AR085253A1 - Metodo para producir una clorofilasa - Google Patents

Metodo para producir una clorofilasa

Info

Publication number
AR085253A1
AR085253A1 ARP120100518A ARP120100518A AR085253A1 AR 085253 A1 AR085253 A1 AR 085253A1 AR P120100518 A ARP120100518 A AR P120100518A AR P120100518 A ARP120100518 A AR P120100518A AR 085253 A1 AR085253 A1 AR 085253A1
Authority
AR
Argentina
Prior art keywords
recombinant enzyme
host cell
chlorophyll
chlorophylase
produce
Prior art date
Application number
ARP120100518A
Other languages
English (en)
Inventor
Susan Mampusti Madrid
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of AR085253A1 publication Critical patent/AR085253A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01014—Chlorophyllase (3.1.1.14)

Abstract

Método para producir una enzima recombinante que puede hidrolizar la clorofila o un derivado de clorofila, que comprende un paso en el que se expresa la enzima recombinante en el interior de una célula huésped eucariota. En una forma de realización la célula huésped es una célula fúngica tal como Trichoderma.Reivindicación 6: Un método de acuerdo con cualquiera de las reivindicaciones precedentes, caracterizado porque la enzima recombinante deriva de Arabidopsis thaliana, de Brassica oleracea, de Ricinus communis, de Ginkgo biloba, de Populus trichocarpa, de Vitis vinifera, de Phyllostachys heterocicla, de Sorghum bicolor, de Glycine max, de Pachira macrocarpa, de Triticum aestivum, de Citrus sinensis, de Zea mays, de Chenopodium album, de Picea sitchensis o de Chiamydomonas reinhardtii.
ARP120100518A 2011-02-23 2012-02-16 Metodo para producir una clorofilasa AR085253A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161445657P 2011-02-23 2011-02-23

Publications (1)

Publication Number Publication Date
AR085253A1 true AR085253A1 (es) 2013-09-18

Family

ID=45815921

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120100518A AR085253A1 (es) 2011-02-23 2012-02-16 Metodo para producir una clorofilasa

Country Status (6)

Country Link
US (1) US20140051146A1 (es)
EP (1) EP2678427A2 (es)
CN (1) CN103797114A (es)
AR (1) AR085253A1 (es)
CA (1) CA2825628A1 (es)
WO (1) WO2012114231A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014086928A1 (en) * 2012-12-05 2014-06-12 Novozymes A/S Polypeptides having chlorophyllase activity and polynucleotides encoding same

Family Cites Families (26)

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US4601986A (en) 1983-07-29 1986-07-22 Phillips Petroleum Company Protein product of reduced nucleic acid content and low allergenicity
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5801034A (en) 1989-06-13 1998-09-01 Genencor International Method for killing cells without lysis and enzyme recovery
DE4112440C1 (es) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
DK0765394T3 (da) 1994-06-03 2001-12-10 Novozymes As Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
JP4053595B2 (ja) 1995-09-22 2008-02-27 メディカル・リサーチ・カウンシル 核酸の突然変異誘発におけるまたはそれに関する改良
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6344328B1 (en) 1995-12-07 2002-02-05 Diversa Corporation Method for screening for enzyme activity
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
MXPA00011569A (es) 1999-03-26 2004-12-03 Diversa Corp Re-ensamble de acidos nucleicos mediado por exonucleasa en evolucion dirigida.
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
EP1307548A2 (en) 2000-07-13 2003-05-07 Maxygen, Inc. Novel lipase genes
AU2001296577A1 (en) * 2000-10-05 2002-04-15 E.I. Du Pont De Nemours And Company Chlorophyllases
AU2003298577A1 (en) 2002-09-10 2004-05-04 Genencor International, Inc. Induction of gene expression using a high concentration sugar mixture
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
WO2005121333A1 (en) 2004-06-14 2005-12-22 Novozymes A/S Signal peptide for producing a polypeptide
EP2468853B1 (en) * 2004-06-16 2015-04-01 DSM IP Assets B.V. Method for enzymatic decolorization of pheophytin
MX341624B (es) * 2007-04-09 2016-08-26 Evogene Ltd Polinucleotidos, polipeptidos y metodos para aumentar el contenido de aceite, la velocidad de crecimiento y biomasa de las plantas.
AR077042A1 (es) * 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina

Also Published As

Publication number Publication date
WO2012114231A3 (en) 2013-04-04
WO2012114231A2 (en) 2012-08-30
EP2678427A2 (en) 2014-01-01
US20140051146A1 (en) 2014-02-20
CA2825628A1 (en) 2012-08-30
CN103797114A (zh) 2014-05-14

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