ATE247708T1 - Verfahren zur regulierung der metabolischen stabilität von proteinen - Google Patents
Verfahren zur regulierung der metabolischen stabilität von proteinenInfo
- Publication number
- ATE247708T1 ATE247708T1 AT87907061T AT87907061T ATE247708T1 AT E247708 T1 ATE247708 T1 AT E247708T1 AT 87907061 T AT87907061 T AT 87907061T AT 87907061 T AT87907061 T AT 87907061T AT E247708 T1 ATE247708 T1 AT E247708T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- methods
- metabolic stability
- amino
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
- C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US91515186A | 1986-10-02 | 1986-10-02 | |
PCT/US1987/002522 WO1988002406A2 (en) | 1986-10-02 | 1987-10-01 | Methods of regulating metabolic stability of proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE247708T1 true ATE247708T1 (de) | 2003-09-15 |
Family
ID=25435312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT87907061T ATE247708T1 (de) | 1986-10-02 | 1987-10-01 | Verfahren zur regulierung der metabolischen stabilität von proteinen |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0324789B1 (de) |
JP (4) | JP2612874B2 (de) |
AT (1) | ATE247708T1 (de) |
CA (1) | CA1313831C (de) |
DE (1) | DE3752372T2 (de) |
WO (1) | WO1988002406A2 (de) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5132213A (en) * | 1986-10-02 | 1992-07-21 | Massachusetts Institute Of Technology | Method for producing proteins and polypeptides using ubiquitin fusions |
US5196321A (en) * | 1986-10-02 | 1993-03-23 | Massachusetts Institute Of Technology | Methods for in vitro cleavage of ubiquitin fusion proteins |
US5156968A (en) * | 1988-06-24 | 1992-10-20 | Genentech, Inc. | Purified yeast ubiquitin hydrolase |
US5108919A (en) * | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
US5262322A (en) * | 1988-06-24 | 1993-11-16 | Genentech, Inc. | Host transformed with yeast gene and ubiquitin/polypeptide fusions |
ES2054365T3 (es) * | 1989-06-30 | 1994-08-01 | Massachusetts Inst Technology | Inhibicion de la via de la regla del n final en celulas vivas. |
US6068994A (en) * | 1989-08-07 | 2000-05-30 | Chiron Corporation | Ubiquitin expression system |
US5620923A (en) * | 1989-10-12 | 1997-04-15 | The University Of Utah | Synthesis of peptides as cloned ubiquitin extensions |
ATE121454T1 (de) * | 1990-05-09 | 1995-05-15 | Massachusetts Inst Technology | Ubiquitinspezifische protease. |
US5212058A (en) * | 1990-05-09 | 1993-05-18 | Massachusetts Institute Of Technology | Nucleic acid encoding ubiquitin-specific proteases |
WO1992009698A1 (en) | 1990-11-26 | 1992-06-11 | Genetics Institute, Inc. | Expression of pace in host cells and methods of use thereof |
AT405053B (de) * | 1991-06-10 | 1999-05-25 | Lucky Ltd | Hepatitis-c-diagnosemittel und -impfstoffe |
US5656456A (en) * | 1992-07-13 | 1997-08-12 | Bionebraska, Inc. | Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
DK0651761T3 (da) * | 1992-07-13 | 2003-02-10 | Bionebraska Inc | Fremgangsmåde til modifikation af rekombinante polypeptider |
EP0669833B1 (de) * | 1992-10-29 | 2002-06-12 | Celtrix Pharmaceuticals, Inc. | Typ ii tgf-beta-bindendes rezeptorfragment als therapeutisches mittel |
US5459051A (en) * | 1993-03-26 | 1995-10-17 | Celtrix Pharmaceuticals, Inc. | Methods and vectors for over-expression of ubiquitin fusion proteins in host cells |
US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
US5914254A (en) * | 1993-08-02 | 1999-06-22 | Celtrix Pharmaceuticals, Inc. | Expression of fusion polypeptides transported out of the cytoplasm without leader sequences |
WO1996017941A2 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
US6576469B1 (en) | 1997-09-10 | 2003-06-10 | President And Fellows Of Harvard College | Inducible methods for repressing gene function |
DE69841807D1 (de) | 1997-11-06 | 2010-09-16 | Novartis Vaccines & Diagnostic | Neisseriale antigene |
DK1047784T4 (en) | 1998-01-14 | 2015-06-15 | Novartis Vaccines & Diagnostic | NEISSERA meningitidis ANTIGENS |
GB9808932D0 (en) | 1998-04-27 | 1998-06-24 | Chiron Spa | Polyepitope carrier protein |
EP2261355A3 (de) | 1998-05-01 | 2012-01-11 | Novartis Vaccines and Diagnostics, Inc. | Antigene und Zusammensetzungen gegen Neisseria meningitidis |
AU5204199A (en) * | 1998-06-12 | 1999-12-30 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Compositions and methods for modulating proteolytic degradation of intracellulartargets |
DK1097211T3 (da) * | 1998-07-10 | 2007-06-04 | Calgene Llc | Ekspression af eukaryote peptider i planteplastider |
AU2605600A (en) * | 1999-01-11 | 2000-08-01 | Mindset Biopharmaceuticals (Usa) Inc. | Methods for regulating the stability of recombinant proteins and products usefultherein |
NZ581940A (en) | 1999-04-30 | 2011-07-29 | Novartis Vaccines & Diagnostic | Conserved neisserial antigens |
GB9911683D0 (en) | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
GB9916529D0 (en) | 1999-07-14 | 1999-09-15 | Chiron Spa | Antigenic peptides |
ES2543736T3 (es) | 1999-10-29 | 2015-08-21 | Glaxosmithkline Biologicals Sa | Péptidos antigénicos de Neisseria |
DE60142772D1 (de) | 2000-01-17 | 2010-09-23 | Novartis Vaccines & Diagnostic | Membranvesikel (omv) impfstoff, der n. meningitidis serogruppe b membranproteine enthält |
US7262005B1 (en) * | 2000-02-04 | 2007-08-28 | Aurora Biosciences Corporation | Methods of protein destabilization and uses thereof |
PT1292697E (pt) * | 2000-06-15 | 2009-10-28 | Smithkline Beecham Corp | Método para a preparação de um polipéptido il-18 fisiologicamente activo |
NZ524888A (en) | 2000-10-27 | 2006-09-29 | Chiron S | Nucleic acids and proteins from streptococcus groups A & B |
GB0107661D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Staphylococcus aureus |
GB0107658D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
JPWO2002083907A1 (ja) * | 2001-04-10 | 2004-08-05 | 塩野義製薬株式会社 | UspAを用いた目的ポリペプチドの製造方法 |
EP2168596B1 (de) | 2001-12-12 | 2012-05-23 | Novartis Vaccines and Diagnostics S.r.l. | Immunisierung gegen Chlamydia trachomatis |
DE60332477D1 (de) | 2002-11-15 | 2010-06-17 | Novartis Vaccines & Diagnostic | Unerwartete oberflächenproteine in neisseria meningitidis |
GB0308198D0 (en) | 2003-04-09 | 2003-05-14 | Chiron Srl | ADP-ribosylating bacterial toxin |
WO2009030978A2 (en) | 2006-06-09 | 2009-03-12 | Novartis Ag | Conformers of bacterial adhesins |
KR101873201B1 (ko) * | 2015-06-11 | 2018-07-02 | 주식회사 제넥신 | 변형된 인터루킨-7 단백질 및 이의 용도 |
KR102386735B1 (ko) | 2015-11-06 | 2022-04-14 | 주식회사 제넥신 | 변형된 인터루킨-7 융합 단백질의 제형 |
CN108697764A (zh) | 2015-12-04 | 2018-10-23 | 格纳西尼有限公司 | 含有免疫球蛋白fc融合白细胞介素-7融合蛋白的用于预防或治疗流感病毒感染的药物组合物 |
US11708399B2 (en) | 2015-12-04 | 2023-07-25 | Genexine, Inc. | Pharmaceutical composition comprising immunoglobulin Fc-fused interleukin-7 fusion protein for preventing or treating human papillomavirus-caused diseases |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1985000831A1 (en) * | 1983-08-10 | 1985-02-28 | Amgen | Microbial expression of insulin-like growth factor |
EP0174999B1 (de) * | 1984-03-16 | 1991-04-17 | Biogen, Inc. | Verfahren zur Expressionsverbesserung und Mittel hierfür |
EP0163573B1 (de) * | 1984-05-24 | 1989-09-06 | Merck & Co. Inc. | Positionsspezifische Proteolyse mittels Renin |
JPS62135500A (ja) * | 1985-06-20 | 1987-06-18 | モンサント コンパニ− | ポリペプチドからのペプチドの放出 |
-
1987
- 1987-10-01 DE DE3752372T patent/DE3752372T2/de not_active Expired - Fee Related
- 1987-10-01 WO PCT/US1987/002522 patent/WO1988002406A2/en active IP Right Grant
- 1987-10-01 JP JP62506416A patent/JP2612874B2/ja not_active Expired - Fee Related
- 1987-10-01 EP EP87907061A patent/EP0324789B1/de not_active Expired - Lifetime
- 1987-10-01 AT AT87907061T patent/ATE247708T1/de not_active IP Right Cessation
- 1987-10-02 CA CA000548512A patent/CA1313831C/en not_active Expired - Fee Related
-
1996
- 1996-02-08 JP JP8045655A patent/JP2776414B2/ja not_active Expired - Lifetime
-
1997
- 1997-05-26 JP JP9149916A patent/JP2883594B2/ja not_active Expired - Lifetime
-
1998
- 1998-10-07 JP JP10299072A patent/JP3038193B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA1313831C (en) | 1993-02-23 |
JP2612874B2 (ja) | 1997-05-21 |
DE3752372T2 (de) | 2004-06-24 |
JPH08252096A (ja) | 1996-10-01 |
JPH11187875A (ja) | 1999-07-13 |
JPH02501618A (ja) | 1990-06-07 |
DE3752372D1 (de) | 2003-09-25 |
EP0324789A1 (de) | 1989-07-26 |
WO1988002406A3 (en) | 1988-07-14 |
JP2776414B2 (ja) | 1998-07-16 |
JP3038193B2 (ja) | 2000-05-08 |
WO1988002406A2 (en) | 1988-04-07 |
JP2883594B2 (ja) | 1999-04-19 |
JPH1057061A (ja) | 1998-03-03 |
EP0324789B1 (de) | 2003-08-20 |
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Legal Events
Date | Code | Title | Description |
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UEP | Publication of translation of european patent specification |
Ref document number: 0324789 Country of ref document: EP |
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REN | Ceased due to non-payment of the annual fee |