BR112018009729A2 - método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico - Google Patents

método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico

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Publication number
BR112018009729A2
BR112018009729A2 BR112018009729A BR112018009729A BR112018009729A2 BR 112018009729 A2 BR112018009729 A2 BR 112018009729A2 BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A2 BR112018009729 A2 BR 112018009729A2
Authority
BR
Brazil
Prior art keywords
recombinant polypeptide
filamentous fungus
producing
nucleic acid
myceliophthora thermophila
Prior art date
Application number
BR112018009729A
Other languages
English (en)
Other versions
BR112018009729A8 (pt
Inventor
Thywissen Andreas
hartmann Holger
Boehmer Nico
Haefner Stefan
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of BR112018009729A2 publication Critical patent/BR112018009729A2/pt
Publication of BR112018009729A8 publication Critical patent/BR112018009729A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2491—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21063—Oryzin (3.4.21.63)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

a presente invenção diz respeito a um método de produção de um polipeptídeo recombinante em um fungo filamentoso que é geneticamente modificado para diminuir ou eliminar a atividade do regulador da celulase 2 (clr2) e para expressar o referido polipeptídeo recombinante. o método diz respeito ainda a um fungo filamentoso myceliophthora thermophila que é geneticamente modificado para diminuir ou eliminar a atividade do clr2 e ao uso deste fungo filamentoso na produção de um polipeptídeo recombinante.
BR112018009729A 2015-12-02 2016-12-02 método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico BR112018009729A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15197497 2015-12-02
PCT/EP2016/079526 WO2017093450A1 (en) 2015-12-02 2016-12-02 Method of producing proteins in filamentous fungi with decreased clr2 activity

Publications (2)

Publication Number Publication Date
BR112018009729A2 true BR112018009729A2 (pt) 2018-11-21
BR112018009729A8 BR112018009729A8 (pt) 2019-02-26

Family

ID=54838169

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018009729A BR112018009729A8 (pt) 2015-12-02 2016-12-02 método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico

Country Status (9)

Country Link
US (1) US11299522B2 (pt)
EP (1) EP3384002A1 (pt)
JP (1) JP2019503168A (pt)
KR (1) KR20180084135A (pt)
CN (1) CN108291189A (pt)
BR (1) BR112018009729A8 (pt)
CA (1) CA3004990A1 (pt)
MX (1) MX2018006535A (pt)
WO (1) WO2017093450A1 (pt)

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MX2018006535A (es) 2015-12-02 2018-08-15 Basf Se Metodo para producir proteinas en hongos filamentosos con accion clr2 reducida.
CN108291191A (zh) 2015-12-02 2018-07-17 巴斯夫欧洲公司 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法
WO2019038623A1 (en) 2017-08-21 2019-02-28 Dyadic International Inc. PRODUCTION OF AN INFLUENZA VACCINE IN MYCELIOPHTHORA THERMOPHILA
CN110042116A (zh) * 2019-04-30 2019-07-23 贵州大学 一种稻瘟菌生物钟控制基因MoCCG4的重组载体和表达方法
CN112813091A (zh) * 2021-02-03 2021-05-18 山东农业大学 Nat1基因作为筛选标记在卵菌遗传转化中的应用
CN113151264A (zh) * 2021-03-11 2021-07-23 上海中溶科技有限公司 一种利用正向调控基因构建高产纤维素酶菌株的方法及应用

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WO2015135980A1 (en) 2014-03-11 2015-09-17 Basf Se The use of polymers to improve the rheology of base suspensions during fermentation
KR20160134760A (ko) 2014-03-19 2016-11-23 바스프 에스이 발효를 위한 탄수화물의 제한적 공급을 사용하는 글리세롤의 용도
EP3140412A1 (en) 2014-05-08 2017-03-15 Basf Se Genetically modified microorganism for improved production of fine chemicals on sucrose
WO2015177674A1 (en) 2014-05-21 2015-11-26 Basf Se Gene cluster for biosynthesis of cornexistin and hydroxycornexistin
WO2016030373A1 (en) 2014-08-28 2016-03-03 Basf Se Modified microorganism for improved production of fine chemicals on sucrose
EP3230435B1 (en) 2014-12-10 2023-11-29 Basf Se Method of removing dna from biotechnological products
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MX2018006535A (es) 2015-12-02 2018-08-15 Basf Se Metodo para producir proteinas en hongos filamentosos con accion clr2 reducida.
CN108291191A (zh) 2015-12-02 2018-07-17 巴斯夫欧洲公司 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法

Also Published As

Publication number Publication date
CA3004990A1 (en) 2017-06-08
BR112018009729A8 (pt) 2019-02-26
JP2019503168A (ja) 2019-02-07
KR20180084135A (ko) 2018-07-24
MX2018006535A (es) 2018-08-15
US11299522B2 (en) 2022-04-12
EP3384002A1 (en) 2018-10-10
US20200308235A1 (en) 2020-10-01
WO2017093450A1 (en) 2017-06-08
CN108291189A (zh) 2018-07-17

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2699 DE 27-09-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.