BR9712532A - Método para aumentar o nìvel de expressão de um transgene população de células monocotiledÈneas,plantas e sementes - Google Patents

Método para aumentar o nìvel de expressão de um transgene população de células monocotiledÈneas,plantas e sementes

Info

Publication number
BR9712532A
BR9712532A BR9712532-6A BR9712532A BR9712532A BR 9712532 A BR9712532 A BR 9712532A BR 9712532 A BR9712532 A BR 9712532A BR 9712532 A BR9712532 A BR 9712532A
Authority
BR
Brazil
Prior art keywords
population
expression
plants
transgene
seeds
Prior art date
Application number
BR9712532-6A
Other languages
English (en)
Inventor
Joan Tellefsen Odell
Enno Krebbers
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9712532A publication Critical patent/BR9712532A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

<B>"MéTODO PARA AUMENTAR O NìVEL DE EXPRESSãO DE UM TRANSGENE, POPULAçãO DE CéLULAS MONOCOTILEDÈNEAS, PLANTAS E SEMENTES"<D>. A presente invenção refere-se a um método de aumentar a expressão de transgenes em uma população de plantas monocotiledóneas, o qual envolve o emprego de uma construção de DNA que compreende, entre outras, pelo menos uma região de ligação do tipo plataforma do local de gene de lisozima de galinha.
BR9712532-6A 1996-10-17 1997-10-01 Método para aumentar o nìvel de expressão de um transgene população de células monocotiledÈneas,plantas e sementes BR9712532A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2816596P 1996-10-17 1996-10-17
PCT/US1997/017709 WO1998016650A1 (en) 1996-10-17 1997-10-01 Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions

Publications (1)

Publication Number Publication Date
BR9712532A true BR9712532A (pt) 1999-10-19

Family

ID=21841936

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9712532-6A BR9712532A (pt) 1996-10-17 1997-10-01 Método para aumentar o nìvel de expressão de um transgene população de células monocotiledÈneas,plantas e sementes

Country Status (12)

Country Link
EP (1) EP0931155A1 (pt)
JP (1) JP2000504943A (pt)
KR (1) KR20000049209A (pt)
AR (1) AR013853A1 (pt)
AU (1) AU4893397A (pt)
BR (1) BR9712532A (pt)
CA (1) CA2263891A1 (pt)
HU (1) HUP0000064A3 (pt)
IL (1) IL129268A0 (pt)
PL (1) PL332938A1 (pt)
WO (1) WO1998016650A1 (pt)
ZA (1) ZA979226B (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2292842A1 (en) * 1997-06-03 1998-12-10 North Carolina State University Method for reducing expression variability of transgenes in plants cells
AU3476399A (en) * 1998-07-31 2000-02-21 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
CN1127570C (zh) * 1998-11-20 2003-11-12 中国科学院上海生物化学研究所 蓖麻蚕细胞核骨架结合元件的应用
DE19857654A1 (de) 1998-12-14 2000-06-15 Max Planck Gesellschaft Beeinflussung des Blühverhaltens von Pflanzen durch Expression Saccharose-spaltender Proteine
US6608242B1 (en) 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants
US6743969B2 (en) 2000-11-14 2004-06-01 E. I. Du Pont De Nemours And Company Modification of PI-TA gene conferring fungal disease resistance to plants
ATE446364T1 (de) 2001-03-23 2009-11-15 Du Pont Wurzelspezifischer, durch stimulantien induzierbarer promotor und seine verwendung
DK1513871T3 (da) 2002-02-04 2009-02-23 Isis Innovation Pyrrolidinyl peptid nucleinsyrer
CN100480261C (zh) * 2003-02-26 2009-04-22 中国科学院上海生命科学研究院 核基质蛋白结合序列及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
ATE217319T1 (de) * 1992-10-05 2002-05-15 Univ North Carolina State Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
AU6679094A (en) * 1993-04-19 1994-11-08 Sandoz Ltd. Genetic stabilizing elements

Also Published As

Publication number Publication date
WO1998016650A1 (en) 1998-04-23
HUP0000064A3 (en) 2002-02-28
IL129268A0 (en) 2000-02-17
CA2263891A1 (en) 1998-04-23
AU4893397A (en) 1998-05-11
KR20000049209A (ko) 2000-07-25
JP2000504943A (ja) 2000-04-25
PL332938A1 (en) 1999-10-25
EP0931155A1 (en) 1999-07-28
HUP0000064A2 (hu) 2000-05-28
ZA979226B (en) 1999-04-15
AR013853A1 (es) 2001-01-31

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Legal Events

Date Code Title Description
FB34 Technical and formal requirements: requirement - article 34 of industrial property law
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]