 | 2017-03-22 | Commonwealth Scientific and Industrial Research Organisation | Procédés et moyens d'obtention de phénotypes modifiés |
US20040214330A1 (en) | 1999-04-07 | 2004-10-28 | Waterhouse Peter Michael | Methods and means for obtaining modified phenotypes |
US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
CN1154745C (zh) * | 1999-11-09 | 2004-06-23 | 浙江省农业科学院 | 利用反义基因调控籽粒油脂含量的方法 |
DE10212892A1 (de) | 2002-03-20 | 2003-10-09 | Basf Plant Science Gmbh | Konstrukte und Verfahren zur Regulation der Genexpression |
HUE035897T2 (en) | 2003-06-23 | 2018-05-28 | Pioneer Hi Bred Int | Incorporating the green-capability of a single gene-controlled option into the plant |
EP2980220A1 (fr) | 2005-09-20 | 2016-02-03 | BASF Plant Science GmbH | Procédés améliorés de contrôle de l'expression de gènes |
US9315818B2 (en) | 2006-06-07 | 2016-04-19 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Plant expression constructs and methods of utilizing same |
US8975470B2 (en) | 2009-06-30 | 2015-03-10 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Introducing DNA into plant cells |
BRPI1105206B1 (pt) * | 2011-11-30 | 2021-06-01 | Universidade Estadual De Campinas - Unicamp | Método para produção de plantas geneticamente modificadas com capacidade de controle da produção do hormônio etileno, plantas geneticamente modificadas assim obtidas, vetor de dna recombinante e seus usos |
WO2013184768A1 (fr) | 2012-06-05 | 2013-12-12 | University Of Georgia Research Foundation, Inc. | Compositions et méthodes d'inactivation génique dans les plantes |
CA2891720C (fr) | 2012-11-21 | 2022-05-03 | Nunhems B.V. | Plantes de solanum lycopersicum presentant des alterations non-transgeniques dans le gene acs2 |
US20150040268A1 (en) | 2013-04-25 | 2015-02-05 | Morflora Israel Ltd | Methods and compositions for the delivery of nucleic acids to seeds |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4535060A (en) * | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
US4801540A (en) * | 1986-10-17 | 1989-01-31 | Calgene, Inc. | PG gene and its use in plants |
GB8916213D0 (en) * | 1989-07-14 | 1989-08-31 | Ici Plc | Dna constructs,cells and plants derived therefrom |
-
1991
- 1991-09-09 MX MX9100993A patent/MX9100993A/es unknown
- 1991-09-10 WO PCT/US1991/006453 patent/WO1992004456A1/fr not_active Application Discontinuation
- 1991-09-10 JP JP3515173A patent/JPH06502759A/ja active Pending
- 1991-09-10 EP EP19910916215 patent/EP0548164A4/en not_active Ceased
- 1991-09-10 AU AU85114/91A patent/AU657276B2/en not_active Ceased
- 1991-09-10 CA CA002091243A patent/CA2091243C/fr not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5670454A (en) * | 1994-12-15 | 1997-09-23 | Basf Aktiengesellschaft | Herbicides of the auxin type for treating transgenic crop plants |
Also Published As
Publication number | Publication date |
---|---|
EP0548164A4 (en) | 1994-06-08 |
EP0548164A1 (fr) | 1993-06-30 |
JPH06502759A (ja) | 1994-03-31 |
WO1992004456A1 (fr) | 1992-03-19 |
MX9100993A (es) | 1992-05-04 |
CA2091243C (fr) | 2000-11-28 |
AU8511491A (en) | 1992-03-30 |
AU657276B2 (en) | 1995-03-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |