BR0214489A - Processo para a produção de um polipeptìdeo recombinante - Google Patents
Processo para a produção de um polipeptìdeo recombinanteInfo
- Publication number
- BR0214489A BR0214489A BR0214489-1A BR0214489A BR0214489A BR 0214489 A BR0214489 A BR 0214489A BR 0214489 A BR0214489 A BR 0214489A BR 0214489 A BR0214489 A BR 0214489A
- Authority
- BR
- Brazil
- Prior art keywords
- nucleotide sequence
- recombinant polypeptide
- polynucleotide vector
- host cell
- sequence encoding
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2121/00—Preparations for use in therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
"PROCESSO PARA A PRODUçãO DE UM POLIPEPTìDEO RECOMBINANTE". A presente invenção refere-se a um processo para a produção de uma célula hospedeira eucariótica transformada que expressa um polipeptídeo recombinante de interesse cujo processo compreende introduzir em uma célula hospedeira eucariótica: (a) um primeiro vetor de polinucleotídeo que compreende (i) uma primeira seq³ência de nucleotídeo que codifica um polipeptídeo recombinante de interesse e (ii) uma segunda seq³ência de nucleotídeo que codifica um marcador selecionável, cuja segunda seq³ência de nucleotídeo é amplificada quando a célula hospedeira está em contato com um agente de seleção e (b) um segundo vetor de polinucleotídeo que tem essencialmente a mesma seq³ência de nucleotídeo que o primeiro vetor de polinucleotídeo exceto que a segunda seq³ência de nucleotídeo é substituída por uma terceira seq³ência de nucleotídeo que codifica um marcador selecionável diferente, o primeiro vetor de polinucleotídeo e o segundo vetor de polinucleotídeo estando estavelmente integrados ao genoma da célula hospedeira.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33386801P | 2001-11-28 | 2001-11-28 | |
PCT/EP2002/013297 WO2003046187A1 (en) | 2001-11-28 | 2002-11-26 | Method for producing a recombinant polypeptide |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0214489A true BR0214489A (pt) | 2004-10-19 |
Family
ID=23304597
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0214489-1A BR0214489A (pt) | 2001-11-28 | 2002-11-26 | Processo para a produção de um polipeptìdeo recombinante |
Country Status (19)
Country | Link |
---|---|
US (1) | US20050084969A1 (pt) |
EP (1) | EP1453966B1 (pt) |
JP (1) | JP4723185B2 (pt) |
KR (1) | KR20040063937A (pt) |
CN (1) | CN1596310A (pt) |
AU (1) | AU2002358047A1 (pt) |
BR (1) | BR0214489A (pt) |
CA (1) | CA2468832A1 (pt) |
HR (1) | HRP20040477A2 (pt) |
HU (1) | HUP0500059A2 (pt) |
IL (1) | IL161909A0 (pt) |
MX (1) | MXPA04005191A (pt) |
NO (1) | NO20042173L (pt) |
NZ (1) | NZ533133A (pt) |
PL (1) | PL370550A1 (pt) |
RU (1) | RU2004119814A (pt) |
SI (1) | SI1453966T1 (pt) |
WO (1) | WO2003046187A1 (pt) |
ZA (1) | ZA200403802B (pt) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005033135A1 (en) * | 2003-10-09 | 2005-04-14 | Daewoong Co., Ltd. | Process for purifying human thrombopoietin with high content of sialic acid |
AR053416A1 (es) | 2005-11-10 | 2007-05-09 | Protech Pharma S A | Combinacion de glicoisoformas para el tratamiento o prevencion de la septicemia, linea celular transgenica productora de glicoformas de eritropoyetina, composicion farmaceutica que comprende a dicha combinacion, procedimientos para obtener la linea celular, procedimiento para producir dicha combinac |
TR201816045T4 (tr) | 2007-10-12 | 2018-11-21 | Hoffmann La Roche | Birden fazla nükleik asitten protein ekspresyonu. |
WO2012077128A1 (en) * | 2010-12-07 | 2012-06-14 | Intas Biopharmaceuticals Limited | A novel cell line development process to produce recombinant proteins using twin vector expression system |
EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
US9505833B2 (en) | 2012-04-20 | 2016-11-29 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
US10066001B2 (en) | 2013-03-15 | 2018-09-04 | Apotex Inc. | Enhanced liquid formulation stability of erythropoietin alpha through purification processing |
JP6050289B2 (ja) * | 2013-07-11 | 2016-12-21 | Jcrファーマ株式会社 | 組換え蛋白質高発現細胞株の選択法 |
EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
WO2018162517A1 (en) | 2017-03-10 | 2018-09-13 | F. Hoffmann-La Roche Ag | Method for producing multispecific antibodies |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965199A (en) * | 1984-04-20 | 1990-10-23 | Genentech, Inc. | Preparation of functional human factor VIII in mammalian cells using methotrexate based selection |
IL74909A (en) * | 1984-04-20 | 1992-01-15 | Genentech Inc | Preparation of functional human factor viii and dna sequences,expression vectors,transformed microorganisms and cell lines used therein |
DE3624453A1 (de) * | 1986-07-19 | 1988-01-28 | Behringwerke Ag | Verfahren zur herstellung von humanem antithrombin iii (atiii), dafuer geeignete vektoren und wirtszellen, so erhaltenes, biologisch aktives atiii und dieses enthaltende arzneimittel |
EP0319206A3 (en) * | 1987-11-30 | 1990-04-18 | Berlex Laboratories, Inc. | Gene amplification |
IL118201A (en) * | 1995-05-11 | 2004-12-15 | Roche Diagnostics Gmbh | Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof |
-
2002
- 2002-11-26 BR BR0214489-1A patent/BR0214489A/pt not_active IP Right Cessation
- 2002-11-26 PL PL02370550A patent/PL370550A1/xx not_active Application Discontinuation
- 2002-11-26 CA CA002468832A patent/CA2468832A1/en not_active Abandoned
- 2002-11-26 RU RU2004119814/13A patent/RU2004119814A/ru not_active Application Discontinuation
- 2002-11-26 SI SI200231005T patent/SI1453966T1/sl unknown
- 2002-11-26 WO PCT/EP2002/013297 patent/WO2003046187A1/en active Application Filing
- 2002-11-26 HU HU0500059A patent/HUP0500059A2/hu unknown
- 2002-11-26 JP JP2003547619A patent/JP4723185B2/ja not_active Expired - Lifetime
- 2002-11-26 IL IL16190902A patent/IL161909A0/xx unknown
- 2002-11-26 NZ NZ533133A patent/NZ533133A/en unknown
- 2002-11-26 CN CNA028236963A patent/CN1596310A/zh active Pending
- 2002-11-26 MX MXPA04005191A patent/MXPA04005191A/es not_active Application Discontinuation
- 2002-11-26 AU AU2002358047A patent/AU2002358047A1/en not_active Abandoned
- 2002-11-26 KR KR10-2004-7008030A patent/KR20040063937A/ko not_active Application Discontinuation
- 2002-11-26 EP EP20020791720 patent/EP1453966B1/en not_active Revoked
- 2002-11-26 US US10/497,015 patent/US20050084969A1/en not_active Abandoned
-
2004
- 2004-05-18 ZA ZA200403802A patent/ZA200403802B/en unknown
- 2004-05-26 NO NO20042173A patent/NO20042173L/no not_active Application Discontinuation
- 2004-05-27 HR HR20040477A patent/HRP20040477A2/xx not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
RU2004119814A (ru) | 2005-04-20 |
EP1453966A1 (en) | 2004-09-08 |
KR20040063937A (ko) | 2004-07-14 |
MXPA04005191A (es) | 2005-04-29 |
CN1596310A (zh) | 2005-03-16 |
AU2002358047A1 (en) | 2003-06-10 |
US20050084969A1 (en) | 2005-04-21 |
NO20042173L (no) | 2004-08-11 |
JP4723185B2 (ja) | 2011-07-13 |
HRP20040477A2 (en) | 2005-06-30 |
ZA200403802B (en) | 2005-07-01 |
PL370550A1 (en) | 2005-05-30 |
EP1453966B1 (en) | 2012-07-11 |
NZ533133A (en) | 2006-02-24 |
SI1453966T1 (sl) | 2012-11-30 |
HUP0500059A2 (hu) | 2005-04-28 |
JP2005510242A (ja) | 2005-04-21 |
CA2468832A1 (en) | 2003-06-05 |
IL161909A0 (en) | 2005-11-20 |
WO2003046187A1 (en) | 2003-06-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A E ,7A ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 2064 DE 27/07/2010. |