ATE133450T1 - Cephalosporin acetylhydrolasegen und dadurch kodiertes protein - Google Patents

Cephalosporin acetylhydrolasegen und dadurch kodiertes protein

Info

Publication number
ATE133450T1
ATE133450T1 AT91303789T AT91303789T ATE133450T1 AT E133450 T1 ATE133450 T1 AT E133450T1 AT 91303789 T AT91303789 T AT 91303789T AT 91303789 T AT91303789 T AT 91303789T AT E133450 T1 ATE133450 T1 AT E133450T1
Authority
AT
Austria
Prior art keywords
cephalosporin
gene
protein
enzyme
protein encoded
Prior art date
Application number
AT91303789T
Other languages
English (en)
Inventor
Kenji Mitsushima
Akio Takimoto
Shigeo Yagi
Takayasu Sonoyama
Original Assignee
Shionogi & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi & Co filed Critical Shionogi & Co
Application granted granted Critical
Publication of ATE133450T1 publication Critical patent/ATE133450T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cephalosporin Compounds (AREA)
AT91303789T 1990-04-27 1991-04-26 Cephalosporin acetylhydrolasegen und dadurch kodiertes protein ATE133450T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11348390 1990-04-27

Publications (1)

Publication Number Publication Date
ATE133450T1 true ATE133450T1 (de) 1996-02-15

Family

ID=14613433

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91303789T ATE133450T1 (de) 1990-04-27 1991-04-26 Cephalosporin acetylhydrolasegen und dadurch kodiertes protein

Country Status (15)

Country Link
US (2) US5281525A (de)
EP (1) EP0454478B1 (de)
JP (1) JP2651288B2 (de)
KR (1) KR960014703B1 (de)
CN (2) CN1047202C (de)
AT (1) ATE133450T1 (de)
AU (1) AU638124B2 (de)
CA (1) CA2040707C (de)
DE (1) DE69116593T2 (de)
DK (1) DK0454478T3 (de)
ES (1) ES2085422T3 (de)
GR (1) GR3018790T3 (de)
IL (1) IL97915A (de)
NZ (1) NZ237878A (de)
RU (1) RU2103364C1 (de)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1250698B (it) * 1991-07-24 1995-04-21 Mini Ricerca Scient Tecnolog Processo per la preparazione enzimatica di acidi 7-ammino-cefalosporanici.
EP0785993B1 (de) * 1993-08-06 2003-05-07 Brigham Young University Technology Transfer Office Monoklonale antikörper gegen isozyme der thymidinkinase
AT408660B (de) * 1998-01-28 2002-02-25 Biochemie Gmbh Nukleinsäure-molekül, das für cephalosporin-acetylesterase kodiert
KR100270508B1 (ko) * 1998-04-24 2001-02-01 고두모 신규세파로스포린디아세틸라아제유전자및이를이용한단백질제조방법
US7157418B1 (en) 1998-07-22 2007-01-02 Osprey Pharmaceuticals, Ltd. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
US20030215421A1 (en) * 1999-07-21 2003-11-20 Mcdonald John R. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
US6597946B2 (en) * 1998-11-09 2003-07-22 Transpharma Ltd. Electronic card for transdermal drug delivery and analyte extraction
ES2156812B1 (es) 1999-04-09 2002-02-01 Antibioticos Sau Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.
RU2209244C2 (ru) * 2001-04-28 2003-07-27 Закрытое акционерное общество "АСГЛ-Фармацевтические Инновации" Нуклеиновая кислота, кодирующая лидерную последовательность (варианты), вектор на основе плазмиды р6803-platform (варианты), лидерный пептид (варианты) и способ получения белков
US6727277B1 (en) 2002-11-12 2004-04-27 Kansas State University Research Foundation Compounds affecting cholesterol absorption
ES2272115B1 (es) * 2004-02-13 2008-03-16 Universidad De Murcia Produccion de formas quimericas de cefalosporina c acetilasa.
US7311906B2 (en) 2004-04-30 2007-12-25 Brigham Young University Anti-viral activity of an anti-thymidine kinase monoclonal antibody
US8551486B2 (en) 2004-05-21 2013-10-08 Savoy Pharmaceuticals, Inc. Monoclonal antibodies to human thymidine kinase to treat cancer
US7837998B2 (en) 2004-05-21 2010-11-23 Nathaniel Lallatin Anti-cancer activity of an anti-thymidine kinase monoclonal antibody
US7964378B2 (en) 2005-12-13 2011-06-21 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7723083B2 (en) * 2005-12-13 2010-05-25 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7951566B2 (en) * 2005-12-13 2011-05-31 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US8288136B2 (en) 2005-12-13 2012-10-16 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
EP1960529B1 (de) * 2005-12-13 2010-02-24 E.I. Du Pont De Nemours And Company Herstellung von peraciden unter verwendung eines enzyms mit perhydrolyse-aktivität
US8518675B2 (en) 2005-12-13 2013-08-27 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7473658B2 (en) * 2006-11-13 2009-01-06 E. I. Du Pont Nemours And Company Partially fluorinated amino acid derivatives as gelling and surface active agents
WO2009013611A2 (en) * 2007-07-26 2009-01-29 Orchid Chemicals & Pharmaceuticals Limited Modified esterase and its applications
JP5504268B2 (ja) * 2008-08-13 2014-05-28 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 酵素的過酸生成の制御
US8293221B2 (en) 2008-10-03 2012-10-23 E. I. Du Pont De Nemours And Company Enzymatic peracid generation formulation
US9052304B2 (en) 2009-03-13 2015-06-09 Terrasep, Llc Methods and apparatus for centrifugal liquid chromatography
US20110177148A1 (en) * 2009-07-27 2011-07-21 E. I. Du Pont De Nemours And Company Enzymatic in situ preparation of peracid-based removable antimicrobial coating compositions and methods of use
AU2010281409B2 (en) 2009-07-27 2014-08-14 The Chemours Company Fc, Llc Enzymatic in situ preparation of peracid-based removable antimicrobial coating compositions and methods of use
US8222012B2 (en) * 2009-10-01 2012-07-17 E. I. Du Pont De Nemours And Company Perhydrolase for enzymatic peracid production
US7923233B1 (en) 2009-12-07 2011-04-12 E. I. Du Pont De Nemours And Company Perhydrolase providing improved peracid stability
US7927854B1 (en) 2009-12-07 2011-04-19 E. I. Du Pont De Nemours And Company Perhydrolase providing improved peracid stability
US7960528B1 (en) * 2009-12-07 2011-06-14 E. I. Du Pont De Nemours And Company Perhydrolase providing improved peracid stability
US7910347B1 (en) 2009-12-07 2011-03-22 E. I. Du Pont De Nemours And Company Perhydrolase providing improved peracid stability
US7932072B1 (en) 2009-12-07 2011-04-26 E. I. Du Pont De Nemours And Company Perhydrolase providing improved peracid stability
WO2011082345A2 (en) 2009-12-30 2011-07-07 Brigham Young University Compositions and methods for cancer management using antibodies binding to nucleotide salvage pathway enzymes and complexes thereof
US8206964B2 (en) 2010-03-26 2012-06-26 E. I. Du Pont De Nemours And Company Perhydrolase providing improved specific activity
US8450091B2 (en) * 2010-03-26 2013-05-28 E. I. Du Pont De Nemours And Company Perhydrolase providing improved specific activity
US8445242B2 (en) 2010-03-26 2013-05-21 E. I. Du Pont De Nemours And Company Perhydrolase providing improved specific activity
CN102906256B (zh) 2010-03-26 2014-08-06 纳幕尔杜邦公司 用于纯化蛋白质的方法
WO2011119710A1 (en) 2010-03-26 2011-09-29 E.I. Dupont De Nemours And Company Perhydrolase providing improved specific activity
US8389254B2 (en) * 2010-03-26 2013-03-05 E.I. Du Pont De Nemours And Company Perhydrolase providing improved specific activity
KR20140114269A (ko) * 2010-12-20 2014-09-26 이 아이 듀폰 디 네모아 앤드 캄파니 표적화된 과가수분해효소
US8389257B2 (en) 2010-12-21 2013-03-05 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8394617B2 (en) 2010-12-21 2013-03-12 E.I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8399234B2 (en) 2010-12-21 2013-03-19 E.I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8389258B2 (en) 2010-12-21 2013-03-05 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
WO2012087785A1 (en) 2010-12-21 2012-06-28 E.I. Dupont De Nemours And Company Perhydrolase variant providing improved specific activity
WO2012087789A1 (en) 2010-12-21 2012-06-28 E.I. Dupont De Nemours And Company Perhydrolase variant providing improved specific activity
US8389259B2 (en) 2010-12-21 2013-03-05 E.I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8389256B2 (en) 2010-12-21 2013-03-05 E.I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8962294B2 (en) 2011-10-25 2015-02-24 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8735125B2 (en) 2011-10-25 2014-05-27 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
WO2013062885A1 (en) 2011-10-25 2013-05-02 E.I. Dupont De Nemours And Company Perhydrolase variant providing improved specific activity
US8486679B2 (en) 2011-10-25 2013-07-16 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8546120B2 (en) 2011-10-25 2013-10-01 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8501447B2 (en) 2011-10-25 2013-08-06 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8557556B2 (en) 2011-10-25 2013-10-15 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8956843B2 (en) 2011-10-25 2015-02-17 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
US8809030B2 (en) 2011-10-25 2014-08-19 E. I. Du Pont De Nemours And Company Perhydrolase variant providing improved specific activity
WO2013096045A1 (en) 2011-12-19 2013-06-27 E. I. Du Pont De Nemours And Company Perhydrolase variants providing improved specific activity in the presence of surfactant
BR112014024217B1 (pt) 2012-03-30 2022-04-05 E.I. Du Pont De Nemours And Company Processo de produção de um ácido peroxicarboxílico, composição e sistema de geração e fornecimento de perácido
CN104350153B (zh) 2012-03-30 2018-08-10 纳幕尔杜邦公司 对于过酸的生产有用的酶
JP6574376B2 (ja) 2012-03-30 2019-09-11 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 過酸生成に有用な酵素
JP6335873B2 (ja) 2012-03-30 2018-05-30 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 過酸生成に有用な酵素
CN104364384B (zh) 2012-03-30 2018-12-28 纳幕尔杜邦公司 对于过酸的生产有用的酶
CN111517481A (zh) * 2020-06-18 2020-08-11 山东海景天环保科技股份公司 一种皮革废水深度脱氮用复合碳源及其制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH475284A (de) * 1963-11-19 1969-07-15 Ciba Geigy Verfahren zur Herstellung von Desacetyl-7-amino-cephalosporansäure
JPS49132294A (de) * 1973-04-24 1974-12-18
AT378003B (de) * 1979-07-19 1985-06-10 Glaxo Group Ltd Verfahren zur herstellung von desactylcephalosporin c
US4459405A (en) * 1982-11-16 1984-07-10 Eli Lilly And Company Desacetylcephalosporin sulfones
DE3432060A1 (de) * 1984-08-31 1986-03-06 Bayer Ag, 5090 Leverkusen Immobilisierte zellen von bacillus subtilis, immobilisierungsverfahren und verwendung des praeparats zur abspaltung der 3-acetoxygruppe aus 7-ss-acylamido-cephalosporansaeuren
EP0322032A3 (de) * 1987-12-21 1991-01-30 Merck & Co. Inc. Enzymatische Umwandlung von Cephalosporin C und Derivaten in 7-Aminocephalosporinsäure und Derivate in einem Schritt

