BR112019006971A2 - vetores e métodos para expressão gênica em monocotiledôneas - Google Patents

vetores e métodos para expressão gênica em monocotiledôneas

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Publication number
BR112019006971A2
BR112019006971A2 BR112019006971A BR112019006971A BR112019006971A2 BR 112019006971 A2 BR112019006971 A2 BR 112019006971A2 BR 112019006971 A BR112019006971 A BR 112019006971A BR 112019006971 A BR112019006971 A BR 112019006971A BR 112019006971 A2 BR112019006971 A2 BR 112019006971A2
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BR
Brazil
Prior art keywords
vectors
fomv
methods
expression
modified
Prior art date
Application number
BR112019006971A
Other languages
English (en)
Inventor
Bouton Clement
E Hammond-Kosack Kim
Kanyuka Konstantin
Original Assignee
Rothamsted Res Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rothamsted Res Limited filed Critical Rothamsted Res Limited
Publication of BR112019006971A2 publication Critical patent/BR112019006971A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00041—Use of virus, viral particle or viral elements as a vector
    • C12N2770/00043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

a invenção se refere ao campo de ferramentas de engenharia genética para expressão gênica em plantas. especificamente, a invenção se refere a vetores do vírus do mosaico foxtail modificados (fomv) compreendendo sequências de polinucleotídicas que são capazes de dirigir a expressão de um gene de interesse em uma planta hospedeira. consequentemente, a invenção se refere a vetores de expressão baseados em fomv compreendendo os referidos polinucleotídeos, composições compreendendo vetores de fomv modificados, métodos de geração da expressão gênica em plantas infectadas com os vetores de fomv modificados. os vetores de expressão, composições, plantas e métodos da presente invenção encontram aplicação em muitos campos da biotecnologia, incluindo, por exemplo, caracterização gênica, produção de proteína e biotecnologia agrícola.
BR112019006971A 2016-10-07 2017-10-06 vetores e métodos para expressão gênica em monocotiledôneas BR112019006971A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1617109.2A GB201617109D0 (en) 2016-10-07 2016-10-07 Vectors and methods for protein expression in plants
PCT/GB2017/053029 WO2018065785A1 (en) 2016-10-07 2017-10-06 Vectors and methods for gene expression in monocots

Publications (1)

Publication Number Publication Date
BR112019006971A2 true BR112019006971A2 (pt) 2019-10-15

Family

ID=57610637

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019006971A BR112019006971A2 (pt) 2016-10-07 2017-10-06 vetores e métodos para expressão gênica em monocotiledôneas

Country Status (6)

Country Link
US (1) US20200270621A1 (pt)
EP (1) EP3523438A1 (pt)
CN (1) CN110073001A (pt)
BR (1) BR112019006971A2 (pt)
GB (1) GB201617109D0 (pt)
WO (1) WO2018065785A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107338264B (zh) * 2016-05-03 2021-02-02 清华大学 病毒介导的基因沉默的vigs载体
CN112430619A (zh) * 2020-11-05 2021-03-02 天津师范大学 一种FoMV病毒介导的GFP-ATG8表达载体及其应用
CN112899301B (zh) * 2021-01-29 2023-04-11 中国热带农业科学院热带生物技术研究所 一种木薯普通花叶病毒诱导的基因沉默***及其应用
CN113774081B (zh) * 2021-05-11 2023-12-05 南京师范大学 一种基因编辑载体及其编辑基因的方法和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1686176A1 (en) * 2005-01-28 2006-08-02 Icon Genetics AG Production of antibodies in plants with plus-sense single-stranded viral RNA vectors
MX2010011511A (es) * 2008-04-21 2011-06-09 Danziger Innovations Ltd Vectores de expresion viral de planta y uso de los mismos para generar variaciones genotipicas en genomas de plantas.
WO2010099462A2 (en) * 2009-02-26 2010-09-02 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector

Also Published As

Publication number Publication date
GB201617109D0 (en) 2016-11-23
US20200270621A1 (en) 2020-08-27
CN110073001A (zh) 2019-07-30
WO2018065785A1 (en) 2018-04-12
EP3523438A1 (en) 2019-08-14

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]