ZA200003401B - L-lysine-producing corynebacteria and process for the preparation of lysine. - Google Patents

L-lysine-producing corynebacteria and process for the preparation of lysine.

Info

Publication number
ZA200003401B
ZA200003401B ZA200003401A ZA200003401A ZA200003401B ZA 200003401 B ZA200003401 B ZA 200003401B ZA 200003401 A ZA200003401 A ZA 200003401A ZA 200003401 A ZA200003401 A ZA 200003401A ZA 200003401 B ZA200003401 B ZA 200003401B
Authority
ZA
South Africa
Prior art keywords
lysine
amplified
gene
preparation
dapa
Prior art date
Application number
ZA200003401A
Inventor
Carolina Kreutzer
Bettina Moeckel
Walter Pfefferle
Lothar Eggeling
Hermann Sahm
Miroslav Patek
Original Assignee
Degussa
Forschungszentrum Juelich Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa, Forschungszentrum Juelich Gmbh filed Critical Degussa
Publication of ZA200003401B publication Critical patent/ZA200003401B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

New L-lysine producing coryneform bacteria (A) contain amplified pyc (pyruvate carboxylase) and dapA (dihydrodipicolinate) genes, where the promoter of the dapA gene contains the mutation MC20 or MA16 (as shown in respective sequences of 79 or 80 bases given in the specification as SEQ-ID-NO: 5 and SEQ-ID-NO: 6). (A) optionally also contains amplified lycC, lysE and/or dapB gene(s). An independent claim is included for the preparation of L-lysine by fermentation of coryneform bacteria having an amplified pyc gene, where the bacteria additionally contain the amplified dapA gene as specified for (A).
ZA200003401A 1999-07-07 2000-07-06 L-lysine-producing corynebacteria and process for the preparation of lysine. ZA200003401B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19931314A DE19931314A1 (en) 1999-07-07 1999-07-07 L-lysine producing coryneform bacteria and method for producing lysine

Publications (1)

Publication Number Publication Date
ZA200003401B true ZA200003401B (en) 2001-02-02

Family

ID=7913923

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200003401A ZA200003401B (en) 1999-07-07 2000-07-06 L-lysine-producing corynebacteria and process for the preparation of lysine.

Country Status (15)

