BR112017014583A2 - método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína - Google Patents

método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína

Info

Publication number
BR112017014583A2
BR112017014583A2 BR112017014583-9A BR112017014583A BR112017014583A2 BR 112017014583 A2 BR112017014583 A2 BR 112017014583A2 BR 112017014583 A BR112017014583 A BR 112017014583A BR 112017014583 A2 BR112017014583 A2 BR 112017014583A2
Authority
BR
Brazil
Prior art keywords
biomass
components
soluble fraction
microalgae biomass
protein rich
Prior art date
Application number
BR112017014583-9A
Other languages
English (en)
Other versions
BR112017014583A8 (pt
Inventor
Patinier Samuel
Original Assignee
Roquette Freres
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roquette Freres filed Critical Roquette Freres
Publication of BR112017014583A2 publication Critical patent/BR112017014583A2/pt
Publication of BR112017014583A8 publication Critical patent/BR112017014583A8/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/009—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from unicellular algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20—Synthetic spices, flavouring agents or condiments
    • A23L27/23—Synthetic spices, flavouring agents or condiments containing nucleotides
    • A23L27/235—Synthetic spices, flavouring agents or condiments containing nucleotides containing also amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/88—Taste or flavour enhancing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a invenção refere-se a um método para o fracionamento dos componentes de uma biomassa de microalgas ricas em proteína do gênero chlorella distinguido pelo fato de que compreende as seguintes etapas: - fornecer uma biomassa de microalgas produzida por fermentação, - opcionalmente, lavar a biomassa de modo a eliminar os compostos solúveis intersticiais, - permeabilizar termicamente a biomassa a uma temperatura entre 50 e 150 °c, de preferência, entre 100 e 150 °c, por um período entre 10 segundos e 5 minutos, de preferência, por um período entre 5 segundos e 1 minuto, - fazer separação entre a biomassa assim permeabilizada e a fração solúvel por meio de uma técnica de centrifugação, mais particularmente, centrifugação de múltiplos estágios, - opcionalmente, recuperar e clarificar a fração solúvel obtida dessa forma por meio de microfiltração de modo a remover substâncias insolúveis residuais da mesma, - separar a fração solúvel anterior por meio de precipitação, de modo a obter um isolado de peptídeo e um concentrado de peptídeo.
BR112017014583A 2015-01-26 2016-01-25 Método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína BR112017014583A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR1550571 2015-01-26
FR1550571A FR3031987B1 (fr) 2015-01-26 2015-01-26 Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines
PCT/FR2016/050138 WO2016120548A1 (fr) 2015-01-26 2016-01-25 Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines

Publications (2)

Publication Number Publication Date
BR112017014583A2 true BR112017014583A2 (pt) 2018-01-16
BR112017014583A8 BR112017014583A8 (pt) 2018-07-31

Family

ID=52808004

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017014583A BR112017014583A8 (pt) 2015-01-26 2016-01-25 Método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína

Country Status (9)

Country Link
US (1) US20180007932A1 (pt)
EP (1) EP3250704A1 (pt)
JP (1) JP2018502592A (pt)
KR (1) KR20170105497A (pt)
CN (1) CN107208124A (pt)
BR (1) BR112017014583A8 (pt)
FR (1) FR3031987B1 (pt)
MX (1) MX2017008936A (pt)
WO (1) WO2016120548A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170023065A (ko) 2014-07-18 2017-03-02 로께뜨프레르 미세조류 바이오매스로부터의 가용성 단백질의 추출 방법
FR3085386B1 (fr) * 2018-09-05 2022-10-07 Fermentalg Procede d'enrichissement d'une biomasse en proteines
FR3101522A1 (fr) * 2019-10-04 2021-04-09 La Belle Ondulée Procédé de transformation d’une biomasse concentrée en vue d’obtenir une préparation alimentaire.

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1138073A1 (ru) * 1976-12-02 1985-02-07 Всесоюзный Научно-Исследовательский Биотехнический Институт Способ разрушени клеточной оболочки хлореллы
JPH03240484A (ja) * 1990-02-16 1991-10-25 Baiotsukusu:Kk クロレラの培養方法
JP3143636B2 (ja) 1991-09-11 2001-03-07 株式会社サン・クロレラ 細胞破裂によるクロレラ細胞壁の破砕方法
JP2000106826A (ja) * 1998-10-01 2000-04-18 Senmi Extract Kk クロレラペプチド含有組成物
KR100657637B1 (ko) * 2005-03-08 2006-12-19 진현진 클로렐라로부터 액상 추출물을 추출하는 방법
US20100023376A1 (en) * 2008-07-28 2010-01-28 Brown Stephen J Iterative real-time auction for resource management with user rules
CN101352249B (zh) * 2008-09-04 2011-01-19 蔡志武 一种全面利用含油微藻的生产方法
JP5731982B2 (ja) * 2008-10-14 2015-06-10 ソラザイム, インコーポレイテッドSolazyme Inc 微細藻類バイオマスの食物組成物
EP2406370A4 (en) * 2009-03-10 2013-08-14 Srs Energy FRACTIONATION OF ALGAE BIOMASS
CN101736045B (zh) * 2009-12-03 2011-12-07 渤海大学 一种小球藻功能成分连续提取的方法
US10098371B2 (en) * 2013-01-28 2018-10-16 Solazyme Roquette Nutritionals, LLC Microalgal flour
ES2708839T3 (es) * 2013-03-29 2019-04-11 Corbion Biotech Inc Procedimiento de enriquecimiento en proteínas de la biomasa de microalgas
FR3003873B1 (fr) * 2013-03-29 2016-10-21 Roquette Freres Procede d'enrichissement en proteines de la biomasse de microalgues
FR3007625B1 (fr) * 2013-06-26 2015-07-17 Roquette Freres Procede de production de biomasse de microalgues de qualite sensorielle optimisee
FR3008712B1 (fr) * 2013-07-19 2016-09-16 Roquette Freres Procede optimise de rupture des parois de chlorelles par homogeneisation a tres haute pression
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KR20170023065A (ko) * 2014-07-18 2017-03-02 로께뜨프레르 미세조류 바이오매스로부터의 가용성 단백질의 추출 방법
FR3031985B1 (fr) * 2015-01-26 2017-02-17 Roquette Freres Procede d'obtention d'un isolat peptidique issu de la biomasse de microalgues enrichies en proteines

Also Published As

Publication number Publication date
MX2017008936A (es) 2018-04-11
KR20170105497A (ko) 2017-09-19
WO2016120548A1 (fr) 2016-08-04
FR3031987A1 (fr) 2016-07-29
US20180007932A1 (en) 2018-01-11
EP3250704A1 (fr) 2017-12-06
BR112017014583A8 (pt) 2018-07-31
JP2018502592A (ja) 2018-02-01
CN107208124A (zh) 2017-09-26
FR3031987B1 (fr) 2019-05-24

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Legal Events

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B25A Requested transfer of rights approved

Owner name: CORBION BIOTECH, INC. (US)

B25A Requested transfer of rights approved

Owner name: CORBION BIOTECH, INC. (US)

B25L Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: publication cancelled

Owner name: CORBION BIOTECH, INC. (US)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements