BR9911023A - Método para separar magneticamente partìculas selecionadas, particularmente células biológicas e aparelho para separar magneticamente partìculas selecionadas - Google Patents

Método para separar magneticamente partìculas selecionadas, particularmente células biológicas e aparelho para separar magneticamente partìculas selecionadas

Info

Publication number
BR9911023A
BR9911023A BR9911023-7A BR9911023A BR9911023A BR 9911023 A BR9911023 A BR 9911023A BR 9911023 A BR9911023 A BR 9911023A BR 9911023 A BR9911023 A BR 9911023A
Authority
BR
Brazil
Prior art keywords
tube
buffer liquid
sample
magnetically separating
target particles
Prior art date
Application number
BR9911023-7A
Other languages
English (en)
Other versions
BR9911023B1 (pt
Inventor
Chaim Davidson
Ofer Klein
Aharon Lamish
Original Assignee
Chaim Davidson
Ofer Klein
Aharon Lamish
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chaim Davidson, Ofer Klein, Aharon Lamish filed Critical Chaim Davidson
Publication of BR9911023A publication Critical patent/BR9911023A/pt
Publication of BR9911023B1 publication Critical patent/BR9911023B1/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/035—Open gradient magnetic separators, i.e. separators in which the gap is unobstructed, characterised by the configuration of the gap
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/30—Combinations with other devices, not otherwise provided for

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

"MéTODO PARA SEPARAR MAGNETICAMENTE PARTìCULAS SELECIONADAS, PARTICULARMENTE CéLULAS BIOLóGICAS E APARELHO PARA SEPARAR MAGNETICAMENTE PARTìCULAS SELECIONADAS", compreendendo um método e aparelho para separar magneticamente partículas-alvo de uma amostra para poder produzir uma concentração das partículas-alvo numa amostra, ou uma depleção da amostra em relação às partículas-alvo, produzindo uma mistura da amostra com partículas magnéticas que possuem uma afinidade seletiva para carregar magneticamente as partículas-alvo, alimentando um líquido tampão através de um tubo (12) que inclui uma entrada (12a) conectável a uma fonte de líquido tampão e uma saída para líquido tampão; introduzindo a mistura de amostra dentro do líquido tampão de modo que o líquido tampão forme um portador líquido contínuo para mistura com a amostra conforme ambos são alimentados através de tubo; e aplicando um campo magnético sobre o tubo (12) numa estação magnetizadora (13) no mesmo para fazer com que as partículas-alvo magneticamente marcadas sejam separadas e retidas no líquido tampão dentro do tubo (12) na estação magnetizadora.
BRPI9911023-7A 1998-05-17 1999-05-13 processo e aparelho para separar magneticamente partìculas selecionadas. BR9911023B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL124514 1998-05-17
IL12451498A IL124514A (en) 1998-05-17 1998-05-17 Method and apparatus for magnetically separating selected particles, particularly biological cells
PCT/IL1999/000255 WO1999059694A1 (en) 1998-05-17 1999-05-13 Method and apparatus for magnetically separating selected particles, particularly biological cells

Publications (2)

Publication Number Publication Date
BR9911023A true BR9911023A (pt) 2001-09-25
BR9911023B1 BR9911023B1 (pt) 2009-12-01

Family

ID=11071507

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9911023-7A BR9911023B1 (pt) 1998-05-17 1999-05-13 processo e aparelho para separar magneticamente partìculas selecionadas.

Country Status (14)

Country Link
US (2) US6482328B1 (pt)
EP (1) EP1093393B1 (pt)
JP (1) JP4713735B2 (pt)
AT (1) ATE399048T1 (pt)
AU (1) AU754781B2 (pt)
BR (1) BR9911023B1 (pt)
CA (1) CA2333299C (pt)
DE (1) DE69938968D1 (pt)
DK (1) DK1093393T3 (pt)
ES (1) ES2310037T3 (pt)
HK (1) HK1037151A1 (pt)
IL (1) IL124514A (pt)
MX (1) MXPA00011129A (pt)
WO (1) WO1999059694A1 (pt)

