AU2001257091A1 - Aptamer based two-site binding assay - Google Patents

Aptamer based two-site binding assay

Info

Publication number
AU2001257091A1
AU2001257091A1 AU2001257091A AU5709101A AU2001257091A1 AU 2001257091 A1 AU2001257091 A1 AU 2001257091A1 AU 2001257091 A AU2001257091 A AU 2001257091A AU 5709101 A AU5709101 A AU 5709101A AU 2001257091 A1 AU2001257091 A1 AU 2001257091A1
Authority
AU
Australia
Prior art keywords
binding assay
site binding
aptamer based
aptamer
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001257091A
Inventor
Jim Heil
Sumedha Jayasena
Yun Lin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gilead Sciences Inc
Original Assignee
Gilead Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilead Sciences Inc filed Critical Gilead Sciences Inc
Publication of AU2001257091A1 publication Critical patent/AU2001257091A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/7056Selectin superfamily, e.g. LAM-1, GlyCAM, ELAM-1, PADGEM
    • G01N2333/70564Selectins, e.g. CD62
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974Thrombin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2001257091A 2000-04-18 2001-04-18 Aptamer based two-site binding assay Abandoned AU2001257091A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19801600P 2000-04-18 2000-04-18
US60198016 2000-04-18
PCT/US2001/012614 WO2001079562A1 (en) 2000-04-18 2001-04-18 Aptamer based two-site binding assay

Publications (1)

Publication Number Publication Date
AU2001257091A1 true AU2001257091A1 (en) 2001-10-30

Family

ID=22731642

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001257091A Abandoned AU2001257091A1 (en) 2000-04-18 2001-04-18 Aptamer based two-site binding assay

Country Status (3)

Country Link
US (1) US20020037506A1 (en)
AU (1) AU2001257091A1 (en)
WO (1) WO2001079562A1 (en)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
WO2003070984A1 (en) * 2002-02-15 2003-08-28 Somalogic, Inc. Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US6858390B2 (en) * 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
CA2483408A1 (en) * 2002-04-29 2003-11-13 Affibody Ab Sandwich assay and kit
EP1546380A4 (en) * 2002-05-28 2007-02-14 Us Genomics Inc Methods and apparati using single polymer analysis
AU2003251986A1 (en) * 2002-07-17 2004-02-02 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
WO2004013628A1 (en) * 2002-08-02 2004-02-12 Applera Corporation Fluorescence polarization assay
JP5179011B2 (en) 2002-10-09 2013-04-10 ディーエムアイ バイオサイエンシズ インコーポレイテッド Monitoring placental ischemia and multiple organ failure
SG153699A1 (en) * 2003-01-16 2009-07-29 Caprotec Bioanalytics Gmbh Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
EP1586068B1 (en) * 2003-01-23 2008-07-30 U.S. Genomics, Inc. Methods for analyzing polymer populations
ATE521716T1 (en) * 2003-03-31 2011-09-15 Univ Mcmaster APTAMER SELECTION METHODS
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
WO2005012548A2 (en) * 2003-08-01 2005-02-10 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
WO2005036171A1 (en) * 2003-10-09 2005-04-21 Koninklijke Philips Electronics N.V. Method and system for detection of a target analyte
US20050112671A1 (en) * 2003-11-07 2005-05-26 U.S. Genomics, Inc. FRET efficiency methods
US20050112585A1 (en) * 2003-11-21 2005-05-26 Dominic Zichi Method for adjusting the quantification range of individual analytes in a multiplexed assay
EP1692486B1 (en) * 2003-12-12 2015-10-28 Saint Louis University Biosensors for detecting macromolecules and other analytes
US7977048B2 (en) 2004-01-13 2011-07-12 Pathogenetix, Inc. Detection and quantification of analytes in solution using polymers
EP1725587A2 (en) * 2004-03-19 2006-11-29 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
US20060003339A1 (en) * 2004-06-30 2006-01-05 Fuernkranz Hans A Two-hybrid system
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US8945840B2 (en) * 2005-06-10 2015-02-03 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
WO2007056250A2 (en) * 2005-11-04 2007-05-18 U.S. Genomics, Inc. Heterogeneous assay of analytes in solution using polymers
EP2613147B1 (en) * 2006-01-17 2015-03-11 Somalogic, Inc. Detection methods using aptamers
WO2007095279A2 (en) * 2006-02-14 2007-08-23 University Of Florida Research Foundation, Inc. Dual nanoparticle assay for detection and separation of biological species
US20110136099A1 (en) * 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
WO2008134069A2 (en) * 2007-04-26 2008-11-06 University Of Florida Research Foundation Anchored ligand agents
US20100294665A1 (en) * 2007-07-12 2010-11-25 Richard Allen Method and system for transferring and/or concentrating a sample
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
SE533515C2 (en) * 2008-04-16 2010-10-12 Aamic Ab Analysis procedure for simultaneous analysis of several analytes
EP2703816B1 (en) 2008-11-21 2016-10-05 Saint Louis University Biosensor for detecting multiple epitopes on a target
US9075053B2 (en) * 2009-02-16 2015-07-07 Dongguk University Industry-Academic Cooperation Foundation Target substance detection method using aptamer
US9329178B2 (en) 2009-02-16 2016-05-03 Dongguk University Industry-Academic Cooperation Foundation Target substance detection method using aptamer
CA2787483C (en) 2010-02-12 2018-03-06 Saint Louis University Molecular biosensors capable of signal amplification
EP2591107A2 (en) 2010-07-06 2013-05-15 Aptateck Bio Ltd. Nucleic acid aptamer-based diagnostic methods with novel techniques for signal enhancement
US20150031585A1 (en) * 2011-03-10 2015-01-29 Somalogic, Inc. Multiaptamer Target Detection
US20130183658A1 (en) * 2012-01-16 2013-07-18 Bret T. Barnhizer Methods and Devices for Rapid Detection of Live Microorganisms by Aptamers and/or Antibodies Immobilized on Permeable Membranes
SG11201602063UA (en) * 2013-09-24 2016-04-28 Somalogic Inc Multiaptamer target detection
US10274484B2 (en) 2014-09-12 2019-04-30 Mediomics Llc Molecular biosensors with a modular design
JP7241619B2 (en) * 2019-06-19 2023-03-17 アークレイ株式会社 Target substance detection method, target substance detection kit, target substance detection system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US51974A (en) * 1866-01-09 Improvement in trusses
DE229046T1 (en) * 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet METHOD FOR OBTAINING DNA, RNS, PEPTIDES, POLYPEPTIDES OR PROTEINS BY DNA RECOMBINANT METHOD.
US5853984A (en) * 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5459015A (en) * 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5789163A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US6449562B1 (en) * 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US6180348B1 (en) * 1998-04-20 2001-01-30 Weihua Li Method of isolating target specific oligonucleotide ligands

Also Published As

Publication number Publication date
US20020037506A1 (en) 2002-03-28
WO2001079562A1 (en) 2001-10-25

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