BR112014019168A8 - Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos - Google Patents

Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos

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BR112014019168A8
BR112014019168A8 BR112014019168A BR112014019168A BR112014019168A8 BR 112014019168 A8 BR112014019168 A8 BR 112014019168A8 BR 112014019168 A BR112014019168 A BR 112014019168A BR 112014019168 A BR112014019168 A BR 112014019168A BR 112014019168 A8 BR112014019168 A8 BR 112014019168A8
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analytes
absence
detecting
analyte
sample volume
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BR112014019168A
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BR112014019168B1 (pt
BR112014019168A2 (pt
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P Kartalov Emil
Rajagopal Aditya
Scherer Axel
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California Inst Of Techn
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    • C12Q1/6844Nucleic acid amplification reactions
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    • G01MEASURING; TESTING
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    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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Abstract

ENSAIO CAPAZ DE DETECTAR DE FORMA NÃO DEGENERADA PRESENÇA OU AUSÊNCIA DE ANALITOS EM UM ÚNICO VOLUME DE AMOSTRA, BEM COMO MÉTODO DE DETECÇÃO DA PRESENÇA OU AUSÊNCIA DE ANALITO DENTRE UMA PLURALIDADE DOS MESMOS. A presente invenção refere-se a um ensaio capaz de detectar de forma não degenerada a presença ou ausência de cada um dentre ao menos sete (7) analitos, em qualquer combinação de presença ou ausência, em um único volume de amostra sem imobilização dos ditos analitos, separação física dos ditos analitos, análise de curva de fusão ou espectrometria de massa. A presente invenção refere-se ainda a um método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos.
BR112014019168-9A 2012-02-03 2013-02-01 Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos BR112014019168B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261594480P 2012-02-03 2012-02-03
US61/594,480 2012-02-03
US201261703093P 2012-09-19 2012-09-19
US61/703,093 2012-09-19
PCT/US2013/024509 WO2013116780A1 (en) 2012-02-03 2013-02-01 Signal encoding and decoding in multiplexed biochemical assays

Publications (3)

Publication Number Publication Date
BR112014019168A8 true BR112014019168A8 (pt) 2017-07-11
BR112014019168A2 BR112014019168A2 (pt) 2021-06-01
BR112014019168B1 BR112014019168B1 (pt) 2021-10-05

Family

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BR112014019168-9A BR112014019168B1 (pt) 2012-02-03 2013-02-01 Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos

Country Status (8)

Country Link
US (9) US8838394B2 (pt)
EP (6) EP4361609A3 (pt)
JP (1) JP6255353B2 (pt)
KR (2) KR102145677B1 (pt)
CN (3) CN114717296A (pt)
BR (1) BR112014019168B1 (pt)
HK (1) HK1205200A1 (pt)
WO (1) WO2013116780A1 (pt)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4361609A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems
CA2897474A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
KR101789079B1 (ko) 2014-09-22 2017-10-24 건국대학교 산학협력단 마이크로알엔에이 검지를 위한 핵산 다중 접합체 및 그 제조방법
WO2016120396A1 (en) * 2015-01-29 2016-08-04 Instituut Voor Tropische Geneeskunde Combined plasmodium-rna virus detection method
WO2016161325A1 (en) * 2015-04-02 2016-10-06 University Of Washington Optical painting and fluorescence-activated sorting of adherent cells
CN108351352B (zh) * 2015-10-05 2021-09-10 北卡罗来纳-查佩尔山大学 用于多路测定的解码方法及相关流体设备、试剂盒和固体支持物
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
WO2017209563A1 (en) * 2016-06-02 2017-12-07 Seegene, Inc. . Method for detecting a target analyte in a sample using a signal change-amount data set
EP3472354A4 (en) 2016-06-17 2020-01-01 California Institute of Technology NUCLEIC ACID ACTIONS AND RELATED PROCEDURES AND COMPOSITIONS
JP6846636B2 (ja) * 2017-02-28 2021-03-24 パナソニックIpマネジメント株式会社 核酸検出用オリゴヌクレオチド
WO2019006023A1 (en) * 2017-06-28 2019-01-03 ChromaCode, Inc. MULTIPLEXED FLUORIMETRIC MEASUREMENTS WITH DROPLET PCR SYSTEMS
JP2019154246A (ja) * 2018-03-08 2019-09-19 株式会社リコー プローブセット、及びそれを用いた核酸検出方法
WO2019236447A1 (en) * 2018-06-04 2019-12-12 ChromaCode, Inc. Methods for watermarking and identifying chemical compositions
US20200010876A1 (en) * 2018-07-03 2020-01-09 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
EP3821034A4 (en) * 2018-07-11 2022-04-27 Chromacode, Inc. METHODS FOR QUANTIFYING ANALYTES IN MULTIPLEXED BIOCHEMICAL REACTIONS
USD975312S1 (en) 2020-02-14 2023-01-10 Beckman Coulter, Inc. Reagent cartridge

Family Cites Families (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5079351A (en) 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DK0519338T3 (da) 1991-06-20 1996-10-28 Hoffmann La Roche Forbedrede fremgangsmåder til nukleinsyreamplifikation
CA2130562A1 (en) 1992-02-19 1993-09-02 Alexander B. Chetverin Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5948360A (en) * 1994-07-11 1999-09-07 Tekmar Company Autosampler with robot arm
AU709045B2 (en) 1995-02-02 1999-08-19 Chugai Seiyaku Kabushiki Kaisha Method for assay of analyte by adjustment of chemiluminescence
CA2216645A1 (en) * 1995-04-25 1996-11-21 Irori Remotely programmable matrices with memories and uses thereof
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US20050112637A1 (en) 1995-09-08 2005-05-26 Chatterjee Deb K. Mutant DNA polymerases and uses thereof
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6007994A (en) 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
WO1997035589A1 (en) 1996-03-26 1997-10-02 Kopreski Michael S Method enabling use of extracellular rna extracted from plasma or serum to detect, monitor or evaluate cancer
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5834203A (en) 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
AU1080999A (en) 1997-10-14 1999-05-03 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU3555599A (en) 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
AU762728B2 (en) 1998-07-02 2003-07-03 Gen-Probe Incorporated Molecular torches
US6642062B2 (en) 1998-09-03 2003-11-04 Trellis Bioinformatics, Inc. Multihued labels
EP1171636A2 (en) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
DE60035691T2 (de) 1999-07-23 2008-04-30 Forensic Science Service Ltd. Verfahren zur Erkennung von Einzel-Nukleotid-Polymorphismen
ATE336591T1 (de) 1999-10-22 2006-09-15 New York Health Res Inst Nachweisverfahren für kurze sequenzvarianten
US20040053230A1 (en) 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
EP1278889B1 (en) 2000-03-29 2006-07-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
WO2002004680A2 (en) * 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
US7608683B2 (en) * 2000-08-09 2009-10-27 The Regents Of The University Of California Stress proteins and peptides and methods of use thereof
CA2314398A1 (en) * 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
US20020146734A1 (en) * 2001-02-21 2002-10-10 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
CA2439402A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
WO2009003492A1 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
AU2002322457A1 (en) * 2001-06-28 2003-03-03 Illumina, Inc. Multiplex decoding of array sensors with microspheres
AU2002322348A1 (en) * 2001-06-28 2003-03-03 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP1288308A1 (en) 2001-08-28 2003-03-05 Roche Diagnostics GmbH A method for the determination of multiple analytes
US6852491B2 (en) 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US20030134320A1 (en) 2002-01-15 2003-07-17 Myriad Genetics, Incorporated Method system and computer program product for quality assurance in detecting biochemical markers
EP1426448A1 (en) 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
EP2284278A1 (en) * 2003-04-04 2011-02-16 Roche Diagnostics GmbH Improved system for multi color real time PCR
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US7070786B2 (en) 2003-06-06 2006-07-04 Centocor, Inc. RSV proteins, antibodies, compositions, methods and uses
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
TWI232934B (en) 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
WO2005051967A2 (en) 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
US7601821B2 (en) * 2004-03-24 2009-10-13 Applied Biosystems, Llc Encoding and decoding reactions for determining target molecules
EP1740711A2 (en) 2004-04-01 2007-01-10 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
ES2527426T3 (es) 2004-05-07 2015-01-23 Cepheid Detección combinada de agentes biológicos
US7575864B2 (en) 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
DE102004043870B4 (de) 2004-09-10 2009-11-19 Eppendorf Ag Verfahren zur Erweiterung des dynamischen Erfassungsbereich bei Mikroarrays
AU2005287517A1 (en) 2004-09-24 2006-03-30 Universitaet Bern Molecular beacons
ES2529204T3 (es) 2004-10-18 2015-02-17 Brandeis University Métodos para la amplificación de ácido nucleico
WO2006047777A2 (en) 2004-10-27 2006-05-04 Cepheid Closed-system multi-stage nucleic acid amplification reactions
CA2595729A1 (en) 2005-01-21 2006-07-27 Third Wave Technologies, Inc. Methods and compositions for increased dynamic range detection of nucleic acid molecules
US8168602B2 (en) * 2005-02-17 2012-05-01 Dana-Farber Cancer Institute, Inc. Compositions, kits and methods for identification, assessment, prevention and therapy of cancer
US7709249B2 (en) 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7919244B2 (en) 2005-06-16 2011-04-05 Biotools Biotechnological & Medical Laboratories, S.A. Nucleic acid detection method involving the direct generation of a measurable signal
US8067208B2 (en) 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
PL1937834T3 (pl) 2005-09-01 2014-11-28 Mtpcr Pty Ltd Sposoby wzmacniania, kwantyfikacji i identyfikacji kwasów nukleinowych
US7553955B2 (en) 2005-09-01 2009-06-30 The Trustees Of The University Of Pennsylvania Molecular beacons, methods and kits for detecting DNA damage response
CN1936019B (zh) * 2005-10-18 2013-01-30 厦门大学 用于多重实时核酸扩增检测的探针编码方法
AU2006330834B2 (en) 2005-12-23 2013-09-12 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
ES2374788T3 (es) 2005-12-23 2012-02-22 Nanostring Technologies, Inc. Nanoinformadores y métodos para su producción y uso.
WO2007080583A2 (en) 2006-01-10 2007-07-19 Applied Spectral Imaging Ltd. Methods and systems for analyzing biological samples
US8455184B2 (en) 2006-04-18 2013-06-04 The United States Of America As Represented By The Secretary Of The Air Force Differential multiplexing with pattern recognition
WO2007121489A2 (en) * 2006-04-19 2007-10-25 Applera Corporation Reagents, methods, and libraries for gel-free bead-based sequencing
US7941279B2 (en) 2006-05-22 2011-05-10 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
EP2210955A3 (en) 2006-05-23 2010-09-15 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
WO2008005248A2 (en) * 2006-06-30 2008-01-10 Canon U.S. Life Sciences, Inc. Real-time pcr in micro-channels
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
WO2008036614A1 (en) * 2006-09-18 2008-03-27 California Institute Of Technology Apparatus for detecting target molecules and related methods
US8993256B2 (en) 2006-09-28 2015-03-31 The Regents Of The University Of California Fusion proteins and polynucleotide constructs to measure protein turn-over
EP2061901A2 (en) 2006-10-31 2009-05-27 Noxxon Pharma AG Methods for detection of a single- or double-stranded nucleic acid molecule
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
EP2102773B1 (en) 2006-11-30 2012-08-01 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
AU2007333040B2 (en) 2006-12-13 2013-02-07 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
CA2580589C (en) * 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US20090042735A1 (en) 2007-03-05 2009-02-12 Blair Steven M Methods and Compositions Related to Nucleic Acid Detection
WO2008118998A2 (en) 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
EP2155899A2 (en) 2007-04-10 2010-02-24 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2009027102A2 (en) 2007-08-29 2009-03-05 Eppendorf Ag Device and method for radiometric measurement of a plurality of samples
EP2195453A4 (en) 2007-09-20 2011-02-02 Molecular Plant Breeding Nominees Ltd METHOD FOR AMPLIFYING NUCLEIC ACID
CN101646786A (zh) 2007-12-26 2010-02-10 爱科来株式会社 目标核酸序列的扩增方法和用于该方法的探针
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
SG182951A1 (en) * 2008-06-25 2012-08-30 Baylor Res Inst Blood transcriptional signature of mycobacterium tuberculosis infection
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
CA2733186A1 (en) 2008-08-08 2010-02-11 Smiths Detection Inc. Detection algorithm for pcr assay
JP5836803B2 (ja) 2008-08-14 2015-12-24 ナノストリング テクノロジーズ, インコーポレイテッド 安定したナノレポーター
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
CN102232118B (zh) 2008-12-01 2015-07-08 恰根有限公司 用于核酸检测的新型荧光染料组合物
EP2376659B1 (en) 2008-12-17 2015-12-02 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US8361738B2 (en) 2009-03-06 2013-01-29 California Institute Of Technology Methods for quantitative target detection and related devices and systems
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101570782B (zh) * 2009-03-20 2012-01-11 曹际娟 乳制品中8种致病菌检测试剂盒及其检测方法
FI20095514A0 (fi) 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
WO2010130877A2 (en) 2009-05-12 2010-11-18 Abacus Diagnostica Oy Method for detecting nucleic acids
CN102449167B (zh) 2009-05-26 2014-12-17 厦门大学 一种检测核酸序列变异的方法
EP2439283B1 (en) 2009-05-26 2014-12-31 Xiamen University Method for the detection of multiple single nucleotide variations or single nucleotide polymorphisms in a single tube
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
US9938569B2 (en) 2009-09-10 2018-04-10 Diasorin S.P.A. Compensation for spectral crosstalk in multiplex nucleic acid amplification
WO2011037306A1 (en) 2009-09-24 2011-03-31 Seegene, Inc. Detection of target nucleic acid sequences by cyclic exonucleolytic reactions
US8962251B2 (en) 2009-10-08 2015-02-24 Medical Service Consultation International, Llc Methods and compositions for identifying sulfur and iron modifying bacteria
CA2775613C (en) 2009-10-13 2018-06-12 Nanostring Technologies, Inc. Protein detection via nanoreporters
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
US9220743B2 (en) 2010-01-22 2015-12-29 Nuovo Biologics, Llc Pan-antiviral peptides for protein kinase inhibition
AU2011215753B2 (en) 2010-02-11 2015-09-03 Bruker Spatial Biology, Inc. Compositions and methods for the detection of small RNAs
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3392349A1 (en) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digital analyte analysis
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
CA2799200A1 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
US9822397B2 (en) 2010-06-30 2017-11-21 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
CN107502660B (zh) 2011-01-20 2021-08-10 华盛顿大学商业中心 进行数字测量的方法和***
US20150140554A1 (en) 2011-02-03 2015-05-21 Richard O. Snyder Detection Methods for Target DNA
US9493825B2 (en) 2011-03-18 2016-11-15 University Of South Florida Materials and methods for profiling microRNAs
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
WO2012135340A2 (en) 2011-03-28 2012-10-04 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
AU2012272763B2 (en) 2011-06-24 2016-11-03 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8858623B2 (en) 2011-11-04 2014-10-14 Valtech Cardio, Ltd. Implant having multiple rotational assemblies
EP4249605A3 (en) * 2011-12-22 2023-11-15 President And Fellows Of Harvard College Methods for analyte detection
EP4361609A3 (en) 2012-02-03 2024-07-17 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
BR112014032677A2 (pt) 2012-06-27 2019-01-15 Univ Rutgers ensaios rápido para ativação de célula t por medições de rna usando citometria de fluxo
EP2880184B1 (en) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
CA2897474A1 (en) 2013-01-24 2014-07-31 California Institute Of Technology Chromophore-based characterization and detection methods
WO2014165210A2 (en) 2013-03-12 2014-10-09 Life Technologies Corporation Universal reporter-based genotyping methods and materials
EP2873740A1 (en) 2013-11-19 2015-05-20 Oncodiag Methods for the surveillance, diagnosis and screening of bladder cancer
US9670530B2 (en) 2014-01-30 2017-06-06 Illumina, Inc. Haplotype resolved genome sequencing
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
MA39951A (fr) 2014-05-09 2017-03-15 Lifecodexx Ag Détection de l'adn provenant d'un type spécifique de cellule et méthodes associées
ES2954456T3 (es) 2015-03-25 2023-11-22 Massachusetts Gen Hospital Análisis digital de células tumorales circulantes en muestras de sangre
CN114196746A (zh) 2016-02-10 2022-03-18 密歇根大学董事会 核酸的检测
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
EP3472354A4 (en) 2016-06-17 2020-01-01 California Institute of Technology NUCLEIC ACID ACTIONS AND RELATED PROCEDURES AND COMPOSITIONS
AU2017348009B2 (en) 2016-10-24 2019-10-10 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
WO2019006023A1 (en) 2017-06-28 2019-01-03 ChromaCode, Inc. MULTIPLEXED FLUORIMETRIC MEASUREMENTS WITH DROPLET PCR SYSTEMS
US20190112636A1 (en) 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
WO2019169043A1 (en) 2018-02-28 2019-09-06 ChromaCode, Inc. Molecular targets for fetal nucleic acid analysis
US20200010876A1 (en) 2018-07-03 2020-01-09 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

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