JPS56125652A - Microorganism measuring method by impedance method - Google Patents

Microorganism measuring method by impedance method

Info

Publication number
JPS56125652A
JPS56125652A JP2858180A JP2858180A JPS56125652A JP S56125652 A JPS56125652 A JP S56125652A JP 2858180 A JP2858180 A JP 2858180A JP 2858180 A JP2858180 A JP 2858180A JP S56125652 A JPS56125652 A JP S56125652A
Authority
JP
Japan
Prior art keywords
impedance
decrease
culture medium
increase
kinds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2858180A
Other languages
Japanese (ja)
Other versions
JPS6312253B2 (en
Inventor
Hidehiko Fujioka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tectron Instruments Corp
Original Assignee
Japan Tectron Instruments Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tectron Instruments Corp filed Critical Japan Tectron Instruments Corp
Priority to JP2858180A priority Critical patent/JPS56125652A/en
Priority to EP19810300928 priority patent/EP0036274B1/en
Priority to DE8181300928T priority patent/DE3175570D1/en
Priority to CA000372494A priority patent/CA1158968A/en
Publication of JPS56125652A publication Critical patent/JPS56125652A/en
Publication of JPS6312253B2 publication Critical patent/JPS6312253B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration

Abstract

PURPOSE:To make characteristic test of microorganisms by measuring the decrease and increase in the impedance of a culture medium caused by the propagation of microorganisms. CONSTITUTION:The culture medium is made by dissolving a basal medium of which the essential components are proteins such as peptone and meat extract used with amino acids in fungus bodies, 2.5-10.0gr/l hydrocarbon such as grape sugar or inositol and 1.0-10.0gr/l salts such as sodium or potassium in distilled water. If such specific culture medium is used, a peculiar impedance change is shown depending upon the kinds of germs. The decrease rate alpha and increase rate beta per unit time, decrease time t1, and increase time t2 of this impedance as well as the impedance decrease rate I at the start of increasing are measured. The data obtained with various kinds of unknown bacteria and known data are compared and the kinds of germs are identified. Since the peculiar impedance change is recognized, the automatic discrimination of identification is possible and since this method is usable for mircroorganism having hydrocarbon utilization caapcity, it is effective in the examination in clinical field.
JP2858180A 1980-03-08 1980-03-08 Microorganism measuring method by impedance method Granted JPS56125652A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2858180A JPS56125652A (en) 1980-03-08 1980-03-08 Microorganism measuring method by impedance method
EP19810300928 EP0036274B1 (en) 1980-03-08 1981-03-05 Methods of monitoring micro-organisms and media for culture
DE8181300928T DE3175570D1 (en) 1980-03-08 1981-03-05 Methods of monitoring micro-organisms and media for culture
CA000372494A CA1158968A (en) 1980-03-08 1981-03-06 Methods of monitoring micro-organisms and media for culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2858180A JPS56125652A (en) 1980-03-08 1980-03-08 Microorganism measuring method by impedance method

Publications (2)

Publication Number Publication Date
JPS56125652A true JPS56125652A (en) 1981-10-02
JPS6312253B2 JPS6312253B2 (en) 1988-03-18

Family

ID=12252558

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2858180A Granted JPS56125652A (en) 1980-03-08 1980-03-08 Microorganism measuring method by impedance method

Country Status (1)

Country Link
JP (1) JPS56125652A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63191046A (en) * 1987-02-04 1988-08-08 Kobe Steel Ltd Measurement of amount of biomass
JPH02114163A (en) * 1988-10-25 1990-04-26 Kobe Steel Ltd Organism culture container
CN106706717A (en) * 2016-11-18 2017-05-24 广西壮族自治区分析测试研究中心 Method for using indirect impedance method to detect acid canned food commercial sterilization

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS503695A (en) * 1973-04-25 1975-01-16

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS503695A (en) * 1973-04-25 1975-01-16

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63191046A (en) * 1987-02-04 1988-08-08 Kobe Steel Ltd Measurement of amount of biomass
JPH07117511B2 (en) * 1987-02-04 1995-12-18 株式会社神戸製鋼所 Method of measuring bacterial cell amount
JPH02114163A (en) * 1988-10-25 1990-04-26 Kobe Steel Ltd Organism culture container
JPH0569463B2 (en) * 1988-10-25 1993-10-01 Kobe Steel Ltd
CN106706717A (en) * 2016-11-18 2017-05-24 广西壮族自治区分析测试研究中心 Method for using indirect impedance method to detect acid canned food commercial sterilization
CN106706717B (en) * 2016-11-18 2020-02-07 广西壮族自治区分析测试研究中心 Method for rapidly detecting commercial sterility of acidic canned food by indirect impedance method

Also Published As

Publication number Publication date
JPS6312253B2 (en) 1988-03-18

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