BR112014032560A2 - células para produzir iduronato-2-sulfatase recombinante - Google Patents

células para produzir iduronato-2-sulfatase recombinante

Info

Publication number
BR112014032560A2
BR112014032560A2 BR112014032560-0A BR112014032560A BR112014032560A2 BR 112014032560 A2 BR112014032560 A2 BR 112014032560A2 BR 112014032560 A BR112014032560 A BR 112014032560A BR 112014032560 A2 BR112014032560 A2 BR 112014032560A2
Authority
BR
Brazil
Prior art keywords
cells
present
recombinant
adaptable
protein
Prior art date
Application number
BR112014032560-0A
Other languages
English (en)
Other versions
BR112014032560B1 (pt
Inventor
Bolgog Ferenc
Heartlein Mike
Original Assignee
Shire Human Genetic Therapies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shire Human Genetic Therapies, Inc. filed Critical Shire Human Genetic Therapies, Inc.
Publication of BR112014032560A2 publication Critical patent/BR112014032560A2/pt
Publication of BR112014032560B1 publication Critical patent/BR112014032560B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/99—Oxidoreductases acting on sulfur groups as donors (1.8) with other acceptors (1.8.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06013—Iduronate-2-sulfatase (3.1.6.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

abstract the present invention provides, among other things, methods and compositions for production of recombinant i2s protein with improved potency and activity using cells co-express i2s and fge protein. in some embodiments, cells according to the present invention are engineered to simultaneously over-express recombinant i2s and fge proteins. cells according to the invention are adaptable to various cell culture conditions. in some embodiments, cells of the present invention adaptable to a large-scale suspension serum-free culture. tradução do resumo resumo "células para produzir iduronato-2-sulfatase recombinante" a presente invenção fornece, entre outras coisas, métodos e composições para produção de proteína i2s recombinante com potência e atividade melhoradas usando células que coexpressam proteína i2s e fge. em algumas modalidades, as células de acordo com a presente invenção são engenheiradas para simultaneamente superexpressarem proteínas i2s e fge recombinantes. as células de acordo com a invenção são adaptáveis a diversas condições de cultura de célula. em algumas modalidades, as células da presente invenção são adaptáveis a uma cultura livre de soro de suspensão em grande escala.
BR112014032560-0A 2012-06-29 2013-06-28 Método para produzir a proteína iduronato-2-sulfatase (i2s) recombinante BR112014032560B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261666719P 2012-06-29 2012-06-29
US61/666,719 2012-06-29
PCT/US2013/048571 WO2014005019A2 (en) 2012-06-29 2013-06-28 Cells for producing recombinant iduronate-2-sulfatase

Publications (2)

Publication Number Publication Date
BR112014032560A2 true BR112014032560A2 (pt) 2018-03-06
BR112014032560B1 BR112014032560B1 (pt) 2022-04-26

Family

ID=49778522

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014032560-0A BR112014032560B1 (pt) 2012-06-29 2013-06-28 Método para produzir a proteína iduronato-2-sulfatase (i2s) recombinante

Country Status (27)

Country Link
US (3) US9150841B2 (pt)
EP (1) EP2874649B1 (pt)
JP (3) JP6067849B2 (pt)
KR (1) KR101710487B1 (pt)
CN (2) CN104540517A (pt)
AU (3) AU2013282400B2 (pt)
BR (1) BR112014032560B1 (pt)
CA (1) CA2877521C (pt)
CL (1) CL2014003568A1 (pt)
CO (1) CO7240394A2 (pt)
CR (1) CR20140586A (pt)
DO (1) DOP2014000298A (pt)
EA (2) EA035511B1 (pt)
ES (1) ES2718346T3 (pt)
GT (1) GT201400302A (pt)
HK (2) HK1209331A1 (pt)
IL (3) IL236343A (pt)
MX (2) MX336716B (pt)
MY (2) MY157086A (pt)
NZ (2) NZ703137A (pt)
PE (1) PE20150957A1 (pt)
PH (2) PH12014502868B1 (pt)
SG (2) SG10201509397SA (pt)
TR (1) TR201904435T4 (pt)
UA (1) UA118955C2 (pt)
WO (1) WO2014005019A2 (pt)
ZA (1) ZA201409396B (pt)

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Also Published As

Publication number Publication date
ZA201409396B (en) 2018-05-30
EP2874649A4 (en) 2016-05-04
US9150841B2 (en) 2015-10-06
CN104540517A (zh) 2015-04-22
AU2016200460A1 (en) 2016-02-18
EA201492183A1 (ru) 2015-06-30
NZ703137A (en) 2017-08-25
AU2013282400A1 (en) 2015-01-29
IL274266B (en) 2021-04-29
US20160053238A1 (en) 2016-02-25
JP2016105737A (ja) 2016-06-16
KR101710487B1 (ko) 2017-02-27
EA202090805A1 (ru) 2020-11-30
AU2013282400B2 (en) 2016-02-04
BR112014032560B1 (pt) 2022-04-26
IL236343A (en) 2017-10-31
AU2018200710A1 (en) 2018-02-22
SG10201509397SA (en) 2015-12-30
WO2014005019A2 (en) 2014-01-03
MX2015000189A (es) 2015-04-08
CA2877521C (en) 2021-06-01
DOP2014000298A (es) 2015-03-31
IL254560B (en) 2020-05-31
JP6755272B2 (ja) 2020-09-16
WO2014005019A3 (en) 2014-02-27
KR20150030242A (ko) 2015-03-19
UA118955C2 (uk) 2019-04-10
MY189314A (en) 2022-02-04
TR201904435T4 (tr) 2019-05-21
ES2718346T3 (es) 2019-07-01
HK1209331A1 (en) 2016-04-01
GT201400302A (es) 2019-05-22
US20180010108A1 (en) 2018-01-11
AU2018200710B2 (en) 2019-11-14
HK1209651A1 (en) 2016-04-08
JP2015521848A (ja) 2015-08-03
US9719074B2 (en) 2017-08-01
MY157086A (en) 2016-04-19
SG11201408702VA (en) 2015-02-27
IL274266A (en) 2020-06-30
JP6067849B2 (ja) 2017-01-25
MX366155B (es) 2019-06-28
PE20150957A1 (es) 2015-07-15
US20140004593A1 (en) 2014-01-02
CA2877521A1 (en) 2014-01-03
CR20140586A (es) 2015-04-06
JP6302499B2 (ja) 2018-03-28
IL236343A0 (en) 2015-02-26
JP2018088933A (ja) 2018-06-14
PH12014502868A1 (en) 2015-02-23
PH12014502868B1 (en) 2015-02-23
AU2016200460B2 (en) 2017-11-02
CN107267460A (zh) 2017-10-20
NZ734670A (en) 2019-12-20
PH12016500011A1 (en) 2019-08-14
EA035511B1 (ru) 2020-06-26
CO7240394A2 (es) 2015-04-17
EP2874649A2 (en) 2015-05-27
EP2874649B1 (en) 2019-01-02
MX336716B (es) 2016-01-28
IL254560A0 (en) 2017-11-30
CL2014003568A1 (es) 2015-04-24

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