BR9609241A - Moléculas de avidina e estreptavidina modificadas e uso das mesmas. - Google Patents
Moléculas de avidina e estreptavidina modificadas e uso das mesmas.Info
- Publication number
- BR9609241A BR9609241A BR9609241-6A BR9609241A BR9609241A BR 9609241 A BR9609241 A BR 9609241A BR 9609241 A BR9609241 A BR 9609241A BR 9609241 A BR9609241 A BR 9609241A
- Authority
- BR
- Brazil
- Prior art keywords
- avidin
- modified
- molecules
- streptavidin
- tyrosine residue
- Prior art date
Links
- 108090001008 Avidin Proteins 0.000 title abstract 8
- 108010090804 Streptavidin Proteins 0.000 title abstract 4
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract 3
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 abstract 3
- 229960002685 biotin Drugs 0.000 abstract 2
- 235000020958 biotin Nutrition 0.000 abstract 2
- 239000011616 biotin Substances 0.000 abstract 2
- 125000000751 azo group Chemical group [*]N=N[*] 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 125000005843 halogen group Chemical group 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/77—Ovalbumin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Peptides Or Proteins (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Abstract
Patente de Invenção: <B>''MOLéCULAS DE AVIDINA E ESTREPTAVIDINA MODIFICADAS E USO DAS MESMAS''<D>. São fornecidos moléculas do tipo de avidina modificada com ligação de biotina nas quais o resídio de tirosina essencial dentro do sítio de ligação de biotona é modificado em um tal modo que seu pKa é diminuído em comparação com o pka do resíduo de tirosina não-modificado na molécula do tipo de avidina não-modificada correspondente. As moléculas do tipo da avidina incluem: (i) avidina da clara do ovo natural; (ii) avidina recombinante ; (iii) formas desglicodiladas de avidina; (iv) estreptavidina bacteriana; (v) estreptadivina recombinante; (vi) estreptavidina truncada; e (vii) derivados de (i) - (Vi) os quais são modificados em sítios diferentes do resíduo de tirosina essencial. A modificação é obtida através da substituição em uma ou ambas as posições orto para os radicais hidróxi de tirosina através de radicais tais como nitro, halogeno, azo e amino. As moléculas do tipo de avidina modificada podem ser usadas em todas as aplicações da tecnologia de avidina - biotina.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL11414995A IL114149A0 (en) | 1995-06-14 | 1995-06-14 | Modified avidin and streptavidin molecules and use thereof |
PCT/IL1996/000014 WO1997000329A2 (en) | 1995-06-14 | 1996-06-13 | Modified avidin and streptavidin molecules and use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9609241A true BR9609241A (pt) | 1999-10-13 |
Family
ID=11067612
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9609241-6A BR9609241A (pt) | 1995-06-14 | 1996-06-13 | Moléculas de avidina e estreptavidina modificadas e uso das mesmas. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5973124A (pt) |
EP (1) | EP0871658B1 (pt) |
JP (1) | JPH11507920A (pt) |
KR (1) | KR19990022896A (pt) |
CN (1) | CN1119353C (pt) |
AT (1) | ATE481417T1 (pt) |
AU (1) | AU713417B2 (pt) |
BR (1) | BR9609241A (pt) |
CA (1) | CA2221647A1 (pt) |
CZ (1) | CZ401697A3 (pt) |
DE (1) | DE69638257D1 (pt) |