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US8211386B2 (en) 2004-06-08 2012-07-03 Biokit, S.A. Tapered cuvette and method of collecting magnetic particles
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US8303894B2 (en) 2005-10-13 2012-11-06 Accuri Cytometers, Inc. Detection and fluidic system of a flow cytometer
US8017402B2 (en) 2006-03-08 2011-09-13 Accuri Cytometers, Inc. Fluidic system for a flow cytometer
US7776268B2 (en) * 2005-10-13 2010-08-17 Accuri Cytometers, Inc. User interface for a fluidic system of a flow cytometer
US7857005B2 (en) * 2005-12-07 2010-12-28 Accuri Cytometers, Inc. Pulsation attenuator for a fluidic system
US8273311B2 (en) * 2006-01-11 2012-09-25 Korea Institute Of Science And Technology Apparatus and method for separating biological particles using difference between gravity and magnetic force
US7780916B2 (en) 2006-03-08 2010-08-24 Accuri Cytometers, Inc. Flow cytometer system with unclogging feature
US8283177B2 (en) * 2006-03-08 2012-10-09 Accuri Cytometers, Inc. Fluidic system with washing capabilities for a flow cytometer
US7981661B2 (en) * 2006-04-17 2011-07-19 Accuri Cytometers, Inc. Flow cytometer system with sheath and waste fluid measurement
US8715573B2 (en) * 2006-10-13 2014-05-06 Accuri Cytometers, Inc. Fluidic system for a flow cytometer with temporal processing
KR100815858B1 (ko) * 2006-10-16 2008-03-24 셀바이오주식회사 생체물질의 분리장치 및 방법
US8445286B2 (en) 2006-11-07 2013-05-21 Accuri Cytometers, Inc. Flow cell for a flow cytometer system
US7739060B2 (en) * 2006-12-22 2010-06-15 Accuri Cytometers, Inc. Detection system and user interface for a flow cytometer system
US8432541B2 (en) * 2007-12-17 2013-04-30 Accuri Cytometers, Inc. Optical system for a flow cytometer with an interrogation zone
US7947492B2 (en) * 2008-08-20 2011-05-24 Northeastern Ohio Universities College Of Medicine Device improving the detection of a ligand
JP2010207133A (ja) 2009-03-10 2010-09-24 Sony Corp 細胞分離方法
US20110061471A1 (en) * 2009-06-02 2011-03-17 Rich Collin A System and method of verification of a sample for a flow cytometer
US8507279B2 (en) 2009-06-02 2013-08-13 Accuri Cytometers, Inc. System and method of verification of a prepared sample for a flow cytometer
JP5846609B2 (ja) * 2010-01-21 2016-01-20 バイオセップ リミテッド 希少細胞の磁気分離
US8779387B2 (en) 2010-02-23 2014-07-15 Accuri Cytometers, Inc. Method and system for detecting fluorochromes in a flow cytometer
US9551600B2 (en) 2010-06-14 2017-01-24 Accuri Cytometers, Inc. System and method for creating a flow cytometer network
CN103168225B (zh) 2010-10-25 2015-11-25 阿库里赛托梅特斯公司 用于收集流式细胞仪中的数据集的***和用户接口
US8528427B2 (en) 2010-10-29 2013-09-10 Becton, Dickinson And Company Dual feedback vacuum fluidics for a flow-type particle analyzer
CN104471371B (zh) * 2011-12-21 2018-11-09 贝克顿·迪金森公司 用于对磁标记的样品组分进行无菌分离的流式细胞计数***
US9517474B2 (en) 2012-05-18 2016-12-13 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles
CN103773682B (zh) * 2014-01-23 2015-09-30 张利峰 细胞磁分选***、分选装置和处理设备
US20150284862A1 (en) * 2014-04-07 2015-10-08 John Staley Brookshire Continuous Flow Process for Producing Storable Consumable Magnascent Iodine
CN103949341A (zh) * 2014-04-29 2014-07-30 东南大学 一种全自动生物样本处理装置
GB2598499B (en) * 2015-06-05 2022-05-25 Novartis Ag Flow-through paramagnetic particle-based cell separation and paramagnetic particle removal
US10676719B2 (en) 2015-07-31 2020-06-09 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles
US10189029B2 (en) * 2016-06-30 2019-01-29 United Arab Emirates University Magnetic particle separator
JP2020501873A (ja) * 2016-10-31 2020-01-23 アムジエン・インコーポレーテツド 精製システム及び方法
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Also Published As

Publication number Publication date
AU3845299A (en) 1999-12-06
IL124514A0 (en) 1998-12-06
DK1093393T3 (da) 2008-10-06
EP1093393A1 (en) 2001-04-25
ATE399048T1 (de) 2008-07-15
MXPA00011129A (es) 2002-08-06
HK1037151A1 (en) 2002-02-01
DE69938968D1 (de) 2008-08-07
US20030062314A1 (en) 2003-04-03
EP1093393A4 (en) 2002-09-11
CA2333299C (en) 2007-09-18
BR9911023B1 (pt) 2009-12-01
WO1999059694A1 (en) 1999-11-25
ES2310037T3 (es) 2008-12-16
IL124514A (en) 2002-02-10
JP2002515319A (ja) 2002-05-28
US6482328B1 (en) 2002-11-19
JP4713735B2 (ja) 2011-06-29
EP1093393B1 (en) 2008-06-25
AU754781B2 (en) 2002-11-28
CA2333299A1 (en) 1999-11-25

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B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]
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B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)