BR9508053A - Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga - Google Patents

Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga

Info

Publication number
BR9508053A
BR9508053A BR9508053A BR9508053A BR9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A BR 9508053 A BR9508053 A BR 9508053A
Authority
BR
Brazil
Prior art keywords
construction
production
heterologous protein
yeast strain
vector dna
Prior art date
Application number
BR9508053A
Other languages
English (en)
Inventor
Thomas Borglum Kjeldsen
Knud Vad
Jokob Brandt
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9508053A publication Critical patent/BR9508053A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
BR9508053A 1994-06-17 1995-06-16 Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga BR9508053A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17
PCT/DK1995/000250 WO1995035384A1 (en) 1994-06-17 1995-06-16 N-terminally extended proteins expressed in yeast

Publications (1)

Publication Number Publication Date
BR9508053A true BR9508053A (pt) 1997-08-12

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9508053A BR9508053A (pt) 1994-06-17 1995-06-16 Construção de DNA vetor de expressão recombinante cepa de levedura e processo para a produção de uma proteina heteróloga

Country Status (16)

Country Link
EP (1) EP0765395A1 (pt)
JP (1) JP3730255B2 (pt)
CN (1) CN1131312C (pt)
AU (1) AU2733595A (pt)
BR (1) BR9508053A (pt)
CA (1) CA2192943A1 (pt)
CZ (1) CZ363796A3 (pt)
FI (1) FI965030A (pt)
HU (1) HUT75840A (pt)
IL (1) IL114160A (pt)
MX (1) MX9606536A (pt)
NO (1) NO965411L (pt)
PL (1) PL317761A1 (pt)
SG (1) SG47883A1 (pt)
WO (1) WO1995035384A1 (pt)
ZA (1) ZA954983B (pt)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
ATE398139T1 (de) * 1996-12-13 2008-07-15 Novartis Vaccines & Diagnostic Analyse und separation von pdgf-proteinen
DE69735242T2 (de) * 1996-12-20 2006-09-21 Novo Nordisk A/S N-terminal verlängerte proteine exprimiert in hefe
EP1049790A1 (en) 1998-01-23 2000-11-08 Novo Nordisk A/S Process for making desired polypeptides in yeast
WO2001049870A1 (en) 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
JP2005503141A (ja) * 2001-07-24 2005-02-03 ノボ　ノルディスク　アクティーゼルスカブ アシル化ポリペプチドを作出するための方法
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
EP1692168B1 (en) 2003-12-03 2011-07-20 Novo Nordisk A/S Single-chain insulin
WO2007020256A1 (en) 2005-08-16 2007-02-22 Novo Nordisk A/S Method for making mature insulin polypeptides
JP5550338B2 (ja) 2006-07-31 2014-07-16 ノボ・ノルデイスク・エー／エス ペグ化持続型インスリン
US9018161B2 (en) 2006-09-22 2015-04-28 Novo Nordisk A/S Protease resistant insulin analogues
WO2008037735A1 (en) 2006-09-27 2008-04-03 Novo Nordisk A/S Method for making maturated insulin polypeptides
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
CN101743252A (zh) 2007-07-16 2010-06-16 诺沃-诺迪斯克有限公司 蛋白酶稳定化的、peg化的胰岛素类似物
WO2009021955A1 (en) 2007-08-13 2009-02-19 Novo Nordisk A/S Rapid acting insulin analogues
ES2548304T3 (es) 2007-08-15 2015-10-15 Novo Nordisk A/S Análogos de la insulina que contienen una fracción acilo y alquilenglicol
ES2526924T3 (es) 2007-08-15 2015-01-16 Novo Nordisk A/S Insulinas con una fracción acilo que comprende unidades repetitivas de aminoácidos que contienen alquilenglicol
PL2254906T3 (pl) 2008-03-18 2017-04-28 Novo Nordisk A/S Stabilizowane względem proteaz, acylowane analogi insuliny
CN102065903B (zh) 2008-04-01 2015-02-18 诺沃-诺迪斯克有限公司 胰岛素清蛋白缀合物
DK2340033T3 (da) 2009-06-26 2014-01-20 Novo Nordisk As Præparat, der omfatter insulin, nicotinamid og arginin
CN102639559B (zh) 2009-11-25 2015-04-29 诺沃—诺迪斯克有限公司 用于制备多肽的方法
CA2802510A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin derivatives containing additional disulfide bonds
EP2585483A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Human insulin containing additional disulfide bonds
EP2585484A1 (en) 2010-06-23 2013-05-01 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
KR20140030125A (ko) 2010-12-14 2014-03-11 노보 노르디스크 에이/에스 인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
JP2013545782A (ja) 2010-12-14 2013-12-26 ノヴォ ノルディスク アー／エス 長時間作用型インスリンと組み合わせた速効型インスリン
WO2012171994A1 (en) 2011-06-15 2012-12-20 Novo Nordisk A/S Multi substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
US9707276B2 (en) 2012-12-03 2017-07-18 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (CTP) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
WO2015128507A1 (en) 2014-02-28 2015-09-03 Novo Nordisk A/S Mating factor alpha pro-peptide variants
EP3303380B1 (en) 2015-06-02 2020-01-15 Novo Nordisk A/S Insulins with polar recombinant extensions
JP2018531899A (ja) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス 新規インスリン誘導体及びその医学的使用
ES2908373T3 (es) 2015-08-25 2022-04-28 Novo Nordisk As Nuevos derivados de insulina y los usos médicos de los mismos
US20190010206A1 (en) 2015-08-25 2019-01-10 Novo Nordisk A/S Novel Insulin Derivatives and the Medical Uses Hereof
MX2018014023A (es) * 2016-05-24 2019-04-04 Novo Nordisk As Compuestos de citocina inhibidora de macrofagos 1 (mic-1) y uso de estos.
EP3668892A1 (en) 2017-08-17 2020-06-24 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
JP2021532730A (ja) * 2018-06-18 2021-12-02 ユニケム ラボラトリーズ リミテッド 組換えタンパク質の発現レベルを高めるためのリーダー配列
JP6995284B2 (ja) 2019-12-11 2022-01-14 ノヴォ ノルディスク アー／エス 新規インスリン類似体およびそれらの使用
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
AU2733595A (en) 1996-01-15
ZA954983B (en) 1996-02-14
IL114160A (en) 2006-12-31
WO1995035384A1 (en) 1995-12-28
NO965411L (no) 1997-02-14
FI965030A0 (fi) 1996-12-16
IL114160A0 (en) 1995-10-31
CA2192943A1 (en) 1995-12-28
SG47883A1 (en) 1998-04-17
NO965411D0 (no) 1996-12-16
CZ363796A3 (en) 1997-05-14
FI965030A (fi) 1997-02-14
JP3730255B2 (ja) 2005-12-21
JPH10501695A (ja) 1998-02-17
HUT75840A (en) 1997-05-28
CN1152942A (zh) 1997-06-25
EP0765395A1 (en) 1997-04-02
PL317761A1 (en) 1997-04-28
HU9603476D0 (en) 1997-02-28
MX9606536A (es) 1997-03-29
CN1131312C (zh) 2003-12-17

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law