AU2001244140A1 - Method for removing endotoxins - Google Patents

Method for removing endotoxins

Info

Publication number
AU2001244140A1
AU2001244140A1 AU2001244140A AU4414001A AU2001244140A1 AU 2001244140 A1 AU2001244140 A1 AU 2001244140A1 AU 2001244140 A AU2001244140 A AU 2001244140A AU 4414001 A AU4414001 A AU 4414001A AU 2001244140 A1 AU2001244140 A1 AU 2001244140A1
Authority
AU
Australia
Prior art keywords
removing endotoxins
endotoxins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001244140A
Inventor
Robertus Hendriks
Andrea Lantos
Maria Wehsling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of AU2001244140A1 publication Critical patent/AU2001244140A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AU2001244140A 2000-03-06 2001-02-14 Method for removing endotoxins Abandoned AU2001244140A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10010342A DE10010342A1 (en) 2000-03-06 2000-03-06 Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources
DE10010342 2000-03-06
PCT/EP2001/001610 WO2001066718A1 (en) 2000-03-06 2001-02-14 Method for removing endotoxins

Publications (1)

Publication Number Publication Date
AU2001244140A1 true AU2001244140A1 (en) 2001-09-17

Family

ID=7633346

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001244140A Abandoned AU2001244140A1 (en) 2000-03-06 2001-02-14 Method for removing endotoxins

Country Status (6)

Country Link
US (1) US6617443B2 (en)
EP (1) EP1261702B1 (en)
JP (1) JP2003525630A (en)
AU (1) AU2001244140A1 (en)
DE (2) DE10010342A1 (en)
WO (1) WO2001066718A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040083493A1 (en) * 1997-02-19 2004-04-29 Next Level Communications, Inc. Transmitting caller ID within a digital stream
US20030192053A1 (en) * 1997-02-19 2003-10-09 Next Level Communications, Inc. Method and apparatus for transmitting wireless signals over media
AU2003216830A1 (en) * 2002-03-21 2003-10-08 Avecia Biotechnology Inc. Purification methods for oligonucleotides and their analogs
AU2003250270B2 (en) 2002-06-24 2009-05-07 Hyglos Invest Gmbh Method for identifying and extracting endotoxin
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
FR2848223B1 (en) * 2002-12-09 2005-02-25 Centre Nat Rech Scient NOVEL METHOD OF ISOLATING ENDOTOXINS.
CA2530454A1 (en) * 2003-06-24 2005-01-13 Akzo Nobel N.V. Removal of lipopolysaccharides from protein- lipopolysaccharide complex es by nonflammable solvents
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
DE102005002969A1 (en) 2005-01-21 2006-08-03 Profos Ag Method for detection and removal of endotoxin
EP2409994A3 (en) 2007-02-28 2012-08-15 Lipoxen Technologies Limited Reduction of endotoxin in polysialic acids
US8647857B2 (en) * 2008-04-30 2014-02-11 Gradalis, Inc. Processes for the digestion of colanic acid
US20160215010A1 (en) * 2013-10-03 2016-07-28 Kyowa Hakko Bio Co., Ltd. Method for purifying double-stranded ribonucleic acid
CN108148831A (en) * 2018-01-15 2018-06-12 南京驯鹿医疗技术有限公司 A kind of a large amount of preparation processes of endotoxin-free plasmid
BR112020015253A2 (en) * 2018-03-08 2020-12-08 Catalent U.K. Swindon Zydis Limited PROCESS TO REDUCE ENDOTOXIN IN GELATINE
ES2967202T3 (en) 2021-07-02 2024-04-29 Axagarius Gmbh & Co Kg Branched secondary alcohol ethoxylate mixture for endotoxin removal in anion exchange chromatography
WO2023012206A1 (en) 2021-08-05 2023-02-09 Merck Patent Gmbh Method for reducing endotoxin levels in nucleic acid purification
WO2023170028A1 (en) 2022-03-10 2023-09-14 Merck Patent Gmbh Method for reducing endotoxin levels in nucleic acid purification

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU693511B2 (en) * 1994-02-07 1998-07-02 Qiagen Gmbh Endotoxin reduction or removal process
DE69515028T2 (en) * 1994-03-22 2000-06-29 Immune Response Corp Inc THE HIGHLY EFFICIENT PRODUCTION AND INSULATION OF VIRUS PARTICLES
US6825012B2 (en) * 1995-02-23 2004-11-30 Gencell S.A. DNA molecules, preparation and use in gene therapy
US5837520A (en) * 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification

Also Published As

Publication number Publication date
DE10010342A1 (en) 2001-09-20
WO2001066718A1 (en) 2001-09-13
EP1261702B1 (en) 2007-11-21
US20030068810A1 (en) 2003-04-10
JP2003525630A (en) 2003-09-02
US6617443B2 (en) 2003-09-09
DE50113292D1 (en) 2008-01-03
EP1261702A1 (en) 2002-12-04

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