ATE274522T1 - Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographie - Google Patents
Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographieInfo
- Publication number
- ATE274522T1 ATE274522T1 AT99925856T AT99925856T ATE274522T1 AT E274522 T1 ATE274522 T1 AT E274522T1 AT 99925856 T AT99925856 T AT 99925856T AT 99925856 T AT99925856 T AT 99925856T AT E274522 T1 ATE274522 T1 AT E274522T1
- Authority
- AT
- Austria
- Prior art keywords
- multimers
- exchange chromatography
- separation
- ion exchange
- mixture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
- C07K16/4283—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
- C07K16/4291—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8760298P | 1998-06-01 | 1998-06-01 | |
PCT/US1999/011598 WO1999062936A1 (en) | 1998-06-01 | 1999-05-24 | Separation of protein monomers from aggregates by use of ion-exchange chromatography |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE274522T1 true ATE274522T1 (de) | 2004-09-15 |
Family
ID=22206161
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT99925856T ATE274522T1 (de) | 1998-06-01 | 1999-05-24 | Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographie |
Country Status (13)
Country | Link |
---|---|
US (2) | US6620918B2 (de) |
EP (2) | EP1500661A1 (de) |
JP (3) | JP2002517406A (de) |
AT (1) | ATE274522T1 (de) |
AU (1) | AU750977B2 (de) |
CA (1) | CA2333747C (de) |
DE (1) | DE69919701T2 (de) |
DK (1) | DK1084136T3 (de) |
ES (1) | ES2228052T3 (de) |
HK (1) | HK1037879A1 (de) |
IL (2) | IL139609A (de) |
PT (1) | PT1084136E (de) |
WO (1) | WO1999062936A1 (de) |
Families Citing this family (49)
Publication number | Priority date | Publication date | Assignee | Title |
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CA2338665C (en) * | 1998-08-06 | 2011-01-18 | Mountain View Pharmaceuticals, Inc. | Peg-urate oxidase conjugates and use thereof |
US6783965B1 (en) * | 2000-02-10 | 2004-08-31 | Mountain View Pharmaceuticals, Inc. | Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates |
JP4798832B2 (ja) * | 2000-10-24 | 2011-10-19 | 一般財団法人化学及血清療法研究所 | ヒト血清アルブミン多量体の除去方法 |
GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
AU2003901507A0 (en) * | 2003-03-28 | 2003-04-17 | Norika Holdings | Process for isolating a pharmaceutical product |
KR101412271B1 (ko) * | 2003-05-09 | 2014-06-25 | 듀크 유니버시티 | Cd20-특이적 항체 및 이를 이용한 방법 |
US20050065136A1 (en) * | 2003-08-13 | 2005-03-24 | Roby Russell R. | Methods and compositions for the treatment of infertility using dilute hormone solutions |
WO2005077130A2 (en) * | 2004-02-11 | 2005-08-25 | Tanox, Inc. | A method for the removal of aggregate proteins from recombinant samples using ion exchange chromatography |
US20050239757A1 (en) * | 2004-04-21 | 2005-10-27 | Roby Russell R | Hormone treatment of macular degeneration |
US20060025390A1 (en) * | 2004-07-28 | 2006-02-02 | Roby Russell R | Treatment of hormone allergy and related symptoms and disorders |
KR20070072510A (ko) * | 2004-08-30 | 2007-07-04 | 론자 바이올로직스 피엘씨 | 항체 정제를 위한 친화도- 및 이온 교환- 크로마토그래피 |
JP4498186B2 (ja) * | 2005-03-24 | 2010-07-07 | 株式会社日立ハイテクノロジーズ | 液体クロマトグラフ分析方法及び装置 |
US20080159976A1 (en) * | 2005-04-11 | 2008-07-03 | Jacob Hartman | Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase |
ES2856881T3 (es) | 2005-04-11 | 2021-09-28 | Horizon Pharma Rheumatology Llc | Formas variantes de urato oxidasa y su uso |
EP1871877A2 (de) | 2005-04-11 | 2008-01-02 | Savient Pharmaceuticals, Inc. | Nebenform von uratoxidase und ihre verwendung |
US8148123B2 (en) * | 2005-04-11 | 2012-04-03 | Savient Pharmaceuticals, Inc. | Methods for lowering elevated uric acid levels using intravenous injections of PEG-uricase |
EP1883425A1 (de) * | 2005-05-23 | 2008-02-06 | Universite De Geneve | Injizierbare superparamagnetische nanoteilchen zur behandlung von hyperthermie und verwendung zur bildung eines hyperthermischen implantats |
TWI320788B (en) | 2005-05-25 | 2010-02-21 | Hoffmann La Roche | Method for the purification of antibodies |
AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
ES2439641T3 (es) | 2005-12-20 | 2014-01-24 | Bristol-Myers Squibb Company | Composiciones y procedimientos de producción de una composición |
US20080032343A1 (en) | 2005-12-22 | 2008-02-07 | Genentech, Inc. | Recombinant Production of Heparin Binding Proteins |
ES2532804T3 (es) * | 2006-04-12 | 2015-03-31 | Crealta Pharmaceuticals Llc | Purificación de proteínas con tensioactivo catiónico |
WO2007123242A1 (ja) | 2006-04-25 | 2007-11-01 | Tosoh Corporation | IgG精製用分離剤、及びそれを用いたIgG単量体の精製方法 |
RU2439076C2 (ru) | 2006-07-14 | 2012-01-10 | Дженентек, Инк. | Способ выделения повторно свернутого рекомбинантного белка из культуры прокариотических клеток (варианты) |
FI2061803T4 (fi) | 2006-08-28 | 2023-03-14 | Process for the purification of fc-containing proteins | |
SG184699A1 (en) | 2007-03-23 | 2012-10-30 | Asahi Kasei Pharma Corp | Method for producing high-purity soluble thrombomodulin |
ITLU20070007A1 (it) * | 2007-05-07 | 2008-11-08 | Kedrion Spa | Processo cromatografico per l'ottenimento di un complesso fviii/vwf con differenti rapporti tra le due proteine da utilizzare nella terapia della emofilia a e nella malattia di von willebrand. |
AU2008256411B2 (en) * | 2007-06-01 | 2013-08-22 | F. Hoffmann-La Roche Ag | Immunoglobulin purification |
JP5778389B2 (ja) | 2007-10-26 | 2015-09-16 | 旭化成ケミカルズ株式会社 | たんぱく質の精製方法 |
SG175597A1 (en) | 2007-10-30 | 2011-11-28 | Genentech Inc | Antibody purification by cation exchange chromatography |
JP2012513425A (ja) | 2008-12-22 | 2012-06-14 | エフ.ホフマン−ラ ロシュ アーゲー | 免疫グロブリンの精製 |
AU2010265964B2 (en) | 2009-06-25 | 2014-09-18 | Horizon Therapeutics Usa, Inc. | Methods and kits for predicting infusion reaction risk and antibody-mediated loss of response by monitoring serum uric acid during PEGylated uricase therapy |
JPWO2011001963A1 (ja) | 2009-07-03 | 2012-12-13 | 旭化成ケミカルズ株式会社 | 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法 |
US8697844B2 (en) | 2009-11-24 | 2014-04-15 | Novo Nordisk A/S | Method of purifying pegylated proteins |
WO2011073389A1 (en) | 2009-12-18 | 2011-06-23 | Novartis Ag | Wash solution and method for affinity chromatography |
RU2012146448A (ru) | 2010-04-14 | 2014-05-20 | Ф.Хоффманн-Ля Рош Аг | Удаление агрегатов иммуноглобулинов |
CN101948528B (zh) * | 2010-09-26 | 2012-02-01 | 广东工业大学 | 一种来自斑蝥的纤溶活性蛋白及其制备方法和应用 |
SG193378A1 (en) | 2011-03-16 | 2013-10-30 | Hoffmann La Roche | Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase |
PL221899B1 (pl) | 2011-05-06 | 2016-06-30 | Wrocławskie Centrum Badań Eit + Spółka Z Ograniczoną | Czysta albumina oraz sposób jej otrzymywania i detekcji |
SG10201701224UA (en) | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
KR101516054B1 (ko) * | 2012-11-21 | 2015-05-06 | 씨제이헬스케어 주식회사 | 신규한 지속형 인간 성장호르몬 단량체를 제조하는 방법 |
JP6285532B2 (ja) | 2013-03-14 | 2018-02-28 | イー・エム・デイー・ミリポア・コーポレイシヨン | タンパク質aに基づくクロマトグラフィを使用するタンパク質純度を増加させる方法 |
AR096713A1 (es) * | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
KR101975771B1 (ko) | 2013-12-12 | 2019-05-09 | 이엠디 밀리포어 코포레이션 | 아크릴아미드 함유 필터를 사용한 단백질 분리 |
EP3290432B1 (de) * | 2015-04-28 | 2022-03-30 | Hybio Pharmaceutical Co., Ltd. | Hochleistungsflüssigchromatografieverfahren für polypeptidmischungen |
CA2992419C (en) * | 2015-07-14 | 2023-08-15 | Yeda Research And Development Co. Ltd. | Peptide combinations for use in the diagnosis of schizophrenia |
JP6749590B2 (ja) * | 2015-09-17 | 2020-09-02 | 国立大学法人 東京大学 | 酸化型及び還元型アルブミンの分析方法、酸化型及び還元型アルブミンの分析装置、及び酸化型及び還元型アルブミンの分析キット |
AR114722A1 (es) | 2018-04-02 | 2020-10-07 | Amgen Inc | Composiciones de erenumab y usos de las mismas |
IL307236A (en) | 2021-03-31 | 2023-11-01 | Hoffmann La Roche | Purification of antibodies by mixed-mode chromatography |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
FR2548670B1 (fr) * | 1983-07-07 | 1985-10-25 | Merieux Inst | Procede de preparation des principales proteines du sang hemolyse sous forme non denaturee |
US5196323A (en) * | 1985-04-27 | 1993-03-23 | Boehringer Ingelheim International Gmbh | Process for preparing and purifying alpha-interferon |
US4765903A (en) * | 1987-10-06 | 1988-08-23 | Interferon Sciences, Inc. | Purification of monomeric interferon |
ES2224094T3 (es) * | 1990-04-19 | 2005-03-01 | Bayer Corporation | Procedimiento para preparar seroalbumina normal (humana) esencialmente monomerica. |
US5250663A (en) * | 1990-04-19 | 1993-10-05 | Miles Inc. | Preparing essentially monomeric normal human serum albumin |
US5110913A (en) | 1990-05-25 | 1992-05-05 | Miles Inc. | Antibody purification method |
US5256294A (en) * | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
US5231178A (en) | 1991-01-16 | 1993-07-27 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Method for the purification of intact, correctly-folded insulin-like growth factor-1 |
DE4110974A1 (de) * | 1991-04-05 | 1992-10-08 | Bayer Ag | Hochkautschukhaltige polymerkombinationen |
JPH07285885A (ja) * | 1994-04-21 | 1995-10-31 | Kyodo Nyugyo Kk | 免疫グロブリンの製造法 |
ATE253079T1 (de) | 1995-06-07 | 2003-11-15 | Chiron Corp | Methoden zur aufreinigung von authentischem igf aus hefewirtszellen |
TW505655B (en) * | 1997-10-14 | 2002-10-11 | Tanox Inc | Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography |
BRPI9910332B8 (pt) | 1998-05-06 | 2021-05-25 | Genentech Inc | método para purificação de um polipeptídio a partir de uma composição |
-
1999
- 1999-05-24 EP EP04017970A patent/EP1500661A1/de not_active Withdrawn
- 1999-05-24 JP JP2000552146A patent/JP2002517406A/ja not_active Ceased
- 1999-05-24 AT AT99925856T patent/ATE274522T1/de not_active IP Right Cessation
- 1999-05-24 CA CA002333747A patent/CA2333747C/en not_active Expired - Fee Related
- 1999-05-24 DK DK99925856T patent/DK1084136T3/da active
- 1999-05-24 IL IL139609A patent/IL139609A/en not_active IP Right Cessation
- 1999-05-24 PT PT99925856T patent/PT1084136E/pt unknown
- 1999-05-24 WO PCT/US1999/011598 patent/WO1999062936A1/en not_active Application Discontinuation
- 1999-05-24 ES ES99925856T patent/ES2228052T3/es not_active Expired - Lifetime
- 1999-05-24 DE DE69919701T patent/DE69919701T2/de not_active Revoked
- 1999-05-24 EP EP99925856A patent/EP1084136B1/de not_active Revoked
- 1999-05-26 US US09/320,100 patent/US6620918B2/en not_active Expired - Lifetime
- 1999-05-26 AU AU42059/99A patent/AU750977B2/en not_active Ceased
-
2001
- 2001-09-21 HK HK01106713A patent/HK1037879A1/xx not_active IP Right Cessation
-
2003
- 2003-08-15 US US10/642,409 patent/US20040116674A1/en not_active Abandoned
-
2004
- 2004-01-08 JP JP2004003506A patent/JP2004175806A/ja not_active Withdrawn
-
2005
- 2005-09-22 JP JP2005276944A patent/JP2006071646A/ja active Pending
-
2006
- 2006-01-30 IL IL173432A patent/IL173432A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO1999062936A1 (en) | 1999-12-09 |
DE69919701D1 (de) | 2004-09-30 |
EP1084136A1 (de) | 2001-03-21 |
US6620918B2 (en) | 2003-09-16 |
CA2333747A1 (en) | 1999-12-09 |
DE69919701T2 (de) | 2005-09-15 |
DK1084136T3 (da) | 2005-01-03 |
JP2006071646A (ja) | 2006-03-16 |
AU750977B2 (en) | 2002-08-01 |
PT1084136E (pt) | 2004-12-31 |
US20040116674A1 (en) | 2004-06-17 |
JP2004175806A (ja) | 2004-06-24 |
US20020010319A1 (en) | 2002-01-24 |
IL139609A0 (en) | 2002-02-10 |
EP1084136B1 (de) | 2004-08-25 |
CA2333747C (en) | 2008-02-19 |
IL173432A0 (en) | 2006-06-11 |
IL139609A (en) | 2006-06-11 |
ES2228052T3 (es) | 2005-04-01 |
HK1037879A1 (en) | 2002-02-22 |
JP2002517406A (ja) | 2002-06-18 |
AU4205999A (en) | 1999-12-20 |
EP1500661A1 (de) | 2005-01-26 |
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Legal Events
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UEP | Publication of translation of european patent specification |
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RZN | Patent revoked |