BR112023019945A2 - Métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de erros - Google Patents
Métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de errosInfo
- Publication number
- BR112023019945A2 BR112023019945A2 BR112023019945A BR112023019945A BR112023019945A2 BR 112023019945 A2 BR112023019945 A2 BR 112023019945A2 BR 112023019945 A BR112023019945 A BR 112023019945A BR 112023019945 A BR112023019945 A BR 112023019945A BR 112023019945 A2 BR112023019945 A2 BR 112023019945A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- transposon
- based technology
- unique molecular
- error correction
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000005516 engineering process Methods 0.000 title abstract 3
- 238000012937 correction Methods 0.000 title abstract 2
- 239000000463 material Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000013459 approach Methods 0.000 abstract 1
- 238000007481 next generation sequencing Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/507—Recombinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de erros. materiais e métodos para preparar bibliotecas de ácidos nucleicos para sequenciamento de próxima geração são aqui descritos. uma variedade de abordagens é descrita em relação ao uso de identificadores moleculares exclusivos com tecnologia baseada em transposon na preparação de bibliotecas de sequenciamento. além disso, são aqui descritos materiais para sequenciamento e métodos para a identificação e a correção de erros de amplificação e de sequenciamento.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163168802P | 2021-03-31 | 2021-03-31 | |
PCT/US2022/022379 WO2022212402A1 (en) | 2021-03-31 | 2022-03-29 | Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112023019945A2 true BR112023019945A2 (pt) | 2023-11-14 |
Family
ID=81653505
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112023019945A BR112023019945A2 (pt) | 2021-03-31 | 2022-03-29 | Métodos para a preparação de bibliotecas de sequenciamento por tagmentação direcional com o uso de tecnologia baseada em transposon com identificadores moleculares exclusivos para correção de erros |
Country Status (11)
Country | Link |
---|---|
US (1) | US20240026348A1 (pt) |
EP (1) | EP4314283A1 (pt) |
JP (1) | JP2024511760A (pt) |
KR (1) | KR20230164668A (pt) |
CN (1) | CN117015603A (pt) |
AU (1) | AU2022249289A1 (pt) |
BR (1) | BR112023019945A2 (pt) |
CA (1) | CA3211172A1 (pt) |
IL (1) | IL307164A (pt) |
MX (1) | MX2023011218A (pt) |
WO (1) | WO2022212402A1 (pt) |
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2022
- 2022-03-29 MX MX2023011218A patent/MX2023011218A/es unknown
- 2022-03-29 JP JP2023557365A patent/JP2024511760A/ja active Pending
- 2022-03-29 EP EP22723498.6A patent/EP4314283A1/en active Pending
- 2022-03-29 CN CN202280022273.9A patent/CN117015603A/zh active Pending
- 2022-03-29 WO PCT/US2022/022379 patent/WO2022212402A1/en active Application Filing
- 2022-03-29 IL IL307164A patent/IL307164A/en unknown
- 2022-03-29 AU AU2022249289A patent/AU2022249289A1/en active Pending
- 2022-03-29 KR KR1020237031732A patent/KR20230164668A/ko unknown
- 2022-03-29 CA CA3211172A patent/CA3211172A1/en active Pending
- 2022-03-29 BR BR112023019945A patent/BR112023019945A2/pt unknown
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2023
- 2023-09-28 US US18/476,719 patent/US20240026348A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
IL307164A (en) | 2023-11-01 |
JP2024511760A (ja) | 2024-03-15 |
US20240026348A1 (en) | 2024-01-25 |
MX2023011218A (es) | 2023-10-02 |
KR20230164668A (ko) | 2023-12-04 |
CA3211172A1 (en) | 2022-10-06 |
AU2022249289A1 (en) | 2023-08-17 |
WO2022212402A1 (en) | 2022-10-06 |
EP4314283A1 (en) | 2024-02-07 |
CN117015603A (zh) | 2023-11-07 |
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