ATE433485T1 - Chemisch modifizierte mutante serinhydrolasen - Google Patents

Chemisch modifizierte mutante serinhydrolasen

Info

Publication number
ATE433485T1
ATE433485T1 AT99964973T AT99964973T ATE433485T1 AT E433485 T1 ATE433485 T1 AT E433485T1 AT 99964973 T AT99964973 T AT 99964973T AT 99964973 T AT99964973 T AT 99964973T AT E433485 T1 ATE433485 T1 AT E433485T1
Authority
AT
Austria
Prior art keywords
cysteine
serine hydrolases
chemically modified
group
modified
Prior art date
Application number
AT99964973T
Other languages
English (en)
Inventor
John Jones
Michael Dickman
Original Assignee
Univ Toronto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Toronto filed Critical Univ Toronto
Application granted granted Critical
Publication of ATE433485T1 publication Critical patent/ATE433485T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99964973T 1998-11-10 1999-11-09 Chemisch modifizierte mutante serinhydrolasen ATE433485T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10775898P 1998-11-10 1998-11-10
US11306198P 1998-12-21 1998-12-21
PCT/US1999/026586 WO2000028007A2 (en) 1998-11-10 1999-11-09 Chemically modified mutant serine hydrolases

Publications (1)

Publication Number Publication Date
ATE433485T1 true ATE433485T1 (de) 2009-06-15

Family

ID=26805118

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99964973T ATE433485T1 (de) 1998-11-10 1999-11-09 Chemisch modifizierte mutante serinhydrolasen

Country Status (9)

Country Link
US (4) US20050282248A1 (de)
EP (1) EP1129180B1 (de)
JP (1) JP4932990B2 (de)
AT (1) ATE433485T1 (de)
AU (1) AU772427B2 (de)
CA (1) CA2348014A1 (de)
DE (1) DE69940978D1 (de)
DK (1) DK1129180T3 (de)
WO (1) WO2000028007A2 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
JP2002507426A (ja) 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
ATE433485T1 (de) 1998-11-10 2009-06-15 Univ Toronto Chemisch modifizierte mutante serinhydrolasen
MXPA01010896A (es) * 1999-04-28 2003-06-24 Genencor Int Antagonistas cataliticos especificamente dirigidos al objetivo y usos de los mismos.
CA2376045A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
KR20020021396A (ko) * 1999-07-22 2002-03-20 데이비드 엠 모이어 입체적으로 보호된 에피토프 영역을 갖는 프로테아제콘쥬게이트
BR0012694A (pt) 1999-07-22 2002-04-09 Procter & Gamble Conjugado de protease, composição de limpeza e composição de tratamento pessoal
BR0012693A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante, de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
US7202070B2 (en) 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
EP1359225B1 (de) * 2002-04-08 2006-11-15 Degussa AG Verfahren zur Bestimmung der Syntheseaktivität von Hydrolasen
EP1354960A1 (de) * 2002-04-08 2003-10-22 Degussa AG Verfahren zur Bestimmung der Syntheseaktivität von Hydrolasen
WO2016198143A1 (en) 2015-06-10 2016-12-15 Merck Patent Gmbh Chiral dopants having a nortricyclan unit

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5244791A (en) * 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5116741A (en) * 1988-04-12 1992-05-26 Genex Corporation Biosynthetic uses of thermostable proteases
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
JP3362847B2 (ja) * 1990-08-09 2003-01-07 ジェネンテック インコーポレイテッド ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5837516A (en) 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6946280B1 (en) 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
DK0942962T3 (da) * 1996-11-26 2007-05-07 Genencor Int Kemisk modificerede enzymer
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
KR20010040397A (ko) * 1998-01-23 2001-05-15 마가렛 에이.혼 개질된 효소 및 펩티드 합성에 이들을 사용하는 용도
AU2330899A (en) * 1998-01-23 1999-08-09 Genencor International, Inc. Chemically modified mutant enzymes and methods for producing them and screening them
ATE407941T1 (de) * 1998-07-02 2008-09-15 Univ Toronto Mit einer kohlenhydratgruppe chemisch modifizierte proteine
ATE433485T1 (de) 1998-11-10 2009-06-15 Univ Toronto Chemisch modifizierte mutante serinhydrolasen
JP2002533087A (ja) 1998-12-21 2002-10-08 ジェネンカー インターナショナル，インコーポレイティド 複数荷電変種を有する化学修飾酵素

Also Published As

Publication number Publication date
US20120295329A1 (en) 2012-11-22
US8148128B2 (en) 2012-04-03
EP1129180B1 (de) 2009-06-10
EP1129180A2 (de) 2001-09-05
JP4932990B2 (ja) 2012-05-16
DE69940978D1 (de) 2009-07-23
DK1129180T3 (da) 2009-08-24
AU772427B2 (en) 2004-04-29
CA2348014A1 (en) 2000-05-18
US20120156721A1 (en) 2012-06-21
JP2002529078A (ja) 2002-09-10
US8357524B2 (en) 2013-01-22
WO2000028007A3 (en) 2000-07-27
US20090075329A1 (en) 2009-03-19
WO2000028007A2 (en) 2000-05-18
AU3099000A (en) 2000-05-29
US20050282248A1 (en) 2005-12-22

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