ATE414792T1 - Helicase-abhungige amplifikation von nukleinsuren - Google Patents

Helicase-abhungige amplifikation von nukleinsuren

Info

Publication number
ATE414792T1
ATE414792T1 AT03754618T AT03754618T ATE414792T1 AT E414792 T1 ATE414792 T1 AT E414792T1 AT 03754618 T AT03754618 T AT 03754618T AT 03754618 T AT03754618 T AT 03754618T AT E414792 T1 ATE414792 T1 AT E414792T1
Authority
AT
Austria
Prior art keywords
nucleic acids
helicase
dependent amplification
amplification
dependent
Prior art date
Application number
AT03754618T
Other languages
English (en)
Inventor
Huimin Kong
Myriam Vincent
Yan Xu
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32033597&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE414792(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Application granted granted Critical
Publication of ATE414792T1 publication Critical patent/ATE414792T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Optical Recording Or Reproduction (AREA)
  • Glass Compositions (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
AT03754618T 2002-09-20 2003-09-19 Helicase-abhungige amplifikation von nukleinsuren ATE414792T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41229802P 2002-09-20 2002-09-20
US44666203P 2003-02-11 2003-02-11

Publications (1)

Publication Number Publication Date
ATE414792T1 true ATE414792T1 (de) 2008-12-15

Family

ID=32033597

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03754618T ATE414792T1 (de) 2002-09-20 2003-09-19 Helicase-abhungige amplifikation von nukleinsuren

Country Status (8)

Country Link
US (3) US7282328B2 (de)
EP (1) EP1539979B1 (de)
JP (1) JP4632788B2 (de)
AT (1) ATE414792T1 (de)
AU (1) AU2003272438B2 (de)
CA (1) CA2498764C (de)
DE (1) DE60324810D1 (de)
WO (1) WO2004027025A2 (de)

Families Citing this family (270)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
DE60322044D1 (de) * 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
CN101824409B (zh) * 2003-04-25 2015-11-25 贝克顿·迪金森公司 通过核酸扩增检测1型和2型单纯疱疹病毒
US20050069926A1 (en) * 2003-08-01 2005-03-31 Affymetrix, Inc. Helicase-amplified reverse transcription
EP1692486B1 (de) 2003-12-12 2015-10-28 Saint Louis University Biosensoren zum nachweis von makromolekülen und anderen analyten
US7378242B2 (en) * 2004-03-18 2008-05-27 Atom Sciences, Inc. DNA sequence detection by limited primer extension
EP3540073B1 (de) * 2004-06-01 2021-08-25 Abbott Diagnostics Scarborough, Inc. Rekombinase-polymerase-amplifikation
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US7700283B2 (en) * 2004-10-21 2010-04-20 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
JP5165383B2 (ja) 2004-12-23 2013-03-21 アイ−スタツト・コーポレイシヨン 分子診断システム及び方法
EP1833995B1 (de) 2005-01-05 2017-08-02 Biohelix Corporation Identifizierung von rna-targets mittels helikasen
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7824890B2 (en) * 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
US7635594B2 (en) 2005-05-09 2009-12-22 Theranos, Inc. Point-of-care fluidic systems and uses thereof
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (de) 2005-06-10 2012-07-04 Saint Louis University Verfahren zur selektion von aptameren
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
US7435561B2 (en) 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US8703445B2 (en) 2005-12-29 2014-04-22 Abbott Point Of Care Inc. Molecular diagnostics amplification system and methods
WO2007087262A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Selective genome amplification
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US20100173364A1 (en) * 2006-04-11 2010-07-08 New England Biolabs, Inc. Repair of Nucleic Acids for Improved Amplification
WO2007120808A2 (en) * 2006-04-14 2007-10-25 Biohelix Corporation A method of asymmetric helicase-dependent amplification for probe-based detection of targets
EP2029782B1 (de) 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
DK1945821T3 (da) 2006-06-06 2011-03-07 Gen Probe Inc Mærkede oligonukleotider og anvendelse deraf i fremgangsmåder til amplifikation af nukleinsyrer
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
WO2008058018A2 (en) 2006-11-02 2008-05-15 Mayo Foundation For Medical Education And Research Predicting cancer outcome
CN101617046A (zh) 2007-02-23 2009-12-30 新英格兰生物实验室公司 使用体外区室化选择和富集蛋白质
US7838235B2 (en) 2007-03-28 2010-11-23 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
AU2008269201B2 (en) 2007-06-21 2011-08-18 Gen-Probe Incorporated Instrument and receptacles for use in performing processes
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
SG10201606120XA (en) 2007-10-02 2016-09-29 Theranos Inc Modular Point-Of-Care Devices And Uses Thereof
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
EP3067694A1 (de) 2008-05-05 2016-09-14 Los Alamos National Security, LLC Vorbereitung von nukleinsäureproben auf lateralflussbasis und passive strömungssteuerung
US10316352B2 (en) 2008-05-13 2019-06-11 Gen-Probe Incorporated Methods of capturing a target nucleic acid for amplification and detection using an inactivatable target capture oligomer
CA2725978A1 (en) 2008-05-28 2009-12-03 Genomedx Biosciences, Inc. Systems and methods for expression-based discrimination of distinct clinical disease states in prostate cancer
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
CA2744003C (en) 2008-11-21 2016-03-15 Mediomics Llc Biosensor for detecting multiple epitopes on a target
WO2010088273A1 (en) 2009-01-27 2010-08-05 Qiagen Gaithersburg Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
JP2010172248A (ja) * 2009-01-29 2010-08-12 National Institute Of Advanced Industrial Science & Technology 核酸の新規定量方法並びにそれに用いる新規試薬キット
JP5586631B2 (ja) 2009-01-30 2014-09-10 ジェン−プローブ・インコーポレーテッド 信号を検出し、信号伝送要素に熱エネルギーを加えるためのシステム及び方法
US8685648B2 (en) 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
WO2010123626A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms and expression predict clinical outcome in patients with cancer
WO2010129934A2 (en) 2009-05-07 2010-11-11 Veracyte, Inc. Methods and compositions for diagnosis of thyroid conditions
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP3663750B1 (de) 2009-05-29 2021-11-03 Life Technologies Corporation Nukleinsäuregerüstpolymerpartikel und verfahren zur herstellung und verwendung
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2456887B1 (de) 2009-07-21 2015-11-25 Gen-Probe Incorporated Verfahren und zusammensetzungen für den quantitativen nachweis von nukleinsäuresequenzen in einem erweiterten dynamischen bereich
CA2770888C (en) 2009-08-31 2020-04-14 Gen-Probe Incorporated Dengue virus assay
WO2011047223A1 (en) 2009-10-16 2011-04-21 Monsanto Technology Llc Methods of polynucleotide detection
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
WO2011084757A1 (en) 2009-12-21 2011-07-14 University Of Southern California Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
JP2013516192A (ja) * 2010-01-08 2013-05-13 キアジェン ゲイサーズバーグ インコーポレイテッド 等温核酸増幅のための材料および方法
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
CA2787483C (en) 2010-02-12 2018-03-06 Saint Louis University Molecular biosensors capable of signal amplification
US20110262903A1 (en) 2010-02-26 2011-10-27 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
PL2556171T3 (pl) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Oznaczenia biologiczne kodowane przestrzennie
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2011150227A1 (en) 2010-05-26 2011-12-01 Qiagen Gaithersburg, Inc. Quantitative helicase assay
EP2588629B1 (de) 2010-06-30 2017-05-17 Gen-Probe Incorporated Methode und apparatur zur identifizierung von analyt-beinhaltenden proben unter verwendung von single-read determination des analyten und prozesskontrollsignalen
WO2012006542A2 (en) * 2010-07-08 2012-01-12 University Of Central Florida Research Foundation, Inc. Method of detecting single nucleotide polymorphisms
EP2420579A1 (de) 2010-08-17 2012-02-22 QIAGEN GmbH Helikasenabhängige isothermale Amplifikation mit Nicking-Enzymen
WO2012051199A2 (en) 2010-10-12 2012-04-19 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event mon87712 and methods for detection thereof
SG192069A1 (en) 2011-01-21 2013-08-30 Theranos Inc Systems and methods for sample use maximization
CA2831140C (en) 2011-04-07 2017-11-07 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR101508670B1 (ko) 2011-04-20 2015-04-07 메사 테크 인터내셔널, 인코포레이티드 핵산 검출 및 동정을 위한 통합 장치
CA2840630C (en) 2011-06-30 2021-11-30 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof
WO2013033528A1 (en) 2011-09-01 2013-03-07 Jennifer Ong Compositions and methods relating to variant dna polymerases and synthetic dna polymerases
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8380541B1 (en) 2011-09-25 2013-02-19 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
EP2758547B1 (de) 2011-09-21 2015-09-16 Gen-Probe Incorporated Verfahren zur amplifikation von nukleinsäuren unter verwendung von tag-vermittelter verschiebung
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
MX344792B (es) 2011-09-25 2017-01-06 Theranos Inc Sistemas y métodos para múltiples análisis.
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
EP2791359B1 (de) 2011-12-13 2020-01-15 Decipher Biosciences, Inc. Krebsdiagnostik mit nichtcodierenden transkripten
US10385382B2 (en) 2011-12-29 2019-08-20 Oxford Nanopore Technologies Ltd. Enzyme method
JP6466173B2 (ja) * 2012-02-14 2019-02-06 ヴェラ オペレーションズ ユーエスエー，インコーポレーテッド ブロック型プライマーを用いた等温増幅法
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
UA116205C2 (uk) 2012-04-09 2018-02-26 Енвіролоджикс Інк. Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку
ES2823551T3 (es) 2012-06-08 2021-05-07 Ionian Tech Llc Amplificaciones de ácido nucleico
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
CA2879355C (en) * 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
CA2879261C (en) 2012-07-19 2022-12-06 Oxford Nanopore Technologies Limited Modified helicases
AU2013202793B2 (en) 2012-07-31 2014-09-18 Gen-Probe Incorporated System, method and apparatus for automated incubation
US11035005B2 (en) 2012-08-16 2021-06-15 Decipher Biosciences, Inc. Cancer diagnostics using biomarkers
US8993298B1 (en) 2012-08-31 2015-03-31 New England Biolabs, Inc. DNA polymerases
BR112015005429A2 (pt) 2012-09-11 2017-07-04 Theranos Inc sistemas de gestão de informação e métodos usando uma assinatura biológica
CN102912038B (zh) * 2012-10-25 2014-05-28 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的rt-hda试剂盒及引物
CN102876813B (zh) * 2012-10-25 2013-11-13 中华人民共和国北京出入境检验检疫局 一种检测禽流感病毒的实时荧光rt-hda试剂盒及引物
US9074248B1 (en) 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
EP2935622A1 (de) * 2012-12-18 2015-10-28 Qiagen GmbH Verfahren zur amplifikation von nukleinsäuren
MX354033B (es) 2013-02-18 2018-02-09 Theranos Ip Co Llc Sistemas y métodos para recolectar y transmitir resultados de ensayos.
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
EP2971106B1 (de) 2013-03-11 2020-02-26 ELITechGroup, Inc. Verfahren für echte isothermale strangverschiebungsamplifikation
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
EP2861996B1 (de) 2013-03-11 2019-03-06 Cue Health Inc. Probenanalyse-kartusche
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
CN118240918A (zh) 2013-06-25 2024-06-25 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
US9920358B2 (en) 2013-06-27 2018-03-20 New England Biolabs, Inc. Helicase suppression of non-template amplification
CN105473737B (zh) 2013-07-25 2019-10-25 德诚分子诊断 用于检测细菌污染的方法和组合物
US9315842B2 (en) * 2013-08-19 2016-04-19 Steven A Benner Helicase dependent amplification of DNA molecules using nucleotide analogs
EP3502127B1 (de) 2013-10-09 2023-07-26 Monsanto Technology LLC Transgenes ereignis mon87403 in mais und nachweisverfahren dafür
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN105899678A (zh) 2013-10-18 2016-08-24 牛津纳米孔技术公司 经修饰的酶
EP3063272A2 (de) 2013-10-30 2016-09-07 Green Life Biotech, LLC Pathogenesequantifizierungssysteme und behandlungsverfahren für mehltau von zitrusgewächsen
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CA2933343A1 (en) 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants
RU2723079C2 (ru) 2014-04-22 2020-06-08 Инвайролоджикс, Инк. Композиции и способы для увеличения и/или прогнозирования амплификации днк
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
EP3169793B1 (de) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermische verfahren zur amplifizierung von nukleinsäureproben
WO2016013620A1 (ja) * 2014-07-24 2016-01-28 学校法人関西学院 好熱性アーキア由来ＤＥＡＤ－ｂｏｘ型ＲＮＡへリカーゼ及びその改変体を用いるＤＮＡ増幅方法、並びにその方法のために使用する好熱性アーキア由来ＤＥＡＤ－ｂｏｘ型ＲＮＡへリカーゼ及びその改変体
US10274484B2 (en) 2014-09-12 2019-04-30 Mediomics Llc Molecular biosensors with a modular design
JP6824881B2 (ja) * 2014-10-17 2021-02-03 オックスフォード ナノポール テクノロジーズ リミテッド ナノ細孔ｒｎａを特徴付けるための方法
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
EP3770274A1 (de) 2014-11-05 2021-01-27 Veracyte, Inc. Systeme und verfahren zur diagnose von idiopathischer pulmonaler fibrose auf transbronchialen biopsien mit maschinellem lernen und hochdimensionalen transkriptionsdaten
MY182476A (en) 2014-11-11 2021-01-25 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
WO2016077763A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
EP3882356A1 (de) 2014-12-15 2021-09-22 Illumina, Inc. Zusammensetzungen und verfahren zur einzelmolekülplatzierung auf einem substrat
EP3250715A4 (de) 2015-01-30 2018-10-24 Envirologix Inc. Substratmolekül
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
EP4119677B1 (de) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
EP3283512A4 (de) 2015-04-17 2018-10-03 Distributed Bio Inc Verfahren zur massenhumanisierung von nichthumanen antikörpern
AU2016253147B2 (en) 2015-04-24 2021-07-22 Mesa Biotech, Inc. Fluidic test cassette
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物***公司 利用有限核苷酸组成的引物扩增
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
ES2789349T3 (es) 2015-05-29 2020-10-26 Illumina Cambridge Ltd Utilización mejorada de cebadores de superficie en grupos
EP3689466B1 (de) 2015-07-17 2024-06-05 Cue Health Inc. System zur erweiterten detektion und quantifizierung von analyten
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
MX2017010965A (es) 2015-09-15 2017-10-20 Neogen Corp Metodos de deteccion de listeria a partir de una muestra ambiental.
CN115927547A (zh) 2015-12-03 2023-04-07 安可济控股有限公司 用于形成连接产物的方法和组合物
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN109312396A (zh) 2016-04-07 2019-02-05 伊鲁米那股份有限公司 用于构建标准化核酸文库的方法和***
WO2017201102A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
SG11201810907UA (en) 2016-06-06 2019-01-30 Redvault Biosciences Lp Target reporter constructs and uses thereof
US11535883B2 (en) 2016-07-22 2022-12-27 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
JP6853523B2 (ja) * 2016-07-27 2021-03-31 国立大学法人京都大学 ヘリカーゼを用いたｐｃｒ
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
EP3504348B1 (de) 2016-08-24 2022-12-14 Decipher Biosciences, Inc. Verwendung von genomischen signaturen zur vorhersage des ansprechens von patienten mit prostatakrebs auf postoperative strahlentherapie
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
CN107058287B (zh) * 2017-01-16 2020-06-16 浙江大学 一种恒温扩增体系中生成单链产物的方法
WO2018132916A1 (en) 2017-01-20 2018-07-26 Genomedx Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
WO2018140540A1 (en) 2017-01-25 2018-08-02 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
US11873532B2 (en) 2017-03-09 2024-01-16 Decipher Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
JP6448695B2 (ja) 2017-03-21 2019-01-09 株式会社東芝 Ｒｎａ増幅方法、ｒｎａ検出方法及びアッセイキット
EP3601617B3 (de) 2017-03-24 2024-03-20 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis oder zur quantifizierung des parainfluenzavirus
CA3059839C (en) 2017-04-23 2023-01-03 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
FI3842545T3 (fi) 2017-04-23 2023-01-31 Koostumuksia ja menetelmiä näytteiden tunnistuksen parantamiseksi indeksoiduissa nukleiinihappokirjastoissa
US10975430B2 (en) 2017-04-23 2021-04-13 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3872187B1 (de) 2017-04-23 2022-09-21 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
WO2018205035A1 (en) 2017-05-12 2018-11-15 Genomedx Biosciences, Inc Genetic signatures to predict prostate cancer metastasis and identify tumor agressiveness
ES2959047T3 (es) 2017-06-07 2024-02-19 Univ Oregon Health & Science Bibliotecas de genoma completo de célula individual para la secuenciación de metilación
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
CN111032209A (zh) 2017-07-10 2020-04-17 简·探针公司 分析***和方法
CN110785813A (zh) 2017-07-31 2020-02-11 伊鲁米那股份有限公司 具有多路生物样本聚合的测序***
CA3076516A1 (en) 2017-10-02 2019-04-11 Quidel Corporation Phage-based detection method for antimicrobial susceptibility testing and identification of bacterial species
WO2019118550A1 (en) 2017-12-13 2019-06-20 Gen-Probe Incorporated Methods for biological sample processing
JP2021506274A (ja) 2017-12-15 2021-02-22 ジェン−プローブ・インコーポレーテッド 毒素産生Ｃｌｏｓｔｒｉｄｉｕｍ ｄｉｆｆｉｃｉｌｅを検出するための組成物および方法
AU2019212931A1 (en) 2018-01-29 2020-08-27 Gen-Probe Incorporated Analytical systems and methods
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
CA3064622A1 (en) 2018-04-02 2019-10-10 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CN112941155B (zh) * 2018-05-14 2023-07-14 北京艾克伦医疗科技有限公司 具有茎环结构的dna引物对及其应用
CA3067435C (en) 2018-05-17 2023-09-12 Illumina, Inc. High-throughput single-cell sequencing with reduced amplification bias
SG11202000905PA (en) 2018-06-04 2020-02-27 Illumina Inc High-throughput single-cell transcriptome libraries and methods of making and of using
AU2019286648B2 (en) 2018-06-13 2023-11-23 Gen-Probe Incorporated Compositions and methods for detecting group B Streptococcus nucleic acid
KR20210040943A (ko) * 2018-06-26 2021-04-14 매사추세츠 인스티튜트 오브 테크놀로지 Crispr 이펙터 시스템 기반 증폭 방법, 시스템, 및 진단
EP3820616A1 (de) 2018-07-10 2021-05-19 Gen-Probe Incorporated Verfahren und systeme zur detektion und quantifizierung von nukleinsäuren
WO2020028631A1 (en) 2018-08-01 2020-02-06 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of epstein-barr virus
EP3841223A1 (de) 2018-08-08 2021-06-30 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zum nachweis von mycoplasma genitalium
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3856934A2 (de) 2018-09-27 2021-08-04 Gen-Probe Incorporated Zusammensetzungen und verfahren zur detektion von bordetella-pertussis- und bordetella-parapertussis-nukleinsäure
US20220064630A1 (en) 2018-12-10 2022-03-03 10X Genomics, Inc. Resolving spatial arrays using deconvolution
FI3899037T3 (fi) 2018-12-19 2023-11-21 Illumina Inc Menetelmiä polynukleotidiklusterin klonaalisuusprioriteetin parantamiseksi
WO2020142347A2 (en) 2018-12-31 2020-07-09 Gen-Probe Incorporated Systems and methods for filling multi-well cartridges with solid reagents
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
KR20210134598A (ko) 2019-03-01 2021-11-10 일루미나, 인코포레이티드 고-처리량 단일-핵 및 단일-세포 라이브러리 및 이의 제조 및 사용 방법
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
EP3938538A1 (de) 2019-03-15 2022-01-19 10X Genomics, Inc. Verfahren zur verwendung von räumlichen anordnungen für einzelzellsequenzierung
US20220098647A1 (en) 2019-03-22 2022-03-31 Gen-Probe Incorporated Compositions and Methods for Detecting Group A Streptococcus
EP3887542A1 (de) 2019-03-22 2021-10-06 10X Genomics, Inc. Dreidimensionale räumliche analyse
EP3963338A2 (de) 2019-05-03 2022-03-09 Gen-Probe Incorporated Behältertransportsystem für ein analysesystem
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
WO2021046270A1 (en) 2019-09-05 2021-03-11 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
DE112019007863T5 (de) 2019-10-30 2022-08-11 Mitsubishi Electric Corporation Solenoidvorrichtung und Anlasser
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
US20230002812A1 (en) 2019-11-13 2023-01-05 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2021102003A1 (en) 2019-11-18 2021-05-27 10X Genomics, Inc. Systems and methods for tissue classification
EP4062373A1 (de) 2019-11-21 2022-09-28 10X Genomics, Inc. Räumliche analyse von analyten
US11501440B2 (en) 2019-11-22 2022-11-15 10X Genomics, Inc. Systems and methods for spatial analysis of analytes using fiducial alignment
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
AU2021249046A1 (en) 2020-03-30 2022-10-20 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
BR112022021086A2 (pt) 2020-04-30 2022-12-13 Stab Vida Investig E Servicos Em Ciencias Biologicas Lda Sistema integrado para a detecção e identificação de sequências específicas de ácidos nucleicos e método de utilização do sistema integrado
CA3177286A1 (en) 2020-05-12 2021-11-18 Illumina Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
WO2022006081A1 (en) 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
EP4182482A1 (de) 2020-07-17 2023-05-24 Gen-Probe Incorporated Nachweis von makrolidresistentem mycoplasma genitalium
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022087257A2 (en) 2020-10-21 2022-04-28 Gen-Probe Incorporated Fluid container management system
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
AU2022212112A1 (en) 2021-01-29 2023-08-17 Illumina Cambridge Limited Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
WO2022197634A1 (en) 2021-03-15 2022-09-22 Gen-Probe Incorporated Compositions and methods for biological sample processing
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
US20220396778A1 (en) * 2021-06-12 2022-12-15 Shi-Lung Lin Novel RNA Composition and Production Method for Use in iPS Cell Generation
EP4382621A2 (de) 2021-07-27 2024-06-12 Gen-Probe Incorporated Zusammensetzungen und verfahren zum nachweis von c. coli und mindestens einer von salmonella, c. jejuni, shigella und stec
EP4381094A1 (de) 2021-08-03 2024-06-12 Wageningen Universiteit Argonautenbasiertes nukleinsäurenachweissystem
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
US20230279462A1 (en) * 2022-03-01 2023-09-07 Spectacular Labs, Inc. Methods for detecting microorganisms
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
EP4282980A1 (de) 2022-05-23 2023-11-29 Mobidiag Oy Methoden zum verstärken einer nukleinsäure
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5541099A (en) 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH05146299A (ja) 1991-11-27 1993-06-15 Toyobo Co Ltd 核酸配列の増幅方法およびそのための試薬キツト
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
JPH0716094A (ja) * 1993-06-15 1995-01-20 Toyobo Co Ltd Ｒｎａポリメラーゼのプロモーター配列をもつ１本鎖オリゴヌクレオチドを製造する方法
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1999037820A1 (en) 1998-01-26 1999-07-29 Saigene Corporation An exponential nucleic acid amplification method using a single primer of opposing polarity
US6218122B1 (en) 1998-06-19 2001-04-17 Rosetta Inpharmatics, Inc. Methods of monitoring disease states and therapies using gene expression profiles
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
CA2375769A1 (en) * 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
PT1198585E (pt) 1999-06-28 2007-09-20 Source Precision Medicine Inc Processo para caracterizar uma condição biológica usando perfis calibrados da expressão de genes
AU6385800A (en) * 1999-07-30 2001-02-19 Stratagene Archaeal replication accessory factors and methods of use
WO2002002740A2 (en) 2000-07-05 2002-01-10 Rosetta Inpharmatics, Inc. Methods and compositions for determining gene function
ES2305289T3 (es) * 2001-04-20 2008-11-01 The Penn State Research Foundation Metodo de manipulacion de acidos nucleicos.
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
DE60322044D1 (de) * 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation

Also Published As

Publication number Publication date
US20040058378A1 (en) 2004-03-25
DE60324810D1 (de) 2009-01-02
WO2004027025A2 (en) 2004-04-01
JP2006500028A (ja) 2006-01-05
EP1539979B1 (de) 2008-11-19
CA2498764A1 (en) 2004-04-01
US20070254304A1 (en) 2007-11-01
CA2498764C (en) 2015-11-10
AU2003272438B2 (en) 2009-04-02
US7829284B2 (en) 2010-11-09
AU2003272438A1 (en) 2004-04-08
EP1539979A2 (de) 2005-06-15
EP1539979A4 (de) 2006-01-11
US20070207495A1 (en) 2007-09-06
WO2004027025A3 (en) 2004-07-08
JP4632788B2 (ja) 2011-02-16
US7282328B2 (en) 2007-10-16

Similar Documents

Publication Publication Date Title
ATE414792T1 (de) Helicase-abhungige amplifikation von nukleinsuren
HUP0302803A2 (hu) Nukleinsavak izotermikus amplifikációja szilárd hordozón
ATE329059T1 (de) Eine vollständig intern kontrollierte amplifikationsreaktion ermöglichende zusammensetzungen und verfahren
ATE437945T1 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
WO2004048931A3 (en) Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
DE602004029323D1 (de) Verfahren und System zum Auswählen und Manipulieren von mehreren Objekten
WO2002101022A3 (en) Method for rapid amplification of dna
WO2004065582A3 (en) Amplification of dna in a hairpin structure, and applications
BRPI0413949A (pt) aparelho e sistema para a manipulação de entidades celulares
ATE323177T1 (de) Nachweis von pilzpathogenen durch anwendung der polymerase kettenreaktion
EP0702090A3 (de) Verfahren zur Koamplifikation einer Zielnukleinsäure unter Versendung eines Volumenabscheidungsmittel in der Reaktionszusammensetzung, Testsatz und Testvorrichtung
ATE466080T1 (de) Formulierungen und verfahren zum denaturieren von proteinen
ATE401421T1 (de) Verfahren zum nachweis von dna methylierung
WO2006030455A8 (en) Dna polymerases having strand displacement activity
DE60332382D1 (de) Zentrifugalvorrichtung und verfahren zur durchführung von bindungsversuchen
DE60139544D1 (de) Veränderte, fluoreszierende proteine
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
DE60332378D1 (de) Polynukleotide zur amplifikation undzum nachweis von chlamydia trachomatis und neisseria gonorrhoeae
BRPI0001536B8 (de)
AU2002367750A8 (en) Isothermal amplification in nucleic acid analysis
DE60236996D1 (de) Kulturmedium zum nachweis von bakterien der gattung listeria
DE60324819D1 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
ATE443261T1 (de) Scintillation proximity assay

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties