SG47883A1 - N-terminally extended proteins expressed in yeast - Google Patents

N-terminally extended proteins expressed in yeast

Info

Publication number
SG47883A1
SG47883A1 SG1996004970A SG1996004970A SG47883A1 SG 47883 A1 SG47883 A1 SG 47883A1 SG 1996004970 A SG1996004970 A SG 1996004970A SG 1996004970 A SG1996004970 A SG 1996004970A SG 47883 A1 SG47883 A1 SG 47883A1
Authority
SG
Singapore
Prior art keywords
yeast
proteins expressed
terminally extended
extended proteins
terminally
Prior art date
Application number
SG1996004970A
Inventor
Knud Vad
Jakob Brandt
Thomas Borglum Kjeldsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of SG47883A1 publication Critical patent/SG47883A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
SG1996004970A 1994-06-17 1995-09-16 N-terminally extended proteins expressed in yeast SG47883A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK71294 1994-06-17

Publications (1)

Publication Number Publication Date
SG47883A1 true SG47883A1 (en) 1998-04-17

Family

ID=8096676

Family Applications (1)

Application Number Title Priority Date Filing Date
SG1996004970A SG47883A1 (en) 1994-06-17 1995-09-16 N-terminally extended proteins expressed in yeast

Country Status (16)

Country Link
EP (1) EP0765395A1 (en)
JP (1) JP3730255B2 (en)
CN (1) CN1131312C (en)
AU (1) AU2733595A (en)
BR (1) BR9508053A (en)
CA (1) CA2192943A1 (en)
CZ (1) CZ363796A3 (en)
FI (1) FI965030A (en)
HU (1) HUT75840A (en)
IL (1) IL114160A (en)
MX (1) MX9606536A (en)
NO (1) NO965411L (en)
PL (1) PL317761A1 (en)
SG (1) SG47883A1 (en)
WO (1) WO1995035384A1 (en)
ZA (1) ZA954983B (en)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
DE69739319D1 (en) 1996-12-13 2009-04-30 Novartis Vaccines & Diagnostic Method for expression of PDGF or IGR proteins in yeast
ES2258797T3 (en) * 1996-12-20 2006-09-01 Novo Nordisk A/S PROTEINS WITH N-TERMINAL EXTENSION EXPRESSED IN LEAVING.
JP2003525570A (en) * 1998-01-23 2003-09-02 ノボ ノルディスク アクティーゼルスカブ Method for producing desired polypeptide in yeast
DE60044728D1 (en) 1999-12-29 2010-09-02 Novo Nordisk As METHOD FOR THE PRODUCTION OF INSULIN FORECASTS AND ANALOGS OF INSULIN PROCESSORS WITH IMPROVED FERMENTATION EXPORTS IN YEAST
AU2002355160A1 (en) * 2001-07-24 2003-02-17 Novo Nordisk A/S Method for making acylated polypeptides
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
US20030082671A1 (en) 2001-07-24 2003-05-01 Thomas Hoeg-Jensen Method for making acylated polypeptides
WO2004085472A1 (en) * 2003-03-27 2004-10-07 Novo Nordisk A/S Method for making human insulin precursors and human insulin
ES2369895T3 (en) 2003-12-03 2011-12-07 Novo Nordisk A/S MONOCATENARY INSULIN.
CN102660614A (en) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 Method for making mature insulin polypeptides
ES2542146T3 (en) * 2006-07-31 2015-07-31 Novo Nordisk A/S PEGylated extended insulin.
KR101699370B1 (en) 2006-09-22 2017-02-14 노보 노르디스크 에이/에스 Protease resistant insulin analogues
EP2069502B1 (en) 2006-09-27 2014-02-26 Novo Nordisk A/S Method for making maturated insulin polypeptides
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
CN101743252A (en) 2007-07-16 2010-06-16 诺沃-诺迪斯克有限公司 protease stabilized, pegylated insulin analogues
WO2009021955A1 (en) 2007-08-13 2009-02-19 Novo Nordisk A/S Rapid acting insulin analogues
ES2526924T3 (en) 2007-08-15 2015-01-16 Novo Nordisk A/S Insulins with an acyl fraction comprising repetitive amino acid units containing alkylene glycol
EP2178912B1 (en) 2007-08-15 2015-07-08 Novo Nordisk A/S Insulin analogues with an acyl and aklylene glycol moiety
WO2009115469A1 (en) 2008-03-18 2009-09-24 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
ES2563553T3 (en) 2008-04-01 2016-03-15 Novo Nordisk A/S Insulin-albumin conjugates
CA2764423A1 (en) 2009-06-26 2010-12-29 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
EP2504355B1 (en) 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
WO2011161124A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin analogues containing additional disulfide bonds
US8883722B2 (en) 2010-06-23 2014-11-11 Novo Nordisk A/S Human insulin containing additional disulfide bonds
CA2802510A1 (en) 2010-06-23 2011-12-29 Novo Nordisk A/S Insulin derivatives containing additional disulfide bonds
US20130331320A1 (en) 2010-12-14 2013-12-12 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
MX2013006174A (en) 2010-12-14 2013-07-15 Novo Nordisk As Preparation comprising insulin, nicotinamide and an amino acid.
WO2012171994A1 (en) 2011-06-15 2012-12-20 Novo Nordisk A/S Multi substituted insulins
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
CA2890048C (en) 2012-12-03 2022-05-03 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
JP6983075B2 (en) 2015-06-02 2021-12-17 ノヴォ ノルディスク アー／エス Insulin with polar recombination extension
JP2018531900A (en) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス Novel insulin derivatives and their medical use
JP2018531899A (en) 2015-08-25 2018-11-01 ノヴォ ノルディスク アー／エス Novel insulin derivatives and their medical use
CN108026156A (en) 2015-08-25 2018-05-11 诺和诺德股份有限公司 Novel insulin derivates and its medical usage
BR112018072034A2 (en) * 2016-05-24 2019-02-12 Novo Nordisk A/S mic-1 compounds and uses thereof
EP3668892A1 (en) 2017-08-17 2020-06-24 Novo Nordisk A/S Novel acylated insulin analogues and uses thereof
EP3807306A4 (en) * 2018-06-18 2022-04-06 Unichem Laboratories Limited Leader sequence for higher expression of recombinant proteins
CN114787184A (en) 2019-12-11 2022-07-22 诺和诺德股份有限公司 Novel insulin analogs and uses thereof
WO2023144240A1 (en) 2022-01-26 2023-08-03 Novo Nordisk Research Centre Oxford Limited Glucose sensitive insulin derivatives and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK105489D0 (en) * 1989-03-03 1989-03-03 Novo Nordisk As POLYPEPTIDE
DK300090D0 (en) * 1990-12-19 1990-12-19 Novo Nordisk As PROCEDURE FOR PREPARING LEADER SEQUENCES
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells

Also Published As

Publication number Publication date
PL317761A1 (en) 1997-04-28
FI965030A0 (en) 1996-12-16
CA2192943A1 (en) 1995-12-28
FI965030A (en) 1997-02-14
CN1152942A (en) 1997-06-25
JPH10501695A (en) 1998-02-17
HUT75840A (en) 1997-05-28
ZA954983B (en) 1996-02-14
IL114160A (en) 2006-12-31
MX9606536A (en) 1997-03-29
HU9603476D0 (en) 1997-02-28
JP3730255B2 (en) 2005-12-21
CZ363796A3 (en) 1997-05-14
CN1131312C (en) 2003-12-17
NO965411D0 (en) 1996-12-16
WO1995035384A1 (en) 1995-12-28
IL114160A0 (en) 1995-10-31
AU2733595A (en) 1996-01-15
EP0765395A1 (en) 1997-04-02
NO965411L (en) 1997-02-14
BR9508053A (en) 1997-08-12

Similar Documents

Publication Publication Date Title
SG47883A1 (en) N-terminally extended proteins expressed in yeast
AU1884797A (en) Improvements in or relating to contrast agents
AU4714797A (en) Improvements in or relating to contrast agents
AU1668795A (en) Improvements in or relating to contrast agents
GB9603069D0 (en) Improvements in or relating to gene expression
AU2316797A (en) Shc proteins
AU3902297A (en) Two human nsp-like proteins
IL133582A0 (en) Mammalian neuro-growth factor like protein
AU1677199A (en) Improvements in or relating to ultrasonography
AU1945395A (en) Use of xylanase in baking
GB9405629D0 (en) Recombinant DNA for use in yeast
GB9423372D0 (en) Proteins and their uses
IL115317A0 (en) Proteins
HUP9801330A3 (en) Novel pka-binding proteins and uses thereof
GB9413316D0 (en) Novel proteins
AU2433895A (en) Improvements in carpet making
AU3411999A (en) Novel rnase-like protein and its use
GB9402906D0 (en) Improvements in swatches
AU1617495A (en) Novel protein PHBP-70
AU9359098A (en) Improvements in or relating to chairs
AU4266197A (en) Granulocyte chemotactic protein 2 variant
GB9305786D0 (en) Recombinant dna for use in yeast
AUPM779694A0 (en) Dustmite allergen denaturant
AU706700C (en) N-terminally chemically modified protein compositions and methods
GB9424025D0 (en) Proteins