AU6754196A - Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease - Google Patents

Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease

Info

Publication number
AU6754196A
AU6754196A AU67541/96A AU6754196A AU6754196A AU 6754196 A AU6754196 A AU 6754196A AU 67541/96 A AU67541/96 A AU 67541/96A AU 6754196 A AU6754196 A AU 6754196A AU 6754196 A AU6754196 A AU 6754196A
Authority
AU
Australia
Prior art keywords
hepatitis
virus
serine protease
fluorescent substrate
novel fluorescent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU67541/96A
Inventor
Yasuhiko Masuho
Yasuaki Shimizu
Kunitada Shimotohno
Kayo Yamaji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Astellas Pharma Inc
Original Assignee
RATIONAL DRUG DESIGN LAB
Astellas Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RATIONAL DRUG DESIGN LAB, Astellas Pharma Inc filed Critical RATIONAL DRUG DESIGN LAB
Publication of AU6754196A publication Critical patent/AU6754196A/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18Testing for antimicrobial activity of a material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/18Togaviridae; Flaviviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU67541/96A 1995-08-25 1996-08-22 Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease Abandoned AU6754196A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP21795095 1995-08-25
JP7-217950 1995-08-25
PCT/JP1996/002343 WO1997008194A1 (en) 1995-08-25 1996-08-22 Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease

Publications (1)

Publication Number Publication Date
AU6754196A true AU6754196A (en) 1997-03-19

Family

ID=16712259

Family Applications (1)

Application Number Title Priority Date Filing Date
AU67541/96A Abandoned AU6754196A (en) 1995-08-25 1996-08-22 Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease

Country Status (2)

Country Link
AU (1) AU6754196A (en)
WO (1) WO1997008194A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
JP2863638B2 (en) * 1996-01-26 1999-03-03 ベーリンガー マンハイム コーポレイション Bismaleimide crosslinking agent
WO2001062972A1 (en) 2000-02-25 2001-08-30 Biofocus Discovery Limited Methods for identifying candidate polynucleotide molecules encoding a protease
AU2007290535A1 (en) * 2006-08-28 2008-03-06 Vertex Pharmaceuticals Incorporated Method for identifying protease inhibitors

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0428000A1 (en) * 1989-11-03 1991-05-22 Abbott Laboratories Fluorogenic substrates for the detection of proteolytic enzyme activity

Also Published As

Publication number Publication date
WO1997008194A1 (en) 1997-03-06

Similar Documents

Publication Publication Date Title
AU2933797A (en) Synthetic inhibitors of hepatitis c virus ns3 protease
AU7063598A (en) Hepatitis c ns3 protease inhibitors
AU2055301A (en) Alpha-ketoamide inhibitors of hepatitis c virus ns3 protease
AP9901512A0 (en) Inhibitors of serine proteases particularly hepatitis c virus NS3 protease
AU6371900A (en) Lactam inhibitors of hepatitis c virus ns3 protease
AU7117496A (en) Peptide inhibitor for viral protease derived from the transframe region of the gag-pol gene
AU2002230763A1 (en) Inhibitors of hepatitis c virus ns3 protease
AU2001245356A1 (en) Inhibitors of hepatitis c virus ns3 protease
AU8180598A (en) Screening methods using an atpase protein from a virus of the (flaviviridae) family
AU7127298A (en) Serine protease inhibitors
AU5920400A (en) Cell-based assay systems for examining hcv ns3 protease activity
GB9825946D0 (en) Pharmaceutical compounds for the inhibition of hepatitis C virus NS3 protease
AU8740398A (en) Amplification of nucleic acids
AU4612596A (en) Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore
AU6463198A (en) Correction of emission contamination and deadtime loss
SI0932617T1 (en) Inhibitors of serine proteases, particularly hepatitis c virus ns3 protease
AU1905399A (en) Hepatitis c virus ns5b truncated protein and methods thereof to identify antiviral compounds
AU5854196A (en) Method for refolding insoluble aggregates of hepatitis c vir us protease
AU3334595A (en) Assay for the detection of proteases
AU4973799A (en) Methods for plasma modification of substrates
AU6754196A (en) Novel fluorescent substrate for assaying activity of hepatitis c virus ns3 serine protease
EP1141255A4 (en) Modified forms of hepatitis c virus ns3 protease
AU4672996A (en) Serine protease inhibitors
AU4397097A (en) Soluble polypeptides with activity of the ns3 serine protease of hepatitis c virus, and process for their preparation and isolation
AU7679491A (en) Hepatitis c virus protease inhibitors