AR020090A1 - Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion - Google Patents

Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion

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Publication number
AR020090A1
AR020090A1 ARP990102815A ARP990102815A AR020090A1 AR 020090 A1 AR020090 A1 AR 020090A1 AR P990102815 A ARP990102815 A AR P990102815A AR P990102815 A ARP990102815 A AR P990102815A AR 020090 A1 AR020090 A1 AR 020090A1
Authority
AR
Argentina
Prior art keywords
plant
polypeptide
cell
expression
seed
Prior art date
Application number
ARP990102815A
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English (en)
Original Assignee
John Innes Ct
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by John Innes Ct filed Critical John Innes Ct
Publication of AR020090A1 publication Critical patent/AR020090A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Célula vegetal monocotiledonea o semilla de ella que contiene un polipéptido de mamífero, producido por la expresion, dentro de la célula o de la semilla,a paartir de un cassette de expresion, que comprende una secuencia de codificacion para el polipéptido , de modo tal que en la célula vegetal o la semilla hayaun rasgo adicional seleccinado del grupo formado por: (a) el polipéptido que se expresa fusionado a una senal de retencion del retículo endoplasmático (RE);(b) la secuencia de codificacion para el polipéptido que está precedida, en el cassette de expresion, por una secuencia líder 5 no traducida (5 UTR); (c) elpolipéptido que se expresa fusionado al péptido líder; (d) cualquier combinacion de un par formado entre (a),(b) y (c); y (e)una combinacion de (a), (b) y(c). Planta o parte de una planta, método para hacer una célula vegetal monocotiledonea, método para hacer una planta, uso de una construccion de expresionpara producir una célula de planta o semilla monocotiledonea. Usode una construccion de expresion para producir una planta transgénica. Método para producirplantas, células o semillas monocotiledoneas, por ejemplo, arroz o trigo, con un polipéptido de mamífero, como ser un anticuerpo o fragmento del mismo,producido por la expresion dentro de la célula o semilla de un casete de expresion que comprende una secuencia codificante para el polipéptido. Para aumentarel rendimiento del polipéptido dentro de la célula o semillas, la secuencia codificante puede fusionarse a secuencias adicionales de modo tal que elpolipéptido se fusiona a una senal de retencion ER, tal como, KDEL o HDEL,y/o a un péptido líder,tal como,una senal para dirigir un objetivo a los cloroplastosTambién para aumentar el rendimiento, la secuencia codificante para el polipéptido puede ser fusionada a secuencias adicionales, por ejemplo, a una secuencialíder no traducida 5. La célula o semilla puede contener uno o más casetes de expresion donde cada uno codifica una parte del polipéptido, por ejemplo, unacadena de un polipéptido multi-cadena. Se refiere a planta, célula o semilla transgénica obtenible por los métodos descriptos y a casetes de expresion para
ARP990102815A 1998-06-15 1999-06-11 Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion AR020090A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8932298P 1998-06-15 1998-06-15

Publications (1)

Publication Number Publication Date
AR020090A1 true AR020090A1 (es) 2002-04-10

Family

ID=22217017

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990102815A AR020090A1 (es) 1998-06-15 1999-06-11 Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion

Country Status (8)

Country Link
US (2) US20020078472A1 (es)
EP (1) EP1088061A4 (es)
AR (1) AR020090A1 (es)
AU (1) AU4571699A (es)
BR (1) BR9911270A (es)
CA (1) CA2330933A1 (es)
MX (1) MXPA00012520A (es)
WO (1) WO1999066026A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1123398B1 (en) * 1998-10-16 2005-08-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Molecular pathogenicide mediated plant disease resistance
US20020174453A1 (en) * 2001-04-18 2002-11-21 Henry Daniell Production of antibodies in transgenic plastids
EP1268832A4 (en) * 2000-02-29 2003-04-02 Univ Auburn PRODUCTION OF ANTIBODIES IN TRANSGENIC PLASTS
EP1263281B1 (en) * 2000-02-29 2012-04-11 Auburn University Multiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
US20030041353A1 (en) 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
US7304208B2 (en) * 2000-05-02 2007-12-04 Ventria Bioscience Expression of human serum albumin (HSA) in monocot seeds
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
US6472176B2 (en) * 2000-12-14 2002-10-29 Genvec, Inc. Polynucleotide encoding chimeric protein and related vector, cell, and method of expression thereof
JP2005525081A (ja) 2001-06-05 2005-08-25 カレン ケイ． オオイシ 植物からの生物活性ミュラー管抑制物質を含むＴＧＦ−βタンパク質の遺伝子発現および産生法
US8558058B2 (en) * 2001-12-06 2013-10-15 Applied Biotechnology Institute Monocotyledonous seed expressing exo-1,4B-glucanase
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
JP2007517504A (ja) * 2003-12-09 2007-07-05 ベントリア バイオサイエンス 融合担体として種子貯蔵蛋白を利用する植物種子中の融合ポリペプチドの高水準発現
JP2008507258A (ja) * 2004-04-22 2008-03-13 フラウンホーファー・ゲゼルシャフト ツーァ フェルデルンク デァ アンゲヴァンテン フォルシュンク エー ファウ 連鎖球菌表面抗原ｉ／ｉｉに特異的に結合する二重特異性抗体および前記の使用の方法
DE602005021379D1 (de) 2004-10-13 2010-07-01 Protalix Ltd System und verfahren zur produktion von antikörpern in der pflanzenzellkultur
WO2006066340A1 (en) * 2004-12-21 2006-06-29 Grain Biotech Australia Pty Ltd A method for the high expression of immunoglobulin in plants
US20100047164A1 (en) * 2008-04-11 2010-02-25 Duke University Anti-Tumor Antibodies
US10308948B2 (en) 2011-07-27 2019-06-04 Applied Biotechnology Institute, Inc. Method of increasing expression of nucleic acid molecules in plants using multiple transcription units
WO2021178388A1 (en) * 2020-03-02 2021-09-10 Ventria Bioscience Inc. Enhanced expression yield of immunoglobulin a in eukaryotes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693506A (en) * 1993-11-16 1997-12-02 The Regents Of The University Of California Process for protein production in plants
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
WO1998010062A1 (en) * 1996-09-03 1998-03-12 Monsanto Company Monocot seed gene expression system

Also Published As

Publication number Publication date
MXPA00012520A (es) 2002-05-08
US20020078472A1 (en) 2002-06-20
WO1999066026A2 (en) 1999-12-23
EP1088061A2 (en) 2001-04-04
CA2330933A1 (en) 1999-12-23
WO1999066026A3 (en) 2000-01-27
BR9911270A (pt) 2001-03-13
EP1088061A4 (en) 2005-03-16
AU4571699A (en) 2000-01-05
US20030051275A1 (en) 2003-03-13

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