Country Link
EP (2) EP1067193B8 (en)
JP (1) JP2001037495A (en)
KR (1) KR100782867B1 (en)
CN (2) CN1231574C (en)
AT (2) ATE307210T1 (en)
AU (1) AU3938600A (en)
BR (1) BR0002445A (en)
CA (1) CA2310890A1 (en)
DE (3) DE19931314A1 (en)
ES (1) ES2247987T3 (en)
HU (1) HUP0002584A2 (en)
ID (1) ID26461A (en)
MX (1) MXPA00006491A (en)
SK (1) SK10202000A3 (en)
ZA (1) ZA200003401B (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10021829A1 (en) * 2000-05-04 2001-11-08 Degussa New nucleotide sequences coding for the pgsA2 gene
JP4120364B2 (en) 2002-11-20 2008-07-16 味の素株式会社 Method for producing L-lysine or L-arginine using methanol-assimilating bacteria
DE10344739A1 (en) * 2003-09-26 2005-04-14 Degussa Ag Process for the fermentative production of L-amino acids using coryneform bacteria
CN103146593B (en) * 2004-01-30 2015-07-15 味之素株式会社 L-amino acid-producing microorganism and method for producing l-amino acid
CN101573438B (en) * 2006-09-15 2013-01-02 Cj第一制糖株式会社 A corynebacteria having enhanced L-lysine productivity and a method of producing L-lysine using the same
KR100789274B1 (en) * 2007-01-15 2008-01-02 씨제이 주식회사 Novel promoter nucleic acid molecule derived from Corynebacterium glutamicum recombinant vector comprising the promoter host cell comprising the recombinant vector and method of expressing a gene using the host cell
KR100830826B1 (en) 2007-01-24 2008-05-19 씨제이제일제당 (주) Process for producing fermentation product from carbon sources containing glycerol using corynebacteria
JP2010088301A (en) 2007-02-01 2010-04-22 Ajinomoto Co Inc Method for production of l-amino acid
KR101126041B1 (en) 2008-04-10 2012-03-19 씨제이제일제당 (주) A transformation vector using transposon, a microorganism transformed with the vector and method of producing l-lysine using the microorganism
US8932861B2 (en) 2008-04-10 2015-01-13 Cj Cheiljedang Corporation Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism
DE102010019059A1 (en) * 2010-05-03 2011-11-03 Forschungszentrum Jülich GmbH Sensors for intracellular metabolite detection
WO2011158975A1 (en) * 2010-06-15 2011-12-22 Paik Kwang Industrial Co., Ltd. Production process for amino acids of the aspartate family using microorganisms
CN102318739B (en) * 2011-06-08 2012-08-15 宁夏伊品生物科技股份有限公司 Three-level fermentation of lysine and coating products thereof
CN103215286B (en) * 2012-11-12 2015-11-25 江南大学 For the recombinant DNA of fermentation production of L-lysine, bacterial strain and application thereof
CN106635944A (en) * 2016-12-29 2017-05-10 廊坊梅花生物技术开发有限公司 Glutamic acid corynebacterium and construction method and application thereof
DE102017004751A1 (en) * 2017-05-18 2018-11-22 Forschungszentrum Jülich GmbH Pyruvate carboxylase and DNA encoding the pyruvate carboxylase, plasmid containing the DNA, and microorganism for production, and methods for producing products whose biosynthesis involves oxaloacetate as a precursor and chromosome
WO2020051420A1 (en) 2018-09-07 2020-03-12 Archer Daniels Midland Company Engineered strains of corynebacteria
CN110218749B (en) * 2019-05-16 2023-05-12 内蒙古伊品生物科技有限公司 Method for producing lysine by fermentation using NCgl 1859-modified bacteria
WO2020257395A1 (en) * 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
CN111850010B (en) * 2020-06-08 2021-04-09 黑龙江伊品生物科技有限公司 dapB gene modified recombinant strain and construction method and application thereof
CN115449519B (en) * 2021-06-08 2023-04-07 中国科学院天津工业生物技术研究所 Polynucleotide having promoter activity based on dapB gene and use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3943117A1 (en) * 1989-12-27 1991-07-04 Forschungszentrum Juelich Gmbh METHOD FOR THE FERMENTATIVE PRODUCTION OF AMINO ACID, IN PARTICULAR L-LYSINE, THEREFORE SUITABLE MICROORGANISMS AND RECOMBINANT DNA
HU219600B (en) * 1993-08-24 2001-05-28 Ajinomoto Co., Inc. Variant phosphoenolpyruvate carboxylase, gene thereof, and process for producing amino acid
US20020086370A1 (en) * 1995-06-07 2002-07-04 Seiko Otsuna Method of producing l-lysine
DE19548222A1 (en) * 1995-12-22 1997-06-26 Forschungszentrum Juelich Gmbh Process for the microbial production of amino acids through increased activity of export carriers
SK285201B6 (en) * 1996-12-05 2006-08-03 Ajinomoto Co., Inc. Recombinant DNA autonomously replicable in cells of coryneform bacteria, coryneform bacterium and method for producing L-lysine, vector pVK7
DE19831609B4 (en) * 1997-10-04 2009-11-12 Evonik Degussa Gmbh Process for the preparation of amino acids of the aspartate and / or glutamate family and agents which can be used in the process

Also Published As

Publication number Publication date
CN1847389A (en) 2006-10-18
DE19931314A1 (en) 2001-01-11
CN1280185A (en) 2001-01-17
EP1067193B1 (en) 2005-10-19
AU3938600A (en) 2001-04-12
CA2310890A1 (en) 2001-01-07
EP1067193B8 (en) 2006-02-01
KR20010049728A (en) 2001-06-15
EP1619252B1 (en) 2007-06-27
BR0002445A (en) 2001-05-08
CN1231574C (en) 2005-12-14
SK10202000A3 (en) 2001-09-11
EP1619252A1 (en) 2006-01-25
ID26461A (en) 2001-01-11
ES2247987T3 (en) 2006-03-16
HU0002584D0 (en) 2000-09-28
JP2001037495A (en) 2001-02-13
CN100451104C (en) 2009-01-14
DE50014448D1 (en) 2007-08-09
MXPA00006491A (en) 2002-06-04
ATE307210T1 (en) 2005-11-15
HUP0002584A2 (en) 2002-09-28
KR100782867B1 (en) 2007-12-06
EP1067193A1 (en) 2001-01-10
ATE365806T1 (en) 2007-07-15
DE50011356D1 (en) 2006-03-02

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