ATE96167T1 - Verfahren zur kristallisation von enzymen. - Google Patents
Verfahren zur kristallisation von enzymen.Info
- Publication number
- ATE96167T1 ATE96167T1 AT91902671T AT91902671T ATE96167T1 AT E96167 T1 ATE96167 T1 AT E96167T1 AT 91902671 T AT91902671 T AT 91902671T AT 91902671 T AT91902671 T AT 91902671T AT E96167 T1 ATE96167 T1 AT E96167T1
- Authority
- AT
- Austria
- Prior art keywords
- enzymes
- crystallization
- concentration
- enzyme
- starting material
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/98—Preparation of granular or free-flowing enzyme compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK654089A DK654089D0 (da) | 1989-12-21 | 1989-12-21 | Fremgangsmaade til krystallisation af enzymer og krystallinske enzymer |
DK84890A DK84890D0 (da) | 1990-04-05 | 1990-04-05 | Fremgangsmaade til krystallisation af savinase |
DK84790A DK84790D0 (da) | 1990-04-05 | 1990-04-05 | Fremgangsmaade til krystallisation af enzymer |
PCT/DK1990/000341 WO1991009943A1 (en) | 1989-12-21 | 1990-12-21 | Method for crystallization of enzymes |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE96167T1 true ATE96167T1 (de) | 1993-11-15 |
Family
ID=27220958
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT91902671T ATE96167T1 (de) | 1989-12-21 | 1990-12-21 | Verfahren zur kristallisation von enzymen. |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0506866B1 (de) |
JP (1) | JP2975109B2 (de) |
AT (1) | ATE96167T1 (de) |
DE (1) | DE69004101T2 (de) |
DK (1) | DK0506866T3 (de) |
ES (1) | ES2060360T3 (de) |
WO (1) | WO1991009943A1 (de) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5256557A (en) * | 1991-12-27 | 1993-10-26 | Solvay Enzymes, Inc. | Purified alkaline protease concentrate and method of preparation |
EP0955365A3 (de) * | 1992-06-09 | 2000-12-20 | Chiron Corporation | Kristallisierung von M-CSF |
WO1994004665A1 (de) * | 1992-08-14 | 1994-03-03 | Solvay Enzymes Gmbh & Co. Kg | Neue enzymgranulate |
US5281526A (en) * | 1992-10-20 | 1994-01-25 | Solvay Enzymes, Inc. | Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof |
GB2287713A (en) * | 1994-03-19 | 1995-09-27 | Procter & Gamble | Detergent composition containing pectic enzyme |
US5789362A (en) * | 1994-03-29 | 1998-08-04 | The Procter & Gamble Co. | Detergent composition comprising lipoxidase enzymes |
CN1159208A (zh) * | 1995-07-28 | 1997-09-10 | 吉斯特·布罗卡迪斯股份有限公司 | 盐稳定化酶制剂 |
US6190898B1 (en) * | 1995-10-23 | 2001-02-20 | Genencor International, Inc. | Crystalline cellulase and method for producing same |
US6207437B1 (en) * | 1996-03-11 | 2001-03-27 | Genencor International, Inc. | Crystalline protease and method for producing same |
TW409035B (en) | 1997-06-04 | 2000-10-21 | Gist Brocades Bv | Starch-based enzyme granulates |
WO2000052150A1 (en) | 1999-03-05 | 2000-09-08 | Genencor International, Inc. | Method for rapidly obtaining crystals with desirable morphologies |
CA2433353C (en) * | 2000-12-28 | 2017-03-21 | Altus Biologics, Inc. | Crystals of whole antibodies and fragments thereof and methods for making and using them |
CA2451185A1 (en) | 2001-06-21 | 2003-01-03 | Altus Biologics, Inc. | Spherical protein particles and methods of making and using them |
RU2442571C2 (ru) | 2004-07-23 | 2012-02-20 | Дженентек, Инк. | Кристаллизация антител или их фрагментов |
CN101631797B (zh) | 2007-03-15 | 2015-12-16 | 诺维信公司 | 发酵液中蛋白酶晶体的溶解 |
EP3149178B1 (de) | 2014-05-27 | 2020-07-15 | Novozymes A/S | Lipasevarianten und polynukleotide zur codierung davon |
EP3878957A1 (de) | 2014-05-27 | 2021-09-15 | Novozymes A/S | Verfahren zur herstellung von lipasen |
MX2017007103A (es) | 2014-12-05 | 2017-08-24 | Novozymes As | Variantes de lipasa y polinucleotidos que las codifican. |
CA2987160C (en) | 2015-07-01 | 2022-12-13 | Novozymes A/S | Methods of reducing odor |
WO2017005816A1 (en) | 2015-07-06 | 2017-01-12 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
US10870838B2 (en) | 2015-12-01 | 2020-12-22 | Novozymes A/S | Methods for producing lipases |
EP4357453A2 (de) | 2016-07-18 | 2024-04-24 | Novozymes A/S | Lipasevarianten, polynukleotide zur codierung davon und verwendung davon |
US11078445B2 (en) | 2017-05-05 | 2021-08-03 | Novozymes A/S | Compositions comprising lipase and sulfite |
CN111108183A (zh) | 2017-06-30 | 2020-05-05 | 诺维信公司 | 酶浆液组合物 |
CN111670248A (zh) | 2017-12-04 | 2020-09-15 | 诺维信公司 | 脂肪酶变体以及编码其的多核苷酸 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL33327A (en) * | 1968-11-29 | 1972-12-29 | Lilly Co Eli | A crystalline combination of l-asparaginase and a metal or ammonium or hydrazinium ion and method for preparing the same |
DK8502857A (de) * | 1984-06-25 | 1985-12-26 | ||
JP2886921B2 (ja) * | 1988-03-18 | 1999-04-26 | ジェネンカー インコーポレイテッド | スブチリシンの結晶化法 |
-
1990
- 1990-12-21 EP EP91902671A patent/EP0506866B1/de not_active Expired - Lifetime
- 1990-12-21 DE DE91902671T patent/DE69004101T2/de not_active Expired - Lifetime
- 1990-12-21 WO PCT/DK1990/000341 patent/WO1991009943A1/en active IP Right Grant
- 1990-12-21 ES ES91902671T patent/ES2060360T3/es not_active Expired - Lifetime
- 1990-12-21 JP JP3502525A patent/JP2975109B2/ja not_active Expired - Lifetime
- 1990-12-21 DK DK91902671.6T patent/DK0506866T3/da active
- 1990-12-21 AT AT91902671T patent/ATE96167T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
JP2975109B2 (ja) | 1999-11-10 |
DE69004101D1 (de) | 1993-11-25 |
JPH05502791A (ja) | 1993-05-20 |
WO1991009943A1 (en) | 1991-07-11 |
DE69004101T2 (de) | 1994-03-24 |
DK0506866T3 (da) | 1994-03-21 |
EP0506866A1 (de) | 1992-10-07 |
EP0506866B1 (de) | 1993-10-20 |
ES2060360T3 (es) | 1994-11-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification | ||
REN | Ceased due to non-payment of the annual fee |