AU2021103023A4 - Sichuan mountain black-bone chicken feed and method for preparing the same - Google Patents
Sichuan mountain black-bone chicken feed and method for preparing the same Download PDFInfo
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- AU2021103023A4 AU2021103023A4 AU2021103023A AU2021103023A AU2021103023A4 AU 2021103023 A4 AU2021103023 A4 AU 2021103023A4 AU 2021103023 A AU2021103023 A AU 2021103023A AU 2021103023 A AU2021103023 A AU 2021103023A AU 2021103023 A4 AU2021103023 A4 AU 2021103023A4
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 28
- 150000001875 compounds Chemical class 0.000 claims abstract description 72
- 102000004190 Enzymes Human genes 0.000 claims abstract description 67
- 108090000790 Enzymes Proteins 0.000 claims abstract description 67
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 26
- 230000000813 microbial effect Effects 0.000 claims abstract description 26
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- 235000012424 soybean oil Nutrition 0.000 claims abstract description 18
- 239000003549 soybean oil Substances 0.000 claims abstract description 18
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 15
- 239000011780 sodium chloride Substances 0.000 claims abstract description 15
- 240000008042 Zea mays Species 0.000 claims abstract description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 12
- 235000005822 corn Nutrition 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims abstract description 12
- 239000004575 stone Substances 0.000 claims abstract description 12
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 11
- 150000001413 amino acids Chemical class 0.000 claims abstract description 7
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 6
- 235000019742 Vitamins premix Nutrition 0.000 claims abstract description 6
- 239000000440 bentonite Substances 0.000 claims abstract description 6
- 229910000278 bentonite Inorganic materials 0.000 claims abstract description 6
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims abstract description 6
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims abstract description 6
- 235000019700 dicalcium phosphate Nutrition 0.000 claims abstract description 6
- 239000002245 particle Substances 0.000 claims abstract description 6
- 239000004455 soybean meal Substances 0.000 claims abstract description 6
- 108010058643 Fungal Proteins Proteins 0.000 claims abstract description 5
- 229940088598 enzyme Drugs 0.000 claims description 65
- 239000000203 mixture Substances 0.000 claims description 36
- 238000002156 mixing Methods 0.000 claims description 25
- 108010059892 Cellulase Proteins 0.000 claims description 24
- 229940106157 cellulase Drugs 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 241000186660 Lactobacillus Species 0.000 claims description 18
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 18
- 229940039696 lactobacillus Drugs 0.000 claims description 18
- 239000002994 raw material Substances 0.000 claims description 13
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 12
- 108090000145 Bacillolysin Proteins 0.000 claims description 12
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 12
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 12
- 102100022624 Glucoamylase Human genes 0.000 claims description 12
- 102000035092 Neutral proteases Human genes 0.000 claims description 12
- 108091005507 Neutral proteases Proteins 0.000 claims description 12
- 108010059820 Polygalacturonase Proteins 0.000 claims description 12
- 102000004142 Trypsin Human genes 0.000 claims description 12
- 108090000631 Trypsin Proteins 0.000 claims description 12
- 108010046377 Whey Proteins Proteins 0.000 claims description 12
- 102000007544 Whey Proteins Human genes 0.000 claims description 12
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 12
- 150000004676 glycans Chemical class 0.000 claims description 12
- 229920001282 polysaccharide Polymers 0.000 claims description 12
- 239000005017 polysaccharide Substances 0.000 claims description 12
- 239000003223 protective agent Substances 0.000 claims description 12
- 235000010413 sodium alginate Nutrition 0.000 claims description 12
- 239000000661 sodium alginate Substances 0.000 claims description 12
- 229940005550 sodium alginate Drugs 0.000 claims description 12
- 239000012588 trypsin Substances 0.000 claims description 12
- 235000021119 whey protein Nutrition 0.000 claims description 12
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims description 10
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims description 10
- KPQJOKRSYYJJEL-VLQRKCJKSA-K [Na+].[Na+].CC1(C)[C@H](CC[C@@]2(C)[C@H]1CC[C@]1(C)[C@@H]2C(=O)C=C2[C@@H]3C[C@](C)(CC[C@]3(C)CC[C@@]12C)C([O-])=O)O[C@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O)C([O-])=O)C([O-])=O Chemical compound [Na+].[Na+].CC1(C)[C@H](CC[C@@]2(C)[C@H]1CC[C@]1(C)[C@@H]2C(=O)C=C2[C@@H]3C[C@](C)(CC[C@]3(C)CC[C@@]12C)C([O-])=O)O[C@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O)C([O-])=O)C([O-])=O KPQJOKRSYYJJEL-VLQRKCJKSA-K 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 229960000367 inositol Drugs 0.000 claims description 10
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 10
- 235000018102 proteins Nutrition 0.000 claims description 10
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 claims description 10
- 229940016667 resveratrol Drugs 0.000 claims description 10
- 235000021283 resveratrol Nutrition 0.000 claims description 10
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 10
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 10
- 235000012141 vanillin Nutrition 0.000 claims description 10
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 10
- 241000186604 Lactobacillus reuteri Species 0.000 claims description 9
- 241000235342 Saccharomycetes Species 0.000 claims description 9
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims description 9
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 9
- 235000011164 potassium chloride Nutrition 0.000 claims description 9
- 239000001103 potassium chloride Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000007605 air drying Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 5
- 230000003179 granulation Effects 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 229920000447 polyanionic polymer Polymers 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000009210 therapy by ultrasound Methods 0.000 claims description 4
- 244000199866 Lactobacillus casei Species 0.000 claims description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- 229960001322 trypsin Drugs 0.000 claims description 2
- 244000228451 Stevia rebaudiana Species 0.000 claims 2
- 235000013330 chicken meat Nutrition 0.000 abstract description 56
- 238000004519 manufacturing process Methods 0.000 abstract description 21
- 235000013601 eggs Nutrition 0.000 abstract description 19
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 229940079593 drug Drugs 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 235000013311 vegetables Nutrition 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 241000544066 Stevia Species 0.000 description 8
- 238000003307 slaughter Methods 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 5
- 230000037213 diet Effects 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- 210000002976 pectoralis muscle Anatomy 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000037396 body weight Effects 0.000 description 3
- 235000021050 feed intake Nutrition 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- KUNICNFETYAKKO-UHFFFAOYSA-N sulfuric acid;pentahydrate Chemical compound O.O.O.O.O.OS(O)(=O)=O KUNICNFETYAKKO-UHFFFAOYSA-N 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Fodder In General (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present application discloses a Sichuan mountain black-bone chicken feed and a
method for preparing the same, belonging to the technical field of feeds. The Sichuan
mountain black-bone chicken feed includes corn, soybean meal, yeast protein, corn
protein powder, soybean oil, stone powder, stone particle, calcium hydrogen
phosphate, vitamin premix, microelement premix, compound microbial agent,
compound enzyme, amino acid premix, table salt and bentonite. The reasonable
proportioning of the compound microbial agent and the compound enzyme in the
formula of the present application improves the absorption efficiency of nutrients,
enhances the immunity of the body, reduces the incidence of diseases, and avoids the
use of drugs at the breeding end, thus ensuring the production of safe, reliable and
excellent-quality black-bone chicken eggs and black-bone chicken meat, meeting the
consumption demand of people for free-range chicken eggs and free-range chicken
meat, and promoting the development of the vegetable basket livelihood project.
Description
Description
Technical Field
The present application belongs to the technical field of feeds, and in particular relates
to a Sichuan mountain black-bone chicken feed and a method for preparing the same.
Background Art
Sichuan mountain black-bone chicken are a kind of free-range chicken for purpose of
both meat and eggs, which are produced by Sichuan local chicken species black-bone
chicken through closed breeding, are mainly produced in the southern and southwest
mountainous areas of Sichuan Province, and are widely distributed in large quantity.
This kind of chicken has the characteristics of wide adaptability, strong disease
resistance and stable genetic performance. The ecological free-range method is
usually adopted for Sichuan mountain black-bone chicken. According to the detection,
the diet only contains 11%-12% of crude protein. If the chicken are fed insufficiently,
it will easily cause nutrition imbalance, which will cause low egg production and poor
meat production performance. At the same time, it is difficult to collect eggs in this
feeding mode. It has been found that ll%-12% of the crude protein in the diet of
laying hens is enough theoretically, but in order to achieve better production results,
the actual amount of the crude protein is required to be no less than 14%.
Moreover, due to the shortage of high-quality protein feed materials in China, the use
of the large amount of unconventional protein raw materials in the Sichuan mountain
black-bone chicken feed has a great negative impact on the breeding efficiency. At the
Description
same time, the activity of the microbial agent and enzyme in the existing feed will
decrease with time, which leads to instable absorption of crude protein in the feed,
thus greatly affecting the production and slaughter performance of the Sichuan
mountain black-bone chicken.
At present, there is a lack of research on the Sichuan mountain black-bone chicken
feed. In order to improve the meat and egg quality of Sichuan mountain black-bone
chicken and promote the large-scale breeding and production of Sichuan local
high-quality chicken breeds, it is necessary to carry out relevant research on the
preparation of the Sichuan mountain black-bone chicken feed, especially the
application of the microbial agent and enzyme in the feed.
Summary
In view of the defects of the prior art, the present application aims at providing a
Sichuan mountain black-bone chicken feed and a method for preparing the same.
In order to achieve the purpose, the present application adopts the following technical
solution:
A Sichuan mountain black-bone chicken feed includes the following components in
parts by weight: 40-80 parts of corn, 20-30 parts of soybean meal, 5-10 parts of yeast
protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone
powder, 2-4 parts of stone particle, 1-2 parts of calcium hydrogen phosphate, 0.1-0.2
part of vitamin premix, 0.1-0.3 part of microelement premix, 2-4 parts of compound
microbial agent, 1-5 parts of compound enzyme, 1-2 parts of amino acid premix,
0.2-0.4 part of table salt and 0.4-0.6 part of bentonite.
Further, the compound microbial agent includes any four of Saccharomycetes,
Description
Lactobacillus, Lactobacillus casei, Lactobacillus crimpus and Lactobacillus reuteri.
Further, the compound microbial agent includes Saccharomycetes, Lactobacillus,
Lactobacillus crimpus and Lactobacillus reuteri.
Further, the compound microbial agent includes the following components in parts by
weight: 4-8 parts of Saccharomycetes, 2-4 parts of Lactobacillus, 2-4 parts of
Lactobacillus crimpus and 1-3 parts of Lactobacillus reuteri.
Further, the compound enzyme includes a compound enzyme I and a compound
enzyme II; the complex enzyme I includes p-glucanase, pectinase, xylanase, cellulase,
glucoamylase, neutral protease and a protective agent; the complex enzyme II
includes trypsin, cellulase, sodium alginate, polyanionic polysaccharide, whey protein
isolate and soybean oil.
Further, the mass ratio of the compound enzyme I to the compound enzyme II is
-15:4-6; the complex enzyme I includes the following components in parts by
weight: 1-3 parts of p-glucanase, 1-3 parts of pectinase, 1-2 parts of xylanase, 1-2
parts of cellulase, 1-3 parts of glucoamylase, 1-3 parts of neutral protease and 2-3
parts of the protective agent; the complex enzyme II includes the following
components in parts by weight: 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of
sodium alginate, 0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein
isolate and 0.5-1 part of soybean oil.
Further, the protective agent includes the following components in parts by weight:
-10 parts of disodium glycyrrhizinate, 4-6 parts of vanillin, 4-6 parts of sodium
chloride, 2-4 parts of Stevia sugar, 2-4 parts of potassium chloride, 1-3 parts of copper
sulfate pentahydrate, 1-2 parts of resveratrol and 1-2 parts of meso-inositol.
Description
A method for preparing the Sichuan mountain black-bone chicken feed includes the
following steps: weighing raw materials according to proportions, uniformly mixing
the raw materials, then adding water, performing uniform stirring, and then
sequentially performing granulation and air drying to obtain the Sichuan mountain
black-bone chicken feed.
Further, a method for preparing the compound enzyme I includes the following steps:
mixing disodium glycyrrhizinate, vanillin, sodium chloride, Stevia sugar, potassium
chloride and copper sulfate pentahydrate, crushing the mixture to 70-mesh to 80-mesh,
placing and treating the mixture for 3-4min in 70-80C steam, then rapidly cooling the
mixture to 20-24C, and standing for 1-2h to obtain a mixture I; mixing resveratrol
and meso-inositol, crushing the mixture to 30-mesh to 36-mesh, then adding 100-200
parts of ethanol solution with a mass fraction of 80-95%, performing ultrasonic
treatment for 50-80min at 30-40C and ultrasonic frequency of 200-300W, then
performing filtration, taking and heating the filtrate to remove ethanol, then
performing freezing for 8-9h at -10° to -20°C, and then performing vacuum drying for
3-5h to obtain a mixtureII; uniformly mixing p-glucanase, pectinase, xylanase,
cellulase, glucoamylase and neutral protease with the mixture I and the mixture II to
obtain the compound enzyme I.
Further, a method for preparing the compound enzyme II includes the following steps:
uniformly mixing trypsin and cellulase, then adding sodium alginate and polyanion
polysaccharide for coating, and finally adding mixed whey protein isolate and
soybean oil for coating to obtain the compound enzyme II.
Further, the vitamin premix, the microelement premix and the amino acid premix are
commercially available premix.
Description
The microbial agent and enzyme are ideal functional raw materials. The present
application investigates the diet prepared by using the microbial agent and enzyme,
studies the influence on the production performance and slaughter performance of the
Sichuan mountain black-bone chicken, and provides experimental basis for giving full
play to the production potential of the Sichuan mountain black-bone chicken and
improving the production performance.
The present application has the following beneficial effects:
By feeding the Sichuan mountain black-bone chicken with the compound feed
containing multiple compound microbial agents and compound enzymes, the present
application reduces the use of unconventional feed resources, improves the protein
digestibility, saves the protein feed raw materials, and improves the breeding
efficiency. Moreover, by feeding the Sichuan mountain black-bone chicken with the
feed in the formula of the present application, the egg production percentage and meat
production percentage can be significantly improved. The reasonable proportioning of
the compound microbial agent and the compound enzyme in the formula of the
present application improves the absorption efficiency of nutrients, enhances the
immunity of the body, reduces the incidence of diseases, and avoids the use of drugs
at the breeding end, thus ensuring the production of safe, reliable and
excellent-quality black-bone chicken eggs and black-bone chicken meat, meeting the
consumption demand of people for free-range chicken eggs and free-range chicken
meat, and promoting the development of the vegetable basket livelihood project.
Description of the Embodiments
In order to further describe the technical effect of the present application, the present
application will be specifically described below through examples.
Description
Example 1
A Sichuan mountain black-bone chicken feed included the following components in
parts by weight: 40 parts of corn, 20 parts of soybean meal, 5 parts of yeast protein, 5
parts of corn protein powder, 1 part of soybean oil, 3 parts of stone powder, 2 parts of
stone particle, 1 part of calcium hydrogen phosphate, 0.1 part of vitamin premix, 0.1
part of microelement premix, 2 parts of compound microbial agent, 1 part of
compound enzyme, 1part of amino acid premix, 0.2 part of table salt and 0.4 part of
bentonite.
The compound microbial agent included the following components in parts by weight:
4 parts of Saccharomycetes, 2 parts of Lactobacillus, 2 parts of Lactobacillus crimpus
and 1 part of Lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II was 10:4; the
complex enzyme I included the following components in parts by weight: 1 part of
j-glucanase, 1part of pectinase, 1 part of xylanase, 1 part of cellulase, 1 part of
glucoamylase, 1 part of neutral protease and 2 parts of the protective agent; the
complex enzyme II included the following components in parts by weight: 4 parts of
trypsin, 2 parts of cellulase, 1 part of sodium alginate, 0.5 part of polyanionic
polysaccharide, 0.5 part of whey protein isolate and 0.5 part of soybean oil.
The protective agent included the following components in parts by weight: 5 parts of
disodium glycyrrhizinate, 4 parts of vanillin, 4 parts of sodium chloride, 2 parts of
Stevia sugar, 2 parts of potassium chloride, 1 part of copper sulfate pentahydrate, 1
part of resveratrol and 1 part of meso-inositol.
Description
A method for preparing the compound enzyme I included the following steps: mixing
disodium glycyrrhizinate, vanillin, sodium chloride, Stevia sugar, potassium chloride
and copper sulfate pentahydrate, crushing the mixture to 70-mesh, placing and
treating the mixture for 3min in 70C steam, then rapidly cooling the mixture to 20C,
and standing for 1h to obtain a mixtureI; mixing resveratrol and meso-inositol,
crushing the mixture to 30-mesh, then adding 100 parts of ethanol solution with a
mass fraction of 80%, performing ultrasonic treatment for 50min at 30C and
ultrasonic frequency of 200W, then performing filtration, taking and heating the
filtrate to remove ethanol, then performing freezing for 8h at -10° to -20°C, and then
performing vacuum drying for 3h to obtain a mixture II; uniformly mixing
j-glucanase, pectinase, xylanase, cellulase, glucoamylase and neutral protease with
the mixture I and the mixture II to obtain the compound enzyme I.
A method for preparing the compound enzyme II included the following steps:
uniformly mixing trypsin and cellulase, then adding sodium alginate and polyanion
polysaccharide for coating, and finally adding mixed whey protein isolate and
soybean oil for coating to obtain the compound enzyme II.
A method for preparing the Sichuan mountain black-bone chicken feed included the
following steps: weighing raw materials according to proportions, uniformly mixing
the raw materials, then adding water, performing uniform stirring, and then
sequentially performing granulation and air drying to obtain the Sichuan mountain
black-bone chicken feed.
Example 2
A Sichuan mountain black-bone chicken feed included the following components in
parts by weight: 80 parts of corn, 30 parts of soybean meal, 10 parts of yeast protein,
Description
parts of corn protein powder, 3 parts of soybean oil, 5 parts of stone powder, 4
parts of stone particle, 2 parts of calcium hydrogen phosphate, 0.2 part of vitamin
premix, 0.3 part of microelement premix, 4 parts of compound microbial agent, 5
parts of compound enzyme, 2 parts of amino acid premix, 0.4 part of table salt and 0.6
part of bentonite.
The compound microbial agent included the following components in parts by weight:
8 parts of Saccharomycetes, 4 parts of Lactobacillus, 4 parts of Lactobacillus crimpus
and 3 parts of Lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II was 15:6; the
complex enzyme I included the following components in parts by weight: 3 parts of
j-glucanase, 3 parts of pectinase, 2 parts of xylanase, 2 parts of cellulase, 3 parts of
glucoamylase, 3 parts of neutral protease and 3 parts of the protective agent; the
complex enzyme II included the following components in parts by weight: 6 parts of
trypsin, 3 parts of cellulase, 2 parts of sodium alginate, 1 part of polyanionic
polysaccharide, 1 part of whey protein isolate and 1 part of soybean oil.
The protective agent included the following components in parts by weight: 10 parts
of disodium glycyrrhizinate, 6 parts of vanillin, 6 parts of sodium chloride, 4 parts of
Stevia sugar, 4 parts of potassium chloride, 3 parts of copper sulfate pentahydrate, 2
parts of resveratrol and 2 parts of meso-inositol.
A method for preparing the compound enzyme I included the following steps: mixing
disodium glycyrrhizinate, vanillin, sodium chloride, Stevia sugar, potassium chloride
and copper sulfate pentahydrate, crushing the mixture to 80-mesh, placing and
treating the mixture for 4min in 80°C steam, then rapidly cooling the mixture to 24°C,
and standing for 2h to obtain a mixtureI; mixing resveratrol and meso-inositol,
Description
crushing the mixture to 36-mesh, then adding 200 parts of ethanol solution with a
mass fraction of 95%, performing ultrasonic treatment for 80min at 40C and
ultrasonic frequency of 300W, then performing filtration, taking and heating the
filtrate to remove ethanol, then performing freezing for 9h at -10° to -20°C, and then
performing vacuum drying for 5h to obtain a mixture II; uniformly mixing
j-glucanase, pectinase, xylanase, cellulase, glucoamylase and neutral protease with
the mixture I and the mixture II to obtain the compound enzyme I.
A method for preparing the compound enzyme II included the following steps:
uniformly mixing trypsin and cellulase, then adding sodium alginate and polyanion
polysaccharide for coating, and finally adding mixed whey protein isolate and
soybean oil for coating to obtain the compound enzyme II.
A method for preparing the Sichuan mountain black-bone chicken feed included the
following steps: weighing raw materials according to proportions, uniformly mixing
the raw materials, then adding water, performing uniform stirring, and then
sequentially performing granulation and air drying to obtain the Sichuan mountain
black-bone chicken feed.
Example 3
A Sichuan mountain black-bone chicken feed included the following components in
parts by weight: 60 parts of corn, 25 parts of soybean meal, 7 parts of yeast protein, 7
parts of corn protein powder, 2 parts of soybean oil, 2 parts of stone powder, 3 parts of
stone particle, 1 part of calcium hydrogen phosphate, 0.15 part of vitamin premix, 0.2
part of microelement premix, 3 parts of compound microbial agent, 3 parts of
compound enzyme, 2 parts of amino acid premix, 0.3 part of table salt and 0.5 part of
bentonite.
Description
The compound microbial agent included the following components in parts by weight:
6 parts of Saccharomycetes, 3 parts of Lactobacillus, 3 parts of Lactobacillus crimpus
and 2 parts of Lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II was 12:5; the
complex enzyme I included the following components in parts by weight: 2 parts of
j-glucanase, 2 parts of pectinase, 1part of xylanase, 2 parts of cellulase, 2 parts of
glucoamylase, 2 parts of neutral protease and 2 parts of the protective agent; the
complex enzyme II included the following components in parts by weight: 5 parts of
trypsin, 3 parts of cellulase, 1.5 parts of sodium alginate, 0.7 part of polyanionic
polysaccharide, 0.7 part of whey protein isolate and 0.7 part of soybean oil.
The protective agent included the following components in parts by weight: 7 parts of
disodium glycyrrhizinate, 5 parts of vanillin, 5 parts of sodium chloride, 3 parts of
Stevia sugar, 3 parts of potassium chloride, 2 parts of copper sulfate pentahydrate, 2
parts of resveratrol and 1 part of meso-inositol.
