AU2020258915B2 - Analyte collecting device, and analyte collecting method and analyte inspection system using same - Google Patents
Analyte collecting device, and analyte collecting method and analyte inspection system using same Download PDFInfo
- Publication number
- AU2020258915B2 AU2020258915B2 AU2020258915A AU2020258915A AU2020258915B2 AU 2020258915 B2 AU2020258915 B2 AU 2020258915B2 AU 2020258915 A AU2020258915 A AU 2020258915A AU 2020258915 A AU2020258915 A AU 2020258915A AU 2020258915 B2 AU2020258915 B2 AU 2020258915B2
- Authority
- AU
- Australia
- Prior art keywords
- section
- analyte
- internal space
- case
- aggregating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012491 analyte Substances 0.000 title claims abstract description 225
- 238000000034 method Methods 0.000 title claims abstract description 52
- 238000007689 inspection Methods 0.000 title abstract description 6
- 238000005192 partition Methods 0.000 claims abstract description 80
- 230000004931 aggregating effect Effects 0.000 claims description 112
- 239000000126 substance Substances 0.000 claims description 106
- 239000000243 solution Substances 0.000 claims description 81
- 238000002347 injection Methods 0.000 claims description 63
- 239000007924 injection Substances 0.000 claims description 63
- 102000039446 nucleic acids Human genes 0.000 claims description 35
- 108020004707 nucleic acids Proteins 0.000 claims description 35
- 150000007523 nucleic acids Chemical class 0.000 claims description 35
- 238000012545 processing Methods 0.000 claims description 31
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 30
- 239000012620 biological material Substances 0.000 claims description 27
- 238000007789 sealing Methods 0.000 claims description 21
- 230000009089 cytolysis Effects 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 239000012149 elution buffer Substances 0.000 claims description 6
- -1 vesicle Chemical class 0.000 claims description 6
- 150000001720 carbohydrates Chemical class 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 5
- 239000012148 binding buffer Substances 0.000 claims description 4
- 238000007667 floating Methods 0.000 claims description 4
- 239000012139 lysis buffer Substances 0.000 claims description 4
- 239000011534 wash buffer Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 description 18
- 238000003752 polymerase chain reaction Methods 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000027455 binding Effects 0.000 description 7
- 238000009739 binding Methods 0.000 description 6
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 208000037797 influenza A Diseases 0.000 description 6
- 238000007670 refining Methods 0.000 description 6
- 239000006249 magnetic particle Substances 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 239000005060 rubber Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 210000001808 exosome Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910000077 silane Inorganic materials 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 239000013013 elastic material Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 238000010146 3D printing Methods 0.000 description 1
- 239000004135 Bone phosphate Substances 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000000071 blow moulding Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000748 compression moulding Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- ZFTFAPZRGNKQPU-UHFFFAOYSA-N dicarbonic acid Chemical compound OC(=O)OC(O)=O ZFTFAPZRGNKQPU-UHFFFAOYSA-N 0.000 description 1
- 125000004177 diethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000002122 magnetic nanoparticle Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 238000010107 reaction injection moulding Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000001175 rotational moulding Methods 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000004528 spin coating Methods 0.000 description 1
- 238000003856 thermoforming Methods 0.000 description 1
- 238000001721 transfer moulding Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00306—Housings, cabinets, control panels (details)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00564—Handling or washing solid phase elements, e.g. beads
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The present invention relates to an analyte collecting device, and an analyte collecting method and an analyte inspection system using same. Specifically, an embodiment of the present invention may provide an analyte collecting device, and an analyte collecting method and an analyte inspection system using same, the analyte collecting device comprising: a case including an opening and an inner space; and a piston which includes at least one partition wall for dividing the inner space into multiple inner spaces and is provided to be inserted in the inner space through the opening of the case and be then reciprocally movable therein.
Description
Field of the disclosure
The present disclosure relates to an analyte collecting device, and an analyte collecting
method and an analyte inspection system using the same.
Related Art
In general, samples obtained from a human body or the body of animal are refined and
then undergo a predetermined examination in a laboratory in some cases. In this case,
generally, preprocessing and processing such as refinement are performed on a sample, and the
refined sample may be finally collected as analyte and a predetermined test may be performed.
As an example of an analyte collecting device and method, and an analyte inspection system,
a device and method of refining nucleic acid and a system for examining the refined nucleic
acid may be exemplified.
Refinement of nucleic acid, which is a necessary technique that is widely used in the
fields of genetic engineering and molecular biology, is a very important technique in terms of
study, medical treatment, and industry as a preprocessing stage for techniques such as Southern
blot, Northern blot, and polymerase chain reaction (PCR). Such refinement of nucleic acid is
conventionally achieved through chemical and physical method that use ultrasonic waves, heat,
proteinase, alcohols, a special reagent, etc. and researchers perform a nucleic acid refinement
process using a pipette. However, recently, methods of more conveniently refining nucleic
acid using magnetic particles have been introduced in a wide filed. However, these methods
should be performed in laboratories, require a large amount of time and manpower, and have
limitation to be generally used.
A process of refining nucleic acid includes stages such as dissolving (lysis) of biomaterials such as a cell, nucleic acid-magnetic particle binding, washing, and elution, and requires reagents and treatments suitable for each of the stages. An analyte that has undergone such a refinement process can be collected by a predetermined amount and predetermined necessary tests can be performed on the analyte. That is, refined nucleic acid is moved into a transparent amplification and detection tube and is amplified by real-time PCR or similar techniques, whether there is pathogenic nucleic acid is optically detected using fluorescent labeling, and accordingly, it is possible to diagnose corresponding diseases. An analyte collection device for refining and collecting a sample as an analyte in a predetermined quantity should require minimum manpower for the refinement process, should be filled with a predetermined solution for refinement, and should be small to secure mobility, in order to reduce power of a hospital and perform point-of-care testing (POCT). Further, disposability should be secured to prevent contamination by biomaterials, so the device needs to be implemented at a low cost. However, there are little study about an analyte collection device, a method using the analyte collection device, and a system for examining an analyte that completely satisfies those conditions. (Prior Art Document) US 2015-0232916 Al (2015.08.20) It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to at least provide a useful alternative thereto.
SUMMARY Embodiments of the present disclosure provide an analyte collecting device that can be achieved at a low cost, can be achieved in a small size, and can efficiently process a sample through an automated process in order to refine and pre-process a sample using magnetic particles, and an analyte collecting method and a system for examining an analyte using the analyte collecting device. In a first aspect, the present invention provides an analyte collecting device comprising: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space; wherein the case further includes: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate an outside of the case so that a sample can be injected, and wherein the specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section is filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section isfilled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case. In a second aspect, the present invention provides a system for examining an analyte, the system comprising: an analyte collecting device including a case including an opening and an internal space, and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space; and a holder separably holding the analyte collecting device, wherein the case further includes: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate an outside of the case so that a sample can be injected, and wherein the specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes afirst section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section is filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section is filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case. In a third aspect, the present invention provides a method of collecting analyte using an analyte collecting device including: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space of the case to reciprocate in the internal space, the method comprising: putting a sample in the internal space; and collecting the sample as an analyte by sequentially performing a predetermined processing including a plurality of stages while the piston is moved in the internal space; wherein the case further includes: a specimen injection port
3a
including an injection hole formed at the case to allow the internal space to communicate an outside of the case sothatasample canbe injected, andwhereinthe specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section isfilled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section is filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case. Also disclosed herein is an analyte collecting device including: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space. The case may include: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with an outside, a sample put in the internal space being discharged through the exhaust port to the outside of the case; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space. The internal space may be filled with a solution containing a magnetic substance, and the sample input in the internal space may be discharged through the exhaust port as an analyte after undergoing predetermined processing by the solution in the internal space. The one or more partition walls may include four partition walls, the plurality of internal spaces may include a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section may be filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to the magnetic substance, the second section may be filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section may be filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section may be formed adjacent to the third section and in contact with an inner end of the case. the solution filled in the first section may include at least one of a lysis/binding buffer and isopropyl alcohol (2-propanol), the solution filled in the second section may include a washing buffer, and the solution filled in the third section may include an elution buffer. The piston may include a center column. The one or more partition walls may include a plurality of partition walls spaced apart from each other and radially extend from the circumferential surface of the center column, and the internal space may be divided into a plurality of sections by the partition walls and at least some of the divided plurality of sections may be filled with different solutions. The piston may further include: a flange attached to at least one of two surfaces of each of the one or more partition walls provided perpendicular to an insertion direction of the piston, the flange having a circumferential surface provided closer to an inner wall surrounding the internal surface of the case than the circumferential surface of each of the one or more partition walls; and a sealing member provided to surround the circumferential surface of each of the one or more partition walls and being in contact with the inner wall of the case. The case may further include: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate the outside of the case so that a sample can be injected. The specimen injection port may further include a cap sealing the injection hole by selectively covering the injection hole. An aggregating groove may be recessed from an inner wall forming the internal space of the case, the internal space may be filled with a solution containing a magnetic substance, and the magnetic substance may be aggregated in the aggregating groove when a magnetic force is applied toward the aggregating groove from the outside. The analyte that is collected by the analyte collecting device may include at least one of nucleic acid, protein, vesicle, lipid, a carbohydrate, a cell, and a substance separated therefrom.
