BR112019006384A2 - enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas - Google Patents
enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmasInfo
- Publication number
- BR112019006384A2 BR112019006384A2 BR112019006384A BR112019006384A BR112019006384A2 BR 112019006384 A2 BR112019006384 A2 BR 112019006384A2 BR 112019006384 A BR112019006384 A BR 112019006384A BR 112019006384 A BR112019006384 A BR 112019006384A BR 112019006384 A2 BR112019006384 A2 BR 112019006384A2
- Authority
- BR
- Brazil
- Prior art keywords
- casx
- nucleic acids
- acids encoding
- proteins
- encoding
- Prior art date
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 5
- 101150038500 cas9 gene Proteins 0.000 abstract 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 abstract 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/06—Fusion polypeptide containing a localisation/targetting motif containing a lysosomal/endosomal localisation signal
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- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13041—Use of virus, viral particle or viral elements as a vector
- C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Abstract
a presente divulgação fornece proteínas casx, ácidos nucleicos que codificam as proteínas casx e células hospedeiras modificadas que compreendem as proteínas casx e/ou os ácidos nucleicos que as codificam. as proteínas casx são úteis em uma variedade de aplicações, que são fornecidas. a presente divulgação fornece rnas guia de casx que se ligam e fornecem especificidade de sequência às proteínas casx, ácidos nucleicos que codificam os rnas guia de casx e células hospedeiras modificadas que compreendem os rnas guia de casx e/ou ácidos nucleicos que as codificam. os rnas guia de casx são úteis em uma variedade de aplicações, que são fornecidas. a presente divulgação fornece polipeptídeos cas9 do domínio archaea e ácidos nucleicos que codificam os mesmos, bem como seus rnas guia de cas9 associados do domínio archaea e ácidos nucleicos que codificam os mesmos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662402846P | 2016-09-30 | 2016-09-30 | |
PCT/US2017/054081 WO2018064371A1 (en) | 2016-09-30 | 2017-09-28 | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112019006384A2 true BR112019006384A2 (pt) | 2019-06-25 |
Family
ID=61760962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112019006384A BR112019006384A2 (pt) | 2016-09-30 | 2017-09-28 | enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas |
Country Status (13)
Country | Link |
---|---|
US (3) | US11873504B2 (pt) |
EP (1) | EP3523426A4 (pt) |
JP (2) | JP2019532644A (pt) |
KR (2) | KR20230170126A (pt) |
CN (1) | CN110023494A (pt) |
AU (1) | AU2017335890B2 (pt) |
BR (1) | BR112019006384A2 (pt) |
CA (1) | CA3038960A1 (pt) |
EA (1) | EA201990861A1 (pt) |
GB (1) | GB2569733B (pt) |
IL (1) | IL265598A (pt) |
MX (1) | MX2019003674A (pt) |
WO (1) | WO2018064371A1 (pt) |
Families Citing this family (81)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
CA2963820A1 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
JP7030522B2 (ja) | 2015-05-11 | 2022-03-07 | エディタス・メディシン、インコーポレイテッド | 幹細胞における遺伝子編集のための最適化crispr/cas9システムおよび方法 |
WO2016201047A1 (en) | 2015-06-09 | 2016-12-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
EP3786294A1 (en) | 2015-09-24 | 2021-03-03 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
EP3365357B1 (en) | 2015-10-23 | 2024-02-14 | President and Fellows of Harvard College | Evolved cas9 proteins for gene editing |
US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
WO2018064352A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
WO2018064371A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
JP2020513783A (ja) * | 2017-01-18 | 2020-05-21 | エクシジョン バイオセラピューティクス インコーポレイテッド | Crispr |
WO2018152418A1 (en) * | 2017-02-17 | 2018-08-23 | Temple University - Of The Commonwealth System Of Higher Education | Gene editing therapy for hiv infection via dual targeting of hiv genome and ccr5 |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
EP3615672A1 (en) | 2017-04-28 | 2020-03-04 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
WO2018202800A1 (en) * | 2017-05-03 | 2018-11-08 | Kws Saat Se | Use of crispr-cas endonucleases for plant genome engineering |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
EP3635104A1 (en) | 2017-06-09 | 2020-04-15 | Editas Medicine, Inc. | Engineered cas9 nucleases |
WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
US11578334B2 (en) * | 2017-10-25 | 2023-02-14 | Monsanto Technology Llc | Targeted endonuclease activity of the RNA-guided endonuclease CasX in eukaryotes |
US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
CA3080493A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casz compositions and methods of use |
US11661599B1 (en) | 2017-12-14 | 2023-05-30 | National Technology & Engineering Solutions Of Sandia, Llc | CRISPR-Cas based system for targeting single-stranded sequences |
US11807877B1 (en) | 2018-03-22 | 2023-11-07 | National Technology & Engineering Solutions Of Sandia, Llc | CRISPR/Cas activity assays and compositions thereof |
US20210198660A1 (en) | 2018-06-07 | 2021-07-01 | Arc Bio, Llc | Compositions and methods for making guide nucleic acids |
US20190390193A1 (en) * | 2018-06-23 | 2019-12-26 | John Lawrence Mee | Reversible method for sustainable human cognitive enhancement |
US20210284981A1 (en) * | 2018-07-24 | 2021-09-16 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
EP3830301A1 (en) | 2018-08-01 | 2021-06-09 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
EP3841205A4 (en) * | 2018-08-22 | 2022-08-17 | The Regents of The University of California | VARIANT TYPE CRISPR/CAS V EFFECTIVE POLYPEPTIDES AND METHODS OF USE THEREOF |
JP2022514493A (ja) * | 2018-12-14 | 2022-02-14 | パイオニア ハイ-ブレッド インターナショナル, インコーポレイテッド | ゲノム編集のための新規なcrispr-casシステム |
WO2020142754A2 (en) | 2019-01-04 | 2020-07-09 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
MX2021008487A (es) * | 2019-01-14 | 2021-11-12 | Univ Rochester | Escisión y poliadenilación del arn nuclear dirigido con crispr-cas. |
CN113811607A (zh) | 2019-03-07 | 2021-12-17 | 加利福尼亚大学董事会 | CRISPR-Cas效应子多肽和其使用方法 |
CN113825834A (zh) | 2019-03-11 | 2021-12-21 | 索伦托药业有限公司 | 使用rna引导的核酸内切酶进行dna构建体整合的改进工艺 |
JP2022526908A (ja) | 2019-03-19 | 2022-05-27 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
US20220195455A1 (en) * | 2019-04-03 | 2022-06-23 | University Of Georgia Research Foundation, Inc. | Delivery of crispr/mcas9 through extracellular vesicles for genome editing |
KR20220032050A (ko) | 2019-06-07 | 2022-03-15 | 스크라이브 테라퓨틱스 인크. | 조작된 casx 시스템 |
AU2020311438A1 (en) * | 2019-07-11 | 2021-12-16 | Arbor Biotechnologies, Inc. | Novel CRISPR DNA targeting enzymes and systems |
JP2021016370A (ja) * | 2019-07-23 | 2021-02-15 | 株式会社東芝 | 核酸導入キャリア、核酸導入キャリアセット、核酸導入組成物及び核酸導入方法 |
KR20220070456A (ko) * | 2019-09-09 | 2022-05-31 | 스크라이브 테라퓨틱스 인크. | 면역치료법에 사용하기 위한 조성물 및 방법 |
EP4028522A1 (en) * | 2019-09-09 | 2022-07-20 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of sod1 |
CN110669795A (zh) * | 2019-10-18 | 2020-01-10 | 福州大学 | 一种在鱼类胚胎中实现精确定点rna剪切的技术 |
EP4069845A1 (en) | 2019-12-06 | 2022-10-12 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of rhodopsin |
WO2021113772A1 (en) | 2019-12-06 | 2021-06-10 | Scribe Therapeutics Inc. | Particle delivery systems |
WO2021113769A1 (en) | 2019-12-07 | 2021-06-10 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of htt |
CA3158403A1 (en) * | 2019-12-23 | 2021-07-01 | Jennifer A. Doudna | Crispr-cas effector polypeptides and methods of use thereof |
CN114901816A (zh) * | 2019-12-30 | 2022-08-12 | 帝斯曼知识产权资产管理有限公司 | 脂肪酶修饰的菌株 |
CA3163714A1 (en) | 2020-01-10 | 2021-07-15 | Benjamin OAKES | Compositions and methods for the targeting of pcsk9 |
US20230116223A1 (en) * | 2020-01-29 | 2023-04-13 | Jenthera Therapeutics Inc. | Nuclease-scaffold composition delivery platform |
JP2023515573A (ja) | 2020-02-26 | 2023-04-13 | ソレント・セラピューティクス・インコーポレイテッド | 普遍的な遮蔽性部分を有する活性化可能な抗原結合性タンパク質 |
WO2021182474A1 (ja) * | 2020-03-12 | 2021-09-16 | 株式会社Frest | オリゴヌクレオチド及び標的rnaの部位特異的編集方法 |
EP4121535A1 (en) * | 2020-03-18 | 2023-01-25 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of c9orf72 |
EP4139345A1 (en) | 2020-04-24 | 2023-03-01 | Sorrento Therapeutics, Inc. | Memory dimeric antigen receptors |
AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
WO2022026928A1 (en) * | 2020-07-30 | 2022-02-03 | Adarx Pharmaceuticals, Inc. | Adar dependent editing compositions and methods of use thereof |
US20220162625A1 (en) * | 2020-11-11 | 2022-05-26 | Monsanto Technology Llc | Methods to improve site-directed integration frequency |
AU2021392719A1 (en) * | 2020-12-03 | 2023-06-22 | Scribe Therapeutics Inc. | Engineered class 2 type v crispr systems |
US20240100185A1 (en) * | 2020-12-03 | 2024-03-28 | Scribe Therapeutics Inc. | Compositions and methods for the targeting of ptbp1 |
KR20230129395A (ko) * | 2020-12-09 | 2023-09-08 | 스크라이브 테라퓨틱스 인크. | 유전자 편집을 위한 aav 벡터 |
KR20240011120A (ko) | 2020-12-22 | 2024-01-25 | 크로마 메디슨, 인크. | 후성유전학적 편집을 위한 조성물 및 방법 |
CA3222023A1 (en) | 2021-06-01 | 2022-12-08 | Arbor Biotechnologies, Inc. | Gene editing systems comprising a crispr nuclease and uses thereof |
WO2022261149A2 (en) | 2021-06-09 | 2022-12-15 | Scribe Therapeutics Inc. | Particle delivery systems |
TW202320864A (zh) * | 2021-09-21 | 2023-06-01 | 美商斯奎柏治療公司 | 經工程改造之casx抑制子系統 |
CN113969281B (zh) * | 2021-12-24 | 2022-04-01 | 汕头大学 | 经修饰的CrRNA片段及非洲猪瘟病毒试剂盒 |
Family Cites Families (85)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
JP2004521606A (ja) | 2000-05-24 | 2004-07-22 | サード・ウェーブ・テクノロジーズ・インク | Rnaの検出法 |
US6773885B1 (en) | 2000-09-29 | 2004-08-10 | Integrated Dna Technologies, Inc. | Compositions and methods for visual ribonuclease detection assays |
CN1886512A (zh) * | 2002-04-23 | 2006-12-27 | 斯克里普斯研究所 | 多肽在叶绿体中的表达以及用于表达多肽的组合物和方法 |
EP1580273A1 (en) | 2004-03-26 | 2005-09-28 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Peptide-based method for monitoring gene expression in a host cell |
CN101283089A (zh) | 2005-10-07 | 2008-10-08 | 加利福尼亚大学董事会 | 修饰细胞色素p450酶的编码核酸和其应用方法 |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
WO2010075303A1 (en) | 2008-12-23 | 2010-07-01 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Splicing factors with a puf protein rna-binding domain and a splicing effector domain and uses of same |
WO2011100749A2 (en) | 2010-02-15 | 2011-08-18 | Cascade Biosystems, Inc. | Methods and materials for detecting viral or microbial infections |
EP2580354A4 (en) | 2010-06-11 | 2013-10-30 | Pathogenica Inc | NUCLEIC ACIDS FOR MULTIPLEX DETECTION OF ORGANISMS AND METHOD FOR THEIR USE AND MANUFACTURE |
US9193827B2 (en) | 2010-08-26 | 2015-11-24 | Massachusetts Institute Of Technology | Poly(beta-amino alcohols), their preparation, and uses thereof |
WO2012068627A1 (en) | 2010-11-24 | 2012-05-31 | The University Of Western Australia | Peptides for the specific binding of rna targets |
US9730967B2 (en) * | 2011-02-04 | 2017-08-15 | Katherine Rose Kovarik | Method and system for treating cancer cachexia |
US8815782B2 (en) | 2011-11-11 | 2014-08-26 | Agilent Technologies, Inc. | Use of DNAzymes for analysis of an RNA sample |
CA2873073A1 (en) | 2012-04-16 | 2013-10-24 | The University Of Western Australia | Peptides for the binding of nucleotide targets |
LT3401400T (lt) | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
EP3138911B1 (en) * | 2012-12-06 | 2018-12-05 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
DK2898075T3 (en) | 2012-12-12 | 2016-06-27 | Broad Inst Inc | CONSTRUCTION AND OPTIMIZATION OF IMPROVED SYSTEMS, PROCEDURES AND ENZYME COMPOSITIONS FOR SEQUENCE MANIPULATION |
ES2658401T3 (es) | 2012-12-12 | 2018-03-09 | The Broad Institute, Inc. | Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas |
WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
PT2921557T (pt) | 2012-12-12 | 2016-10-19 | Massachusetts Inst Technology | Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências |
EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
PL2784162T3 (pl) | 2012-12-12 | 2016-01-29 | Broad Inst Inc | Opracowanie systemów, metod oraz zoptymalizowanych kompozycji przewodnikowych do manipulacji sekwencyjnej |
EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
ES2576128T3 (es) | 2012-12-12 | 2016-07-05 | The Broad Institute, Inc. | Modificación por tecnología genética y optimización de sistemas, métodos y composiciones para la manipulación de secuencias con dominios funcionales |
BR102013032129B1 (pt) | 2012-12-13 | 2022-06-07 | Dow Agrosciences Llc | Método para identificar a presença de um polinucleotídeo de dna doador exógeno inserido dentro de um único locus genômico eucariótico alvo |
CA3081054A1 (en) | 2012-12-17 | 2014-06-26 | President And Fellows Of Harvard College | Rna-guided human genome engineering |
WO2014118272A1 (en) | 2013-01-30 | 2014-08-07 | Santaris Pharma A/S | Antimir-122 oligonucleotide carbohydrate conjugates |
AU2014218621B2 (en) | 2013-02-25 | 2019-11-07 | Sangamo Therapeutics, Inc. | Methods and compositions for enhancing nuclease-mediated gene disruption |
KR101780885B1 (ko) | 2013-03-14 | 2017-10-11 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
US20140349400A1 (en) | 2013-03-15 | 2014-11-27 | Massachusetts Institute Of Technology | Programmable Modification of DNA |
US20140364333A1 (en) | 2013-03-15 | 2014-12-11 | President And Fellows Of Harvard College | Methods for Live Imaging of Cells |
US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
CA2906553C (en) | 2013-03-15 | 2022-08-02 | The General Hospital Corporation | Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
US20140273230A1 (en) | 2013-03-15 | 2014-09-18 | Sigma-Aldrich Co., Llc | Crispr-based genome modification and regulation |
MX2015011985A (es) | 2013-03-15 | 2016-04-07 | Univ Minnesota | Ingenieria genomica de plantas utilizando sistemas crispr/cas. |
CN105263312A (zh) | 2013-04-05 | 2016-01-20 | 美国陶氏益农公司 | 用于在植物基因组内整合外源序列的方法和组合物 |
BR112015026197B1 (pt) | 2013-04-16 | 2022-12-06 | Regeneron Pharmaceuticals, Inc | Método para modificação marcada de um lócus genômico de interesse em uma célula de rato pluripotente |
CA2910427C (en) | 2013-05-10 | 2024-02-20 | Sangamo Biosciences, Inc. | Delivery methods and compositions for nuclease-mediated genome engineering |
EP2999788B1 (en) | 2013-05-22 | 2020-07-08 | Northwestern University | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
US20140356956A1 (en) | 2013-06-04 | 2014-12-04 | President And Fellows Of Harvard College | RNA-Guided Transcriptional Regulation |
EP3071695A2 (en) * | 2013-11-18 | 2016-09-28 | Crispr Therapeutics AG | Crispr-cas system materials and methods |
US9994831B2 (en) | 2013-12-12 | 2018-06-12 | The Regents Of The University Of California | Methods and compositions for modifying a single stranded target nucleic acid |
US9850525B2 (en) | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
EP3126498A4 (en) | 2014-03-20 | 2017-08-23 | Université Laval | Crispr-based methods and products for increasing frataxin levels and uses thereof |
EP3129488B1 (en) | 2014-04-10 | 2019-06-12 | The Regents of The University of California | Methods and compositions for using argonaute to modify a single stranded target nucleic acid |
CA2951707A1 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
KR20170036801A (ko) | 2014-08-19 | 2017-04-03 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산의 프로빙 및 맵핑을 위한 rna-가이드된 시스템 |
EP3985115A1 (en) | 2014-12-12 | 2022-04-20 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
WO2016094872A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Dead guides for crispr transcription factors |
WO2016106236A1 (en) | 2014-12-23 | 2016-06-30 | The Broad Institute Inc. | Rna-targeting system |
US11180792B2 (en) * | 2015-01-28 | 2021-11-23 | The Regents Of The University Of California | Methods and compositions for labeling a single-stranded target nucleic acid |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
AU2016280893B2 (en) | 2015-06-18 | 2021-12-02 | Massachusetts Institute Of Technology | CRISPR enzyme mutations reducing off-target effects |
WO2016205749A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
CA3012631A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Novel crispr enzymes and systems |
US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
US20170211142A1 (en) | 2015-10-22 | 2017-07-27 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
EP3400299B1 (en) | 2016-01-08 | 2020-11-11 | University of Georgia Research Foundation, Inc. | Methods for cleaving dna and rna molecules |
US11441146B2 (en) * | 2016-01-11 | 2022-09-13 | Christiana Care Health Services, Inc. | Compositions and methods for improving homogeneity of DNA generated using a CRISPR/Cas9 cleavage system |
US9896696B2 (en) | 2016-02-15 | 2018-02-20 | Benson Hill Biosystems, Inc. | Compositions and methods for modifying genomes |
US20190024075A1 (en) | 2016-02-23 | 2019-01-24 | Arc Bio, Llc | Methods and compositions for target detection |
US20190093091A1 (en) | 2016-04-06 | 2019-03-28 | Temple University - Of The Commonwealth System Of Higher Education | Compositions for eradicating flavivirus infections in subjects |
WO2017205668A1 (en) | 2016-05-25 | 2017-11-30 | Arizona Board Of Regents On Behalf Of Arizona State University | Portable, low-cost pathogen detection and strain identification platform |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
JP7267013B2 (ja) | 2016-06-17 | 2023-05-01 | ザ・ブロード・インスティテュート・インコーポレイテッド | Vi型crisprオルソログ及び系 |
AU2017280353B2 (en) | 2016-06-24 | 2021-11-11 | Inscripta, Inc. | Methods for generating barcoded combinatorial libraries |
US20210166783A1 (en) | 2016-08-17 | 2021-06-03 | The Broad Institute, Inc. | Methods for identifying class 2 crispr-cas systems |
WO2018064352A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
WO2018064371A1 (en) | 2016-09-30 | 2018-04-05 | The Regents Of The University Of California | Rna-guided nucleic acid modifying enzymes and methods of use thereof |
CN106701830B (zh) | 2016-12-07 | 2020-01-03 | 湖南人文科技学院 | 一种敲除猪胚胎p66shc基因的方法 |
EP4119663A1 (en) | 2016-12-09 | 2023-01-18 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
AU2018240515A1 (en) | 2017-03-24 | 2019-08-01 | CureVac SE | Nucleic acids encoding CRISPR-associated proteins and uses thereof |
WO2018195545A2 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Variants of cpf1 (cas12a) with altered pam specificity |
ES2960390T3 (es) | 2017-08-09 | 2024-03-04 | Ricetec Inc | Composiciones y métodos para modificar genomas |
US20200255858A1 (en) | 2017-11-01 | 2020-08-13 | Jillian F. Banfield | Casy compositions and methods of use |
CA3080493A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Casz compositions and methods of use |
GB2582100B (en) | 2017-11-01 | 2023-05-17 | Univ California | CAS12C Compositions and methods of use |
US11970719B2 (en) | 2017-11-01 | 2024-04-30 | The Regents Of The University Of California | Class 2 CRISPR/Cas compositions and methods of use |
US10253365B1 (en) | 2017-11-22 | 2019-04-09 | The Regents Of The University Of California | Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs |
CN111836903A (zh) | 2017-12-22 | 2020-10-27 | 博德研究所 | 基于crispr效应***的多重诊断 |
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GB201905575D0 (en) | 2019-06-05 |
KR20230170126A (ko) | 2023-12-18 |
US11795472B2 (en) | 2023-10-24 |
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