AR033547A1 - Polinucleotido aislado, construccion de acido nucleico, celula huesped, planta transgenica de brassica, soja y maiz, semilla y progenie de la misma, metodo para modificar el contenido de acidos grasos saturados en la celula huesped, metodo para incrementar la expresion de beta-cetoacil-acp sintetasa - Google Patents

Polinucleotido aislado, construccion de acido nucleico, celula huesped, planta transgenica de brassica, soja y maiz, semilla y progenie de la misma, metodo para modificar el contenido de acidos grasos saturados en la celula huesped, metodo para incrementar la expresion de beta-cetoacil-acp sintetasa

Info

Publication number
AR033547A1
AR033547A1 ARP010103555A ARP010103555A AR033547A1 AR 033547 A1 AR033547 A1 AR 033547A1 AR P010103555 A ARP010103555 A AR P010103555A AR P010103555 A ARP010103555 A AR P010103555A AR 033547 A1 AR033547 A1 AR 033547A1
Authority
AR
Argentina
Prior art keywords
cellular
fatty acids
saturated fatty
sequence
brassica
Prior art date
Application number
ARP010103555A
Other languages
English (en)
Inventor
Katayoon Dehesh
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of AR033547A1 publication Critical patent/AR033547A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Secuencia de polinucleotidos idéntica en su secuencia a la secuencia identificada como SEQ ID Ns1, secuencia que sean 50-99% idénticas a la misma, secuencias complementarias, secuencias que hibridizan bajo condiciones de hibridizacion estricta con las mencionadas secuencias y construcciones que comprenden dicha secuencia. Dichas secuencias de polinucleotidos que se pueden obtener tanto de fuentes naturales, tales como las cianobacterias, como artificiales, codifican polipéptidos en particular la beta-cetoacil- ACP sintetasa (KAS), que cumple un rol indispensable en la síntesis de ácidos grasos. Método para modificar y reducir el contenido de ácidos grasos saturados en una célula huésped de una planta transgénica de soja, maíz, o Brassica. Las células huésped, semillas y progenies de dichas plantas así como también los aceites con ácidos grasos saturados reducidos que de ellas se obtienen también se describen.
ARP010103555A 2000-07-25 2001-07-26 Polinucleotido aislado, construccion de acido nucleico, celula huesped, planta transgenica de brassica, soja y maiz, semilla y progenie de la misma, metodo para modificar el contenido de acidos grasos saturados en la celula huesped, metodo para incrementar la expresion de beta-cetoacil-acp sintetasa AR033547A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22070200P 2000-07-25 2000-07-25

Publications (1)

Publication Number Publication Date
AR033547A1 true AR033547A1 (es) 2003-12-26

Family

ID=22824596

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010103555A AR033547A1 (es) 2000-07-25 2001-07-26 Polinucleotido aislado, construccion de acido nucleico, celula huesped, planta transgenica de brassica, soja y maiz, semilla y progenie de la misma, metodo para modificar el contenido de acidos grasos saturados en la celula huesped, metodo para incrementar la expresion de beta-cetoacil-acp sintetasa

Country Status (7)

Country Link
US (2) US6706950B2 (es)
EP (1) EP1303616A2 (es)
AR (1) AR033547A1 (es)
AU (1) AU2001279007A1 (es)
BR (1) BR0112716A (es)
CA (1) CA2412400A1 (es)
WO (1) WO2002008403A2 (es)

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Also Published As

Publication number Publication date
US6706950B2 (en) 2004-03-16
EP1303616A2 (en) 2003-04-23
US7371924B2 (en) 2008-05-13
US20020059661A1 (en) 2002-05-16
WO2002008403A3 (en) 2003-01-16
AU2001279007A1 (en) 2002-02-05
US20040132189A1 (en) 2004-07-08
BR0112716A (pt) 2003-06-24
CA2412400A1 (en) 2002-01-31
WO2002008403A2 (en) 2002-01-31

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