Also disclosed herein is a system for examining an analyte, the system including: an analyte collecting device including a case including an opening and an internal space, and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space; and a holder separably holding the analyte collecting device. The system may further include: a plunger for translating the piston in the internal space by pushing or pulling a head of the piston; and a controller. The plunger may be controlled by the controller. The internal space may be filled with a solution containing a magnetic substance, predetermined processing may be performed on a sample put in the case by the solution filled in the internal space, and the predetermined processing may include a plurality of stages which are sequentially performed as the controller controls operation of the plunger. The case may include: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with an outside, a sample, which is put in the internal space and undergoes the predetermined processing, being discharged from the case as an analyte; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space. The controller may control the plunger to push the piston toward the blowback portion such that the analyte is discharged through the exhaust port by a blowback phenomenon. The case may have an aggregating groove recessed from an inner wall forming the internal space, and the system may further include: an aggregating device configured to selectively apply a magnetic force toward the aggregating groove so that the magnetic substance is aggregated in the aggregating groove, the aggregating device being controlled by the controller. The system may further include: a de-aggregating device configured to selectively apply a magnetic force to the internal space and being controlled by the controller. The controller may control the de-aggregating device with the magnetic substance aggregated in the aggregating groove so that a magnetic force is applied to the internal space and the magnetic substance aggregated in the aggregating groove is separated. The analyte that is collected by the analyte collecting device may include at least one of nucleic acid, protein, vesicle, lipid, a carbohydrate, a cell, and a substance separated therefrom.
Also disclosed herein is a method of collecting analyte using an analyte collecting device including: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space of the case to reciprocate in the internal space, the method including: putting a sample in the internal space; and collecting the sample as an analyte by sequentially performing a predetermined processing including a plurality of stages while the piston is moved in the internal space. The case may include: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with an outside, the sample accommodated in the internal space being discharged through the exhaust port to the outside of the case; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space. The method may further include discharging the analyte in which the piston moving in the internal space pushes a section contained an analyte undergoing the predetermined processing toward the blowback portion formed at an end portion of the case, and the analyte pressed through the blowback portion is discharged through an exhaust port formed at the case. An aggregating groove may be recessed from an inner wall forming the internal space of the case, and a magnetic substance is contained in a solution filled in the internal space. The method may further include aggregating the magnetic substance in the aggregating groove by applying a magnetic force to the aggregating groove. The one or more partition walls may include four partition walls, the plurality of internal spaces include a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section of the four sections may be formed closest to an opening of the case, the second section may be formed adjacent to the first section with one of the partition walls therebetween, the third section may be formed adjacent to the second section with one of the partition walls therebetween, and the fourth section may be formed adjacent to the third section with one of the partition walls therebetween to be in contact with an inner end of the case, the sample may be put in the first section, and the sequentially performing the predetermined processing may further include: bonding at least a portion of an analyte in a biomaterial contained in the sample to the magnetic substance by dissolving the biomaterial by the solution filled in the first section, disposing the second section above the aggregating groove by moving the piston backward after the magnetic substance bound to at least a portion of the analyte is aggregated in the aggregating groove by applying a magnetic force to the aggregating groove, floating the magnetic substance in the second section in which the magnetic force applied to the aggregating groove is removed, and the magnetic substance aggregated in the aggregating groove is separated and floats into the second section by applying a magnetic force to the internal space; washing at least a portion of the analyte bound to the magnetic substance by a solution filled in the second section; disposing the third section above the aggregating groove by moving the piston backward after the magnetic substance bound to at least a portion of the analyte is aggregated in the aggregating groove by applying a magnetic force to the aggregating groove; floating the magnetic substance in the third section in which the magnetic force applied to the aggregating groove is removed, and the magnetic substance aggregated in the aggregating groove is separated and floats into the third section by applying a magnetic force to the internal space; eluting at least a portion of the analyte bound to the magnetic substance from the magnetic substance by a solution filled in the third section; and aggregating the magnetic substance with at least a portion of the analyte eluted in the aggregating groove by applying a magnetic force to the aggregating groove.
The solution filled in the first section may include at least one of a lysis/binding buffer and isopropyl alcohol (2-propanol), the solution filled in the second section includes a washing buffer, and the solution filled in the third section includes an elution buffer.
The analyte that is collected by the analyte collecting device may include at least one
of nucleic acid, protein, vesicle, lipid, a carbohydrate, a cell, and a substance separated
therefrom.
According to embodiments of the present disclosure, since processing such as
refinement, amplification, detection can be automatically performed without intervention of a
user after a sample is put in the device, there is an effect that usability is high, secondary
infection of a user or a third party can be prevented when the sample is processed.
Further, since the analyte that finishes being refined can be automatically conveyed, a
user does not intervene in movement of a solution for the next reaction, so there is an effect
that sample-to-answer can be implemented and carryover contamination can be prevented.
Further, there is an effect that a yield ratio an analyte is prevented from changing
depending on the skill of users and it is possible to achieve a constant yield ratio of an analyte
every time.
Further, there is an effect that the structure of the device is simplified, the components
can be minimized, the cost is reduced by downsizing, and spatial usability can be increased.
Further, since it is possible to add a plurality of refining stages in one device, there is
an effect that multiple specimens can be processed and detected.
FIG. 1 is a perspective view showing an analyte collecting device according to an
embodiment of the present disclosure.
FIG. 2 is a cross-sectional view taken along line II-II of FIG. 1.
FIG. 3 is a conceptual diagram showing a system for examining an analyte including
the analyte collecting device of FIG. 1.
FIGs. 4A to 4J are flowcharts showing a process of refining and collecting an analyte
using the analyte collecting device of FIG. 1.
FIG. 5 is a vertical cross-sectional view showing an analyte collecting device
according to another embodiment of the present disclosure.
FIG. 6 is a diagram showing a system for examining an analyte according to another
embodiment of the present disclosure.
FIG. 7 is a diagram showing a system for examining an analyte according to another
embodiment of the present disclosure.
FIGS. 8 and 9 are charts showing an examination result of performing PCR on an
analyte collected using the analyte collecting device of FIG. 1.
Hereinafter, specific embodiments for implementing a spirit of the present disclosure
will be described in detail with reference to the drawings.
In describing the present disclosure, detailed descriptions of known configurations or
functions may be omitted to clarify the present disclosure.
When an element is referred to as being 'connected' to, or 'contacted' with another
element, it should be understood that the element may be directly connected to, or contacted
with another element, but that other elements may exist in the middle.
The terms used in the present disclosure are only used for describing specific
embodiments, and are not intended to limit the present disclosure. Singular expressions
include plural expressions unless the context clearly indicates otherwise.
Hereafter, an analyte collecting device and a system for examining an analyte
according to an embodiment of the present disclosure are described with reference to the
drawings.
Referring FIGS. 1 and 2, an analyte collecting device 10 according to an embodiment of the present disclosure may include, in a broad meaning, a case 100 and a piston 200. The case 100 and the piston 200, for example, may be made of any one material of plastic, rubber, ceramic, an inorganic compound, and metal, or a combination thereof. The case 100 andthe piston 200, for example, may be manufactured through processes such as blow molding, compression molding, extrusion molding, injection molding, laminating, reaction injection molding, matrix molding, rotational molding, spin casting, transfer molding, thermoforming, and 3D printing. Further, the case 100 and the piston 200 can be manufactured in a large quantity, for example, as disposables by an existing automated facility. Further, the case 100 and the piston 200 may be individually manufactured and assembled to be provided.
The case has a space 102 therein in which a sample can be put, a piston 200 may be
inserted and assembled to each other in the internal space 102. The internal space 102 of the
case 100 may be provided in a shape with one side thereof opened and may be formed in a
shape corresponding to the piston 200 so that the piston 200 can be inserted and reciprocated
in the internal space 102. Further, the internal space 102 of the case 100 may be divided into
a plurality of sections by partition walls 230 of the piston 200. For example, the internal space
102 of the case 100 may be divided into a total of four sections 102a, 102b, 102c, and 102d,
but the spirit of the present disclosure is not limited thereto.
A sample that is put into the internal space 102 may be liquid, solid, of a mixture
including some or all of a cell, a virus, a tissue, exosome, protein, nucleic acid, an antigen, and
an antibody, and for example, may be a specimen taken from a human body. When the sample
that is put into the internal space 102 is a specimen taken from a human body, for example, the
nucleic acid in cells existing in the sample may be refined using the analyte collecting device
10.
Further, the internal space 102 may be filled with a solution containing a magnetic
substance and the plurality of sections may be filled with different solutions. For example,
when the internal space 102 is divided into a total of four sections 102a, 102b, 102c, and 102d, the first section 102a may be formed closest to the case 100 formed such that the piston 200 can be inserted therein, among the four sections 102a, 102b, 102c, and 102d, and the first section 102a may be filled with a solution for binding at least a portion of the analyte, which is in a biomaterial contained in the sample, to a magnetic substance through dissolution of the biomaterial.
For example, an analyte that is collected by the analyte collecting device 10 may be
nucleic acid, protein, vesicle (exosome, etc.), lipid, a carbohydrate, a cell (a blood cell, an
immunocyte, an oncocyte, pathogenic bacteria, etc.) and may include a biomaterial itself
contained in a sample or a substance that can be physically and/or chemically separated from
the biomaterial. Further, for example, when the nucleic acid in cells existing in a sample is
refined by the analyte collecting device 10, the analyte that is collected by the analyte collecting
device 10 may include nucleic acid.
The solution that fills the first section 102a may include, for example, at least one of a
lysis/binding buffer and 2-Propanol, and more particularly, may be provided as a solution
including some or all of magnetic nano/micro particles, salts (ex. Tris-HCl), chelating agent
(ex. ethylenediaminetetraacetic acid (EDTA)), a detergent (ex. Sodium dodecyl sulfate (SDS)
and Triton X-100), a reductant (ex. Dithiothreitol (DTT), a chaotropic agent (ex. Guanidine
thiocyanate), enzyme (ex. Proteinase K), alcohol (ex. 2-Propanol), and distilled water.
Further, the second section 102b is formed adjacent to the first section 102a with one
of the plurality of partition walls 230 therebetween, and the second section 102b may be filled
with a solution that enables washing of at least a portion of the analyte bound to a magnetic
substance.
The solution that fills the second section 102b, for example, may include washing
buffer, and more particularly, may be provided as a solution including some or all of diethyl
pyrocarbonate (DEPC), sodium citrate tribasic dehydrate, alcohol (ex. Ethanol, 2-propanol),
and distilled water.
Further, the third section 102c is formed adjacent to the second section 102b with one
of the plurality of partition walls 230 therebetween, and the third section 102c may be filled
with a solution that enables at least a portion of the analyte bound to a magnetic substance to
be eluted from the magnetic substance.
The solution that fills the third section 102c, for example, may include an elution buffer,
and more particularly, may be provided as a solution including some or all of salts (ex. Tris
HCl), a chelating agent (ex. Ethylenediaminetetraacetic acid (EDTA), diethyl pyrocarbonate
(DEPC), and distilled water.
Further, the fourth section 102d may be formed adjacent to the third section 102c with
one of the plurality of partition walls 230 therebetween and may be formed in contact with an
inner end positioned opposite to the opening of the case 100. The fourth section 102d may
be filled with gas such as air.
Meanwhile, the case 100 may include a specimen injection port 110, an aggregating
groove 120, a blowback portion 130, an exhaust port 140, a bed 150, and wings 160.
The specimen injection port 110 may include an injection hole 112 formed at the case
100 to connect the internal space 102 and the outside of the case 100 so that a sample can be
injected, and may further include a cap 114 sealing the injection hole 112 by selectively
covering the injection hole 112.
The injection hole 112 may be formed in a shape of which the top is wide and is
narrowed downward, and for example, may be formed on a conical shape, but the spirit of the
present disclosure should not be construed as being limited to the shape of the injection hole
112. Further, the injection hole 112 may be recessed with respect to a top surface of the case
100 so that the lower end of the injection hole 112 may communicates with the internal space
102. However, this is only an example and the injection hole 112 may be formed on a side
surface or the bottom surface other than the top surface of the case 100.
Further, as the piston 200 is moved in one direction in the internal space 102, the lower end of the injection hole 112 can be sequentially communicated with the sections 102a, 102b,
102c, and 102d. When a sample is injected through the injection hole 112, basically, the
sample may be injected while the injection hole 112 communicates with the first section 102a,
and the first stage of the refinement process may be performed immediately after the sample is
injected into the first section 102a.
The cap 114 may be made of an elastic material, for example, rubber, and the lower
end thereof is formed to correspond to the shape of the injection hole 112 such that when the
lower end is inserted in the injection hole 112, the cap can completely cover and seal the
injection hole 112. The cap 114 can prevent external foreign substances from permeating into
the internal space 102 by sealing the injection hole 112 when the device is not used, and the
cap 114 maybe separated from the injection hole 112 and the injection hole 112 maybe opened
so that a sample can be injected into the injection hole 112. After a sample is injected through
the injection hole 112, the cap 114 is coupled again, so the injection hole 112 can be sealed.
Accordingly, it is possible to prevent foreign substances from permeating into the internal space
not only before processing, but while processing is performed.
The aggregating groove 120 is recessed from an inner wall forming the internal space
102, and when a magnetic force is applied toward the aggregating groove 120 from the outside,
the magnetic substance in the internal space 102 can be aggregated in the aggregating groove
120. In this case, dissolution and a binding action of the sample occur in the first section 102a,
so when the magnetic substance and another biomaterial are bound, the magnetic substance
and the material bound to the magnetic substance can be aggregated in the aggregating groove
120.
Further, the aggregating groove 120 may be formed on an inner wall of the case 100
not to interfere with the injection hole 112, and in this embodiment, the aggregating groove
120 is exemplified as being form on the bottom surface of the internal space 102 of the case,
but the spirit of the present disclosure is not limited thereto. For example, the aggregating groove 120 may be formed on a side surface or the top surface of the internal space 102.
Further, the aggregating groove 120 may be formed as a semispherical shape, but the spirit of
the present disclosure is not construed to be limitative due to the shape of the aggregating
groove 120, and the aggregating groove 120 may be formed in other shapes such as a conical
shape and a hexahedron shape, depending on cases.
Further, the aggregating groove 120 may be formed on the same line as the injection
hole 112. Alternatively, the aggregating groove 120 may be formed at a position within a
range in which the aggregating groove 120 and the injection hole 112 both can be
communicated with at least the first section 102a. Accordingly, a sample injected through the
injection hole 112 can be accommodated and aggregated in the aggregating groove 120 without
additional movement of the piston 200. However, this is only an example, and even if the
aggregating groove 120 is formed at a position where the aggregating groove 120 cannot be
communicated with both of the injection hole 112 and any one of the sections 102a, 102b, 102c,
and 102d, a sample can be aggregated by the additional movement of the piston 200.
