BR112015032141A2 - Método para a produção recombinante de um polipeptídeo, e. coli e uso - Google Patents
Método para a produção recombinante de um polipeptídeo, e. coli e usoInfo
- Publication number
- BR112015032141A2 BR112015032141A2 BR112015032141A BR112015032141A BR112015032141A2 BR 112015032141 A2 BR112015032141 A2 BR 112015032141A2 BR 112015032141 A BR112015032141 A BR 112015032141A BR 112015032141 A BR112015032141 A BR 112015032141A BR 112015032141 A2 BR112015032141 A2 BR 112015032141A2
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptide
- coli
- recombinant production
- expresses
- culturing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
- C12Y106/99003—NADH dehydrogenase (1.6.99.3)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
MÉTODO PARA A PRODUÇÃO RECOMBINANTE DE UM POLIPEPTÍDEO, E. COLI E USO. No presente pedido é relatado um método para a produção recombinante de um polipeptídeo em E. coli, que compreende as etapas de i) cultivar uma E. coli deficiente em NADH desidrogenase II que expressa o polipeptídeo, e ii) recuperar o polipeptídeo a partir da célula ou do meio de cultivo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13178739 | 2013-07-31 | ||
| PCT/EP2014/066261 WO2015014829A1 (en) | 2013-07-31 | 2014-07-29 | Method for the recombinant production of a polypeptide in prokaryotic cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112015032141A2 true BR112015032141A2 (pt) | 2017-08-29 |
Family
ID=48877159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112015032141A BR112015032141A2 (pt) | 2013-07-31 | 2014-07-29 | Método para a produção recombinante de um polipeptídeo, e. coli e uso |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20160319319A1 (pt) |
| EP (1) | EP3027777A1 (pt) |
| JP (1) | JP2016524925A (pt) |
| KR (1) | KR20160034320A (pt) |
| CN (1) | CN105358705A (pt) |
| BR (1) | BR112015032141A2 (pt) |
| CA (1) | CA2915944A1 (pt) |
| HK (1) | HK1221742A1 (pt) |
| MX (1) | MX2016001187A (pt) |
| RU (1) | RU2016106950A (pt) |
| SG (1) | SG11201600725WA (pt) |
| WO (1) | WO2015014829A1 (pt) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5891669A (en) * | 1997-03-17 | 1999-04-06 | Novo Nordisk A/S, Novoalle, | Methods for producing polypeptides in respiratory-deficient cells |
| JP4380029B2 (ja) * | 2000-07-05 | 2009-12-09 | 味の素株式会社 | 微生物を利用した物質の製造法 |
| JP4551725B2 (ja) * | 2004-09-13 | 2010-09-29 | Ｏｋｉセミコンダクタ株式会社 | 半導体装置の製造方法 |
| JP2013535978A (ja) * | 2010-08-30 | 2013-09-19 | エフ．ホフマン−ラ ロシュ アーゲー | 原核生物における発現構築物 |
-
2014
- 2014-07-29 WO PCT/EP2014/066261 patent/WO2015014829A1/en active Application Filing
- 2014-07-29 KR KR1020167002566A patent/KR20160034320A/ko not_active Application Discontinuation
- 2014-07-29 CN CN201480038515.9A patent/CN105358705A/zh active Pending
- 2014-07-29 JP JP2016530491A patent/JP2016524925A/ja active Pending
- 2014-07-29 BR BR112015032141A patent/BR112015032141A2/pt not_active IP Right Cessation
- 2014-07-29 CA CA2915944A patent/CA2915944A1/en not_active Abandoned
- 2014-07-29 SG SG11201600725WA patent/SG11201600725WA/en unknown
- 2014-07-29 RU RU2016106950A patent/RU2016106950A/ru not_active Application Discontinuation
- 2014-07-29 EP EP14744841.9A patent/EP3027777A1/en not_active Withdrawn
- 2014-07-29 MX MX2016001187A patent/MX2016001187A/es unknown
-
2016
- 2016-01-28 US US15/009,588 patent/US20160319319A1/en not_active Abandoned
- 2016-08-22 HK HK16109983.2A patent/HK1221742A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SG11201600725WA (en) | 2016-02-26 |
| CN105358705A (zh) | 2016-02-24 |
| RU2016106950A (ru) | 2017-09-04 |
| WO2015014829A1 (en) | 2015-02-05 |
| MX2016001187A (es) | 2016-04-29 |
| US20160319319A1 (en) | 2016-11-03 |
| HK1221742A1 (zh) | 2017-06-09 |
| CA2915944A1 (en) | 2015-02-05 |
| EP3027777A1 (en) | 2016-06-08 |
| KR20160034320A (ko) | 2016-03-29 |
| JP2016524925A (ja) | 2016-08-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2525 DE 28-05-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |