BR112017018885A2 - método de cultura celular para células-tronco mesenquimais - Google Patents

método de cultura celular para células-tronco mesenquimais

Info

Publication number
BR112017018885A2
BR112017018885A2 BR112017018885A BR112017018885A BR112017018885A2 BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2 BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A BR112017018885 A BR 112017018885A BR 112017018885 A2 BR112017018885 A2 BR 112017018885A2
Authority
BR
Brazil
Prior art keywords
cell culture
stem cells
mesenchymal stem
culture method
compositions
Prior art date
Application number
BR112017018885A
Other languages
English (en)
Inventor
Suire Colby
Simmons Paul
Original Assignee
Mesoblast Int Sarl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015900752A external-priority patent/AU2015900752A0/en
Application filed by Mesoblast Int Sarl filed Critical Mesoblast Int Sarl
Publication of BR112017018885A2 publication Critical patent/BR112017018885A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/115—Platelets, megakaryocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

a presente divulgação refere-se a métodos, meios de cultura de células e composições que promovem a proliferação celular durante a cultura de células sem soro bovino fetal.
BR112017018885A 2015-03-04 2016-03-04 método de cultura celular para células-tronco mesenquimais BR112017018885A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015900752A AU2015900752A0 (en) 2015-03-04 Cell culture method
AU2015900777A AU2015900777A0 (en) 2015-03-05 Cell culture method
PCT/EP2016/054640 WO2016139340A1 (en) 2015-03-04 2016-03-04 Cell culture method for mesenchymal stem cells

Publications (1)

Publication Number Publication Date
BR112017018885A2 true BR112017018885A2 (pt) 2018-04-17

Family

ID=55453191

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017018885A BR112017018885A2 (pt) 2015-03-04 2016-03-04 método de cultura celular para células-tronco mesenquimais

Country Status (10)

Country Link
US (2) US20180037867A1 (pt)
EP (2) EP4008775A1 (pt)
JP (3) JP6885870B2 (pt)
KR (1) KR102635212B1 (pt)
CN (2) CN113957043A (pt)
AU (2) AU2016227607B2 (pt)
BR (1) BR112017018885A2 (pt)
CA (1) CA2977578A1 (pt)
SG (1) SG11201706886PA (pt)
WO (1) WO2016139340A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2334785B2 (en) 2008-09-16 2019-08-14 Mayo Foundation For Medical Education And Research Compositions containing platelet contents
EP4328299A3 (en) 2014-05-16 2024-05-29 Mayo Foundation for Medical Education and Research Cell culture media compositions for primary cells
CN108251358B (zh) * 2017-12-15 2021-10-26 广东药科大学 一种相同供体来源人间充质干细胞的多批次原代分离方法
US20210115395A1 (en) * 2018-03-28 2021-04-22 Pluristem Ltd. Methods for expanding adherent stromal cells and cells obtained thereby
CN108624554B (zh) * 2018-05-14 2021-09-14 山东大学 一种非基因修饰光操控双向调节人脐带间充质干细胞增殖的方法
US11364323B2 (en) 2018-09-17 2022-06-21 Rejuvablast LLC Combination grafts for tissue repair or regeneration applications
WO2020067443A1 (ja) * 2018-09-27 2020-04-02 テルモ株式会社 体細胞のシート化方法
CN115997005A (zh) * 2020-06-11 2023-04-21 迈索布拉斯特国际有限公司 间充质谱系前体或干细胞的3d培养
WO2022192434A1 (en) * 2021-03-10 2022-09-15 Terasaki Institute For Biomedical Innovation Methods and systems of preparing cultivated meat from blood or cellular biomass

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US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
WO1993003679A1 (de) 1991-08-12 1993-03-04 Karl Storz Gmbh & Co. Hochfrequenzchirurgiegenerator zum geregelten schneiden und koagulieren
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
ATE342348T1 (de) * 1998-11-09 2006-11-15 Consorzio Per La Gestione Del Serum-freies medium für chondrozyt-ähnliche zellen
AU2003901668A0 (en) 2003-03-28 2003-05-01 Medvet Science Pty. Ltd. Non-haemopoietic precursor cells
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
WO2007027156A1 (en) * 2005-09-02 2007-03-08 Agency For Science, Technology And Research Method of deriving mesenchymal stem cells
KR101107835B1 (ko) * 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
WO2007099337A1 (en) * 2006-03-01 2007-09-07 Cartela R&D Ab Expansion and differentiation of mesenchymal stem cells
JP2010500047A (ja) * 2006-08-15 2010-01-07 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 間葉系幹細胞馴化培地
JP2008148643A (ja) * 2006-12-19 2008-07-03 Olympus Corp 無血清培地および幹細胞の培養方法
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
KR101224588B1 (ko) * 2008-01-24 2013-01-22 삼성전자주식회사 멀티포인트 스트록을 감지하기 위한 ｕｉ 제공방법 및 이를적용한 멀티미디어 기기
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
CN102791276B (zh) * 2010-03-10 2015-03-04 智再如股份有限公司 含有间充质干细胞的细胞制品及其制造方法
CN103068969A (zh) * 2010-06-17 2013-04-24 思迪公司 化学成分确定的无血清细胞培养基
AU2012206411B2 (en) * 2011-01-12 2017-05-18 Tsuneo KIDO Culture method to obtain and maintain a pure or enriched population of mammalian neural stem cells and/or neural progenitor cells that are prone to differentiate into oligodendrocyte-lineage cells
KR101523040B1 (ko) * 2012-06-26 2015-05-26 라정찬 고농도의 줄기세포 제조방법
WO2014082685A1 (en) * 2012-11-30 2014-06-05 Swiss Stem Cell Foundation Serum-free medium for human mesenchymal stem cells
CN103396984B (zh) * 2013-07-25 2016-02-10 宗兆文 一种利用皮肤来源的祖细胞制备组织工程脊髓的方法

Also Published As

Publication number Publication date
AU2021254538A1 (en) 2021-11-18
AU2016227607B2 (en) 2021-09-30
CN113957043A (zh) 2022-01-21
WO2016139340A1 (en) 2016-09-09
CA2977578A1 (en) 2016-09-09
EP3265554A1 (en) 2018-01-10
EP4008775A1 (en) 2022-06-08
KR102635212B1 (ko) 2024-02-13
US20200040303A1 (en) 2020-02-06
KR20170120627A (ko) 2017-10-31
CN107567494A (zh) 2018-01-09
AU2021254538B2 (en) 2024-01-18
US11685899B2 (en) 2023-06-27
JP2018506986A (ja) 2018-03-15
JP6885870B2 (ja) 2021-06-16
SG11201706886PA (en) 2017-09-28
AU2016227607A1 (en) 2017-09-07
US20180037867A1 (en) 2018-02-08
EP3265554B1 (en) 2021-11-17
JP2021090463A (ja) 2021-06-17
JP2024001179A (ja) 2024-01-09
JP7410899B2 (ja) 2024-01-10

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B154 Notification of filing of divisional application [chapter 15.50 patent gazette]

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