A method for preparing the compound enzyme I included the following steps: mixing
disodium glycyrrhizinate, vanillin, sodium chloride, Stevia sugar, potassium chloride
and copper sulfate pentahydrate, crushing the mixture to 75-mesh, placing and
treating the mixture for 3min in 75°C steam, then rapidly cooling the mixture to 22°C,
and standing for lh to obtain a mixtureI; mixing resveratrol and meso-inositol,
crushing the mixture to 33-mesh, then adding 150 parts of ethanol solution with a
mass fraction of 90%, performing ultrasonic treatment for 65min at 35°C and
ultrasonic frequency of 250W, then performing filtration, taking and heating the
filtrate to remove ethanol, then performing freezing for 8h at -10° to -20°C, and then
performing vacuum drying for 5h to obtain a mixture II; uniformly mixing
Description
j-glucanase, pectinase, xylanase, cellulase, glucoamylase and neutral protease with
the mixture I and the mixture II to obtain the compound enzyme I.
A method for preparing the compound enzyme II included the following steps:
uniformly mixing trypsin and cellulase, then adding sodium alginate and polyanion
polysaccharide for coating, and finally adding mixed whey protein isolate and
soybean oil for coating to obtain the compound enzyme II.
A method for preparing the Sichuan mountain black-bone chicken feed included the
following steps: weighing raw materials according to proportions, uniformly mixing
the raw materials, then adding water, performing uniform stirring, and then
sequentially performing granulation and air drying to obtain the Sichuan mountain
black-bone chicken feed.
Comparative example 1
In this comparative example, no compound microbial agent was added, and other
components and preparation methods were the same as that in example 1.
Comparative example 2
In this comparative example, no compound enzyme was added, and other components
and preparation methods were the same as that in example 1.
Comparative example 3
In this comparative example, neither compound microbial agent nor compound
enzyme was added, and other components and preparation methods were the same as
that in example 1.
Description
1 Materials and methods
1.1 Test design
480 healthy 23-week-old Sichuan mountain black-bone chicken with similar body
weight were selected and fed with the feeds prepared in examples 1-3 and
comparative examples 1-3 to carry out a test. The test was divided into 6 treatments,
each treatment included 4 replicates and each replicate included 20 chicken. The
pre-test period was 14 days and the formal test period was 120 days.
1.2 Diets for test and feeding management
The diets for test were prepared according to the China "Chicken Feeding Standard"
(NY/T33-2004) and the China Feed Composition and Nutritional Value Table (2020),
in combination with the Technical Guide for Breeding of Sichuan Mountain
Black-Bone Chicken. In order to meet the welfare and healthy breeding of chicken,
the test chicken were bred on roosts outside the house instead of free range. In
addition, the laying boxes were mounted to facilitate the collection of eggs. During
the test period, natural light illumination, natural ventilation, free feeding and water
drinking were adopted. The chicken were immunized according to a routine
immunization procedure.
1.3 Determination indexes and methods
1.3.1 Production performance
During the test period, the daily egg production, egg weight and feed intake were
recorded in unit of replicate, and the feed-to-egg ratio was calculated according to the
daily egg production and feed intake.
Description
1.3.2 Slaughter performance
After the test, four chicken with body weight close to average body weight were
selected from each replicate, and the selected chicken were executed by means of
neck bleeding. The hairs were removed in 70° C hot water, and the dressing
percentage, eviscerating percentage, semi-eviscerating percentage, chest muscle
percentage and leg muscle percentage were measured.
Dressing percentage (%)= (dressed weight/body weight before slaughter)*100%
Eviscerating percentage (%)= eviscerated weight/weight before slaughter)*100%
Semi-eviscerating percentage (%) = eviscerated weight with giblet/weight before
slaughter)*100%
Chest muscle percentage (%)= (chest muscle weight/eviscerated weight)*100%
Leg muscle percentage (%)= (leg muscle weight/eviscerated weight)*100%
Description
2 Results and analysis
Table 1 Influences of different feeds on production performance of Sichuan mountain
black-bone chicken
Egg
Average egg production Daily egg Daily feed Feed-to-egg Item weight (g) percentage production (g) intake (g) ratio
Example 1 57.04±1.12a 67.14±1.75a 38.30 ±1.71a 108.67i4.69a 2 .8 4 +0.09b Example 2 57.53±1.16a 69.21±l.62a 39.82±l.96a 110.67±5.44a 2 .7 8 +0.05b Example 3 57.16±1.24a 68.46±l.65a 39.13±l.65a 110.41+5.57a 2.82+0.08b Comparative 5 3 .2 1+1.11b 6 2 .3 3 +1. 4 5b 3 3 . 1 7 +1. 4 5b 9 8 .7 6 + 3 .4 3b 2.98±0.05a example 1 Comparative 5 3 .15+1. 0 2b 6 1 .5 3 +1. 2 7b 32.70+1. 2 5b 9 7 .53+ 3 .12b 2.98i0.10a example 2 Comparative 51.27+1.04 60.17+1. 0 7b 30.85+1.35b 9 5.7 6 + 4 .7 8b 3.10i0.06a example 3
Notes: data in the same line with the same letter shoulder note have no significant
difference (P > 0.05); data in the same line with different low-case letter shoulder
notes have significant difference (P < 0.05), similarly hereinafter.
