BR112017024855A2 - métodos de produção de aldc - Google Patents

métodos de produção de aldc

Info

Publication number
BR112017024855A2
BR112017024855A2 BR112017024855A BR112017024855A BR112017024855A2 BR 112017024855 A2 BR112017024855 A2 BR 112017024855A2 BR 112017024855 A BR112017024855 A BR 112017024855A BR 112017024855 A BR112017024855 A BR 112017024855A BR 112017024855 A2 BR112017024855 A2 BR 112017024855A2
Authority
BR
Brazil
Prior art keywords
aldc
production methods
improved
kits
microorganisms
Prior art date
Application number
BR112017024855A
Other languages
English (en)
Inventor
Hemmingsen Kellett-Smith Anja
Larsen Bjarne
Flyvholm Cramer Jacob
Bojsen Jensen Lene
Original Assignee
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Nutrition Biosci Aps filed Critical Dupont Nutrition Biosci Aps
Publication of BR112017024855A2 publication Critical patent/BR112017024855A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

a presente divulgação fornece métodos, composições, aparelhos e kits que compreendem enzimas aldc que têm uma melhor estabilidade e atividade, e que, ainda, podem ser recuperadas de micro-organismos em rendimentos aprimorados.
BR112017024855A 2015-05-22 2016-05-18 métodos de produção de aldc BR112017024855A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562165690P 2015-05-22 2015-05-22
US201562166616P 2015-05-26 2015-05-26
US201562168415P 2015-05-29 2015-05-29
PCT/US2016/033043 WO2016191170A1 (en) 2015-05-22 2016-05-18 Aldc production methods

Publications (1)

Publication Number Publication Date
BR112017024855A2 true BR112017024855A2 (pt) 2018-08-07

Family

ID=56121168

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017024855A BR112017024855A2 (pt) 2015-05-22 2016-05-18 métodos de produção de aldc

Country Status (8)

Country Link
US (2) US20180142228A1 (pt)
EP (2) EP3298137A1 (pt)
JP (1) JP7320920B2 (pt)
CN (1) CN107667172A (pt)
BR (1) BR112017024855A2 (pt)
CA (1) CA2986695A1 (pt)
MX (1) MX2017014890A (pt)
WO (1) WO2016191170A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106282079B (zh) * 2016-08-10 2019-12-31 中国农业科学院饲料研究所 一套高效分泌表达异源蛋白的工程菌及其应用
DK3512941T3 (da) 2016-09-16 2021-06-28 Dupont Nutrition Biosci Aps Acetolactatdecarboxylasevarianter med forbedret specifik aktivitet
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus
CN117660420A (zh) * 2023-10-20 2024-03-08 山东省农业科学院 一种微小杆菌凝乳酶及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK149335C (da) 1983-06-03 1986-10-20 Novo Industri As Alpha-acetolactat-decarboxylase samt fremgangsmaade til fremstilling deraf
DK0551386T3 (da) 1990-10-05 2004-05-10 Genencor Int Fremgangsmåde til behandling af bomuldsholdige stoffer med cellulase
US5246853A (en) 1990-10-05 1993-09-21 Genencor International, Inc. Method for treating cotton-containing fabric with a cellulase composition containing endoglucanase components and which composition is free of exo-cellobiohydrolase I
US5475101A (en) 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US20020182734A1 (en) 2000-08-11 2002-12-05 Diaz-Torres Maria R. Bacillus transformation, transformants and mutant libraries
JP4485341B2 (ja) * 2004-12-20 2010-06-23 花王株式会社 組換え微生物
JP2009038985A (ja) * 2007-08-06 2009-02-26 Kao Corp 微生物及びこれを用いたタンパク質又ポリペプチドの製造方法
GB0715936D0 (en) * 2007-08-15 2007-09-26 Cobra Biolog Ltd Mutant bacterium
CN101874110A (zh) * 2007-10-31 2010-10-27 丹尼斯科美国公司 中性金属蛋白酶在无丝氨酸蛋白酶背景中的用途和生产
CA2705334C (en) * 2007-11-12 2018-04-17 Novozymes A/S Dual affinity polypeptides for purification
JP6059416B2 (ja) * 2010-02-03 2017-01-11 花王株式会社 溶菌が抑制された微生物、当該微生物の製造方法、当該微生物を用いたタンパク質等の製造方法、及び微生物の溶菌抑制方法
CN101948866A (zh) * 2010-09-03 2011-01-19 南宁邦尔克生物技术有限责任公司 一种能用同源重组来克隆的枯草芽孢杆菌整合载体的构建及其应用
CN101948821B (zh) 2010-09-03 2012-09-12 南宁邦尔克生物技术有限责任公司 一种以整合型重组枯草芽孢杆菌为菌种生产α-乙酰乳酸脱羧酶的方法
JP2012115219A (ja) * 2010-12-02 2012-06-21 Kao Corp エンドグルカナーゼの製造法
WO2012127002A1 (en) 2011-03-23 2012-09-27 Novozymes A/S Sweet-tasting polypeptide from gram-positive bacteria
JP5753419B2 (ja) * 2011-03-24 2015-07-22 花王株式会社 遺伝子欠損株及びそれを用いたタンパク質の製造方法
CN102876704A (zh) * 2012-09-20 2013-01-16 江南大学 利用重组枯草芽孢杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
US20160122772A1 (en) * 2013-06-21 2016-05-05 Novozymes A/S Production of Polypeptides Without Secretion Signal in Bacillus

Also Published As

Publication number Publication date
CN107667172A (zh) 2018-02-06
JP7320920B2 (ja) 2023-08-04
JP2018516076A (ja) 2018-06-21
CA2986695A1 (en) 2016-12-01
EP3805384A1 (en) 2021-04-14
WO2016191170A1 (en) 2016-12-01
US20180142228A1 (en) 2018-05-24
US20230295601A1 (en) 2023-09-21
EP3298137A1 (en) 2018-03-28
MX2017014890A (es) 2018-04-20

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]