AU2003216830A1 - Purification methods for oligonucleotides and their analogs - Google Patents

Purification methods for oligonucleotides and their analogs

Info

Publication number
AU2003216830A1
AU2003216830A1 AU2003216830A AU2003216830A AU2003216830A1 AU 2003216830 A1 AU2003216830 A1 AU 2003216830A1 AU 2003216830 A AU2003216830 A AU 2003216830A AU 2003216830 A AU2003216830 A AU 2003216830A AU 2003216830 A1 AU2003216830 A1 AU 2003216830A1
Authority
AU
Australia
Prior art keywords
oligonucleotides
analogs
purification methods
purification
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003216830A
Inventor
Jack T. Johansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
Avecia Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecia Biotechnology Inc filed Critical Avecia Biotechnology Inc
Publication of AU2003216830A1 publication Critical patent/AU2003216830A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2003216830A 2002-03-21 2003-03-19 Purification methods for oligonucleotides and their analogs Abandoned AU2003216830A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36706002P 2002-03-21 2002-03-21
US60/367,060 2002-03-21
PCT/GB2003/001161 WO2003080834A2 (en) 2002-03-21 2003-03-19 Purification methods for oligonucleotides and their analogs

Publications (1)

Publication Number Publication Date
AU2003216830A1 true AU2003216830A1 (en) 2003-10-08

Family

ID=28454833

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003216830A Abandoned AU2003216830A1 (en) 2002-03-21 2003-03-19 Purification methods for oligonucleotides and their analogs

Country Status (7)

Country Link
EP (1) EP1490488A2 (en)
JP (1) JP2005520547A (en)
KR (1) KR20040108672A (en)
CN (1) CN1643145A (en)
AU (1) AU2003216830A1 (en)
CA (1) CA2479901A1 (en)
WO (1) WO2003080834A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006132588A1 (en) * 2005-06-10 2006-12-14 Quiatech Ab Method for the purification of synthetic oligonucleotides containing one or several labels
DE102005059315A1 (en) * 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
AU2012205204B2 (en) * 2005-12-09 2015-03-12 Qiagen Gmbh Method for enriching short-chain nucleic acids
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2445441B (en) 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2443505B (en) 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
DE102008063003A1 (en) * 2008-12-23 2010-06-24 Qiagen Gmbh Nucleic acid purification method
JP5914338B2 (en) * 2009-09-24 2016-05-11 キアジェン ゲイサーズバーグ インコーポレイテッド Compositions, methods and kits for nucleic acid isolation and analysis using anion exchange materials
EP2513335A4 (en) * 2009-12-14 2013-09-11 Betty Wu Method and materials for separating nucleic acid materials
EP2760999A1 (en) * 2011-09-26 2014-08-06 Qiagen GmbH Methods for separating nucleic acids by size
CN103833797A (en) * 2012-11-26 2014-06-04 福建金山生物制药股份有限公司 Industrial method for purifying phosphorothioate oligodeoxynucleotide
WO2014122288A1 (en) 2013-02-08 2014-08-14 Qiagen Gmbh Method for separating dna by size
CN106632558B (en) * 2016-08-25 2019-09-13 淮阴师范学院 A kind of purification process of oligonucleotide and application
JP2022500025A (en) * 2018-09-11 2022-01-04 アムジエン・インコーポレーテツド Purification method for guanine-rich oligonucleotides
AU2020309307B2 (en) * 2019-07-09 2023-03-30 F. Hoffmann-La Roche Ag Process for the deprotection of oligonucleotides
WO2021111418A1 (en) * 2019-12-05 2021-06-10 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
CN111394427B (en) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 Method for extracting trace DNA in soil-containing test material
CN116209757A (en) * 2020-07-14 2023-06-02 沃特世科技公司 Compositions, kits and methods for separating oligonucleotides from matrix components
WO2024089953A1 (en) * 2022-10-27 2024-05-02 住友化学株式会社 Method for producing oligonucleotide

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995027718A2 (en) * 1994-04-08 1995-10-19 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
DE10010342A1 (en) * 2000-03-06 2001-09-20 Merck Patent Gmbh Method for reducing the endotoxin content of nucleic acid (I) is derived from natural, genetic engineering or biotechnological sources is used to produce high-purity plasmid DNA from natural sources

Also Published As

Publication number Publication date
JP2005520547A (en) 2005-07-14
CN1643145A (en) 2005-07-20
KR20040108672A (en) 2004-12-24
CA2479901A1 (en) 2003-10-02
WO2003080834A2 (en) 2003-10-02
WO2003080834A3 (en) 2003-12-31
EP1490488A2 (en) 2004-12-29

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase