BR112023014557A2 - Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado - Google Patents

Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado

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Publication number
BR112023014557A2
BR112023014557A2 BR112023014557A BR112023014557A BR112023014557A2 BR 112023014557 A2 BR112023014557 A2 BR 112023014557A2 BR 112023014557 A BR112023014557 A BR 112023014557A BR 112023014557 A BR112023014557 A BR 112023014557A BR 112023014557 A2 BR112023014557 A2 BR 112023014557A2
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BR
Brazil
Prior art keywords
mfs
fred
production
hmos
sialiate
Prior art date
Application number
BR112023014557A
Other languages
English (en)
Inventor
Isotta D'arrigo
Bych Kampmann Katrine
Manos Papadakis
Margit Pedersen
Original Assignee
Glycom As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2021/051479 external-priority patent/WO2021148620A1/en
Application filed by Glycom As filed Critical Glycom As
Publication of BR112023014557A2 publication Critical patent/BR112023014557A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99004—Beta-galactoside alpha-2,3-sialyltransferase (2.4.99.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/36—Neisseria

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado. a presente invenção refere-se ao campo da produção recombinante de moléculas biológicas em células geneticamente modificadas. mais particularmente, refere-se a um método para a produção recombinante de oligossacarídeos sialilados do leite humano (hmo) utilizando uma célula geneticamente modificada que expressa uma proteína da superfamília de facilitadores principais (mfs), sendo a proteína expressa fred.
BR112023014557A 2021-01-22 2022-01-21 Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado BR112023014557A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/EP2021/051479 WO2021148620A1 (en) 2020-01-23 2021-01-22 New major facilitator superfamily (mfs) protein (fred) in hmo production
EP21185379 2021-07-13
PCT/EP2022/051295 WO2022157280A1 (en) 2021-01-22 2022-01-21 New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos

Publications (1)

Publication Number Publication Date
BR112023014557A2 true BR112023014557A2 (pt) 2023-10-17

Family

ID=80168200

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023014557A BR112023014557A2 (pt) 2021-01-22 2022-01-21 Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado

Country Status (5)

Country Link
US (1) US20240102063A1 (pt)
EP (1) EP4281564A1 (pt)
JP (1) JP2024505126A (pt)
BR (1) BR112023014557A2 (pt)
WO (1) WO2022157280A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK181497B1 (en) * 2021-05-17 2024-03-12 Dsm Ip Assets Bv ENHANCING FORMATION OF THE HMOS LNT AND/OR LNnT BY MODIFYING LACTOSE IMPORT IN THE CELL

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2456292T3 (es) 2006-03-09 2014-04-21 Centre National De La Recherche Scientifique (Cnrs) Procedimiento de producción de oligosacáridos sialilados
WO2010142305A1 (en) 2009-06-08 2010-12-16 Jennewein Biotechnologie Gmbh Hmo synthesis
CA2827313C (en) 2011-02-16 2023-08-22 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria
ES2715010T3 (es) * 2014-03-31 2019-05-31 Jennewein Biotechnologie Gmbh Fermentación total de oligosacáridos
ES2968677T3 (es) 2014-06-11 2024-05-13 Glycom As Separación de 2’-O-fucosil-lactosa de caldo de fermentación
EP3191499A4 (en) 2014-09-09 2018-06-06 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
DE202015009775U1 (de) 2014-12-16 2020-02-05 Glycom A/S Abscheidung von 2'-FL aus einer Fermentationsbrühe
EP3141610A1 (en) 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
EP3426670A4 (en) 2016-03-07 2019-11-13 Glycom A/S SEPARATION OF OLIGOSACCHARIDES FROM A FERMENTATION BROTH
US11312741B2 (en) 2016-04-19 2022-04-26 Glycom A/S Separation of oligosaccharides from fermentation broth
ES2856749T3 (es) 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Proceso para la producción de oligosacáridos fucosilados
EP3658678A1 (en) * 2017-07-26 2020-06-03 Jennewein Biotechnologie GmbH Sialyltransferases and their use in producing sialylated oligosaccharides
CN111727253A (zh) 2017-12-21 2020-09-29 格礼卡姆股份公司 用于体外和体内基因表达的核酸构建体
US20190323053A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells
WO2019209241A1 (en) * 2018-04-23 2019-10-31 Dupont Nutrition Biosciences Aps Increasing export of 2' fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US20190323052A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing export of 2? fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
DE102019218979A1 (de) 2019-12-05 2021-06-10 Airbus Operations Gmbh Leichtbaustruktur für ein Fahrzeug und Luftfahrzeug
WO2021148618A1 (en) 2020-01-23 2021-07-29 Glycom A/S New major facilitator superfamily (mfs) protein (bad) in hmo production
BR112022014414A2 (pt) 2020-01-23 2022-09-13 Glycom As Produção de hmo
AU2021210607A1 (en) 2020-01-23 2022-07-14 Glycom A/S HMO production

Also Published As

Publication number Publication date
WO2022157280A1 (en) 2022-07-28
EP4281564A1 (en) 2023-11-29
JP2024505126A (ja) 2024-02-05
US20240102063A1 (en) 2024-03-28

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