ZA962836B - Method of reducing the formation of artifacts. - Google Patents

Method of reducing the formation of artifacts.

Info

Publication number
ZA962836B
ZA962836B ZA9602836A ZA962836A ZA962836B ZA 962836 B ZA962836 B ZA 962836B ZA 9602836 A ZA9602836 A ZA 9602836A ZA 962836 A ZA962836 A ZA 962836A ZA 962836 B ZA962836 B ZA 962836B
Authority
ZA
South Africa
Prior art keywords
pct
artifacts
formation
reducing
transcribing
Prior art date
Application number
ZA9602836A
Other languages
English (en)
Inventor
Peter Bauer
Arndt Dr Rolfs
Vera Dr Regit-Zagrosek
Eckart Prof Dr Fleck
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of ZA962836B publication Critical patent/ZA962836B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Pyrane Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Lighting Device Outwards From Vehicle And Optical Signal (AREA)
ZA9602836A 1995-04-11 1996-04-10 Method of reducing the formation of artifacts. ZA962836B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19513728A DE19513728A1 (de) 1995-04-11 1995-04-11 Verfahren zur Reduzierung der Bildung von Artefakten

Publications (1)

Publication Number Publication Date
ZA962836B true ZA962836B (en) 1997-10-10

Family

ID=7759479

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA9602836A ZA962836B (en) 1995-04-11 1996-04-10 Method of reducing the formation of artifacts.

Country Status (10)

Country Link
US (1) US6124093A (fr)
EP (1) EP0871769B1 (fr)
JP (1) JP4126348B2 (fr)
AT (1) ATE205544T1 (fr)
AU (1) AU5335596A (fr)
DE (2) DE19513728A1 (fr)
ES (1) ES2162044T3 (fr)
IL (1) IL117880A0 (fr)
WO (1) WO1996032501A1 (fr)
ZA (1) ZA962836B (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5798257A (en) * 1990-07-09 1998-08-25 Research Corporation Technologies, Inc. Nucleic acid encoding human MTS-1 protein
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
WO1994012657A1 (fr) * 1992-11-20 1994-06-09 Katcher Harold L Methodes de pcr par adjonction directe de phenol, de transcription inverse (tr) et de pcr-tr
DK0632134T3 (da) * 1993-07-01 2001-12-03 Hoffmann La Roche Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner

Also Published As

Publication number Publication date
EP0871769A1 (fr) 1998-10-21
DE19513728A1 (de) 1996-10-31
JP4126348B2 (ja) 2008-07-30
JPH11503325A (ja) 1999-03-26
DE59607694D1 (de) 2001-10-18
ES2162044T3 (es) 2001-12-16
US6124093A (en) 2000-09-26
WO1996032501A1 (fr) 1996-10-17
EP0871769B1 (fr) 2001-09-12
ATE205544T1 (de) 2001-09-15
AU5335596A (en) 1996-10-30
IL117880A0 (en) 1996-12-05

Similar Documents

Publication Publication Date Title
WO1995006731A3 (fr) Acide nucleique enzymatique contenant un non-nucleotide
EP1700915A3 (fr) Molécules d'ADN enzymatiques
PL337447A1 (en) Lactic acid purifying method
ATE324464T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
EP0808910A3 (fr) Dispositif et méthode de production et d'utilisation d'ions Fe(III) provenant de bactéries
BR9714569B1 (pt) processo e dispositivo de cultura para registrar e detectar ou contar microorganismos em microvolumes.
AU1272000A (en) Method for the quantitative detection of nucleic acids
CA2239005A1 (fr) Production d'acrylate d'ammonium
EP0666320A3 (fr) Procédé de préparation d'acides alpha hydroxyliques ou d'amides alpha hydroxyliques par des microorganismes.
GB0002261D0 (en) Fluorescent detection method & reagent
GB2346695A (en) Fluorometric method for monitoring and detecting nucleic acid amplification
ATE486949T1 (de) Verfahren zur herstellung von n- acetylneuraminsäure
DE3882078D1 (de) Amperometrische methode zur titration von nadh (1,4-dihydronicotinamid-adenine-dinucleotide) in loesung.
AU696819B2 (en) Biological substance for the physico-chemical stabilisation of wines
ZA962836B (en) Method of reducing the formation of artifacts.
EP1227155A4 (fr) Proc d de production d'ubiquinone-10
ES2160829T3 (es) Procedimiento para la obtencion de riboflavina por medio de microorganismos con actividad de isocitratoliasa modificada.
WO2000034504A3 (fr) Procede d'extraction de proteines
WO2000065632A3 (fr) Procede de decontamination de la levure
ES2099678A1 (es) Procedimiento para la inactivacion de virus en proteinas.
SE9403805D0 (sv) Method of preparing oligonucleotide probes or primers, vector therefor and use thereof
AU4944996A (en) Dna fragment encoding d-amino acid oxidase
ATE289067T1 (de) Verfahren und mittel zur bestimmung von jodid
FI964208A (fi) Menetelmä metallofosfaatin saostumisen kontrolloimiseksi fermentoinneissa, joissa solutiheys on suuri
AU2620395A (en) Shuttle vectors for the introduction of dna into mycobacteria and utilization of such bacteria as vaccines