The blowback portion 130 is provided at an end opposite to a side in which the piston
200 is inserted and includes a flow hole 132 having both ends communicated with the internal
space. Further, the blowback portion 130 may be formed on the top of the case 10, but the
spirit of the present disclosure is not limited thereto, and the blowback portion 130 may be
formed on aside orthebottom of the case 100. When the piston 200 is moved forward toward
the blowback portion 130, the gas such as air existing in the fourth section 102d is blown back
to the flow hole 132 by the blowback portion 130, so the analyte that finishes being refined and
exists in the third section 102c can be discharged through the exhaust port 140 and collected in
a collection container X (see, FIG. 3).
To this end, the flow hole 132 includes a flow hole inlet 1322, a bridge 1324, and a
flow hole outlet 1326. Each of the flow hole inlet 1322 and the flow hole outlet 1326 is
formed such that an end thereof is communicated with the internal space 102, and other end thereof is connected through the bridge 1324, so that the entire flow hole 132 may be formed as an U-shaped channel. In this case, the flow hole inlet 1322 may be formed closer to the end opposite to the opening of the internal space 102 than the flow hole outlet 1326.
Accordingly, when the piston 200 is moved forward to narrow the fourth section 102d, the gas
such as air in the fourth section 102d flows into the flow hole inlet 1322 by pressure, and can
pass through the bridge 1324 and the flow hole outlet 1326 and then flow into the third section
102c adjacent to the fourth section 102d. The analyte accommodated in the third section 102c
can be pushed through the exhaust port 140 by the pressure of the inflow gas and can be
discharged from the case 100. The analyte discharged as described above can be collected in
the collection container X coupled to a container coupling protrusion 142 to be described below.
Due to the configuration of the blowback portion 130, a user can finely adjust the
amount of gas that is blown back through the flow hole 132 by adjusting the degree of pressing
the piston 200. As the amount of the gas that is blown back is adjusted, it is possible to finely
adjust the amount of the analyte that is discharged through the exhaust port 140 and then
collected. As described above, according to the present disclosure, since it is possible to
finely adjust the amount of the collected analyte by finely adjusting the degree of pressing the
piston 200, the analyte collecting device 10 according to the present disclosure can be
especially and usefully used when performing an examination in which it is very important to
collect an analyte in a predetermined quantity.
Meanwhile, the flow hole 132 of the blowback portion 130 may be formed in a shape
that is open such that the top of the bridge 1324 is communicated with the outside.
Accordingly, a cover 134 for selectively sealing the open surface of the bridge 1324 may be
further provided. The cover 134 maybe made of an elastic material, for example, rubber, and
the lower end thereof is formed to correspond to the shape of the bridge 1324 such that when
the lower end of the cover 134 is inserted in the bridge 1324, the cover can completely cover
and seal the opening of the bridge1324. The cover 134 can prevent external foreign substances from permeating into the internal space 102 by sealing the flow hole 132, whereby external foreign substances can be prevented from permeating into the internal space 102 not only before processing, but while processing is performed.
In this embodiment, it is exemplified that the flow hole 132 of the blowback portion
130 is formed in an open shape to be communicated with the outside and the opening of the
flow hole 132 is sealed by the cover 134, but the spirit of the present disclosure is not limited
thereto. For example, the flow hole 132 may be formed in a shape such that the bridge 1342
is not open and is only communicated with the internal space 102 without being communicated
with the outside as it is.
The exhaust port 140 may be formed to be communicated with the outside at an end
opposite to the opening in which the piston 200 is inserted, and may be formed such that a
sample that has undergone predetermined processing in the internal space 102 can be
discharged as an analyte from the case 100. To this end, the exhaust port 140 may be formed
through one side of the case 100, and as shown in FIG. 2, may be formed at a position facing
the blowback portion 130. However, this is only an example and the exhaust port 140 may
be formed at a position not facing the blowback portion 130. Further, in this embodiment,
although it is exemplified that the exhaust port 140 is formed on the bottom of the internal
space 120, the spirit of the present disclosure is not limited thereto and the exhaust port may
be formed on a side or the top of the internal space 102. In this case, when an analyte is
discharged through the exhaust port 140, a force by gravity is not applied but the analyte can
be collected by the pressure due to the blowback phenomenon.
Further, the container coupling protrusion 142 may be formed at the portion where the
exhaust port 140 of the case 100 is formed. The container coupling protrusion 142 may
protrude from the outer surface of the case 100 and may be formed in a shape extending the
exhaust port 140 to the outside of the case 100. Further, the container coupling protrusion
142 may be formed in a shape that can be fastened to the collection container X, and the container coupling protrusion 142 and the collection container X can be fastened to each other by fitting, bolting, or the like. The collection container X may be made of soft plastic, but the spirit of the present disclosure is not limited thereto.
The bed 150 may be formed in the bottom of the case 100 and may be held by a holder
to be described below so that the analyte collecting device 100 can be mounted thereon.
Further, wings 160 maybe formed a both sides of the bed 150. The wings 160 maybe formed
in a shape protruding throughout the entire width of sides of the case 100 and support both
sides of the holder 20 when the bed 150 is held by the holder 20, so the case 100 can be stably
held and fixed on the holder 20 by interference of the holder 20 and the wings 160.
Further, a label (not shown) may be attached to sides of the wings 160 or the sides may
be used as spaces for writing texts. Accordingly, the analyte collecting apparatus 10 can be
systemically managed.
The piston 200 may be provided to be inserted into the internal space 102 through the
opening formed at the case 100 and may be provided to reciprocate in the internal space 102.
Further, the piston 200 includes at least one partition wall 230 dividing the internal space 102.
Further, the piston 200 may further include a center column 210, a piston head 200, a flange
240 and a sealing member 250.
The center column 210, for example, may be provided in a cylindrical shape and is
provided to connect the piston head 220 and the partition wall 230. Further, the column 210
may connect a plurality of partition walls 230, and the portion connecting the piston head 220
and the partition wall 230 and the portion connecting the plurality of partition walls 230 may
be different in thickness. For example, the thickness of the portion connecting the piston head
220 and the partition wall 230 may be set larger than the thickness of the portion connecting
the plurality of partition walls 230, and accordingly, the column 210 may occupy the spaces of
the sections 102a, 102b, 102c, and 102d in a limitative manner. However, this is only an
example, and the center column 210 may have entirely the same thickness, or the thickness of the portion connecting the plurality of partition walls 230 may be set larger than the thickness of the portion connecting the piston head 220 and the partition wall 230.
The piston head 220 may be connected to an end of the center column 210, and may
be provided to be selectively clamped by a clamp 510 of the plunger 50 to be described below.
Further, the piston head 220 may be formed in a disc shape having a radius larger than the
center column 210, and may be provided as a flange shape for the center column 210.
The partition walls 230 may radially extend from the circumferential surface of the
center column 210 to be spaced apart from each other. Further, fourth partition walls 230 may
be provided and can divide the internal space into a total of four sections 102a, 102b, 102c, and
102d, but the spirit of the present disclosure is not limited thereto, and a certain number of
partition walls may be provided, if necessary.
For example, referring to FIG. 5, an analyte collecting device 10' according to another
embodiment of the present disclosure is proposed. In this embodiment, unlike the
embodiment described above, the number of partition walls 230 may be two, and accordingly,
the number of sections 102a and 102b may be two. According to the analyte collecting device
'having this configuration, only processing by one solution may be performed in the internal
space 102 and a plurality of devices may be required for processing that requires a plurality of
stages. However, there is the advantage that the size of the devices is correspondingly
decreased, the manufacturing cost is reduced, and the devices can be easily used when only
one processing is required. However, the spirit of the present disclosure is not limited to the
numbers of the partition walls and the sections, and three or more partition walls and sections
may be formed, depending on cases.
Referring to FIG. 2 again, the flange 240 may be attached to at least one surface of two
surfaces of the partition wall 230 and may be provided such that the circumferential surface is
closer to the inner wall forming the internal space 102 of the case 100 than the circumferential
surface of the partition wall 230. For example, when the flange 240 and the partition wall are provided in circular disc shapes, the radius of the flange 240 may be larger than the radius of the partition wall 230. Due to this shape of the flange 240, when two flanges 240 are attached to two sides of the partition wall 230, respectively, a space may be formed along the circumference of the partition wall 230 between the two flanges 240. The sealing member
250 may be provided in the space formed in this way.