Also Published As

Publication number Publication date
KR910018549A (ko) 1991-11-30
GR3018790T3 (en) 1996-04-30
ES2085422T3 (es) 1996-06-01
NZ237878A (en) 1993-02-25
DE69116593D1 (de) 1996-03-07
CN1047202C (zh) 1999-12-08
RU2103364C1 (ru) 1998-01-27
CN1227872A (zh) 1999-09-08
KR960014703B1 (ko) 1996-10-19
IL97915A (en) 2000-08-31
US5338676A (en) 1994-08-16
US5281525A (en) 1994-01-25
AU638124B2 (en) 1993-06-17
DK0454478T3 (da) 1996-02-12
AU7535491A (en) 1991-11-07
EP0454478B1 (de) 1996-01-24
CN1058045A (zh) 1992-01-22
CA2040707A1 (en) 1991-10-28
DE69116593T2 (de) 1996-07-04
CN1155712C (zh) 2004-06-30
CA2040707C (en) 2002-07-09
IL97915A0 (en) 1992-06-21
EP0454478A1 (de) 1991-10-30
JP2651288B2 (ja) 1997-09-10
JPH04228079A (ja) 1992-08-18

Similar Documents

Publication Publication Date Title
ATE133450T1 (de) Cephalosporin acetylhydrolasegen und dadurch kodiertes protein
ATE328069T1 (de) Phosphopantethenyltransferasen und deren verwendungen
US5270176A (en) Method for the selective cleavage of fusion proteins with lysostaphin
ID20467A (id) Mikroorganisme dan proses-proses untuk pembuatan fermentasi l-sistein, l-sistin, n-asetilserin atau turunan-turunan tiazolidina
IL123641A0 (en) Circular dna molecule with conditional origin of replication method for preparing the same and use thereof in gene therapy
PT673427E (pt) Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao
ATE137535T1 (de) Enzymatisches verfahren zur herstellung von 7- aminocephalosporinsäure und derivate
DK0475652T3 (da) Cephalosporin C-acylase
HUP9904162A2 (hu) Mutáns G-penicillin-acilázok
EP0842937A3 (de) Verfahren zur Herstellung der Cephalosporinderivate Cefotaxime und Ceftriaxone
CA2085292A1 (en) Enzyme of Use in Chitosan Hydrolysis
KR940005634A (ko) 세팔로스포란 환의 7-아미노 그룹의 아실화 방법
CA2100987A1 (en) A transformant capable of producing d-amino acid oxidase
CA2204743A1 (en) D-pantolactone hydrolase and gene encoding the same
AU6960291A (en) Method for the synthesis of semi-synthetic antibiotics in thermodynamically controlled water-cosolvent organic miscible apolar systems by using penicillin g acylase
SE8803124L (sv) Glasmikropaerlor med bakteriostatiska egenskaper och foerfarande foer framstaellning av saadana mikropaerlor
AU4846196A (en) Process for the recovery of cephalexin
TR199901164A2 (xx) Betalaktam antibiyotiklerin enzimatik �retim prosesi.
CN106119233B (zh) 头孢菌素酰化酶突变体及其编码基因与应用
ZA988754B (en) An enzymatic process for preparing 7beta- (4-carboxy-butanamido) cephalosporanic acid using the modified D-amino acid oxydase enzyme from trigonopsis variabilis produced in escherichia coli
HUP0301202A2 (hu) Glutaril-cefalosporin amidáz Pseudomonas diminuta BS-203-ból
EP0149915A3 (de) Stärkeverwendung durch gram-negative Mikroorganismen
Kawata et al. Characterization of d-Alanyl-(d)-meso-2, 6-diaminopimelic Acid Endopeptidase from Streptomyces globisporus 1829
TH10372EX (th) จีนเซฟาโลสปอรินแอเซทิลไฮโดรเลสและโปรตีนที่ประมวลรหัสไว้ในจีนดังกล่าว
BG51517A1 (en) Cephem derivatives of 5-carboxy-2-benzoxazolon and a method for their production

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time