Description
Table 2 Influences of different feeds on slaughter performance of Sichuan mountain
black-bone chicken (unit: %)
Chest Semi-eviscerating muscle Leg muscle Item Dressing Eviscerating percentage percentage percentage percentage percentage
92.56± Example 1 1.43a 66.58±0.62a 80.45±1.01a 18.67±1.13a 21.32±0.81a
93.42+ Example 2 1.22a 68.42±0.53a 81.93±0.91a 20.95±0.88a 23.77±0.67a
92.68± Example 3 4a 67.58±0.57a 80.86±0.68a 19.45±0.92a 21.57±0.54a
Comparativ 89.54±i 16.49± i exmplev1 9.54b 6 3 .3 2 +0. 4 5b 77.51+0.54' 18.67+0.43b e example 1 1.01b 0.67 b
Comparative 87.32±i 15.68±i empe2 87.32b 6 2 .6 1+0. 4 7b 76.89+0.46 17.86+0.54b e example 2 1.06 b 0.98 b
-exmple3tv 87.04b 62.58+0.31b 76.56+0.55b 1545 16.77+0.67b e example 3 1.02 b 0.67 b
From Table 1 and Table 2, it can be seen that, compared with comparative examples
1-3, the production performance and slaughter performance of Sichuan mountain
black-bone chicken in examples 1-3 are better. It is proved that the Sichuan mountain
black-bone chicken feed containing the compound microbial agent and the compound
enzyme and the method for preparing the same in the solution of the present
application can significantly improve the average egg weight, egg production
percentage, daily egg production, daily feed intake and feed-to-egg ratio of the
Sichuan mountain black-bone chicken. It is proved that the feed containing the
compound microbial agent and the compound enzyme and the method for preparing
the same in the solution of the present application can significantly improve the
dressing percentage, eviscerating percentage, semi-eviscerating percentage, chest muscle
percentageandleg muscle percentage of the Sichuan mountain black-bone chicken.
Description
Finally, it should be noted that the above examples are only used for describing the technical
solution of the present application instead of limiting it. Although the technical solution of the
present application has been described in detail with reference to the examples, those skilled in the
art should understand that modifications or equivalent replacements may be made to the technical
solution of the present application without departing from the essence and scope of the present
application, which, however, should also be included in the scope of protection of the present
application.
Claims (5)
1. A Sichuan mountain black-bone chicken feed, wherein the Sichuan mountain
black-bone chicken feed comprises the following components in parts by weight:
-80 parts of corn, 20-30 parts of soybean meal, 5-10 parts of yeast protein, 5-10
parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4
parts of stone particle, 1-2 parts of calcium hydrogen phosphate, 0.1-0.2 part of
vitamin premix, 0.1-0.3 part of microelement premix, 2-4 parts of compound
microbial agent, 1-5 parts of compound enzyme, 1-2 parts of amino acid premix,
0.2-0.4 part of table salt and 0.4-0.6 part of bentonite.
2. The Sichuan mountain black-bone chicken feed according to claim 1, wherein
the compound microbial agent comprises any four of Saccharomycetes, Lactobacillus,
Lactobacillus casei, Lactobacillus crimpus and Lactobacillus reuteri;
wherein the compound microbial agent comprises Saccharomycetes, Lactobacillus,
Lactobacillus crimpus and Lactobacillus reuteri;
wherein the compound microbial agent comprises the following components in parts
by weight: 4-8 parts of Saccharomycetes, 2-4 parts of Lactobacillus, 2-4 parts of
Lactobacillus crimpus and 1-3 parts of Lactobacillus reuteri.
2. The Sichuan mountain black-bone chicken feed according to any one of claims 1,
wherein the compound enzyme comprises a compound enzyme I and a compound
enzyme II; the complex enzyme I comprises p-glucanase, pectinase, xylanase,
cellulase, glucoamylase, neutral protease and a protective agent; the complex enzyme
II comprises trypsin, cellulase, sodium alginate, polyanionic polysaccharide, whey
protein isolate and soybean oil.
3. The Sichuan mountain black-bone chicken feed according to claim 2, wherein
the mass ratio of the compound enzyme I to the compound enzyme II is 10-15:4-6;
the complex enzyme I comprises the following components in parts by weight: 1-3
Claims
parts of p-glucanase, 1-3 parts of pectinase, 1-2 parts of xylanase, 1-2 parts of
cellulase, 1-3 parts of glucoamylase, 1-3 parts of neutral protease and 2-3 parts of the
protective agent; the complex enzyme II comprises the following components in parts
by weight: 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of sodium alginate,
0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein isolate and 0.5-1
part of soybean oil;
wherein the protective agent comprises the following components in parts by weight:
-10 parts of disodium glycyrrhizinate, 4-6 parts of vanillin, 4-6 parts of sodium
chloride, 2-4 parts of Stevia sugar, 2-4 parts of potassium chloride, 1-3 parts of copper
sulfate pentahydrate, 1-2 parts of resveratrol and 1-2 parts of meso-inositol.
4. A method for preparing the Sichuan mountain black-bone chicken feed
according to claim 3, wherein the method comprises the following steps: weighing
raw materials according to proportions, uniformly mixing the raw materials, then
adding water, performing uniform stirring, and then sequentially performing
granulation and air drying to obtain the Sichuan mountain black-bone chicken feed;
wherein a method for preparing the compound enzyme I comprises the following
steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, Stevia sugar,
potassium chloride and copper sulfate pentahydrate, crushing the mixture to 70-mesh
to 80-mesh, placing and treating the mixture for 3-4min in 70-80C steam, then
rapidly cooling the mixture to 20-24C, and standing for 1-2h to obtain a mixture I;
mixing resveratrol and meso-inositol, crushing the mixture to 30-mesh to 36-mesh,
then adding 100-200 parts of ethanol solution with a mass fraction of 80- 9 5 %,
performing ultrasonic treatment for 50-80min at 30-40C and ultrasonic frequency of
200-300W, then performing filtration, taking and heating the filtrate to remove
ethanol, then performing freezing for 8-9h at -10° to -20C, and then performing
Claims
vacuum drying for 3-5h to obtain a mixtureII; uniformly mixing p-glucanase,
pectinase, xylanase, cellulase, glucoamylase and neutral protease with the mixture I
and the mixture II to obtain the compound enzyme I.
5. The method according to claim 4, wherein a method for preparing the compound
enzyme II comprises the following steps: uniformly mixing trypsin and cellulase, then
adding sodium alginate and polyanion polysaccharide for coating, and finally adding
mixed whey protein isolate and soybean oil for coating to obtain the compound
enzyme II.
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| CN102940135B (en) * | 2012-09-25 | 2013-09-18 | 东莞市双胞胎饲料有限公司 | Efficient pig feed composite enzyme preparation added with enzyme protective agent |
| CN103892134B (en) * | 2014-04-15 | 2015-09-16 | 湖南农业大学 | A kind of egg feedstuff complex enzyme formulation and production method thereof and application |
| CN104171538B (en) * | 2014-07-07 | 2016-04-13 | 辽宁禾丰牧业股份有限公司 | A kind of grain-saving type laying hen in egg laying peak period daily ration and preparation method thereof |
| CN105524905A (en) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | Acid protease containing compound enzyme of soy sauce and preparation method of acid protease containing compound enzyme of soy sauce |
| CN104957427A (en) * | 2015-07-14 | 2015-10-07 | 四川圣迪乐村生态食品股份有限公司 | Layer feed for keeping layer high yield and preventing fatty liver |
| CN105192379A (en) * | 2015-10-08 | 2015-12-30 | 南涧县兴农发展有限公司 | Ecological-type high-quality concentrated feed for Wuliang mountain silky fowl |
| CN106666204A (en) * | 2017-01-05 | 2017-05-17 | 辽宁禾丰牧业股份有限公司 | Feed used for 72-100-week-old laying hens and capable of improving immune function and preparation method of feed |
| CN109043210A (en) * | 2018-07-23 | 2018-12-21 | 广西万寿谷投资集团股份有限公司 | A kind of free-ranging chicken chicken feed and preparation method thereof |
| CN109329583A (en) * | 2018-12-12 | 2019-02-15 | 海城市盛利饲料有限公司 | A kind of broiler fodder |
| CN110558448A (en) * | 2019-10-21 | 2019-12-13 | 宁夏晓鸣农牧股份有限公司 | Feed for chickens in egg producing period and preparation method thereof |
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