The sealing member 250 may surround the circumferential surface of the partition wall
230 and may be in contact with the inner wall of the case 100. The sealing member may be
an O-ring made of a material such as rubber. By the sealing member 250, the gap between
the partition wall 230 and the inner wall of the case 100 can be sealed by the sealing member
250 and the substances accommodated in the sections 102a, 102b, 102c, and 102d can be
prevented from leaking from the corresponding sections. Further, even though the piston 200
is moved in the internal space 102, the sealing member 250 may be interfered with by the flange
240, so that the sealing member 250 can keep positioned between the two flanges 240 without
separating from the circumferential surface of the partition wall 230.
A process of collecting a sample as an analyte through a process such as refinement
and a process of performing a predetermined examination on the collected analyte can be
manually performed by the analyte collecting device 100 having the configuration described
above, and may be automatically performed by a system 1 for examining an analyte. When
the system 1 for examining an analyte is used, precise control is possible, as compared with
manual work, so collection and examination of an analyte can be more systematically
performed. Hereafter, the system 1 for examining an analyte according to an embodiment of
the present disclosure is described.
Referring to FIG. 3, the system 1 for examining an analyte according to an embodiment
of the present disclosure may be configured to not only perform a predetermined examination
on an analyte collected through the analyte collecting device 10, but perform processing, such
as refinement, and collection on the analyte. However, depending on cases, the system 1 for examining an analyte may also be configured to perform only processing, such as refinement, and collection on an analyte.
The system 1 for examining an analyte may include a holder 20, an aggregating device
, a de-aggregating device 40, a plunger 50, and a controller 60.
The holder 20 may be provided to separably hold the analyte collecting device 10.
Further, the top of the holder 20 may have a shape that can be fastened to the bed 150 of the
analyte collecting device 10. The holder 20 may have a width and a shape that can be inserted
in the space between two wings 160. Accordingly, a process for collecting an analyte of the
analyte collecting device 10 may be performed with the analyte collecting device 10 mounted
by the holder 20.
The aggregating device 30 may be provided to selectively apply a magnetic force
toward the aggregating groove 120 of the analyte collecting device 10 so that the magnetic
substance in a solution accommodated in the internal space 102 and a substance binding to the
magnetic substance can be aggregated in the aggregating groove 120. The aggregating device
may be controlled by the controller 60 to be described below and may be provided as a
member such as an electromagnet that can be supplied with power and can generate a magnetic
force. Further, the aggregating device 30 may be provided inside the holder 20, and may be
disposed at a position close to the aggregating groove 120 when the analyte collecting device
is mounted in the holder 20.
The de-aggregating device 40 may be provided to selectively apply a magnetic force
the internal space 102. The de-aggregating device 40 may be controlled by the controller 60
and may be provided as a member such as an electromagnet that can be supplied with power
and can generate a magnetic force. Further, the de-aggregating device 40 may be disposed
adjacent to a side of the top of the case 100, and may be operated to apply a magnetic force to
the internal space 102 of the case 100 using power supplied from the outside. Accordingly,
the controller 60 controls the de-aggregating device 40 with a magnetic substance aggregated in the aggregating groove 120, whereby the magnetic substance aggregated in the aggregating groove 120 and the substance bound to the magnetic substance can be separated by the magnetic force applied to the internal space 102, and accordingly, the substance binding to the magnetic substance can float back into the internal space 102.
The plunger 50 may translate the piston 200 in the internal space 102 by pushing or
pulling the piston head 220. To this end, the plunger 50 may include a clamp 510 that can
selectively hold the piston head 220. The clamp 510 may have a shape corresponding to the
shape of the piston head 220, and may be provided to selectively hold the piston head 220.
The controller 60 may be provided to control the components of the system 1 for
examining an analyte. In detail, the controller 60 may be provided to control at least one or
more of the holder 20, the aggregating device 30, the de-aggregating device 40, and the plunger
50. The controller 60, for example, may be a small built-in computer and may include
programs, a memory, and a CPU that is a data processing unit. The programs may include an
algorithm for controlling at least one or more of the holder 20, the aggregating device 30, the
de-aggregating device 40, and the plunger 50. Further, the programs may be stored in a
computer memory medium, for example, a memory such as a flexible disc, a compact disc, a
hard disc, and a magneto-optical disk (MO), and may be installed in the controller 60. For
example, predetermined processing that is performed in the analyte collecting device 10 by the
system 1 for examining an analyte may include a plurality of stages, and, as the controller 60
controls the operation of the plunger 50, the plurality of stages can be sequentially performed.
Hereafter, the plurality of stages that is performed by the analyte collecting device 10
and the system 1 for examining an analyte is described with reference to FIGS. 4A to 4J. In
the following description, although it is exemplified that an analyte that is collected by the
analyte collecting device 10 is a refined nucleic acid and includes a specimen for Polymerase
Chain Reaction (PCR), the spirit of the present disclosure is not limited thereto, and the analyte
collecting device and the system for examining an analyte may be used to collect other kinds of analytes.
First, a sample can be put into the internal space 102 of the analyte collecting device
(see,FIG.4A). The sample that is put into the internal space 102 maybe liquid, solid, or
a mixture including some or all of a cell, a virus, a tissue, exosome, protein, nucleic acid, an
antigen, and an antibody, and for example, may be a specimen taken from a human body.
Next, the piston 200 is moved in the internal space 102 and predetermined processing
including a plurality of stages can be sequentially performed. The stage in which
predetermined processing is sequentially performed is described through an example as follows.
First, a biomaterial contained in a sample is dissolved by the solution in the first section 102a
and nucleic acid in the biomaterial can be bound to a magnetic substance (see, FIG. 4B).
Thereafter, the controller 60 applies a magnetic force to the aggregating groove 120 by
controlling the aggregating device 30, and the magnetic substance bound to the nucleic acid
can be aggregated in the aggregating groove 120 (see, FIG. 4C). After the magnetic substance
bound to the nucleic acid is aggregated in the aggregating groove 120, the controller 60 pushes
backward the piston 200 such that the second section 102b is disposed over the aggregating
groove 120 (see, FIG. 4D).
After finishing moving the piston 200, the controller 60 can remove the magnetic force
applied to the aggregating groove 120 by stopping operation of the aggregating device 30 and
can apply a magnetic force to the internal space 102 by operating the de-aggregating device 40
(see, FIG. 4E). Accordingly, the nucleic acid and the magnetic substance bound to each other
in the aggregating groove 120 can be separated, and the magnetic substance bound to the
nucleic acid can float into the internal space of the case. Thereafter, the nucleic acid bound
to the magnetic substance can be started to be refined by the solution in the second section
102b.
After a predetermined time for which the nucleic acid can finish being refined passes,
the controller 60 can aggregate the nucleic acid bound to the magnetic substance in the aggregating groove 120 by applying a magnetic force to the aggregating groove 120 by operating the aggregating device 30 again (see, FIG. 4F). Thereafter, the controller 60 moves backward the piston 200 by operating the plunger 50, whereby the third section 102c can be disposed over the aggregating groove 120 (see, FIG. 4G).
After finishing moving the piston 200, the controller 60 can remove the magnetic force
applied to the aggregating groove 120 by stopping operation of the aggregating device 30 and
can apply a magnetic force to the internal space 102 by operating the de-aggregating device 40
(see, FIG. 4H). Accordingly, the nucleic acid and the magnetic substance bound to each other
in the aggregating groove 120 can be separated, and the magnetic substance bound to the
nucleic acid can float into the internal space of the case 100. Thereafter, a process in which
the nucleic acid bound to the magnetic substance is eluted from the magnetic substance by the
solution in the third section 102c can be performed.
After a predetermined time for which the eluting of the nucleic acid is completed
passes, the controller 60 can aggregate the magnetic substance separated from the nucleic acid
in the aggregating groove 120 by applying a magnetic force to the aggregating groove 120 by
operating the aggregating device 30 again (see, FIG. 41). Thereafter, the controller may allow
the piston 200 to be pushed toward the end of the internal space 102 in the internal space 102,
thereby pushing the third section 102c toward the blowback portion 130 containing the eluted
nucleic acid. Further, the nucleic acid pressed toward the blowback 130 can be discharged as
an analyte through the exhaust port 140 formed at the case 100 (see, FIG. 4J). In this case,
the collecting container X may have been fastened to the container coupling protrusion 142,
and the analyte discharged through the exhaust port 140 can be collected in the collecting
container X and used for a predetermined examination procedure such as PCR.
Meanwhile, the system 1 for examining an analyte, as shown in FIG. 3, may be
configured such that a single system is controlled by one controller 60, but, as shown in FIG.
6, a plurality of systems may be controlled. In other words, as in the analyte collecting device
' shown in FIG. 5, when only two sections are provided and one section is filled with a
solution for one processing, a plurality of analyte collecting devices 10 may be required to
perform processing that should be performed through a plurality of stages. As described
above, when it is required to operate a plurality of analyte collecting devices in one process, a
system ' for examining an analyte shown in FIG. 6 may be used.
Hereafter, the system 1' for examining an analyte according to another embodiment of
the present disclosure is described.
Referring to FIG. 6, the system 1' for examining an analyte may include a plurality of
analyte collecting devices 10 and may include holders 20, aggregating devices 30, de
aggregating devices 40, a plunger 50 to correspond to the number of the analyte collecting
devices 10, and may further include conveying lines 70 connecting the analyte collecting
devices 10, respectively. In this case, the conveying line 70 may be provided to connect the
exhaust port 140 of any one analyte collecting device 10 and the injection hole 112 of another
analyte collecting device 10. Accordingly, an analyte processed through the analyte
collecting device 10 at the front end is injected into the analyte collecting device 10 at the rear
end through the conveying line 70, and following processing can be performed.
Further, although not shown in this embodiment, the conveying line 70 may further
have members such as a pump and a valve for conveying fluid. Further, the conveying line
may be separably fastened to the analyte collecting device 10, whereby the conveying line
can be fastened to the analyte collecting device 10 or the position thereof can be changed,
if necessary. However, this is only an example, and the conveying line 70 may be omitted
and the analyte discharged from the analyte collecting device 10 at the front end may be
manually conveyed to the analyte collecting device 10 at the rear end.
The controller 60 may include a plunger module 610, a de-aggregating module 620,
and an aggregating module 630. The plunger module 610 maybe connected to the plungers
to independently control the plungers 50. Further, the de-aggregating module 620 may be connected to the de-aggregating devices 40 to independently control the de-aggregating devices
40. Further, the aggregating module 630 may be connected to the aggregating devices 30 to
independently control the aggregating devices 30. The plunger module 610, the de
aggregating module 620, and the aggregating module 630 may be provided as independent
algorithms stored in a chipset module or one chipset disposed in the controller 60.
According to the system 1' for examining an analyte described above, a plurality of
systems can be independently controlled, so there is the advantage that the system 1' can be
especially and usefully used when a process should be performed through multiple stages.
Meanwhile, the configuration of the controller 60 of the system for examining an
analyte may be changed in various ways. For example, the configuration shown in FIG. 7 is
also possible. Hereafter, the system 1" for examining an analyte according to another
embodiment of the present disclosure is described with reference to FIG. 7. In the following
description, the system 1" for examining an analyte has a difference in the configuration of the
controller 60, as compared with the system 1' for examining an analyte described above, so the
difference is mainly described and the system 1' for examining an analyte described above is
referred to for the same configuration.
Referring to FIG. 7, the controller 60 of the system 1" for examining an analyte may
have a configuration in which modules correspond to systems, respectively, each of which
includes one analyte collecting device 10, one holder 20, one aggregating device 30, one de
aggregating device 40, and one plunger 50. For example, when a total of three systems each
including one analyte collecting device 10, one holder 20, one aggregating device 30, one de
aggregating device 40, and one plunger 50 are provided, the controller 60 may include a first
module 602, a second module 604, and a third module 606 that independently control the
systems. Accordingly, there is the advantage that it is possible to easily change the number
of low-class systems included in the entire system by removing some of the modules in the
controller 60 or adding a new module.
Hereafter, the operation and effects of the analyte collecting devices 10 and 10' and
the systems 1, 1', and 1" for examining an analyte having the configurations described above
in accordance with embodiments of the present disclosure are described. The applicant of the
present disclosure performed examinations in accordance with an experimental example to be
described below to prove the effects of the present disclosure.
(Experimental example)
Referring to FIGS. 8 and 9, in this experimental example, an analyte collecting device
including a first section 102a, a second section 102b, a third section 102c, and a fourth section
102d was prepared, the capacities of the first to third sections 102a, 102b, and 102c were set
as 750, 750, and 550 pL, and the examination was performed in this state. In this
experimental example, Influenza A HINI (Human, strain: KUMC-76) cDNA was conveyed
and PCR of the conveyed resultant was performed using the analyte collecting devices
according to the embodiments of the present disclosure, and then the result was measured.
The equipment used for PCR was QuantStudioTM 3 by ThermoFisher Scientific
(Applied Biosystems), and the reagent was PowerUp TM SYBRTM Green Master Mix by the
same company. The used primer information is as follows.
Inf.A_F: GACCRATCCTGTCACCTCTGAC (22 mer, 10 pM)
Inf.A_R: AGGGCATTYTGGACAAAKCGTCTA (24 mer, 10 pM)
PCR reaction volume was 20 pL, PowerUp TM SYBRTM Green Master Mix was 10 pL,
the primer was 1 L, and a reagent of 8 pL was included for each experiment.
The PCR procedure was composed of a total of three states. First, a hold stage was
performed under the condition of 50 °C/2 min. and 95 °C/10 min, and a cycling stage was
performed for 40 cycles under the condition of 95 °C/15 sec. and 60 °C/60 sec. Finallyina
melt curve stage, a result was measured while temperature was increased by 0.15 °C per 1
seconds after 95 °C/15 sec. and 60 °C/1 min.
Influenza A HINI (Human, strain: KUMC-76) cDNA that is a sample was produced by QuantiNovaTM Reverse Transcription Kit by Qiagen.
In the first section 102a, a solution and magnetic particles required for cell dissolution
(lysis) and binding of nucleic acid and magnetic particles were injected, and additionally,
Dynabeads@ MyOne TM Silane (ThermoFisher Scientific, 40 mg/mL) of 50 pL, Lysis/binding
Buffer of 300 pL by Dynabeads@ SILANE viral NA kit (ThermoFisher Scientific), 2-Propanol
(Sigma-Aldrich, 19516-500 mL) of 150 pL, Distilled water (DW) of 244.5 pL were injected.
In the second section 102b, a solution for washing was injected in advance, and
Washing Buffer2 of 750 pL by Dynabeads@ SILANE viral NA kit (ThermoFisher Scientific)
was injected.
In the third section 102c, a solution for nucleic acid elution was injected in advance,
and in the cartridge; Elution Buffer of 150 pL and DW of 400 pL by Dynabeads@ SILANE
viral NA kit (ThermoFisher Scientific) were additionally injected in the E.B. experiment shown
in FIGS. 8 and 9, and DW of 550 pL was injected in the cartridge, H 2 0 experiment shown in
FIGS. 8 and 9.
In the first section 102a of the analyte collecting device set as described above,
Influenza A HIN cDNA of 5.5 pL was injected as a sample. Thereafter, analytes were
collected in accordance with the methods of collecting an analyte according to the
embodiments described above, and PCR was performed by injecting an undiluted solution, a
1/10 diluted solution, and 1/100 diluted solution of 8 pL of the collected analytes were injected
in individual PCR containers.
As a contrastive group, the same specimen was made pass through the same solutions
as the solutions injected in the first section 102a and the second section 102b, and finally, pass
through the solution in which Elution Buffer of 150 pL and DW of 400 pL were injected.
Further, an examiner manually performed the experiment using a pipette (pipetting, E.B. of
FIGS. 8 and 9). Similarly, in the contrastive group, PCR was performed by injecting an
undiluted solution, a 1/10 diluted solution, and 1/100 diluted solution of 8 pL of an analyte in individual PCR containers.
As a standard substances in PCR, 1/10, 1/100, and 1/1000 diluted solutions
(Inf.A.cDNA of FIGS. 8 and 9) of 8 pL of Influenza A HN1 cDNA that is the same solution
as that injected in each experiment were injected in individual PCR containers, whereby PCR
was performed.
Since Influenza A HIN cDNA injected in each experiment was 5.5 pL and the
capacity of the third section 102c was 550 pL, when an analyte was 100% conveyed, it
corresponds to a 1/100 diluted solution of the standard.
Referring to FIG. 8, it was found that when Influenza A HN cDNA solution
conveyed using the analyte collecting devices, systems, and methods according to
embodiments of the present disclosure was amplified, the threshold cycle (Ct) values measured
in the cartridge, E.B. experiment and the cartridge, H 2 0 experiment were both similar to the Ct
values of the contrastive group of this experimental example and the 1/100 diluted solution of
the standard, and the diluted solutions showed the same tendency. The Ct values prove the
contrary of the initial cDNA before amplification, so it was determined that collection of an
analyte through the analyte collecting devices, systems, and methods according to
embodiments of the present disclosure was effective.
Further, referring to FIGS. 8 and 9, it was found that the experiment of injecting
distilled water in the third section 102c while using the analyte collecting devices, systems, and
methods according to embodiments of the present disclosure has the most similar Ct value to
the 1/100 of the standard and the initial concentrations before amplification were similar.
According to the analyte collecting devices and systems for examining an analyte
having the configurations described above in accordance with embodiments of the present
disclosure, there is an effect that the structures of the devices and the systems are simple, so
the cost is low and the devices and the systems can be implemented in a small size. Further,
a sample can be effectively processed through an automated process and it is possible to achieve a constant yield ratio of an analyte every time regardless of the skill of the users.
The examples of the present disclosure have been described above as specific
embodiments, but these are only examples, and the present disclosure is not limited thereto,
and should be construed as having the widest scope according to the technical spirit disclosed
in the present specification. A person skilled in the art may combine/substitute the disclosed
embodiments to implement a pattern of a shape that is not disclosed, but it also does not depart
from the scope of the present disclosure. In addition, those skilled in the art can easily change
or modify the disclosed embodiments based on the present specification, and it is clear that
such changes or modifications also belong to the scope of the present disclosure.
Claims (16)
1. An analyte collecting device comprising: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space; wherein the case further includes: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate an outside of the case so that a sample can be injected, and wherein the specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section is filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section is filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case.
2. The analyte collecting device of claim 1, wherein the case includes: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with the outside, a sample put in the internal space being discharged through the exhaust port to the outside of the case; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space.
3. The analyte collecting device of claim 1, wherein the solution filled in the first section includes at least one of a lysis/binding buffer and isopropyl alcohol (2-propanol), the solution filled in the second section includes a washing buffer, and the solution filled in the third section includes an elution buffer.
4. The analyte collecting device of claim 1, wherein the piston includes a center column, wherein the one or more partition walls include a plurality of partition walls spaced apart from each other and radially extend from the circumferential surface of the center column, and wherein the internal space is divided into a plurality of sections by the partition walls and at least some of the divided plurality of sections are filled with different solutions.
5. The analyte collecting device of claim 1, wherein the piston further includes: a flange attached to at least one of two surfaces of each of the one or more partition walls provided perpendicular to an insertion direction of the piston, the flange having a circumferential surface provided closer to an inner wall surrounding the internal surface of the case than the circumferential surface of each of the one or more partition walls; and a sealing member provided to surround the circumferential surface of each of the one or more partition walls and being in contact with the inner wall of the case.
6. The analyte collecting device of any one of claims 1 to 5, wherein an aggregating groove is recessed from an inner wall forming the internal space of the case, and the magnetic substance is aggregated in the aggregating groove when a magnetic force is applied toward the aggregating groove from the outside.
7. The analyte collecting device of claim 1, wherein the analyte that is collected by the analyte collecting device includes at least one of nucleic acid, protein, vesicle, lipid, a carbohydrate, a cell, and a substance separated therefrom.
8. A system for examining an analyte, the system comprising: an analyte collecting device including a case including an opening and an internal space, and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space through the opening of the case to reciprocate in the internal space; and a holder separably holding the analyte collecting device, wherein the case further includes: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate an outside of the case so that a sample can be injected, and wherein the specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section is filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section is filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case.
9. The system of claim 8, further comprising: a plunger for translating the piston in the internal space by pushing or pulling a head of the piston; and a controller, wherein the plunger is controlled by the controller.
10. The system of claim 9, wherein the case includes: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with the outside, a sample, which is put in the internal space and undergoes the predetermined processing, being discharged from the case as an analyte; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space, wherein the controller controls the plunger to push the piston toward the blowback portion such that the analyte is discharged through the exhaust port by a blowback phenomenon.
11. The system of claim 9 or 10, wherein the case has an aggregating groove recessed from an inner wall forming the internal space, and the system further comprises: an aggregating device configured to selectively apply a magnetic force toward the aggregating groove so that the magnetic substance is aggregated in the aggregating groove, the aggregating device being controlled by the controller.
12. The system of claim 11, further comprising: a de-aggregating device configured to selectively apply a magnetic force to the internal space and being controlled by the controller, wherein the controller controls the de-aggregating device with the magnetic substance aggregated in the aggregating groove so that a magnetic force is applied to the internal space and the magnetic substance aggregated in the aggregating groove is separated.
13. A method of collecting analyte using an analyte collecting device including: a case including an opening and an internal space; and a piston including one or more partition walls dividing the internal space into a plurality of internal spaces, the piston being inserted into the internal space of the case to reciprocate in the internal space, the method comprising: putting a sample in the internal space; and collecting the sample as an analyte by sequentially performing a predetermined processing including a plurality of stages while the piston is moved in the internal space; wherein the case further includes: a specimen injection port including an injection hole formed at the case to allow the internal space to communicate an outside of the case so that a sample can be injected, and wherein the specimen injection port further includes a cap sealing the injection hole by selectively covering the injection hole, wherein the one or more partition walls include four partition walls, the plurality of internal spaces includes a first section, a second section, a third section, and a fourth section sequentially divided by the four partition walls, the first section to the fourth section being sequentially disposed away from the opening of the case, the first section is filled with a solution that dissolves a biomaterial contained in the sample and binds at least a portion of an analyte in the biomaterial to a magnetic substance, the second section is filled with a solution that washes at least a portion of the analyte bonded to the magnetic substance, the third section is filled with a solution that elutes at least a portion of the analyte bonded to the magnetic substance from the magnetic substance, and the fourth section is formed adjacent to the third section and in contact with an inner end of the case.
14. The method of claim 13, wherein the case includes: an exhaust port formed at an end portion opposite to a side in which the piston is inserted such that the internal space communicates with the outside, the sample accommodated in the internal space being discharged through the exhaust port to the outside of the case; and a blowback portion provided at the opposite end portion to the side in which the piston is inserted, the blowback portion including a flow hole formed such that both ends thereof communicate with the internal space, wherein the method further includes discharging the analyte in which the piston moving in the internal space pushes a section contained an analyte undergoing the predetermined processing toward the blowback portion formed at an end portion of the case, and the analyte pressed through the blowback portion is discharged through an exhaust port formed at the case.
15. The method of claim 13 or 14, wherein an aggregating groove is recessed from an inner wall forming the internal space of the case, and wherein the method further includes aggregating the magnetic substance in the aggregating groove by applying a magnetic force to the aggregating groove.
16. The method of claim 15, wherein the first section of the four sections is formed closest to an opening of the case, the second section is formed adjacent to the first section with one of the partition walls therebetween, the third section is formed adjacent to the second section with one of the partition walls therebetween, and the fourth section is formed adjacent to the third section with one of the partition walls therebetween to be in contact with an inner end of the case, wherein the sample is put in the first section, and wherein the sequentially performing the predetermined processing further includes: bonding at least a portion of an analyte in a biomaterial contained in the sample to the magnetic substance by dissolving the biomaterial by the solution filled in the first section; disposing the second section above the aggregating groove by moving the piston backward after the magnetic substance bound to at least a portion of the analyte is aggregated in the aggregating groove by applying a magnetic force to the aggregating groove; floating the magnetic substance in the second section in which the magnetic force applied to the aggregating groove is removed, and the magnetic substance aggregated in the aggregating groove is separated and floats into the second section by applying a magnetic force to the internal space; washing at least a portion of the analyte bound to the magnetic substance by a solution filled in the second section; disposing the third section above the aggregating groove by moving the piston backward after the magnetic substance bound to at least a portion of the analyte is aggregated in the aggregating groove by applying a magnetic force to the aggregating groove; floating the magnetic substance in the third section in which the magnetic force applied to the aggregating groove is removed, and the magnetic substance aggregated in the aggregating groove is separated and floats into the third section by applying a magnetic force to the internal space; eluting at least a portion of the analyte bound to the magnetic substance from the magnetic substance by a solution filled in the third section; and aggregating the magnetic substance with at least a portion of the analyte eluted in the aggregating groove by applying a magnetic force to the aggregating groove.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020190044545A KR20200121681A (en) | 2019-04-16 | 2019-04-16 | Analyte collecting device, analyte collecting method using same and system for examining analyte |
| KR10-2019-0044545 | 2019-04-16 | ||
| PCT/KR2020/005045 WO2020213930A1 (en) | 2019-04-16 | 2020-04-14 | Analyte collecting device, and analyte collecting method and analyte inspection system using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2020258915A1 AU2020258915A1 (en) | 2021-10-14 |
| AU2020258915B2 true AU2020258915B2 (en) | 2023-09-28 |
Family
ID=72837493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020258915A Active AU2020258915B2 (en) | 2019-04-16 | 2020-04-14 | Analyte collecting device, and analyte collecting method and analyte inspection system using same |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20220187330A1 (en) |
| EP (1) | EP3940386A4 (en) |
| JP (1) | JP7301416B2 (en) |
| KR (1) | KR20200121681A (en) |
| CN (1) | CN113711051A (en) |
| AU (1) | AU2020258915B2 (en) |
| BR (1) | BR112021020767A2 (en) |
| CA (1) | CA3137151C (en) |
| WO (1) | WO2020213930A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102326826B1 (en) * | 2021-01-14 | 2021-11-16 | (주)얼라인드제네틱스 | Analyte examining apparatus and analyte examining method using the same |
| CN116953274A (en) * | 2022-04-19 | 2023-10-27 | 宋世平 | Sequential sample adding device and automatic sample adding system |
| CN117309496B (en) * | 2023-10-28 | 2024-03-01 | 河海大学 | River sediment sampling monitoring equipment |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140276039A1 (en) * | 2013-03-13 | 2014-09-18 | Medrad, Inc. | Multiple compartment syringe |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5324682U (en) * | 1976-08-09 | 1978-03-02 | ||
| US4326540A (en) * | 1979-11-06 | 1982-04-27 | Marquest Medical Products, Inc. | Syringe device with means for selectively isolating a blood sample after removal of contaminates |
| JPH10332687A (en) * | 1997-05-29 | 1998-12-18 | Fujirebio Inc | Method and device for capturing specific substance contained in liquid and method for detecting the specific substance utilizing the method |
| WO1999026724A2 (en) * | 1997-11-25 | 1999-06-03 | Mosaic Technologies | Devices and methods for detecting target molecules in biological samples |
| GB2425498A (en) | 2005-04-25 | 2006-11-01 | Dynal Biotech Asa | A magnetic separation device |
| CN101415813B (en) * | 2006-02-03 | 2013-04-10 | 微芯片生物工艺学股份有限公司 | Microfluidic devices |
| FR2939445B1 (en) * | 2008-12-10 | 2013-05-03 | Biomerieux Sa | AUTOMATED LYSE SYSTEM OF MICROORGANISMS PRESENT IN SAMPLE, NUCLEIC ACID EXTRACTION AND PURIFICATION OF MICROORGANISMS FOR ANALYSIS |
| JP5972174B2 (en) * | 2009-12-30 | 2016-08-17 | スリーエム イノベイティブ プロパティズ カンパニー | Detection method of living bioburden using microparticles |
| JP6254089B2 (en) * | 2011-10-20 | 2017-12-27 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | Syringe with a fragile plunger for arterial blood gas sampling |
| SG11201501550QA (en) | 2012-09-10 | 2015-03-30 | Biofire Diagnostics Llc | Multiple amplification cycle detection |
| KR20140068758A (en) * | 2012-11-28 | 2014-06-09 | 서울대학교산학협력단 | Nanoparticle separation using microfluidic chip and biomaterial assay method using the same |
| JP2016067275A (en) * | 2014-09-30 | 2016-05-09 | セイコーエプソン株式会社 | Device for purifying nucleic acid |
| JP2016067273A (en) * | 2014-09-30 | 2016-05-09 | セイコーエプソン株式会社 | Container assembly and container assembly kit |
| JP2016136852A (en) | 2015-01-26 | 2016-08-04 | セイコーエプソン株式会社 | Bio-related substance purification cartridge set |
| US20180371518A1 (en) | 2015-07-28 | 2018-12-27 | Kabushiki Kaisha Dnaform | Analysis kit and analysis method using same |
| KR20180098089A (en) * | 2017-02-24 | 2018-09-03 | (주)바이오니아 | The kit for biochemical analysis by assembling a purification catridge with a panel selected from various pcr amplification panels |
| US20220314215A1 (en) * | 2019-10-29 | 2022-10-06 | Hewlett-Packard Development Company, L.P. | Vertically layered fluid columns |
-
2019
- 2019-04-16 KR KR1020190044545A patent/KR20200121681A/en active Search and Examination
-
2020
- 2020-04-14 US US17/440,106 patent/US20220187330A1/en active Pending
- 2020-04-14 JP JP2021561652A patent/JP7301416B2/en active Active
- 2020-04-14 AU AU2020258915A patent/AU2020258915B2/en active Active
- 2020-04-14 WO PCT/KR2020/005045 patent/WO2020213930A1/en unknown
- 2020-04-14 BR BR112021020767A patent/BR112021020767A2/en unknown
- 2020-04-14 CA CA3137151A patent/CA3137151C/en active Active
- 2020-04-14 CN CN202080028476.XA patent/CN113711051A/en active Pending
- 2020-04-14 EP EP20791672.7A patent/EP3940386A4/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140276039A1 (en) * | 2013-03-13 | 2014-09-18 | Medrad, Inc. | Multiple compartment syringe |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20200121681A (en) | 2020-10-26 |
| CN113711051A (en) | 2021-11-26 |
| CA3137151A1 (en) | 2020-10-22 |
| JP2022529942A (en) | 2022-06-27 |
| EP3940386A4 (en) | 2022-12-28 |
| CA3137151C (en) | 2024-03-05 |
| AU2020258915A1 (en) | 2021-10-14 |
| BR112021020767A2 (en) | 2021-12-14 |
| WO2020213930A1 (en) | 2020-10-22 |
| EP3940386A1 (en) | 2022-01-19 |
| JP7301416B2 (en) | 2023-07-03 |
| US20220187330A1 (en) | 2022-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020258915B2 (en) | Analyte collecting device, and analyte collecting method and analyte inspection system using same | |
| EP2440941B1 (en) | Sheath flow devices and methods | |
| CN107099445B (en) | Test cartridge with integrated transport module | |
| CN111841677A (en) | Systems and methods for collecting nucleic acid samples | |
| US20230356213A1 (en) | Analyte inspection apparatus and analyte inspection method using same | |
| US20230001407A1 (en) | Microporous substrate for use in a disposable bioassay cartridge | |
| RU84381U1 (en) | DEVICE FOR AUTOMATED ISOLATION OF NUCLEIC ACIDS | |
| WO2007055165A1 (en) | Method of separating nucleic acid, microreactor for testing nucleic acid and nucleic acid test system | |
| JP2014124097A (en) | Cartridge for analysis of nucleic acid and device for analysis of nucleic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |