CA2091243A1 - Recombinant acc synthase - Google Patents
Recombinant acc synthaseInfo
- Publication number
- CA2091243A1 CA2091243A1 CA2091243A CA2091243A CA2091243A1 CA 2091243 A1 CA2091243 A1 CA 2091243A1 CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A CA2091243 A CA 2091243A CA 2091243 A1 CA2091243 A1 CA 2091243A1
- Authority
- CA
- Canada
- Prior art keywords
- acc synthase
- acc
- recombinant
- ethylene
- effected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Isolation of the cDNA encoding the ACC synthase of zucchini using a novel isolation method permits the recovery of ACC synthase genes from a variety of higher plant sources. The ACC synthases are coded by multigene families, the members of which may be responsible for various plant development characteristics effected by-ethylene. Recombinant production of ACC
synthase and thereby in vitro production of ACC, ethylene and ethanol is also enabled by use of this gene. In addition, control of the processes in plants which are mediated by ACC synthase can be effected using antisense technology or by the use of mutated ACC synthase genes.
synthase and thereby in vitro production of ACC, ethylene and ethanol is also enabled by use of this gene. In addition, control of the processes in plants which are mediated by ACC synthase can be effected using antisense technology or by the use of mutated ACC synthase genes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US57989690A | 1990-09-10 | 1990-09-10 | |
US07/579,896 | 1990-09-10 | ||
PCT/US1991/006453 WO1992004456A1 (en) | 1990-09-10 | 1991-09-10 | Recombinant acc synthase |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2091243A1 true CA2091243A1 (en) | 1992-03-11 |
CA2091243C CA2091243C (en) | 2000-11-28 |
Family
ID=24318792
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002091243A Expired - Fee Related CA2091243C (en) | 1990-09-10 | 1991-09-10 | Recombinant acc synthase |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0548164A4 (en) |
JP (1) | JPH06502759A (en) |
AU (1) | AU657276B2 (en) |
CA (1) | CA2091243C (en) |
MX (1) | MX9100993A (en) |
WO (1) | WO1992004456A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5670454A (en) * | 1994-12-15 | 1997-09-23 | Basf Aktiengesellschaft | Herbicides of the auxin type for treating transgenic crop plants |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL107239A0 (en) * | 1992-10-15 | 1994-01-25 | Gen Hospital Corp | Crucifer acc synthase polypeptide, methods for the production thereof and uses thereof |
CA2198708A1 (en) * | 1994-09-02 | 1996-03-14 | Maury L. Boeshore | Transgenic plants expressing acc oxidase genes |
WO1996021027A1 (en) * | 1994-12-30 | 1996-07-11 | Asgrow Seed Company | Transgenic plants expressing acc synthase gene |
US6043409A (en) * | 1995-06-07 | 2000-03-28 | Seminis Vegetable Seeds, Inc. | Transgenic plants expressing ACC oxidase genes |
US6448474B1 (en) | 1995-06-07 | 2002-09-10 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants |
US5874269A (en) * | 1995-06-07 | 1999-02-23 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plant |
ATE408007T1 (en) | 1995-09-20 | 2008-09-15 | Univ Queensland | NUCLEOTIDE SEQUENCES THAT CODE FOR ACC SYNTHASE ENZYMES FROM PAPAYA |
US6075184A (en) * | 1996-03-26 | 2000-06-13 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages |
AUPN958296A0 (en) | 1996-05-01 | 1996-05-23 | Golden Circle Limited | Novel acc synthase genes from pineapple |
US5952546A (en) * | 1996-06-27 | 1999-09-14 | Dna Plant Technology Corporation | Delayed ripening tomato plants with T-DNA bearing a truncated ACC2 synthase gene |
NZ500741A (en) * | 1997-04-09 | 2001-06-29 | Ministry Of Agriculture And Fo | Inducible plant promoters selected from apple beta-galactosidase (ABG1) or 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) |
AUPP249298A0 (en) | 1998-03-20 | 1998-04-23 | Ag-Gene Australia Limited | Synthetic genes and genetic constructs comprising same I |
CN1796556A (en) | 1998-03-20 | 2006-07-05 | 联邦科学和工业研究组织 | Control of gene expression |
US8598332B1 (en) | 1998-04-08 | 2013-12-03 | Bayer Cropscience N.V. | Methods and means for obtaining modified phenotypes |
US20040214330A1 (en) | 1999-04-07 | 2004-10-28 | Waterhouse Peter Michael | Methods and means for obtaining modified phenotypes |
CA2325344C (en) | 1998-04-08 | 2017-12-05 | Commonwealth Scientific And Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
CN1154745C (en) * | 1999-11-09 | 2004-06-23 | 浙江省农业科学院 | Method of utilizing antisense gene in controlling protein and oil content of seed |
DE10212892A1 (en) | 2002-03-20 | 2003-10-09 | Basf Plant Science Gmbh | Constructs and methods for regulating gene expression |
CN1871346B (en) | 2003-06-23 | 2013-05-29 | 先锋高级育种国际公司 | Engineering single-gene-controlled staygreen potential into plants |
EP1931789B1 (en) | 2005-09-20 | 2016-05-04 | BASF Plant Science GmbH | Methods for controlling gene expression using ta-siran |
US9315818B2 (en) | 2006-06-07 | 2016-04-19 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Plant expression constructs and methods of utilizing same |
CA2766918A1 (en) | 2009-06-30 | 2011-01-06 | Ilan Sela | Introducing dna into plant cells |
BRPI1105206B1 (en) * | 2011-11-30 | 2021-06-01 | Universidade Estadual De Campinas - Unicamp | METHOD FOR THE PRODUCTION OF GENETICALLY MODIFIED PLANTS WITH ABILITY TO CONTROL THE PRODUCTION OF ETHYLENE HORMONE, GENETICALLY MODIFIED PLANTS SO OBTAINED, RECOMBINANT DNA VECTOR AND ITS USES |
WO2013184768A1 (en) | 2012-06-05 | 2013-12-12 | University Of Georgia Research Foundation, Inc. | Compositions and methods of gene silencing in plants |
WO2014079896A1 (en) | 2012-11-21 | 2014-05-30 | Nunhems B.V. | Solanum lycopersicum plants having non-transgenic alterations in the acs2 gene |
US20150040268A1 (en) | 2013-04-25 | 2015-02-05 | Morflora Israel Ltd | Methods and compositions for the delivery of nucleic acids to seeds |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4535060A (en) * | 1983-01-05 | 1985-08-13 | Calgene, Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use |
US4801540A (en) * | 1986-10-17 | 1989-01-31 | Calgene, Inc. | PG gene and its use in plants |
GB8916213D0 (en) * | 1989-07-14 | 1989-08-31 | Ici Plc | Dna constructs,cells and plants derived therefrom |
-
1991
- 1991-09-09 MX MX9100993A patent/MX9100993A/en unknown
- 1991-09-10 JP JP3515173A patent/JPH06502759A/en active Pending
- 1991-09-10 CA CA002091243A patent/CA2091243C/en not_active Expired - Fee Related
- 1991-09-10 EP EP19910916215 patent/EP0548164A4/en not_active Ceased
- 1991-09-10 WO PCT/US1991/006453 patent/WO1992004456A1/en not_active Application Discontinuation
- 1991-09-10 AU AU85114/91A patent/AU657276B2/en not_active Ceased
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5670454A (en) * | 1994-12-15 | 1997-09-23 | Basf Aktiengesellschaft | Herbicides of the auxin type for treating transgenic crop plants |
Also Published As
Publication number | Publication date |
---|---|
AU657276B2 (en) | 1995-03-09 |
WO1992004456A1 (en) | 1992-03-19 |
EP0548164A1 (en) | 1993-06-30 |
EP0548164A4 (en) | 1994-06-08 |
JPH06502759A (en) | 1994-03-31 |
MX9100993A (en) | 1992-05-04 |
AU8511491A (en) | 1992-03-30 |
CA2091243C (en) | 2000-11-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |