AU2003300462A1 - Method for detecting and limiting breast carcinoma risk by a micronucleus test of binuclear cells - Google Patents
Method for detecting and limiting breast carcinoma risk by a micronucleus test of binuclear cellsInfo
- Publication number
- AU2003300462A1 AU2003300462A1 AU2003300462A AU2003300462A AU2003300462A1 AU 2003300462 A1 AU2003300462 A1 AU 2003300462A1 AU 2003300462 A AU2003300462 A AU 2003300462A AU 2003300462 A AU2003300462 A AU 2003300462A AU 2003300462 A1 AU2003300462 A1 AU 2003300462A1
- Authority
- AU
- Australia
- Prior art keywords
- determining
- risk
- hours
- culture
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Determining the risk of developing breast cancer based on spontaneous development of micronuclei in mitogen-treated lymphocyte cultures, is new. Method for determining the risk of developing breast cancer comprising: (a) establishing a lymphocyte culture from heparin-treated peripheral blood; (b) mitogenic stimulation within 6 hours of establishing the culture; (c) adding a cytokinesis inhibitor 40-50 hours after establishment; (d) interrupting culture 12-24 hours after (c) by centrifuging, hypotonic treatment then preparation of a fixed cell suspension; (e) making a cytological preparation; (f) determining the micronucleus rate (MNR) in binuclear cells; and (g) determining the risk by comparing spontaneous MNR in the test sample with that in controls.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10246811A DE10246811B4 (en) | 2002-10-08 | 2002-10-08 | Method for detecting and limiting the risk of breast cancer by the micronucleus test on binucleated cells |
DE10246811.7 | 2002-10-08 | ||
PCT/EP2003/011141 WO2004034055A2 (en) | 2002-10-08 | 2003-10-08 | Method for detecting and limiting breast carcinoma risk by a micronucleus test of binuclear cells |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2003300462A8 AU2003300462A8 (en) | 2004-05-04 |
AU2003300462A1 true AU2003300462A1 (en) | 2004-05-04 |
Family
ID=32038312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2003300462A Abandoned AU2003300462A1 (en) | 2002-10-08 | 2003-10-08 | Method for detecting and limiting breast carcinoma risk by a micronucleus test of binuclear cells |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1549949B1 (en) |
AT (1) | ATE458197T1 (en) |
AU (1) | AU2003300462A1 (en) |
DE (2) | DE10246811B4 (en) |
WO (1) | WO2004034055A2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100410368C (en) * | 2005-07-19 | 2008-08-13 | 翁炳焕 | Pretreating method for chromosome nuclear type analysis lymphocyte culture specimen |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AUPQ943700A0 (en) * | 2000-08-16 | 2000-09-07 | Diatech Pty Ltd | Micronucleus assay |
-
2002
- 2002-10-08 DE DE10246811A patent/DE10246811B4/en not_active Expired - Fee Related
-
2003
- 2003-10-08 AT AT03756478T patent/ATE458197T1/en not_active IP Right Cessation
- 2003-10-08 DE DE50312427T patent/DE50312427D1/en not_active Expired - Lifetime
- 2003-10-08 AU AU2003300462A patent/AU2003300462A1/en not_active Abandoned
- 2003-10-08 EP EP03756478A patent/EP1549949B1/en not_active Expired - Lifetime
- 2003-10-08 WO PCT/EP2003/011141 patent/WO2004034055A2/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
DE10246811B4 (en) | 2005-12-29 |
AU2003300462A8 (en) | 2004-05-04 |
WO2004034055A2 (en) | 2004-04-22 |
DE10246811A1 (en) | 2004-04-22 |
EP1549949B1 (en) | 2010-02-17 |
ATE458197T1 (en) | 2010-03-15 |
WO2004034055A3 (en) | 2004-06-10 |
DE50312427D1 (en) | 2010-04-01 |
EP1549949A2 (en) | 2005-07-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase |