MX9708967A - Metodo para la purificacion de plasmidos a gran escala. - Google Patents

Metodo para la purificacion de plasmidos a gran escala.

Info

Publication number
MX9708967A
MX9708967A MX9708967A MX9708967A MX9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A MX 9708967 A MX9708967 A MX 9708967A
Authority
MX
Mexico
Prior art keywords
large scale
plasmid purification
scale plasmid
disclosed
plasmid dna
Prior art date
Application number
MX9708967A
Other languages
English (en)
Inventor
Ann L Lee
Sangeetha Sagar
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of MX9708967A publication Critical patent/MX9708967A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describe un procedimiento para aislar y purificar a gran escala ADN de plásmido a partir de fermentaciones microbianas a gran escala; las tres formas de ADN de plásmido, superenrollada (forma I), mellada o de círculo relajado (forma II) y lineal (forma III), se aislan individualmente utilizando el procedimiento descrito; se provee ADN altamente purificado adecuado para incluirlo en una composicion farmacéutica mediante el procedimiento descrito.
MX9708967A 1995-05-19 1996-05-15 Metodo para la purificacion de plasmidos a gran escala. MX9708967A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44611895A 1995-05-19 1995-05-19
PCT/US1996/007083 WO1996036706A1 (en) 1995-05-19 1996-05-15 A method for large scale plasmid purification

Publications (1)

Publication Number Publication Date
MX9708967A true MX9708967A (es) 1998-03-31

Family

ID=23771380

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9708967A MX9708967A (es) 1995-05-19 1996-05-15 Metodo para la purificacion de plasmidos a gran escala.

Country Status (18)

Country Link
EP (1) EP0827536A1 (es)
JP (1) JPH11505707A (es)
KR (1) KR19990014924A (es)
CN (1) CN1190435A (es)
AR (1) AR003122A1 (es)
AU (1) AU709003B2 (es)
CA (1) CA2220867A1 (es)
CZ (1) CZ366197A3 (es)
EA (1) EA000785B1 (es)
HR (1) HRP960222A2 (es)
HU (1) HUP9802557A3 (es)
MX (1) MX9708967A (es)
NO (1) NO975280L (es)
NZ (1) NZ309231A (es)
PL (1) PL323475A1 (es)
SK (1) SK155797A3 (es)
WO (1) WO1996036706A1 (es)
YU (1) YU29796A (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996002658A1 (en) * 1994-07-15 1996-02-01 Merck & Co., Inc. A method for large scale plasmid purification
US6197553B1 (en) 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
FR2753204B1 (fr) * 1996-09-11 1998-12-04 Transgene Sa Procede de preparation d'adn plasmidique
SE9703532D0 (sv) 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
WO1999063076A1 (en) * 1998-06-01 1999-12-09 The Immune Response Corporation Novel method of large scale plasmid purification
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
ATE309344T1 (de) 2001-01-19 2005-11-15 Mueller Drm Ag Verfahren zur aufbereitung von biomasse zur herstellung von plasmid dna haltigem zelllysat
GB0107634D0 (en) 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
AU2006211216B2 (en) 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
JP2006238822A (ja) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
DE102005059315A1 (de) * 2005-12-09 2007-06-14 Qiagen Gmbh Verfahren zur Anreicherung von kurzkettigen Nukleinsäuren
CN100439497C (zh) * 2006-05-26 2008-12-03 吉林大学 一种适用于大规模生产质粒dna的纯化方法
US9163229B2 (en) 2006-10-10 2015-10-20 Trovagene, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
WO2008148010A1 (en) * 2007-05-23 2008-12-04 Vgx Pharmaceuticals, Inc. Composition comprising high concentration of biologically active molecules and processes for preparing the same
CN101070543B (zh) * 2007-06-01 2010-12-29 广东药学院 超螺旋质粒分离纯化工艺
GB0913160D0 (en) * 2009-07-29 2009-09-02 Avecia Biolog Ltd Extraction process
US20130030165A1 (en) 2010-04-08 2013-01-31 Qiagen Gmbh Chromatographic device and method for isolating and purifying nucleic acids
EP2556143B1 (en) * 2010-04-08 2016-12-07 Qiagen GmbH Method for isolating and purifying nucleic acids
JP5624487B2 (ja) 2011-01-31 2014-11-12 横河電機株式会社 核酸抽出方法
EP2702136B1 (en) * 2011-04-27 2017-10-25 Merck Patent GmbH Method for lysing cells
CN104099321A (zh) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 抗肺癌T-VISA-BikDD质粒的制备方法
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法
CN112798697B (zh) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 慢病毒包装***辅助质粒的超螺旋结构纯度的检测方法
CN114456940B (zh) * 2022-01-23 2024-05-03 和元生物技术（上海）股份有限公司 一种提高大肠杆菌裂解过程中质粒稳定性的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0800830A3 (en) * 1989-11-03 1999-03-17 Vanderbilt University Method of in vivo delivery of functioning foreign genes
WO1993024640A2 (en) * 1992-06-04 1993-12-09 The Regents Of The University Of California Methods and compositions for in vivo gene therapy
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
WO1996002658A1 (en) * 1994-07-15 1996-02-01 Merck & Co., Inc. A method for large scale plasmid purification

Also Published As

Publication number Publication date
KR19990014924A (ko) 1999-02-25
HRP960222A2 (en) 1997-12-31
NO975280L (no) 1998-01-16
HUP9802557A2 (hu) 1999-02-01
NO975280D0 (no) 1997-11-18
JPH11505707A (ja) 1999-05-25
CN1190435A (zh) 1998-08-12
AR003122A1 (es) 1998-07-08
HUP9802557A3 (en) 2000-04-28
AU5921996A (en) 1996-11-29
EP0827536A1 (en) 1998-03-11
SK155797A3 (en) 1998-07-08
YU29796A (sh) 1999-06-15
AU709003B2 (en) 1999-08-19
NZ309231A (en) 1999-10-28
CA2220867A1 (en) 1996-11-21
CZ366197A3 (cs) 1998-04-15
EA199700399A1 (ru) 1998-06-25
WO1996036706A1 (en) 1996-11-21
EA000785B1 (ru) 2000-04-24
PL323475A1 (en) 1998-03-30

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Legal Events

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FG Grant or registration
MM Annulment or lapse due to non-payment of fees