BR112015014781A2 - célula microbiana recombinante e método de produção de um óleo microbiano - Google Patents

célula microbiana recombinante e método de produção de um óleo microbiano

Info

Publication number
BR112015014781A2
BR112015014781A2 BR112015014781A BR112015014781A BR112015014781A2 BR 112015014781 A2 BR112015014781 A2 BR 112015014781A2 BR 112015014781 A BR112015014781 A BR 112015014781A BR 112015014781 A BR112015014781 A BR 112015014781A BR 112015014781 A2 BR112015014781 A2 BR 112015014781A2
Authority
BR
Brazil
Prior art keywords
acyltransferase
cells
coa
recombinant microbial
oil
Prior art date
Application number
BR112015014781A
Other languages
English (en)
Inventor
Dauner Michael
S Yadav Narendra
L Sharpe Pamela
Qun Zhu Quinn
Hong Seung-Pyo
Fan Xiaochun
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR112015014781A2 publication Critical patent/BR112015014781A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01023—1-Acylglycerophosphocholine O-acyltransferase (2.3.1.23), i.e. lysophosphatidylcholine acyltransferase or LPCAT
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

resumo “célula microbiana recombinante e método de produção de um óleo microbiano” células microbianas recombinantes são divulgadas que produzem um óleo que compreende pelo menos 28% de ácido eicosapentaenoico (epa) medido como uma porcentagem em peso do peso de célula seca. essas células podem compreender uma sequência polinucleotídica que codifica uma acil-coa:lisofosfatidilcolina aciltransferase (lpcat) que compreende pelo menos uma mutação de aminoácido em uma porção da o-aciltransferase ligada à membrana. além disso, as células podem compreender a infrarregulação de uma sequência polinucleotídica endógena que codifica a proteína de utilização do sorbitol sou2 e/ou um ou mais polinucleotídeos que codificam a fosfolipídio:diacilglicerol aciltransferase (pdat), a delta-12 dessaturase, uma multienzima do ácido di-homo-gama-linolênico sintase (dgla), a delta-8 dessaturase, malonil-coa sintetase (mcs) ou acil-coa:ácido lisofosfatídico aciltransferase (lpaat). também são divulgados métodos de uso das células microbianas recombinantes para produzir óleos contendo ácidos graxos ômega-3 poli-insaturados, como o epa.
BR112015014781A 2012-12-21 2013-12-18 célula microbiana recombinante e método de produção de um óleo microbiano BR112015014781A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261740502P 2012-12-21 2012-12-21
PCT/US2013/075896 WO2014100062A1 (en) 2012-12-21 2013-12-18 Recombinant microbial cells that produce at least 28% eicosapentaenoic acid as dry cell weight

Publications (1)

Publication Number Publication Date
BR112015014781A2 true BR112015014781A2 (pt) 2017-10-10

Family

ID=49956389

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015014781A BR112015014781A2 (pt) 2012-12-21 2013-12-18 célula microbiana recombinante e método de produção de um óleo microbiano

Country Status (9)

Country Link
US (1) US9701989B2 (pt)
EP (1) EP2935601B1 (pt)
JP (1) JP2016502852A (pt)
BR (1) BR112015014781A2 (pt)
CA (1) CA2893409A1 (pt)
CL (1) CL2015001673A1 (pt)
DK (1) DK2935601T3 (pt)
NO (1) NO2976143T3 (pt)
WO (1) WO2014100062A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
EP3597740B1 (en) 2014-11-06 2022-03-23 DuPont US Holding, LLC Peptide-mediated delivery of rna-guided endonuclease into cells
EP4144844A1 (en) 2015-10-12 2023-03-08 DuPont US Holding, LLC Protected dna templates for gene modification and increased homologous recombination in cells and methods of use
CN108330114B (zh) * 2018-03-21 2020-11-03 北京大学 一种利用epa的甘油二酯酰基转移酶及其应用
KR102536297B1 (ko) * 2021-02-08 2023-05-24 세종대학교산학협력단 식물체의 오메가 3 지방산 함량 증가 방법

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2517253C (en) 2003-02-27 2018-07-03 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
EP1613746B1 (de) * 2003-03-31 2013-03-06 University Of Bristol Neue pflanzliche acyltransferasen spezifisch für langkettige, mehrfach ungesättigte fettsäuren
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US20110059496A1 (en) 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
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US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) * 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
US7732155B2 (en) 2006-12-13 2010-06-08 National Research Council Of Canada Methods for identifying lysophosphatidylcholine acyltransferases
US20090117253A1 (en) 2007-10-03 2009-05-07 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms
DK2676554T3 (da) * 2009-03-26 2015-02-09 Suntory Holdings Ltd Ny lysophospholipidacyltransferase
EP2821492A3 (en) * 2009-05-13 2015-04-08 BASF Plant Science Company GmbH Acyltransferases and uses thereof in fatty acid production
US8399226B2 (en) 2009-06-16 2013-03-19 E I Du Pont De Nemours And Company High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica
AU2010333827A1 (en) * 2009-12-24 2012-06-28 E. I. Du Pont De Nemours And Company Plant membrane bound O-acyl transferase (MBOAT) family protein sequences and their uses for altering fatty acid compositions
EP2585603B1 (en) * 2010-06-25 2017-12-20 BASF Plant Science Company GmbH Acyltransferases and uses therof in fatty acid production
EP2609203B1 (en) 2010-08-26 2018-06-20 E. I. du Pont de Nemours and Company Recombinant microbial host cells for high eicosapentaenoic acid production
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US8906650B2 (en) 2011-04-01 2014-12-09 E I Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
US8735094B2 (en) 2011-04-05 2014-05-27 E I Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast
EP2702148A1 (en) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
US8816111B2 (en) * 2012-06-15 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
BR112014031784A2 (pt) * 2012-06-19 2019-10-01 Du Pont acil-coa:lisofosfatidilcolina aciltransferases mutantes
US9005940B2 (en) * 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells

Also Published As

Publication number Publication date
EP2935601B1 (en) 2018-03-21
CA2893409A1 (en) 2014-06-26
EP2935601A1 (en) 2015-10-28
CL2015001673A1 (es) 2015-10-23
US9701989B2 (en) 2017-07-11
DK2935601T3 (en) 2018-06-18
NO2976143T3 (pt) 2018-08-04
JP2016502852A (ja) 2016-02-01
US20140186906A1 (en) 2014-07-03
WO2014100062A1 (en) 2014-06-26

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements