AR103586A1 - Proteínas quiméricas que mejoran la actividad de los dominios de unión a adn y factores de transcripción en plantas - Google Patents

Proteínas quiméricas que mejoran la actividad de los dominios de unión a adn y factores de transcripción en plantas

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Publication number
AR103586A1
AR103586A1 ARP150104128A ARP150104128A AR103586A1 AR 103586 A1 AR103586 A1 AR 103586A1 AR P150104128 A ARP150104128 A AR P150104128A AR P150104128 A ARP150104128 A AR P150104128A AR 103586 A1 AR103586 A1 AR 103586A1
Authority
AR
Argentina
Prior art keywords
seq
gif
activity
molecular fraction
dbd
Prior art date
Application number
ARP150104128A
Other languages
English (en)
Inventor
Schommer Carla
Esteban Rodriguez Virasoro Ramiro
Manuel Debernardi Juan
Palatnik Javier
Original Assignee
Consejo Nac De Investig Científicas Y Técnicas (Conicet)
Univ Nac De Rosario
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nac De Investig Científicas Y Técnicas (Conicet), Univ Nac De Rosario filed Critical Consejo Nac De Investig Científicas Y Técnicas (Conicet)
Publication of AR103586A1 publication Critical patent/AR103586A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Método de potenciación de la actividad de un factor de transcripción en una planta que comprende expresar en dicha planta una proteína quimérica que comprende una fracción molecular GIF que comprende un dominio de GIF o un fragmento del mismo y una fracción molecular dominio de unión a ADN (DBD). Las proteínas quiméricas comprenden una fracción molecular GIF que comprende un dominio de GIF o fragmento del mismo, o un polipéptido con una secuencia de aminoácidos que tiene al menos 85%, preferiblemente al menos 90%, aún más preferiblemente al menos 95% de identidad de secuencia con la secuencia de aminoácidos de un dominio de GIF o fragmento del mismo; y una fracción molecular DBD que comprende DBD o factor de transcripción vegetal cuya potenciación de la actividad se pretende. o una proteína de unión que es capaz de formar un complejo terciario con el DBD o factor de transcripción cuya modificación y/o potenciación de la actividad se pretende. También se refiere a polinucleótidos y polipéptidos útiles en el método de la presente. Reivindicación 6: El método de la reivindicación 5, en donde la fracción molecular GIF comprende o consiste en un polipéptido seleccionado de GlF1D1 (SEQ ID Nº 3), GIF1D2 (SEQ ID Nº 4), GIF1D3 (SEQ ID Nº 5), GIF1D4 (SEQ ID Nº 6), GIF1D5 (SEQ ID Nº 7), GlF1D6 (SEQ ID Nº 8), GIF1D7 (SEQ ID Nº 9), y GIF1D10 (SEQ ID Nº 61).
ARP150104128A 2014-12-17 2015-12-17 Proteínas quiméricas que mejoran la actividad de los dominios de unión a adn y factores de transcripción en plantas AR103586A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462093167P 2014-12-17 2014-12-17

Publications (1)

Publication Number Publication Date
AR103586A1 true AR103586A1 (es) 2017-05-24

Family

ID=55229761

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP150104128A AR103586A1 (es) 2014-12-17 2015-12-17 Proteínas quiméricas que mejoran la actividad de los dominios de unión a adn y factores de transcripción en plantas

Country Status (3)

Country Link
US (1) US10822612B2 (es)
AR (1) AR103586A1 (es)
WO (1) WO2016098027A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2020008580A (es) * 2018-02-14 2020-09-21 Inst Genetics & Developmental Biology Cas Metodos para aumentar la eficiencia en el uso de nutrientes.
CN111662922A (zh) * 2019-03-05 2020-09-15 江苏师范大学 拟南芥ant基因在提高鸡冠花耐旱性和种子产量中的应用
WO2021007284A2 (en) * 2019-07-11 2021-01-14 The Regents Of The University Of California Methods for improved regeneration of transgenic plants using growth-regulating factor (grf), grf-interacting factor (gif), or chimeric grf-gif genes and proteins
CN114317573B (zh) * 2022-01-17 2024-02-09 南京农业大学 一种提高森林草莓遗传转化效率的嵌合基因及其载体和应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2537940A3 (en) * 2007-09-14 2013-04-10 BASF Plant Science GmbH Plants having increased yield-related traits and a method for making the same
CA2833225A1 (en) * 2011-05-09 2012-11-15 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
GB201200075D0 (en) 2012-01-04 2012-02-15 Univ Nac De Rosario Grf3 mutants, methods and plants

Also Published As

Publication number Publication date
WO2016098027A1 (en) 2016-06-23
US20170362601A1 (en) 2017-12-21
US10822612B2 (en) 2020-11-03

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