HU (1) | HUP9900239A3 (pt) |
IL (1) | IL114149A0 (pt) |
WO (1) | WO1997000329A2 (pt) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19637718A1 (de) * | 1996-04-01 | 1997-10-02 | Boehringer Mannheim Gmbh | Rekombinante inaktive Core-Streptavidin Mutanten |
US6368813B1 (en) | 1997-03-14 | 2002-04-09 | The Trustees Of Boston University | Multiflavor streptavidin |
US6391571B1 (en) | 1997-04-01 | 2002-05-21 | Roche Diagnostics Gmbh | Recombinant inactive avidin mutants |
US5985658A (en) * | 1997-11-14 | 1999-11-16 | Health Research Incorporated | Calmodulin-based cell separation technique |
WO2000028326A1 (fr) * | 1998-11-06 | 2000-05-18 | Iatron Laboratories, Inc. | Nouveaux complexes contenant de l'avidine reticulee, procede analytique dans lequel on utilise de l'avidine reticulee, reactifs et kits d'analyse |
IL126991A0 (en) | 1998-11-10 | 1999-09-22 | Yeda Res & Dev | Azobenzene derivatives and intermediates thereof |
WO2000027814A1 (en) * | 1998-11-10 | 2000-05-18 | Yeda Research And Development Co. Ltd. | Avidin derivatives and uses thereof |
JP2002541818A (ja) * | 1999-04-14 | 2002-12-10 | ザ ガヴァメント オブ ザ ユナイテッド ステイツ オブ アメリカ、アズ レプリゼンテッド バイ ザ セクレタリー、 デパートメント オブ ヘルス アンド ヒューマン サーヴィスィズ | 高感度ファージ提示生体分子検出 |
WO2001009141A1 (fr) * | 1999-07-29 | 2001-02-08 | National Institute Of Advanced Industrial Science And Technology | Derives de biotine polymerisables, polymere de la biotine et polymere reagissant a la stimulation de l'avidine |
JP2004501110A (ja) * | 2000-05-23 | 2004-01-15 | ネクセル セラピューティックス インコーポレイティド | 細胞選択用試薬および使用方法 |
GB0102568D0 (en) | 2001-02-01 | 2001-03-21 | Magnetic Biosolutions Sweden A | Method |
US7754155B2 (en) * | 2002-03-15 | 2010-07-13 | Ross Amelia A | Devices and methods for isolating target cells |
US20030175818A1 (en) * | 2002-03-15 | 2003-09-18 | Ross Amelia A. | Devices and methods for isolating and recovering target cells |
US9302242B2 (en) * | 2004-04-28 | 2016-04-05 | University Of Houston System | Preparation of nanometric arrays of biomolecules on oligo- or poly(ethylene glycol) films on silicon surfaces |
US8426028B2 (en) * | 2004-04-28 | 2013-04-23 | University Of Houston | Preparation of sensors on oligo- or poly (ethylene glycol) films on silicon surfaces |
WO2006086019A2 (en) * | 2004-10-21 | 2006-08-17 | Optiscan Biomedical Corporation | Methods of treating diabetes |
WO2006084388A1 (en) * | 2005-02-11 | 2006-08-17 | Uti Limited Partnership | Monomeric streptavidin muteins |
US20070025918A1 (en) * | 2005-07-28 | 2007-02-01 | General Electric Company | Magnetic resonance imaging (MRI) agents: water soluble carbon-13 enriched fullerene and carbon nanotubes for use with dynamic nuclear polarization |
US7704708B2 (en) * | 2006-02-13 | 2010-04-27 | Uti Limited Partnership | Monomeric streptavidin muteins |
EP2038320B1 (en) | 2006-06-29 | 2014-03-12 | Life Technologies AS | Particles containing multi- block polymers |
CN101622340A (zh) * | 2006-11-15 | 2010-01-06 | 茵维特罗根戴纳股份公司 | 生物素化合物与支持物可逆结合的方法 |
US8506993B2 (en) * | 2007-08-14 | 2013-08-13 | University Of Houston | Compositions and methods for making and using multifunctional polymerized liposomes |
US7842475B2 (en) * | 2008-01-08 | 2010-11-30 | Siemens Healthcare Diagnostics Inc. | Stabilization of solid support assay reagents |
US20090209447A1 (en) * | 2008-02-15 | 2009-08-20 | Michelle Meek | Cleaning compositions |
TWI365185B (en) * | 2008-07-24 | 2012-06-01 | Lilly Co Eli | Amidophenoxyindazoles useful as inhibitors of c-met |
DE102008053892A1 (de) | 2008-10-30 | 2010-05-06 | Fachhochschule Gelsenkirchen | Medizinisches Implantat mit biofunktionalisierter Oberfläche |
EP3462174B1 (en) | 2013-03-15 | 2021-12-29 | Arizona Board of Regents on behalf of Arizona State University | Biosensor microarray compositions and methods |
CN105308437B (zh) | 2013-03-15 | 2018-01-19 | Hycor 生物医学有限责任公司 | 进行样本的冷光和荧光测量的装置和相关方法 |
WO2015195404A1 (en) | 2014-06-20 | 2015-12-23 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Methods for detection of an analyte by movement of tethered microparticles |
WO2016205239A1 (en) | 2015-06-15 | 2016-12-22 | The United States Of America, As Represented By The Secretary Department Of Health And Human Services | Non-hormonal mammalian sperm decoy contraception based on the n-terminus of the zp2 protein |
CA3041059C (en) | 2016-10-21 | 2023-09-26 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Molecular nanotags |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3027770B2 (ja) * | 1994-03-05 | 2000-04-04 | ロシュ ダイアグノスティックス ゲーエムベーハー | イムノアッセイで使用するための干渉除去剤 |
-
1995
- 1995-06-14 IL IL11414995A patent/IL114149A0/xx unknown
-
1996
- 1996-06-13 HU HU9900239A patent/HUP9900239A3/hu unknown
- 1996-06-13 CN CN96195475A patent/CN1119353C/zh not_active Expired - Fee Related
- 1996-06-13 AT AT96918819T patent/ATE481417T1/de not_active IP Right Cessation
- 1996-06-13 CA CA002221647A patent/CA2221647A1/en not_active Abandoned
- 1996-06-13 JP JP9502873A patent/JPH11507920A/ja not_active Ceased
- 1996-06-13 EP EP96918819A patent/EP0871658B1/en not_active Expired - Lifetime
- 1996-06-13 BR BR9609241-6A patent/BR9609241A/pt not_active Application Discontinuation
- 1996-06-13 CZ CZ974016A patent/CZ401697A3/cs unknown
- 1996-06-13 AU AU61353/96A patent/AU713417B2/en not_active Ceased
- 1996-06-13 WO PCT/IL1996/000014 patent/WO1997000329A2/en not_active Application Discontinuation
- 1996-06-13 KR KR1019970709365A patent/KR19990022896A/ko not_active Application Discontinuation
- 1996-06-13 DE DE69638257T patent/DE69638257D1/de not_active Expired - Lifetime
- 1996-06-13 US US08/973,970 patent/US5973124A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69638257D1 (de) | 2010-10-28 |
HUP9900239A2 (hu) | 1999-05-28 |
ATE481417T1 (de) | 2010-10-15 |
AU6135396A (en) | 1997-01-15 |
WO1997000329A3 (en) | 1997-03-06 |
CN1191018A (zh) | 1998-08-19 |
KR19990022896A (ko) | 1999-03-25 |
HUP9900239A3 (en) | 1999-11-29 |
EP0871658B1 (en) | 2010-09-15 |
JPH11507920A (ja) | 1999-07-13 |
CA2221647A1 (en) | 1997-01-03 |
US5973124A (en) | 1999-10-26 |
MX9710181A (es) | 1998-03-31 |
EP0871658A4 (en) | 2002-12-04 |
EP0871658A2 (en) | 1998-10-21 |
CN1119353C (zh) | 2003-08-27 |
AU713417B2 (en) | 1999-12-02 |
WO1997000329A2 (en) | 1997-01-03 |
CZ401697A3 (cs) | 1998-07-15 |
IL114149A0 (en) | 1995-10-31 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FB36 | Technical and formal requirements: requirement - article 36 of industrial property law | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |