BR112017014583A2 - método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína - Google Patents
método para fracionamento de componentes de uma biomassa de microalgas ricas em proteínaInfo
- Publication number
- BR112017014583A2 BR112017014583A2 BR112017014583-9A BR112017014583A BR112017014583A2 BR 112017014583 A2 BR112017014583 A2 BR 112017014583A2 BR 112017014583 A BR112017014583 A BR 112017014583A BR 112017014583 A2 BR112017014583 A2 BR 112017014583A2
- Authority
- BR
- Brazil
- Prior art keywords
- biomass
- components
- soluble fraction
- microalgae biomass
- protein rich
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/009—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from unicellular algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/23—Synthetic spices, flavouring agents or condiments containing nucleotides
- A23L27/235—Synthetic spices, flavouring agents or condiments containing nucleotides containing also amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/88—Taste or flavour enhancing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
a invenção refere-se a um método para o fracionamento dos componentes de uma biomassa de microalgas ricas em proteína do gênero chlorella distinguido pelo fato de que compreende as seguintes etapas: - fornecer uma biomassa de microalgas produzida por fermentação, - opcionalmente, lavar a biomassa de modo a eliminar os compostos solúveis intersticiais, - permeabilizar termicamente a biomassa a uma temperatura entre 50 e 150 °c, de preferência, entre 100 e 150 °c, por um período entre 10 segundos e 5 minutos, de preferência, por um período entre 5 segundos e 1 minuto, - fazer separação entre a biomassa assim permeabilizada e a fração solúvel por meio de uma técnica de centrifugação, mais particularmente, centrifugação de múltiplos estágios, - opcionalmente, recuperar e clarificar a fração solúvel obtida dessa forma por meio de microfiltração de modo a remover substâncias insolúveis residuais da mesma, - separar a fração solúvel anterior por meio de precipitação, de modo a obter um isolado de peptídeo e um concentrado de peptídeo.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1550571 | 2015-01-26 | ||
FR1550571A FR3031987B1 (fr) | 2015-01-26 | 2015-01-26 | Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines |
PCT/FR2016/050138 WO2016120548A1 (fr) | 2015-01-26 | 2016-01-25 | Procede de fractionnement des composants d'une biomasse de microalgues riches en proteines |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112017014583A2 true BR112017014583A2 (pt) | 2018-01-16 |
BR112017014583A8 BR112017014583A8 (pt) | 2018-07-31 |
Family
ID=52808004
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017014583A BR112017014583A8 (pt) | 2015-01-26 | 2016-01-25 | Método para fracionamento de componentes de uma biomassa de microalgas ricas em proteína |
Country Status (9)
Country | Link |
---|---|
US (1) | US20180007932A1 (pt) |
EP (1) | EP3250704A1 (pt) |
JP (1) | JP2018502592A (pt) |
KR (1) | KR20170105497A (pt) |
CN (1) | CN107208124A (pt) |
BR (1) | BR112017014583A8 (pt) |
FR (1) | FR3031987B1 (pt) |
MX (1) | MX2017008936A (pt) |
WO (1) | WO2016120548A1 (pt) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20170023065A (ko) | 2014-07-18 | 2017-03-02 | 로께뜨프레르 | 미세조류 바이오매스로부터의 가용성 단백질의 추출 방법 |
FR3085386B1 (fr) * | 2018-09-05 | 2022-10-07 | Fermentalg | Procede d'enrichissement d'une biomasse en proteines |
FR3101522A1 (fr) * | 2019-10-04 | 2021-04-09 | La Belle Ondulée | Procédé de transformation d’une biomasse concentrée en vue d’obtenir une préparation alimentaire. |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1138073A1 (ru) * | 1976-12-02 | 1985-02-07 | Всесоюзный Научно-Исследовательский Биотехнический Институт | Способ разрушени клеточной оболочки хлореллы |
JPH03240484A (ja) * | 1990-02-16 | 1991-10-25 | Baiotsukusu:Kk | クロレラの培養方法 |
JP3143636B2 (ja) | 1991-09-11 | 2001-03-07 | 株式会社サン・クロレラ | 細胞破裂によるクロレラ細胞壁の破砕方法 |
JP2000106826A (ja) * | 1998-10-01 | 2000-04-18 | Senmi Extract Kk | クロレラペプチド含有組成物 |
KR100657637B1 (ko) * | 2005-03-08 | 2006-12-19 | 진현진 | 클로렐라로부터 액상 추출물을 추출하는 방법 |
US20100023376A1 (en) * | 2008-07-28 | 2010-01-28 | Brown Stephen J | Iterative real-time auction for resource management with user rules |
CN101352249B (zh) * | 2008-09-04 | 2011-01-19 | 蔡志武 | 一种全面利用含油微藻的生产方法 |
JP5731982B2 (ja) * | 2008-10-14 | 2015-06-10 | ソラザイム, インコーポレイテッドSolazyme Inc | 微細藻類バイオマスの食物組成物 |
EP2406370A4 (en) * | 2009-03-10 | 2013-08-14 | Srs Energy | FRACTIONATION OF ALGAE BIOMASS |
CN101736045B (zh) * | 2009-12-03 | 2011-12-07 | 渤海大学 | 一种小球藻功能成分连续提取的方法 |
US10098371B2 (en) * | 2013-01-28 | 2018-10-16 | Solazyme Roquette Nutritionals, LLC | Microalgal flour |
ES2708839T3 (es) * | 2013-03-29 | 2019-04-11 | Corbion Biotech Inc | Procedimiento de enriquecimiento en proteínas de la biomasa de microalgas |
FR3003873B1 (fr) * | 2013-03-29 | 2016-10-21 | Roquette Freres | Procede d'enrichissement en proteines de la biomasse de microalgues |
FR3007625B1 (fr) * | 2013-06-26 | 2015-07-17 | Roquette Freres | Procede de production de biomasse de microalgues de qualite sensorielle optimisee |
FR3008712B1 (fr) * | 2013-07-19 | 2016-09-16 | Roquette Freres | Procede optimise de rupture des parois de chlorelles par homogeneisation a tres haute pression |
FR3008581B1 (fr) * | 2013-07-19 | 2016-11-04 | Roquette Freres | Farine de microalgues riches en lipides et procede de preparation |
ES2642830T3 (es) * | 2013-07-25 | 2017-11-20 | Roquette Frères | Procedimiento de optimización del rendimiento de producción, de la calidad organoléptica y de la estabilidad en el tiempo de una biomasa de microalgas ricas en proteínas |
CN106103693A (zh) * | 2014-03-18 | 2016-11-09 | 罗盖特兄弟公司 | 用于微藻生物质的热透化的方法 |
EP3169695B1 (fr) * | 2014-07-18 | 2019-11-13 | Corbion Biotech, Inc. | Procede d'extraction des proteines solubles de biomasses de microalgues |
KR20170023065A (ko) * | 2014-07-18 | 2017-03-02 | 로께뜨프레르 | 미세조류 바이오매스로부터의 가용성 단백질의 추출 방법 |
FR3031985B1 (fr) * | 2015-01-26 | 2017-02-17 | Roquette Freres | Procede d'obtention d'un isolat peptidique issu de la biomasse de microalgues enrichies en proteines |
-
2015
- 2015-01-26 FR FR1550571A patent/FR3031987B1/fr active Active
-
2016
- 2016-01-25 EP EP16705227.3A patent/EP3250704A1/fr not_active Withdrawn
- 2016-01-25 JP JP2017539262A patent/JP2018502592A/ja active Pending
- 2016-01-25 BR BR112017014583A patent/BR112017014583A8/pt not_active Application Discontinuation
- 2016-01-25 MX MX2017008936A patent/MX2017008936A/es unknown
- 2016-01-25 CN CN201680007322.6A patent/CN107208124A/zh active Pending
- 2016-01-25 WO PCT/FR2016/050138 patent/WO2016120548A1/fr active Application Filing
- 2016-01-25 KR KR1020177017771A patent/KR20170105497A/ko unknown
- 2016-01-25 US US15/546,236 patent/US20180007932A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
MX2017008936A (es) | 2018-04-11 |
KR20170105497A (ko) | 2017-09-19 |
WO2016120548A1 (fr) | 2016-08-04 |
FR3031987A1 (fr) | 2016-07-29 |
US20180007932A1 (en) | 2018-01-11 |
EP3250704A1 (fr) | 2017-12-06 |
BR112017014583A8 (pt) | 2018-07-31 |
JP2018502592A (ja) | 2018-02-01 |
CN107208124A (zh) | 2017-09-26 |
FR3031987B1 (fr) | 2019-05-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B25A | Requested transfer of rights approved |
Owner name: CORBION BIOTECH, INC. (US) |
|
B25A | Requested transfer of rights approved |
Owner name: CORBION BIOTECH, INC. (US) |
|
B25L | Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: publication cancelled |
Owner name: CORBION BIOTECH, INC. (US) |
|
B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |