BR112022011984A2 - Enzimas alfa-1,2-fucosiltransferase de conversão de lactose - Google Patents
Enzimas alfa-1,2-fucosiltransferase de conversão de lactoseInfo
- Publication number
- BR112022011984A2 BR112022011984A2 BR112022011984A BR112022011984A BR112022011984A2 BR 112022011984 A2 BR112022011984 A2 BR 112022011984A2 BR 112022011984 A BR112022011984 A BR 112022011984A BR 112022011984 A BR112022011984 A BR 112022011984A BR 112022011984 A2 BR112022011984 A2 BR 112022011984A2
- Authority
- BR
- Brazil
- Prior art keywords
- lactose
- alpha
- fucosyltransferase
- converting alpha
- fucosyltransferase enzymes
- Prior art date
Links
- 101710098620 Alpha-1,2-fucosyltransferase Proteins 0.000 title abstract 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 title abstract 3
- 239000008101 lactose Substances 0.000 title abstract 3
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 102000006471 Fucosyltransferases Human genes 0.000 abstract 1
- 108010019236 Fucosyltransferases Proteins 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01069—Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
ENZIMAS ALFA-1,2-FUCOSILTRANSFERASE DE CONVERSÃO DE LACTOSE. A presente invenção refere-se aos métodos para a produção de 2' fucosilactose (2'-FL), bem como fucosiltransferases identificadas recentemente, mais especificamente polipeptídeos de alfa-1,2-fucosiltransferase de ligação à lactose identificados recentemente e suas aplicações. Além disso, a presente invenção fornece métodos para a produção de 2-fucosilactose (2'FL) usando as alfa-1,2-fucosiltransferases identificadas recentemente.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE201905929 | 2019-12-17 | ||
PCT/EP2020/086370 WO2021122708A1 (en) | 2019-12-17 | 2020-12-16 | Lactose converting alpha-1,2-fucosyltransferase enzymes |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022011984A2 true BR112022011984A2 (pt) | 2022-08-30 |
Family
ID=69844309
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022011984A BR112022011984A2 (pt) | 2019-12-17 | 2020-12-16 | Enzimas alfa-1,2-fucosiltransferase de conversão de lactose |
Country Status (9)
Country | Link |
---|---|
US (1) | US20230348944A1 (pt) |
EP (1) | EP4077648A1 (pt) |
JP (1) | JP2023506287A (pt) |
KR (1) | KR20220116243A (pt) |
CN (1) | CN114829592A (pt) |
AU (1) | AU2020403864A1 (pt) |
BR (1) | BR112022011984A2 (pt) |
CA (1) | CA3164948A1 (pt) |
WO (1) | WO2021122708A1 (pt) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20240035055A1 (en) | 2020-08-10 | 2024-02-01 | Inbiose N.V. | Process for purification of an oligosaccharide solution produced by cell cultivation or microbial fermentation |
KR20240035566A (ko) * | 2021-07-16 | 2024-03-15 | 인바이오스 엔.브이. | 락토-n-바이오스 전환 푸코실트랜스퍼라제 |
KR20240037346A (ko) * | 2021-08-05 | 2024-03-21 | 와커 헤미 아게 | 2'-푸코실락토오스의 생체촉매 합성을 위한 특이적 알파-1,2-푸코실트랜스퍼라제 |
WO2023111140A1 (en) | 2021-12-15 | 2023-06-22 | Inbiose N.V. | Novel drying method for oligosaccharides |
KR20240002283A (ko) * | 2022-06-28 | 2024-01-05 | 주식회사 삼양사 | 2’-푸코실락토오스 제조방법 |
WO2024047096A1 (en) | 2022-08-30 | 2024-03-07 | Inbiose N.V. | Process for purification of an oligosaccharide |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ605093A (en) | 2010-07-12 | 2014-05-30 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
EP2479263B1 (en) | 2011-01-20 | 2013-11-27 | Jennewein Biotechnologie GmbH | Novel Fucosyltransferases and their applications |
US9944965B2 (en) * | 2012-12-20 | 2018-04-17 | The Board Of Trustees Of The University Of Illinois | Biosynthesis of oligosaccharides |
JP2017515455A (ja) | 2014-05-15 | 2017-06-15 | グリコシン リミテッド ライアビリティー カンパニー | フコシル化オリゴ糖の生産に使用するためのアルファ(1,2)フコシルトランスフェラーゼ・シンジーン |
KR101648342B1 (ko) * | 2014-06-23 | 2016-08-16 | 서울대학교산학협력단 | 가용성 단백질 발현량이 증대된 헬리코박터 파일로리 유래 α-1,2 푸코실 전달효소의 유전자와 단백질 및 α-1,2 푸코실올리고당 생산에의 응용 |
JP6765372B2 (ja) | 2014-11-14 | 2020-10-07 | インビオース エヌ.ヴェ.Inbiose N.V. | ラクトース死滅に耐性である変異体微生物 |
KR101731263B1 (ko) | 2016-04-25 | 2017-05-02 | 서울대학교 산학협력단 | 코리네박테리움 글루타미쿰을 이용한 2'-푸코실락토오스의 생산방법 |
EP3425052A1 (en) * | 2017-07-07 | 2019-01-09 | Jennewein Biotechnologie GmbH | Fucosyltransferases and their use in producing fucosylated oligosaccharides |
-
2020
- 2020-12-16 BR BR112022011984A patent/BR112022011984A2/pt not_active Application Discontinuation
- 2020-12-16 AU AU2020403864A patent/AU2020403864A1/en active Pending
- 2020-12-16 KR KR1020227024260A patent/KR20220116243A/ko unknown
- 2020-12-16 CN CN202080087753.4A patent/CN114829592A/zh active Pending
- 2020-12-16 US US17/785,843 patent/US20230348944A1/en active Pending
- 2020-12-16 JP JP2022537009A patent/JP2023506287A/ja active Pending
- 2020-12-16 WO PCT/EP2020/086370 patent/WO2021122708A1/en unknown
- 2020-12-16 CA CA3164948A patent/CA3164948A1/en active Pending
- 2020-12-16 EP EP20829878.6A patent/EP4077648A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP4077648A1 (en) | 2022-10-26 |
KR20220116243A (ko) | 2022-08-22 |
JP2023506287A (ja) | 2023-02-15 |
US20230348944A1 (en) | 2023-11-02 |
AU2020403864A1 (en) | 2022-08-11 |
CN114829592A (zh) | 2022-07-29 |
CA3164948A1 (en) | 2021-06-24 |
WO2021122708A1 (en) | 2021-06-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR112022011984A2 (pt) | Enzimas alfa-1,2-fucosiltransferase de conversão de lactose | |
EP4238532A3 (en) | Automatic aligner design with robust parametric optimization method | |
EP4317447A3 (en) | Donor repair templates multiplex genome editing | |
BR112017021255A2 (pt) | microrganismo modificado para a produção otimizada de 2,4-di-hidroxibutirato | |
BR112017006295A2 (pt) | microrganismos mutantes resistentes à morte por lactose | |
MX2020001903A (es) | Novedoso polipeptido y procedimiento de produccion de imp mediante el uso del mismo. | |
MY193486A (en) | Novel 5 ' -inosinic acid dehydrogenase and method preparing 5'-inosinic acid using the same | |
AU2019279282A1 (en) | Modified homoserine dehydrogenase and method for producing homoserine or l-amino acid derived from homoserine using the same | |
DOP2018000057A (es) | Composiciones biofarmacéuticas | |
MX2017011189A (es) | Dsba y dsbc ultrapurificado y métodos para elaborarlos y utilizarlos. | |
MY195583A (en) | Modified Homoserine Dehydrogenase and Method for Producing Homoserine Or L-Amino Acid Derived from Homoserine using the same | |
WO2019232025A3 (en) | Microbial engineering for the production of isoprenoids | |
DOP2015000187A (es) | Proceso de fabricación de vortioxetina | |
PH12020551948A1 (en) | Monospecific and multispecific anti-tmeff2 antibodies and their uses | |
BR112015007577A2 (pt) | métodos para cultivar sebócitos primários e identificar compostos que regulam a lipogênese e população isolada de sebócitos cultivados | |
MX2019008241A (es) | Un enlazador polipeptídico para preparar anticuerpos multispecíficos. | |
MX2021011385A (es) | Compuestos antineoplasicos. | |
MX2021015301A (es) | Métodos de cultivo celular y composiciones para la producción de anticuerpos. | |
CL2021001511A1 (es) | Novedosa psicosa-6-fosfato fosfatasa, composición para la producción de psicosa que comprende la misma y procedimiento para la producción de psicosa mediante el uso de la misma | |
WO2019004779A3 (ko) | 신규한 o-숙시닐 호모세린 트랜스퍼라제 변이체 및 이를 이용한 o-숙시닐 호모세린의 제조방법 | |
CR20220006A (es) | Compuestos para tratar enfermedad respiratoria | |
MX2022001694A (es) | Metodos y composiciones para mejorar el rendimiento de soya. | |
BR112016009089A2 (pt) | Microrganismos para a produção de o-succinil-homosserina e método para produção de o-succinil-homosserina utilizando os mesmos | |
BR112021020349A2 (pt) | Peixe estéril | |
MX2022006178A (es) | Composiciones que comprenden tricianohexano. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |