MX9708967A - Metodo para la purificacion de plasmidos a gran escala. - Google Patents
Metodo para la purificacion de plasmidos a gran escala.Info
- Publication number
- MX9708967A MX9708967A MX9708967A MX9708967A MX9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A
- Authority
- MX
- Mexico
- Prior art keywords
- large scale
- plasmid purification
- scale plasmid
- disclosed
- plasmid dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se describe un procedimiento para aislar y purificar a gran escala ADN de plásmido a partir de fermentaciones microbianas a gran escala; las tres formas de ADN de plásmido, superenrollada (forma I), mellada o de círculo relajado (forma II) y lineal (forma III), se aislan individualmente utilizando el procedimiento descrito; se provee ADN altamente purificado adecuado para incluirlo en una composicion farmacéutica mediante el procedimiento descrito.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44611895A | 1995-05-19 | 1995-05-19 | |
PCT/US1996/007083 WO1996036706A1 (en) | 1995-05-19 | 1996-05-15 | A method for large scale plasmid purification |
Publications (1)
Publication Number | Publication Date |
---|---|
MX9708967A true MX9708967A (es) | 1998-03-31 |
Family
ID=23771380
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX9708967A MX9708967A (es) | 1995-05-19 | 1996-05-15 | Metodo para la purificacion de plasmidos a gran escala. |
Country Status (18)
Country | Link |
---|---|
EP (1) | EP0827536A1 (es) |
JP (1) | JPH11505707A (es) |
KR (1) | KR19990014924A (es) |
CN (1) | CN1190435A (es) |
AR (1) | AR003122A1 (es) |
AU (1) | AU709003B2 (es) |
CA (1) | CA2220867A1 (es) |
CZ (1) | CZ366197A3 (es) |
EA (1) | EA000785B1 (es) |
HR (1) | HRP960222A2 (es) |
HU (1) | HUP9802557A3 (es) |
MX (1) | MX9708967A (es) |
NO (1) | NO975280L (es) |
NZ (1) | NZ309231A (es) |
PL (1) | PL323475A1 (es) |
SK (1) | SK155797A3 (es) |
WO (1) | WO1996036706A1 (es) |
YU (1) | YU29796A (es) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996002658A1 (en) * | 1994-07-15 | 1996-02-01 | Merck & Co., Inc. | A method for large scale plasmid purification |
US6197553B1 (en) | 1994-07-15 | 2001-03-06 | Merck & Co., Inc. | Method for large scale plasmid purification |
GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
US5981735A (en) | 1996-02-12 | 1999-11-09 | Cobra Therapeutics Limited | Method of plasmid DNA production and purification |
FR2753204B1 (fr) * | 1996-09-11 | 1998-12-04 | Transgene Sa | Procede de preparation d'adn plasmidique |
SE9703532D0 (sv) | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
WO1999063076A1 (en) * | 1998-06-01 | 1999-12-09 | The Immune Response Corporation | Novel method of large scale plasmid purification |
US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
US6270970B1 (en) | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
ATE309344T1 (de) | 2001-01-19 | 2005-11-15 | Mueller Drm Ag | Verfahren zur aufbereitung von biomasse zur herstellung von plasmid dna haltigem zelllysat |
GB0107634D0 (en) | 2001-03-27 | 2001-05-16 | Fermentas Ab | Nucleic acid purification |
US6406892B1 (en) | 2001-05-23 | 2002-06-18 | Bio-Rad Laboratories, Inc. | Acetate-free purification of plasmid DNA on hydroxyapatite |
US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
AU2006211216B2 (en) | 2005-01-31 | 2011-02-03 | Merck Sharp & Dohme Llc | Purification process for plasmid DNA |
JP2006238822A (ja) * | 2005-03-04 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
DE102005059315A1 (de) * | 2005-12-09 | 2007-06-14 | Qiagen Gmbh | Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren |
CN100439497C (zh) * | 2006-05-26 | 2008-12-03 | 吉林大学 | 一种适用于大规模生产质粒dna的纯化方法 |
US9163229B2 (en) | 2006-10-10 | 2015-10-20 | Trovagene, Inc. | Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media |
WO2008148010A1 (en) * | 2007-05-23 | 2008-12-04 | Vgx Pharmaceuticals, Inc. | Composition comprising high concentration of biologically active molecules and processes for preparing the same |
CN101070543B (zh) * | 2007-06-01 | 2010-12-29 | 广东药学院 | 超螺旋质粒分离纯化工艺 |
GB0913160D0 (en) * | 2009-07-29 | 2009-09-02 | Avecia Biolog Ltd | Extraction process |
US20130030165A1 (en) | 2010-04-08 | 2013-01-31 | Qiagen Gmbh | Chromatographic device and method for isolating and purifying nucleic acids |
EP2556143B1 (en) * | 2010-04-08 | 2016-12-07 | Qiagen GmbH | Method for isolating and purifying nucleic acids |
JP5624487B2 (ja) | 2011-01-31 | 2014-11-12 | 横河電機株式会社 | 核酸抽出方法 |
EP2702136B1 (en) * | 2011-04-27 | 2017-10-25 | Merck Patent GmbH | Method for lysing cells |
CN104099321A (zh) * | 2014-07-10 | 2014-10-15 | 广州市军科泰特医药科技有限公司 | 抗肺癌T-VISA-BikDD质粒的制备方法 |
CN106884011B (zh) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | 一种规模化质粒纯化的联合液相层析分离方法 |
CN111721871A (zh) * | 2020-06-24 | 2020-09-29 | 南京济群生物科技有限公司 | 一种质粒超螺旋dna含量的高分离度检测方法 |
CN112798697B (zh) * | 2020-11-16 | 2022-11-22 | 武汉波睿达生物科技有限公司 | 慢病毒包装***辅助质粒的超螺旋结构纯度的检测方法 |
CN114456940B (zh) * | 2022-01-23 | 2024-05-03 | 和元生物技术(上海)股份有限公司 | 一种提高大肠杆菌裂解过程中质粒稳定性的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0800830A3 (en) * | 1989-11-03 | 1999-03-17 | Vanderbilt University | Method of in vivo delivery of functioning foreign genes |
WO1993024640A2 (en) * | 1992-06-04 | 1993-12-09 | The Regents Of The University Of California | Methods and compositions for in vivo gene therapy |
US5834441A (en) * | 1993-09-13 | 1998-11-10 | Rhone-Poulenc Rorer Pharmaceuticals Inc. | Adeno-associated viral (AAV) liposomes and methods related thereto |
WO1996002658A1 (en) * | 1994-07-15 | 1996-02-01 | Merck & Co., Inc. | A method for large scale plasmid purification |
-
1996
- 1996-05-09 AR ARP960102507A patent/AR003122A1/es unknown
- 1996-05-15 CA CA002220867A patent/CA2220867A1/en not_active Abandoned
- 1996-05-15 PL PL96323475A patent/PL323475A1/xx unknown
- 1996-05-15 SK SK1557-97A patent/SK155797A3/sk unknown
- 1996-05-15 NZ NZ309231A patent/NZ309231A/en unknown
- 1996-05-15 JP JP8535061A patent/JPH11505707A/ja not_active Withdrawn
- 1996-05-15 HU HU9802557A patent/HUP9802557A3/hu unknown
- 1996-05-15 MX MX9708967A patent/MX9708967A/es not_active IP Right Cessation
- 1996-05-15 EP EP96916486A patent/EP0827536A1/en not_active Withdrawn
- 1996-05-15 WO PCT/US1996/007083 patent/WO1996036706A1/en not_active Application Discontinuation
- 1996-05-15 EA EA199700399A patent/EA000785B1/ru not_active IP Right Cessation
- 1996-05-15 CZ CZ973661A patent/CZ366197A3/cs unknown
- 1996-05-15 HR HR08/446,118A patent/HRP960222A2/hr not_active Application Discontinuation
- 1996-05-15 KR KR1019970708270A patent/KR19990014924A/ko not_active Application Discontinuation
- 1996-05-15 AU AU59219/96A patent/AU709003B2/en not_active Ceased
- 1996-05-15 CN CN96195329A patent/CN1190435A/zh active Pending
- 1996-05-17 YU YU29796A patent/YU29796A/sh unknown
-
1997
- 1997-11-18 NO NO975280A patent/NO975280L/no unknown
Also Published As
Publication number | Publication date |
---|---|
KR19990014924A (ko) | 1999-02-25 |
HRP960222A2 (en) | 1997-12-31 |
NO975280L (no) | 1998-01-16 |
HUP9802557A2 (hu) | 1999-02-01 |
NO975280D0 (no) | 1997-11-18 |
JPH11505707A (ja) | 1999-05-25 |
CN1190435A (zh) | 1998-08-12 |
AR003122A1 (es) | 1998-07-08 |
HUP9802557A3 (en) | 2000-04-28 |
AU5921996A (en) | 1996-11-29 |
EP0827536A1 (en) | 1998-03-11 |
SK155797A3 (en) | 1998-07-08 |
YU29796A (sh) | 1999-06-15 |
AU709003B2 (en) | 1999-08-19 |
NZ309231A (en) | 1999-10-28 |
CA2220867A1 (en) | 1996-11-21 |
CZ366197A3 (cs) | 1998-04-15 |
EA199700399A1 (ru) | 1998-06-25 |
WO1996036706A1 (en) | 1996-11-21 |
EA000785B1 (ru) | 2000-04-24 |
PL323475A1 (en) | 1998-03-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration | ||
MM | Annulment or lapse due to non-payment of fees |