ATE346156T1 - Umkehrtransfektionsverfahren - Google Patents

Umkehrtransfektionsverfahren

Info

Publication number
ATE346156T1
ATE346156T1 AT00963550T AT00963550T ATE346156T1 AT E346156 T1 ATE346156 T1 AT E346156T1 AT 00963550 T AT00963550 T AT 00963550T AT 00963550 T AT00963550 T AT 00963550T AT E346156 T1 ATE346156 T1 AT E346156T1
Authority
AT
Austria
Prior art keywords
reverse transfection
transfection process
reverse
cells
arrays
Prior art date
Application number
AT00963550T
Other languages
English (en)
Inventor
David M Sabatini
Original Assignee
Whitehead Biomedical Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Biomedical Inst filed Critical Whitehead Biomedical Inst
Application granted granted Critical
Publication of ATE346156T1 publication Critical patent/ATE346156T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Laminated Bodies (AREA)
  • Wrappers (AREA)
AT00963550T 1999-09-17 2000-09-18 Umkehrtransfektionsverfahren ATE346156T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15473799P 1999-09-17 1999-09-17
US19358000P 2000-03-30 2000-03-30

Publications (1)

Publication Number Publication Date
ATE346156T1 true ATE346156T1 (de) 2006-12-15

Family

ID=26851730

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00963550T ATE346156T1 (de) 1999-09-17 2000-09-18 Umkehrtransfektionsverfahren

Country Status (7)

Country Link
US (1) US6544790B1 (de)
EP (1) EP1218529B1 (de)
JP (1) JP2003509060A (de)
AT (1) ATE346156T1 (de)
CA (1) CA2383423A1 (de)
DE (1) DE60031997D1 (de)
WO (1) WO2001020015A1 (de)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6515009B1 (en) 1991-09-27 2003-02-04 Neorx Corporation Therapeutic inhibitor of vascular smooth muscle cells
US5811447A (en) 1993-01-28 1998-09-22 Neorx Corporation Therapeutic inhibitor of vascular smooth muscle cells
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
IL135776A0 (en) * 1997-10-24 2001-05-20 Life Technologies Inc Recombinational cloning using nucleic acids having recombination sites
US20050221280A1 (en) 1998-02-02 2005-10-06 Odyssey Thera, Inc. Protein-protein interactions for pharmacological profiling
US6990221B2 (en) * 1998-02-07 2006-01-24 Biodiscovery, Inc. Automated DNA array image segmentation and analysis
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
US6731781B1 (en) * 1999-09-30 2004-05-04 Biodiscovery, Inc. System and method for automatically processing microarrays
US7099502B2 (en) * 1999-10-12 2006-08-29 Biodiscovery, Inc. System and method for automatically processing microarrays
NZ539430A (en) * 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
US8143195B2 (en) * 2000-01-24 2012-03-27 Yingjian Wang Arrays for bringing two or more reagents in contact with one or more biological targets and methods for making and using the arrays
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU2001284373A1 (en) * 2000-08-29 2002-03-13 Yeda Research And Development Co. Ltd. Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents
US20050158702A1 (en) * 2000-09-05 2005-07-21 Stuelpnagel John R. Cellular arrays comprising encoded cells
US6897067B2 (en) 2000-11-03 2005-05-24 Regents Of The University Of Michigan Surface transfection and expression procedure
US6902933B2 (en) 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
JP2005505231A (ja) * 2000-11-03 2005-02-24 リージェンツ オブ ザ ユニバーシティ オブ ミシガン 表面トランスフェクションおよび発現法
US6890556B1 (en) * 2001-02-14 2005-05-10 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
US7029697B2 (en) * 2001-02-14 2006-04-18 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
US20030096414A1 (en) * 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
US20070111201A1 (en) * 2001-04-30 2007-05-17 Benjamin Doranz Reverse transfection of cell arrays for structural and functional analyses of proteins
WO2002090927A2 (en) * 2001-05-04 2002-11-14 Axiom Biotechnologies, Inc. Matrix assays in genomically indexed cells
JP2004532636A (ja) * 2001-05-21 2004-10-28 インヴィトロジェン コーポレーション 核酸分子の単離に用いるための組成物および方法
US6670129B2 (en) 2001-09-24 2003-12-30 Corning Incorporated Cell transfection apparatus and methods for making and using the cell transfection apparatus
US20030124549A1 (en) * 2001-10-11 2003-07-03 Xerox Corporation Devices and methods for detecting genetic sequences
AU2003213791A1 (en) * 2002-03-05 2003-09-22 Vitra Bioscience, Inc. Multiplexed cell transfection using coded carriers
US20070054266A1 (en) * 2002-05-28 2007-03-08 National Institute Of Advanced Industrial Science And Technology Chemical sensor system
JP2005528102A (ja) * 2002-06-03 2005-09-22 パムジーン ビー.ブイ. 多用途の生存マイクロアレイを用いる、細胞に基づく高処理量アッセイのための方法
US7105347B2 (en) 2002-07-30 2006-09-12 Corning Incorporated Method and device for protein delivery into cells
JP3887588B2 (ja) * 2002-08-30 2007-02-28 株式会社リガク X線回折による応力測定法
US7512496B2 (en) * 2002-09-25 2009-03-31 Soheil Shams Apparatus, method, and computer program product for determining confidence measures and combined confidence measures for assessing the quality of microarrays
US7422853B1 (en) * 2002-10-04 2008-09-09 Myriad Genetics, Inc. RNA interference using a universal target
US6905878B2 (en) * 2002-12-19 2005-06-14 The Scripps Research Institute DNA array for high throughput solid-phase transfection and method for producing the same
US20070269891A9 (en) * 2003-01-13 2007-11-22 Yasunobu Tanaka Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
US7691580B2 (en) 2003-01-29 2010-04-06 Corning Incorporated Reverse protein delivery into cells on coded microparticles
EP1644538A4 (de) * 2003-06-26 2006-11-08 Invitrogen Corp Verfahren und zusammensetzungen zum nachweis von promotoraktivität und zur expression von fusionsproteinen
US7883720B2 (en) * 2003-07-09 2011-02-08 Wisconsin Alumni Research Foundation Charge-dynamic polymers and delivery of anionic compounds
WO2005012548A2 (en) * 2003-08-01 2005-02-10 Dynal Biotech Inc. Self-hybridizing multiple target nucleic acid probes and methods of use
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US7553631B2 (en) * 2003-12-11 2009-06-30 Beth Israel Deaconess Medical Center, Inc. RBP4 in insulin sensitivity/resistance, diabetes, and obesity
US20090280567A1 (en) * 2004-02-06 2009-11-12 Dharmacon, Inc. Stabilized sirnas as transfection controls and silencing reagents
EP2123759B1 (de) * 2004-02-06 2014-01-15 Thermo Fisher Scientific Biosciences Inc. Stabilisierte RNA als Transfektionskontrollen und Dämpfungsreagenzien
US7125709B2 (en) * 2004-02-10 2006-10-24 Nitto Denko Corporation Culture device and method for eukaryotic cell transfection
WO2005083091A1 (en) * 2004-02-18 2005-09-09 Nitto Denko Corporation Culture device and method for eukaryotic cell transfection
KR101147147B1 (ko) * 2004-04-01 2012-05-25 머크 샤프 앤드 돔 코포레이션 Rna 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
GB0410105D0 (en) * 2004-05-06 2004-06-09 Amersham Biosciences Uk Ltd Method for characterising compounds
US7935811B2 (en) * 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US7923207B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Apparatus and system having dry gene silencing pools
US7923206B2 (en) * 2004-11-22 2011-04-12 Dharmacon, Inc. Method of determining a cellular response to a biological agent
EP1814994A4 (de) * 2004-11-22 2010-07-14 Dharmacon Inc Vorrichtung und system mit trockenen kontrollgen-verstummungszusammensetzungen
US20060160169A1 (en) * 2004-12-03 2006-07-20 Board Of Regents, The University Of Texas System Cell microarray for profiling of cellular phenotypes and gene function
JP4739352B2 (ja) * 2004-12-17 2011-08-03 日東電工株式会社 真核細胞をトランスフェクトするための固定化分解性カチオンポリマーを持つ固体表面
DE602005015028D1 (de) * 2005-02-03 2009-07-30 King Faisal Specialist Hospita Verfahren zur herstellung transkriptionsaktiver linearer dna-moleküle und deren verwendung auf arrays
US20060223777A1 (en) * 2005-03-29 2006-10-05 Dharmacon, Inc. Highly functional short hairpin RNA
US20060257858A1 (en) * 2005-04-01 2006-11-16 Invitrogen Corporation Methods and compositions for introducing biopolymers into cells
EP2631652A1 (de) 2005-04-05 2013-08-28 Corning Incorporated Markierungsfreie Biosensoren
US8734851B2 (en) * 2005-04-29 2014-05-27 Wisconsin Alumni Research Foundation Localized delivery of nucleic acid by polyelectrolyte assemblies
JP5105600B2 (ja) * 2005-05-17 2012-12-26 国立大学法人九州大学 核酸デリバリー方法
US9488645B2 (en) * 2005-09-09 2016-11-08 Jianhui Rong Cell-based microarrays and methods of use
WO2007042771A1 (en) * 2005-10-10 2007-04-19 Astrazeneca Ab Three hybrid screening assay using reverse transfected mammalian cell arrays
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
US8252755B2 (en) * 2006-09-22 2012-08-28 Dharmacon, Inc. Duplex oligonucleotide complexes and methods for gene silencing by RNA interference
US7687267B2 (en) * 2006-09-30 2010-03-30 Rational Biotechnology Inc. High-throughput cell transfection device and methods of using thereof
WO2008071430A1 (en) * 2006-12-13 2008-06-19 Qiagen Gmbh Transfection microarrays
US8834918B2 (en) * 2007-01-22 2014-09-16 Wisconsin Alumni Research Foundation Modified multilayered film
EP2135080A4 (de) * 2007-03-08 2010-12-01 Switchgear Genomics Funktionelle arrays zur charakterisierung mit hohem durchsatz von regulatorischen elementen in nichttranslatierten bereichen von genen
US20110165664A1 (en) * 2007-08-24 2011-07-07 Cytopathfinder, Inc. Transfection device utilizing sericin
WO2009049100A2 (en) * 2007-10-09 2009-04-16 Wisconsin Alumni Research Foundation Ultrathin multilayered films for controlled release of anionic reagents
US7845686B2 (en) * 2007-12-17 2010-12-07 S & B Technical Products, Inc. Restrained pipe joining system for plastic pipe
US8188060B2 (en) 2008-02-11 2012-05-29 Dharmacon, Inc. Duplex oligonucleotides with enhanced functionality in gene regulation
US8324333B2 (en) * 2008-06-05 2012-12-04 Wisconsin Alumni Research Foundation Anionic charge-dynamic polymers for release of cationic agents
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
CN101787375B (zh) * 2010-02-10 2012-08-22 浙江大学 一种反向非病毒载体基因转染的方法
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2752237B1 (de) 2011-07-25 2016-03-16 Csir Array-druckverfahren
US9012375B2 (en) 2011-07-25 2015-04-21 Csir Array printing
US9029297B2 (en) * 2011-07-25 2015-05-12 Csir Array printing
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
LT2981616T (lt) * 2013-07-15 2018-11-26 Universität Heidelberg Baltymų ir nukleino rūgščių kietos fazės transfekavimas
CA2970900A1 (en) * 2014-12-15 2016-06-23 Novellusdx Ltd. Systems, devices, kits and methods for indirect transfection of multiple sets of nucleic-acids and transfer of molecules
JP6828007B2 (ja) 2015-04-10 2021-02-10 スペーシャル トランスクリプトミクス アクチボラグ 生物学的試料の空間識別されるマルチプレックスな核酸分析
US20220025446A1 (en) 2018-12-10 2022-01-27 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
AU2946295A (en) 1994-06-27 1996-01-19 Johns Hopkins University, The Targeted gene delivery system
US5811274A (en) 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
JP3867160B2 (ja) * 1995-07-03 2007-01-10 大日本住友製薬株式会社 遺伝子製剤
US5851818A (en) * 1996-05-31 1998-12-22 Sequus Pharmaceuticals, Inc. Condensed plasmid-liposome complex for transfection
US6133026A (en) 1996-05-31 2000-10-17 Sequus Pharmaceuticals, Inc. Condensed plasmid-liposome complex for transfection
WO1999055886A1 (en) 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
CA2326390A1 (en) 1998-04-30 1999-11-11 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Novel method for the identification of clones conferring a desired biological property from an expression library
JP2002513589A (ja) 1998-04-30 2002-05-14 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ 再アレー化を伴う、発現ライブラリーのクローンを選択するための新規方法
GB9908681D0 (en) 1999-04-16 1999-06-09 Central Research Lab Ltd Apparatus for, and method of, introducing a substance into an object

Also Published As

Publication number Publication date
EP1218529B1 (de) 2006-11-22
DE60031997D1 (de) 2007-01-04
JP2003509060A (ja) 2003-03-11
EP1218529A1 (de) 2002-07-03
US6544790B1 (en) 2003-04-08
WO2001020015A9 (en) 2002-10-03
WO2001020015A1 (en) 2001-03-22
CA2383423A1 (en) 2001-03-22

Similar Documents

Publication Publication Date Title
ATE346156T1 (de) Umkehrtransfektionsverfahren
WO2002077264A3 (en) Arrayed transfection method and uses related thereto
ATE412002T1 (de) Gruppen zum einfangen von nukleinsäuren
NO994009L (no) Gensekvensator og metoder
AU4007401A (en) Function homology screening
ATE110415T1 (de) Verbessertes verfahren und apparat, um biologische substanzen in lebenden zellen einzuführen.
EP1032705A4 (de) Sondenanordnungen und deren verwendungen für verfahren zur dns-unterscheidung
ID18631A (id) Proses pembuatan asam l-amino melalui fermentasi
NZ538852A (en) Selection and isolation of living cells using mRNA-binding probes
ID22863A (id) Metoda untuk memproduksi bahan asam nukleat
DK1085083T3 (da) Dyrkningsmedium for dyreceller og fremgangsmåde til proteinfremstilling under anvendelse af dette
AU716500B2 (en) Nucleic acid constructs for the cell cycle-regulated expression of genes, cells containing such constructs and their use for preparing drugs
DE60126115D1 (de) Oberflächentransfektion und expressionsverfahren
WO2002037944A3 (en) Multiplexed cell analysis system
NO993297D0 (no) Fast dyrkningsmedium for mikroorganismer, fremgangsmÕte for fremstilling herav og anvendelse herav
DE69930515D1 (de) Polyketide, ihre herstellung, und darin benutzte materialien
NO20000576D0 (no) Blanding og fremgangsmåte for regulering av celleproliferasjon og celledoed
AU5957100A (en) Novel mammalian calcium channels and related probes, cell lines and methods
WO2003089600A3 (en) Surface transfection and expression procedure
EP1583835A4 (de) Dna-array für festphasentransfektion mit hohem durchsatz und verfahren zur herstellung davon
HUP0202317A2 (en) Method for cultivating cells, a membrane module utilization of a membrane module and reaction system for cultivation of said cells
TR199600902A2 (tr) Hücre transfeksiyonu.
AU4196999A (en) Rna channels in biological membranes
ATE120493T1 (de) Methode und apparat, um material in biologische zellen einzufügen.
EP1250156A4 (de) Verfahren zur transfektion von nukleinsäure in zellen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties