BR112016020517A2 - método de produção de lipídeo através da utilização de ss-cetoacil-acp sintase - Google Patents

método de produção de lipídeo através da utilização de ss-cetoacil-acp sintase

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Publication number
BR112016020517A2
BR112016020517A2 BR112016020517A BR112016020517A BR112016020517A2 BR 112016020517 A2 BR112016020517 A2 BR 112016020517A2 BR 112016020517 A BR112016020517 A BR 112016020517A BR 112016020517 A BR112016020517 A BR 112016020517A BR 112016020517 A2 BR112016020517 A2 BR 112016020517A2
Authority
BR
Brazil
Prior art keywords
acp synthase
production method
protein
ketoacyl acp
lipid production
Prior art date
Application number
BR112016020517A
Other languages
English (en)
Inventor
Endo Hiroko
Ohta Hiroyuki
Hori Koichi
Sugihara Shinji
Saito Takeshi
Tojo Takuto
Ozaki Tatsuro
Original Assignee
Kao Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kao Corp filed Critical Kao Corp
Publication of BR112016020517A2 publication Critical patent/BR112016020517A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

um método de produção de um lipídeo, que contém as etapas (1) e (2) a seguir; e um transformante obtido através da introdução de um gene que codifica a proteína (a) ou (b) a seguir em um hospedeiro: (1) introdução de um gene que codifica a proteína (a) ou (b) a seguir em um hospedeiro e dessa maneira obtendo-se um transformante e (2) coleta de um lipídeo partindo do transformante resultante: (a) uma proteína que consiste de uma sequência de aminoácidos apresentada na seq id no: 1; e (b) uma proteína que consiste de uma sequência de aminoácidos que possui 60% ou mais identidade com a sequência de aminoácidos da proteína (a) e que possui atividade de ß-cetoacil-acp sintase.
BR112016020517A 2014-03-03 2015-02-23 método de produção de lipídeo através da utilização de ss-cetoacil-acp sintase BR112016020517A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014040536 2014-03-03
PCT/JP2015/054960 WO2015133305A1 (ja) 2014-03-03 2015-02-23 β－ケトアシル－ＡＣＰシンターゼを用いた脂質の製造方法

Publications (1)

Publication Number Publication Date
BR112016020517A2 true BR112016020517A2 (pt) 2017-12-12

Family

ID=54055115

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016020517A BR112016020517A2 (pt) 2014-03-03 2015-02-23 método de produção de lipídeo através da utilização de ss-cetoacil-acp sintase

Country Status (5)

Country Link
US (1) US10066248B2 (pt)
JP (1) JP6310544B2 (pt)
AU (1) AU2015225158A1 (pt)
BR (1) BR112016020517A2 (pt)
WO (1) WO2015133305A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY180953A (en) 2012-12-27 2020-12-14 Kao Corp Acyl-acp thioesterase
AU2014288484B2 (en) 2013-07-12 2020-04-23 Kao Corporation Acyl-ACP thioesterase
AU2015277857B2 (en) 2014-06-20 2021-07-22 Kao Corporation Method for producing lipid
MY182519A (en) 2014-11-14 2021-01-25 Kao Corp Method for producing lipid using acyl-acp thioesterase
JP6568718B2 (ja) 2015-05-22 2019-08-28 花王株式会社 脂質の製造方法
US10337037B2 (en) 2015-08-05 2019-07-02 Kao Corporation Method of producing lipid
JP6587468B2 (ja) 2015-09-11 2019-10-09 花王株式会社 脂質の製造方法
JP6779652B2 (ja) 2016-04-20 2020-11-04 花王株式会社 脂質の製造方法
JP6734112B2 (ja) * 2016-05-10 2020-08-05 花王株式会社 脂質の製造方法
JP6779664B2 (ja) * 2016-05-26 2020-11-04 花王株式会社 脂質の製造方法
AU2017333801A1 (en) * 2016-09-27 2019-04-11 Kao Corporation Method of Producing Lipid
JP6971607B2 (ja) * 2016-12-19 2021-11-24 花王株式会社 脂質の製造方法
JP6934303B2 (ja) * 2017-02-08 2021-09-15 花王株式会社 脂肪族アルコールの生産方法
US11618890B2 (en) * 2018-08-22 2023-04-04 Corbion Biotech, Inc. Beta-ketoacyl-ACP synthase II variants
EP4090735A1 (en) * 2020-01-16 2022-11-23 Corbion Biotech, Inc. Beta-ketoacyl-acp synthase iv variants

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6483008B1 (en) 1990-08-15 2002-11-19 Calgene Llc Methods for producing plants with elevated oleic acid content
US5850022A (en) 1992-10-30 1998-12-15 Calgene, Inc. Production of myristate in plant cells
AR013633A1 (es) 1997-04-11 2001-01-10 Calgene Llc METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.
DE19926456A1 (de) 1999-06-10 2000-12-14 Norddeutsche Pflanzenzucht Han Verfahren zur Erhöhung des Fettsäuregehalts in Pflanzensamen
JP3477512B2 (ja) 2000-05-02 2003-12-10 独立行政法人産業技術総合研究所 微生物を用いた高度不飽和脂肪酸の製造方法
JP2002223788A (ja) 2001-01-31 2002-08-13 Kao Corp アルコールの製造方法
EP2082053B1 (en) 2006-08-01 2016-04-13 DSM Nutritional Products AG Process for producing microbial oil comprising polyunsaturated fatty acids
BR112013019699A2 (pt) * 2011-02-02 2017-12-19 Solazyme Inc método de produção de um óleo natural, óleo natural, produto, célula recombinante, célula de microrganismo, e, alimento
MY180202A (en) 2011-05-23 2020-11-24 Malaysian Palm Oil Board ?-ketoacyl acp-synthase ii (kasii) gene from jessenia bataua
US8709766B2 (en) * 2011-10-17 2014-04-29 Colorado School Of Mines Use of endogenous promoters in genetic engineering of Nannochloropsis gaditana
BR112014025719A8 (pt) 2012-04-18 2017-10-03 Solazyme Inc Óleos customizados
MY180953A (en) 2012-12-27 2020-12-14 Kao Corp Acyl-acp thioesterase
AU2014288484B2 (en) 2013-07-12 2020-04-23 Kao Corporation Acyl-ACP thioesterase
AU2015277857B2 (en) 2014-06-20 2021-07-22 Kao Corporation Method for producing lipid
US20170107545A1 (en) 2014-08-04 2017-04-20 Kao Corporation Method of Producing Medium Chain Fatty Acid Using Beta-Ketoacyl-ACP Synthase
MY182519A (en) 2014-11-14 2021-01-25 Kao Corp Method for producing lipid using acyl-acp thioesterase
US20170335353A1 (en) 2014-12-05 2017-11-23 Kao Corporation Method of Producing Lipid Using Acyl-ACP Thioesterase
JP6568718B2 (ja) 2015-05-22 2019-08-28 花王株式会社 脂質の製造方法
US10337037B2 (en) 2015-08-05 2019-07-02 Kao Corporation Method of producing lipid

Also Published As

Publication number Publication date
JPWO2015133305A1 (ja) 2017-04-06
US20170044580A1 (en) 2017-02-16
US10066248B2 (en) 2018-09-04
WO2015133305A1 (ja) 2015-09-11
AU2015225158A1 (en) 2016-09-08
JP6310544B2 (ja) 2018-04-11

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