WO2024116027A1

WO2024116027A1 - Hair care composition, a formulation and method of treating and managing hair

Info

Publication number: WO2024116027A1
Application number: PCT/IB2023/061790
Authority: WO
Inventors: Rahul Yadav; Karan MITTAL; Neetesh Kumar AGRAWAL; Vaibhav KHARE; Sampada KHARE
Original assignee: Uprising Science Private Limited
Priority date: 2022-11-28
Filing date: 2023-11-22
Publication date: 2024-06-06

Abstract

The present disclosure relates to hair care composition having excellent hair care effect. The composition comprises monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids. The present disclosure also relates to a formulation comprising the composition. The composition and the formulation of the present disclosure affects and/or works on multiple targets leading to disulphide bond formation, amide bond formation and boosting the level of amino acids in hair shaft, simultaneously.

Description

“HAIR CARE COMPOSITION, A FORMULATION AND METHOD OF TREATING AND

MANAGING HAIR”

TECHNICAL FIELD

The present disclosure relates to field of cosmetics. The present disclosure particularly relates to a composition having excellent hair care effect. The composition provides for effective management and treatment of hair. The disclosure relates to method of preparing the composition and method of managing and treating hair.

BACKGROUND OF THE DISCLOSURE

Generally, human hair is composed of three layers, cuticle, the cortex and the medulla. The cuticle layer is the outermost layers and has the functions of coating and protecting cortex, and undamaged cuticle layer of the healthy hair has smooth surface and the functions of refracting light and reducing friction among hairs.

Hair is susceptible to external environmental influences, in particular, hair damage due to dry cold wind, indoor heating, excessive combing stress, malnutrition and insufficient sleep. In addition, currently, people regard hair as a fashion, and the hair is frequently subjected to numerous hair treatment, such as dyeing, perming, bleaching, excessive use of hair dressing products, and increased use of hair dryers for long time, which is known to frequently cause hair damage.

When hair is damaged by various physical, chemical or environmental factors, cuticle damage of hair and loss of protein and lipid in the hair is caused.

Further, when cosmetic compositions are applied to hair shaft to improve hair appearance by providing cleansing, conditioning, lustre and strength to the hair fibre, it is noted that integrity of hair fibre is affected by multiple mechanisms, such as altering hydrogen bonds and salt bridges, hydrolyzing amide bonds and rupturing the disulfide bonds present in the hair shaft. Damage of hair due to destruction in different types of bonds in the hair fibre tends to lose its strength from any point between the root to tip. Furthermore, the cosmetic composition and techniques/methods available for treatment of damaged hair or for managing hair, work through single mechanism and lack the comprehensive approach while managing and treating the hair shaft, such as amide bond and disulphide bond repair simultaneously.

Thus, there is a need for a hair care composition that can provide comprehensive approach while managing and treating hair and that can address the limitations noted in the existing hair care composition and treatments.

The present disclosure provides a composition that solves the problem noted in the existing hair care composition and hair management and/or treatment techniques and capable of repairing damaged hair by multiple mechanisms simultaneously and synergistically.

STATEMENT OF THE DISCLOSURE

Accordingly, the present disclosure describes a hair care composition that has excellent hair management and treatment property. The hair care composition affects and/or works on multiple targets simultaneously and synergistically, such as disulphide bond formation, amide bond formation and boosting the level of amino acids in the hair shaft, while managing and treating the hair, unlike the conventional hair care composition and treatment techniques.

The present disclosure describes a composition comprising monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids.

The present disclosure further describes a method of preparing the composition, said method comprises- mixing the monounsaturated carboxylic acid or its derivatives, the transglutaminase and the amino acid blend comprising at least 5 amino acids to obtain the composition.

The present disclosure further describes a formulation comprising effective amount of the composition described above.

The present disclosure further describes a method for managing and/or treating hair, said method comprises applying the composition or the formulation to hair for managing and/or treating the hair. BRIEF DESCRIPTION OF THE ACCOMPANYING FIGURES

In order that the present disclosure may be readily understood and put into practical effect, reference will now be made to exemplary embodiments as illustrated with reference to the accompanying figures. The figures together with detailed description below, are incorporated in and form part of the specification, and serve to further illustrate the embodiments and explain various principles and advantages, where:

Figure 1 provides Scanning electron microscopy (SEM) analysis images (500X magnification) of i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hair treated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure.

Figure 2 provides Scanning electron microscopy (SEM) analysis images (1000X magnification) of i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hair treated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure.

Figure 3 provides Fourier-transform infrared spectroscopy (FT-IR) analysis plot of- i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hair treated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure.

DETAILED DESCRIPTION OF THE DISCLOSURE

Unless otherwise defined, all terms used in the disclosure, including technical and scientific terms, have meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. By means of further guidance, term definitions are included for better understanding of the present disclosure.

As used herein, the singular forms 'a', 'an' and 'the' include both singular and plural referents unless the context clearly dictates otherwise.

The term 'comprising', 'comprises' or 'comprised of' as used herein are synonymous with 'including', 'includes', 'containing' or 'contains' and are inclusive or open-ended and do not exclude additional, non-recited members, elements or method steps.

The recitation of numerical ranges by endpoints includes all numbers and fractions subsumed within the respective ranges, as well as the recited endpoints.

The term 'about' as used herein when referring to a measurable value such as a parameter, an amount, a temporal duration, and the like, is meant to encompass variations of ±10% or less, preferably ±5% or less, more preferably ±1% or less and still more preferably ±0.1% or less of and from the specified value, insofar such variations are appropriate to perform the present disclosure. It is to be understood that the value to which the modifier 'about' refers is itself also specifically, and preferably disclosed.

Reference throughout this specification to 'some embodiments', 'one embodiment' or 'an embodiment' means that a particular feature, structure or characteristic described in connection with the embodiment may be included in at least one embodiment of the present disclosure, thus, the appearances of the phrases 'in some embodiments', 'in one embodiment' or 'in an embodiment' in various places throughout this specification may not necessarily all refer to the same embodiment. It is appreciated that certain features of the disclosure, which are for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the disclosure, which are, for brevity described in the context of a single embodiment, may also be provided separately or in any suitable sub-combination.

As used herein, 'treatment', 'treating of hair' or 'hair treatment' refers to cosmetic treatment, which does not involve any kind of therapy. In other words, the treatment or treating described herein refers to application of the composition of the present disclosure for purely cosmetic purposes and that there is no involvement of any kind of therapy.

As used herein, 'managing/management', 'hair managing/management',' or 'managing/management of hair' refers to application of the composition of present disclosure for purely cosmetic and cosmeceutical purposes involving hair detangling, hair fizz control, hair conditioning, hair nourishment, prevention of hair drying, increase in hair volume, cuticle sealing, strengthening of hair, improving hair smoothness, decreasing hair splits and softening of hair. However, does not involve any kind of therapy.

The present disclosure describes a hair care composition.

The hair care composition of the present disclosure provides for excellent hair treatment and hair management without causing any negative and/or side effect.

The hair care composition of the present disclosure affects and/or works on multiple targets synergistically in the hair. The hair care composition simultaneously provides for disulphide bond formation, amide bond formation and boosting the level of amino acids in hair shaft, unlike known or commercially available hair care composition or treatment techniques which affect single targets.

In some embodiments of the present disclosure, the hair care composition comprises monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids. In some embodiments of the present disclosure, the hair care composition comprises monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising 5 amino acids, 6 amino acids, 7 amino acids, 8 amino acids, 9 amino acids, 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids or 18 amino acids.

In some embodiments of the present disclosure, the monounsaturated carboxylic acid is selected from a group comprising itaconic acid, maleic acid, patenedioic acid, hexedioic acid, hexenoic acid, octenedioic acid and any combinations thereof.

In some embodiments of the present disclosure, derivatives of the monounsaturated carboxylic acid are selected from a group comprising ester of monounsaturated carboxylic acid, isomer of monounsaturated carboxylic acid, amide of monounsaturated carboxylic acid, salt of monounsaturated carboxylic acid and any combinations thereof.

In an embodiment, the ester of the monounsaturated carboxylic acid is selected from a group comprising, Bis(2-ethylhexyl) maleate, dicaprylyl maleate, diethyl 2-butenedioate, diisostearyl fumarate and any combinations thereof.

In an embodiment, the amide of the monounsaturated carboxylic acid is selected from a group comprising maleamide, crotonamide, 3-butenamide and any combinations thereof.

In an embodiment, the salts of the monounsaturated carboxylic acid is selected from a group comprising Disodium maleate, disodium fumarate, sodium itaconate and any combinations thereof.

In an embodiment, the isomer of the monounsaturated carboxylic acid is selected from a group comprising Fumaric acid, mesaconic acid, citraconic acid and any combinations thereof.

In some embodiments of the present disclosure, the monounsaturated carboxylic acid is in an amount ranging from about 1% to 15%, including all the values in the range, for instance,

1.1%, 1.2%, 1.3%, 1.4% and so on and so forth. In some embodiments of the present disclosure, derivatives of the monounsaturated carboxylic acid are in an amount ranging from about 1% to 15%, including all the values in the range, for instance, 1.1%, 1.2%, 1.3%, 1.4% and so on and so forth.

In some embodiments of the present disclosure, the transglutaminase (TGs) is selected from a group comprising TGI, TG2, TG3, TG4, TG5, TG6, TG7, Plasma TG and any combinations thereof.

In some embodiments of the present disclosure, the transglutaminase is in an amount ranging from about 0.001% to 1%, including all the values in the range, for instance, 0.002%, 0.003%, 0.004%, 0.005% and so on and so forth.

In some embodiments of the present disclosure, the amino acid blend comprises amino acids selected from a group comprising. Cysteine, Serine, Glutamic Acid, Threonine, Glycine, Leucine, Valine, Arginine, Aspartic Acid, Alanine, Proline, Isoleucine, Tyrosine, Phenylalanine, Histidine, Methionine, Lysine, Tryptophan and any combinations thereof.

In some embodiments of the present disclosure, the amino acid blend is selected from a group comprising blend of Alanine, Arginine, Proline, Tyrosine and Cysteine; blend of Cysteine, Histidine, Phenylalanine, Methionine and Isoleucine; blend of Glutamic acid, Arginine, Valine, Leucine and Alanine; blend of Aspartic Acid, Alanine, Proline, Arginine and Methionine; blend of Serine, Threonine, Glycine, Lysine and Tryptophan; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine and Isoleucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine and Phenylalanine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine and Methionine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acid; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid and Leucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine and Valine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threonine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine and Glycine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine and Aspartic Acid; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid and Isoleucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophan.

In some embodiments of the present disclosure, the amino acid blend is in an amount ranging from about 0.05% to 10%, including all the values in the range, for instance, 0.06%, 0.07%, 0.08%, 0.09% and so on and so forth.

In some embodiments of the present disclosure, in the amino acid blend comprising 5 amino acids, 6 amino acids, 7 amino acids, 8 amino acids, 9 amino acids, 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids or 18 amino acids, the amino acids are present in same proportion.

In some embodiments of the present disclosure, in the amino acid blend comprising 5 amino acids, 6 amino acids, 7 amino acids, 8 amino acids, 9 amino acids, 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids or 18 amino acids, the amino acids are present in different proportions.

In some embodiments of the present disclosure, in the amino acid blend comprising 5 amino acids, 6 amino acids, 7 amino acids, 8 amino acids, 9 amino acids, 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids or 18 amino acids, the amino acids are present in different proportions as present in virgin hair.

In some embodiments of the present disclosure, the composition comprises solvent includes but it is not limited to water. In an embodiment, the solvent is present in sufficient amount for making the composition, i.e., quantum sufficit (q.s.). In some embodiments of the present disclosure, the composition comprises water in sufficient amount for making the composition, i.e., quantum sufficit (q.s.).

In an exemplary embodiment of the present disclosure, the composition comprises about 1% to about 15% of the monounsaturated carboxylic acid or its derivative; about 0.001% to 1% of the transglutaminase; about 0.05% to 10% of amino acid blend comprising at least 5 amino acids and solvent is present in sufficient quantity.

In another exemplary embodiment of the present disclosure, the composition comprises about 5% of the monounsaturated carboxylic acid or its derivative; about 1.0% of the amino acid blend comprising at least 5 amino acids; about 0.6% of the transglutaminase; and solvent is present in sufficient quantity.

In some embodiments of the present disclosure, the composition comprises chelating agent. In an embodiment, the chelating agent is selected from a group comprising Ethylenediaminetetraacetic acid, Disodium ethylenediaminetetraacetic acid, Trisodium Ethylenediamine Disuccinate, Dipotassium ethylenediaminetetraacetic acid, Diethyl oxalate and any combinations thereof.

In some embodiments of the present disclosure, the chelating agent is present in an amount ranging from about 0.05% to 1%, including all the values in the range, for instance, 0.06%. 0.07%, 0.08%, 0.09% and so on and so forth.

In an exemplary embodiment of the present disclosure, the chelating agent is present in an amount ranging from about 0.05% to 0.1%. In an embodiment, the chelating agent is present in an amount of about 0.05%, about 0.06%, about 0.07%, about 0.08%, about 0.09% or about 0.1%.

In some embodiments of the present disclosure, the composition has pH ranging from about 2 to 11, including all the values in the range, for instance, 2.1, 2.2, 2.3, 2.4 and so on and so forth. The inventors of the present application have particularly identified that the combination of monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids provides for improved hair treatment and hair management, which is not observed while using components individually or a composition comprising combination of two components, respectively. The inventors have identified that the composition comprising combination of monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids, affects and/or works on multiple targets simultaneously and synergistically, such as disulphide bond formation, amide bond formation and boosting the level of amino acid in hair shaft, which is not the case when the components are employed individually or a composition comprising combination of two components.

The figures 1 and 2 describes improved effect (as per SEM analysis) of the composition of the present disclosure in hair management and treatment, wherein surprising effect of the composition can be noted vis-a-vis individual components and composition comprising combination of two components, respectively. Similarly, Figure 3 describes improved effect (as per FT-IR analysis) of the composition of the present disclosure in hair management and treatment over individual components and composition comprising combination of two components, respectively.

The present disclosure further describes a method of preparing the composition described above.

In some embodiments of the present disclosure, the method of preparing the composition comprises- mixing the monounsaturated carboxylic acid or its derivative, the transglutaminase and the amino acid blend comprising at least 5 amino acids.

In some embodiments of the present disclosure, the method of preparing the composition comprises- mixing the monounsaturated carboxylic acid or its derivative, the transglutaminase and the amino acid blend comprising 5 amino acids, 6 amino acids, 7 amino acids, 8 amino acids, 9 amino acids, 10 amino acids, 11 amino acids, 12 amino acids, 13 amino acids, 14 amino acids, 15 amino acids, 16 amino acids, 17 amino acids or 18 amino acids. In some embodiments of the present disclosure, the method of preparing the composition comprises: a) adding the monounsaturated carboxylic or its derivative to a mixture comprising the solvent and the chelating agent, followed by stirring; b) adding the transglutaminase, followed by stirring; and c) adding the amino acid blend comprising at least 5 amino acids and stirring to obtain the composition.

In an embodiment, the stirring in the step a) is carried out at a temperature ranging from about 20 °C to 60 °C, including all the values in the range, for instance, 21 °C, 22 °C, 23 °C, 24 °C and so on and so forth, for a duration ranging from about 1 minute to 90 minutes, including all the values in the range, for instance, 2 minutes, 3 minutes, 4 minutes, 5 minutes and so on and so forth. In an embodiment, the stirring is carried out until clear solution is obtained.

In an embodiment, the stirring in the step b) is carried out at a temperature ranging from about 20 °C to 60 °C, including all the values in the range, for instance, 21 °C, 22 °C, 23 °C, 24 °C and so on and so forth, for a duration ranging from about 1 minute to 60 minutes, including all the values in the range, for instance, 2 minutes, 3 minutes, 4 minutes, 5 minutes and so on and so forth. In an embodiment, the stirring is carried out until clear solution is obtained.

In an embodiment, the stirring in the step c) is carried out at a temperature ranging from about 20 °C to 60 °C, including all the values in the range, for instance, 21 °C, 22 °C, 23 °C, 24 °C and so on and so forth, for a duration ranging from about 1 minute to 90 minutes, including all the values in the range, for instance, 2 minutes, 3 minutes, 4 minutes, 5 minutes and so on and so forth.

In some embodiments, in the method of preparing the composition, upon adding the monounsaturated carboxylic acid or its derivative, the pH of the solution is maintained in a range of about 2 to 11, including all the values in the range, for instance, 2.1, 2.2, 2.3, 2.4 and so on and so forth.

The present disclosure further relates to a formulation comprising the composition described above in an effective amount. In some embodiment, the formulation comprises the composition in an effective amount and excipient.

In some embodiments, in the formulation, the composition is present in an amount ranging from about 1% to 25%, including all the values in the range, for instance, 1.1%, 1.2%, 1.3%, 1.4% and so on and so forth.

In some embodiments, in the formulation, the excipient is present in an amount ranging from about 1% to 99%, including all the values in the range, for instance, 0.2%, 0.3%, 0.4%, 0.5% and so on and so forth.

In some embodiments, the excipient is selected from a group comprising Corrosion Inhibitors, Cosmetic Astringents, Denaturants, Deodorant Agents, Surfactant, Opacifiers, Chelating Agent, Emulsifier, Preservative, Thickeners, Emollient, Cosolvent, Abrasives, Anticaking Agent, Anticaries Agents, Antifoaming Agents, Antiperspirants, Antistatic Agents, Artificial Nail Builders, Depilating Agents, Bulking Agents, Colorants, Perfuming, Dispersing Agents, occlusive, Astringents, Emulsion Stabilizers, Epilating Agents, Exfoliants, Eyelash Conditioning Agents, Film Formers, Flavoring Agents, Fragrance Ingredients, Hair Colorants, Hair Conditioning Agents, Hair Fixatives, Hair Waving/Straightening Agents, Humectants, Light Stabilizers, Lytic Agents, Nail Conditioning Agents, Opacifying Agents, Oxidizing Agents, gelling agents and any combinations thereof.

In some embodiments, the formulation comprises solvent including but it is not limited to water. In an embodiment, the solvent is present in sufficient amount for making the composition, i.e., quantum sufficit (q.s.).

In some embodiments, the formulation has pH ranging from about 2 to 11, including all the values in the range, for instance, 2.1, 2.2, 2.3, 2.4 and so on and so forth.

In some embodiments, the formulation is selected from a group comprising Hair Mask, Hair Cream, Hair Spray, Hair Serum, Hair Colour, Hair Lotion, Hair Wax, Hair Gel, Hair Balm, Hair Mousse, Hair Tonic, Hair Moisturizer, Hair Cleanser, Hair Butter, Hair Colour Remover, Hair Solution, Hair Pomade, Hair Volumizer, Hair Clay, Hair Cream, Hair Oil, Hair Stylers, Hair Heat Protector, Hair Conditioning Mask, Hair Root Pump, Dry Shampoo, Hair Texturizer, Hair Curl Retentioner, Heena Powder, Hair Growth Serum, Hair Drops, Hair Scalp Maintenance, Hair Scalp Exfoliator, Hair Scalp Scrub, Shampoo Bar, Conditioner Bar, Hair Concentrates and Hair Infusion.

The formulation of the present disclosure provides for improved hair management and hair treatment. The formulation affects and/or works on multiple targets simultaneously and synergistically, such as disulphide bond formation, amide bond formation and boosting the level of amino acid in hair shaft. The formulation provides excellent hair management and treatment.

The present disclosure further relates to method for managing and/or treating hair.

In some embodiments of the present disclosure, the method for managing and/or treating hair comprises- applying the composition described above or the formulation described above to hair for managing and/or treating the hair.

In some embodiments of the present disclosure, in the method, the managing and/or treating of hair comprises at least one of hair damage repair, free cysteine rearrangement, amide bond repair, disulphide bond repair, keratin repair and any combinations thereof.

In some embodiments of the present disclosure, in the method, the managing and/or treating of hair comprises hair damage repair, free cysteine rearrangement, amide bond repair, disulphide bond repair and keratin repair.

In some embodiments of the present disclosure, in the method of managing and/or treating hair, the composition is employed at a concentration ranging from about 1% to 25%, including all the values the range, for instance, 1.1%, 1.2%, 1.3%, 1.4% and so on and so forth.

In some embodiments of the present disclosure, the managed and/or treated hair shows up to 95% reduction in free cysteine when compared to bleached hair. In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in free cysteine in the range of about 13% to 95%, including all the values in the range, for instance, 13%, 14%, 15%, 16% and so on and so forth, when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in free cysteine in the range of about 13% to 95%, including all the values in the range, for instance, 13%, 14%, 15%, 16% and so on and so forth, when compared to hair not applied with the composition or the formulation of the present disclosure.

In some embodiments of the present disclosure, the managed and/or treated hair shows up to about 81% reduction in combining force in wet hair when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in combining force in wet hair in the range of about 28% to 81%, including all the values in the range, for instance, 29%, 30%, 31%, 32% and so on and so forth, when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in combining force in wet hair in the range of about 28% to 81%, including all the values in the range, for instance, 29%, 30%, 31%, 32% and so on and so forth, when compared to hair not applied with the composition or the formulation of the present disclosure.

In some embodiments of the present disclosure, the managed and/or treated hair shows about 87% reduction in combining force in dry hair when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in combining force in dry hair in the range of about 46% to 87%, including all the values in the range, for instance, 47%, 48%, 49%, 50% and so on and so forth, when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows reduction in combining force in dry hair in the range of about 46% to 87%, including all the values in the range, for instance, 47%, 48%, 49%, 50% and so on and so forth, when compared to hair not applied with the composition or the formulation of the present disclosure.

In some embodiments of the present disclosure, the managed and/or treated hair has increased tensile stress up to 48% when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair has tensile stress in the range of about 6% to 48%, including all the values in the range, for instance, 7%, 8%, 9%, 10% and so on and so forth. In an embodiment, the managed and/or treated hair applied with the composition or the formulation of the present disclosure has higher tensile stress when compared to bleached hair.

In some embodiments of the present disclosure, the managed and/or treated hair shows increased tensile stress in the range of about 6% to 48%, including all the values in the range, for instance, 7%, 8%, 9%, 10% and so on and so forth. In an embodiment, the managed and/or treated hair applied with the composition or the formulation of the present disclosure has higher tensile stress when compared to hair not applied with the composition or the formulation of the present disclosure.

In an embodiment, the Figure 1 provides Scanning electron microscopy (SEM) analysis images (500X magnification) of i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hairtreated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure. From the images, it can be noted that the bleaching of hair caused significant damage in the hair cuticles. It can be noted that when bleached hair was treated with individual components of the composition, hair showed negligible recovery from the damages caused by bleaching. Further, it can be noted that when the bleached hair was treated with composition comprising combination of two components there was only marginal recovery from the damages caused by bleaching. However, it was surprisingly noted that the bleached hair treated with the composition of the present disclosure showed significant recovery from the damages caused by bleaching [see figure 1 (viii)]. It can be noted that cuticles of the hair were found be repaired with smooth texture. Thus, clearly establishing that the composition of the present disclosure has synergistic effect in treating and/or managing hair.

In an embodiment, the Figure 2 provides Scanning electron microscopy (SEM) analysis images (1000X magnification) of i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hairtreated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure. From the images, it can be noted that the bleaching of hair caused significant damage in the hair cuticles. It can be noted that when bleached hair was treated with individual components of the composition, hair showed negligible recovery from the damages caused by bleaching. Further, it can be noted that when the bleached hair was treated with composition comprising combination of two components there was only marginal recovery from the damages caused by bleaching. However, it was surprisingly noted that the bleached hair treated with the composition of the present disclosure showed significant recovery from the damages caused by bleaching [see figure 2 (viii)]. It can be noted that cuticles of the hair were found be repaired with smooth texture. Thus, clearly establishing that the composition of the present disclosure has synergistic effect in treating and/or managing hair.

In an embodiment, the Figure-3 provides Fourier-transform infrared spectroscopy (FT-IR) analysis plot of- i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hairtreated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure. From the FT-IR spectrum, it can be noted that, the bleached hair showed a peak at 1042 cm ¹ which attributes to the presence of cysteic acid. Furthermore, same peak was observed between 1045-1040 cm ¹ in the FT-IR spectrum of hair treated with individual components of the composition and combination of two components. However, this peak is absent in the spectra of hair treated with the composition of the present disclosure suggested absence of cysteic acid.

The composition, the formulation and the method of managing and treating hair of the present disclosure provides for the following advantages.

The composition and/or the formulation affects and/or works on multiple targets simultaneously and synergistically, such as disulphide bond formation, amide bond formation and boosting the level of amino acid in hair shaft.

The composition and/or the formulation of the present disclosure provides for hair damage repair, free cysteine rearrangement, amide bond repair, disulphide bond repair, keratin repair, hair detangling, frizz control and hair softness.

It is to be understood that the foregoing description is illustrative not a limitation. While considerable emphasis has been placed herein on particular features of this disclosure, it will be appreciated that various modifications can be made, and that many changes can be made in the preferred embodiments without departing from the principles of the disclosure. Those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein. Similarly, additional embodiments and features of the present disclosure will be apparent to one of ordinary skill in art based upon description provided herein.

Descriptions of well-known/conventional methods/steps and techniques are omitted so as to not unnecessarily obscure the embodiments herein. Further, the disclosure herein provides for examples illustrating the above-described embodiments, and in order to illustrate the embodiments of the present disclosure, certain aspects have been employed. The examples used herein for such illustration are intended merely to facilitate an understanding of ways in which the embodiments may be practiced and to further enable those of skill in the art to practice the embodiments. Accordingly, following examples should not be construed as limiting the scope of the embodiments herein. EXAMPLES

Example 1: Preparation of the Composition

About 98.6 gm water and about 0.1 chelating agent were mixed to obtain a solution. To the solution, about 1% of Maleic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4-5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine and Cysteine was added and stirred to obtain the composition.

Example 2: Preparation of the Composition

About 96.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution about 3% of Itaconic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4-5. About O.lgm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2g of amino acid blend comprising Cysteine, Histidine, Phenylalanine, Methionine and Isoleucine was added and stirred to obtain the composition.

Example 3: Preparation of the Composition

About 96.6 gm water and about 0.1 gm chelating agent are mixed to obtain a solution. To the solution about 3% of fumaric acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4-5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 of amino acid blend comprising Glutamic acid, Arginine, Valine, Leucine and Alanine was added and stirred to obtain the composition.

Example 4: Preparation of the Composition

About 89.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution about 10 gm of hexenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4-5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Aspartic Acid, Alanine, Proline, Arginine and Methionine was added and stirred to obtain the composition. Example 5: Preparation of the Composition

About 84.6 gm water and about O.lgm chelating agent were mixed to obtain a solution. To the solution about 15 gm of Octenedioc acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4-5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2gm of amino acid blend comprising Serine, Threonine, Glycine, Lysine and Tryptophan was added and stirred to obtain the composition.

Example 6: Preparation of the Composition

About 96.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 3 gm of Itaconic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine was added and stirred to obtain the composition.

Example 7: Preparation of the Composition

About 98.3 gm water and about 0.1 g chelating agent were mixed to obtain a solution. To the solution, about 1 gm of Maleic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.5 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine; was added and stirred to obtain the composition.

Example 8: Preparation of the Composition

About 96.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 3 gm of Fumaric acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine was added and stirred to obtain the composition. Example 9: Preparation of the Composition

About 89.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 10 gm of Hexenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine was added and stirred to obtain the composition.

Example 10: Preparation of the Composition

About 84.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 15 gm of Octenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine was added and stirred to obtain the composition.

Example 11: Preparation of the Composition

About 96.3 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 3 gm of Itaconic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.5 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acidwas added and stirred to obtain the composition.

Example 12: Preparation of the Composition

About 94.2 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 5 gm of Maleic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.2 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.5 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acidwas added and stirred to obtain the composition.

Example 13: Preparation of the Composition

About 93.8 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 5 gm of Fumaric acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 1 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acid was added and stirred to obtain the composition.

Example 14: Preparation of the Composition

About 88.7 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 10 gm of Hexenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.2 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 1 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acid was added and stirred to obtain the composition.

Example 15: Preparation of the Composition

About 82.7 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 15 gm of Octenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.2 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acidwas added and stirred to obtain the composition.

Example 16: Preparation of the Composition About 96.7 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 1 gm of Itaconic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.2 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threonine was added and stirred to obtain the composition.

Example 17: Preparation of the Composition

About 93.7 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 3 gm of Maleic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.2 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 3 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threonine was added and stirred to obtain the composition.

Example 18: Preparation of the Composition

About 91.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 5 gm of Fumaric acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.3 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 3 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threoninewas added and stirred to obtain the composition.

Example 19: Preparation of the Composition

About 85.5 gm water and about 0.1 gm chelating agent are mixed to obtain a solution. To the solution, about 10 gm of Hexenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.4 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 4 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threoninewas added and stirred to obtain the composition.

Example 20: Preparation of the Composition

About 79.4 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 15 gm of Octenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.5 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 5 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threoninewas added and stirred to obtain the composition.

Example 21: Preparation of the Composition

About 93.4 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 1 gm of Itaconic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.5 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 5 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophanwas added and stirred to obtain the composition.

Example 22: Preparation of the Composition

About 96.6 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 3 gm of Maleic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.2 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophanwas added and stirred to obtain the composition.

Example 23: Preparation of the Composition About 93.3 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 5 gm of Fumaric acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.8 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.8 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophan was added and stirred to obtain the composition.

Example 24: Preparation of the Composition

About 88.3 gm water and about 0.1 gm chelating agent were mixed to obtain a solution. To the solution, about 10 gm of Hexenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 0.8 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 0.8 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophan was added and stirred to obtain the composition.

Example 25: Preparation of the Composition

About 73.9 gm water and about 0.1 gm chelating agent are mixed to obtain a solution. To the solution, about 15 gm of Octenedioic acid was added, followed by stirring until clear solution was obtained. Further, pH of the solution was adjusted to about 4 - 5. About 1 gm transglutaminase was added, followed by stirring until clear solution was obtained. About 10 gm of amino acid blend comprising Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophan was added and stirred to obtain the composition.

Example 26: Preparation of the Formulation

Formulations obtained according to the compositions of the present disclosure are provided below

Formulation for Hair Mask:

Formulation-1:

Formulation-2:

Formulation-3:

Formulation for Hair Shampoo:

Formulation 1:

Formulation 2:

Formulation 3:

Formulation for Hair Serum:

Formulation 1:

Formulation 2:

Formulation 3:

Example 27: Comparative Example

In this example, the hair is treated with- i. the composition of the present disclosure; ii. individual components (maleic acid, amino acid blend comprising at least 5 amino acids, transglutaminase, respectively); and iii. composition comprising combination of two components, independently- a) maleic acid and amino acid blend comprising at least two amino acids b) transglutaminase and amino acid blend comprising at least two amino acids; and c) maleic acid and transglutaminase bleached was used control

Free Cysteine content in the hair:

Table 1:

The data in Table 1 demonstrates that the hair treated with the composition of the present disclosure has significantly reduced cysteine content when compared to hair treated with individual components and hair treated with composition comprising combination of two components (SI. No. 2, 3 and 4). From the data in the Table 1 it can be inferred that the composition of the present disclosure has higher efficacy to keep the disulfide bond aligned and intact in the hair fibres, thus leading to significantly reduced free cysteine content, when compared to composition comprising combination of two components. According to scanning electron microscopy, no effect was noted when individual components for the composition were applied separately.

It must be noted that higherthe amount of free cysteine in hair represents higher the damage level of the hair fibre.

Combining Force:

Combining force represents cuticle alignment of hair fibres. Lower the combining force means more aligned unidirectional cuticle, i.e., improved managing of hair.

Table 2:

Data in Table 2 demonstrates that the hair treated with the composition of the present disclosure has significantly reduced combining force, implying that the hair has higher aligned unidirectional cuticle when compared to hair treated with individual components and composition comprising combination of two components (Sr. No. 2, 3 and 4), respectively. According to scanning electron microscopy, no effect was noted when individual components were applied separately.

Tensile Stress:

Tensile stress or break stress represents overall strength of the hair fibres. Higher the tensile stress/ break stress means higher the tensile strength and less fragility of hair fibres.

Table 3:

Data in Table 3 demonstrates that the hair treated with the composition of the present disclosure has significantly higher tensile stress which attributes to increased tensile strength and lower fragility of hair when compared to hair treated with composition comprising combination of two components (Sr. No. 2, 3 and 4), respectively.

FT-IR Analysis:

Figure 3 shows FT-IR spectrums of- i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hair treated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure.

Table 4 provides peaks noted in the FT-IR spectrum along with their description

Table 4: i. The FT-IR spectrum of bleached hair and hair treated with individual components and composition comprising combination of two components, showed a peak between 1045-1040 cm ¹ which represent the presence of cysteic acid. While the hair treated with the composition of the present disclosure did not show peak between 1045-1041 cm⁴. Thus, it was noted that the composition of the present disclosure showed improved efficacy in converting free cysteine into the cystine (disulphide bonding), as a result free cysteine would not be available to oxidize into cysteic acid in the hair treated with the composition of the present disclosure. ii. The FT-IR spectrum of bleached hair and hair treated with individual components and composition comprising combination of two components, showed a peak between 1115-1089 cm ¹ which represent conversion of free cysteine to its sulphur monoxide derivative. While the hair treated with the composition of the present disclosure did not show peak between 1115-1089 cm ¹ along with the cysteic acid peak (~1040 cm ) as mentioned above. This implying that there was no conversion of free cysteine to cysteic acid in the hair treated with the composition of present disclosure. iii. The FT-IR spectrum of bleached hair and hair treated with the individual components and composition comprising combination of two components showed a sharp peak between 1739-1738 cm ¹ which represents the deformation of amide bonds. The intensity of this peak decreased drastically in the FT-IR spectra of hair treated with the composition of the present disclosure, implying that there was insignificant or no amide bond deformation. iv. The FT-IR spectrum of bleached hair and hair treated with individual components and a composition comprising combination of two components showed peaks between 1388-1365 & 1220-1215 cm ¹ which represent the presence of SO2 functionality that is due to the conversion of free cysteine to cysteic acid in the hair. The intensity of these peaks decreased significantly in the FT-IR spectra of hair treated with the composition of the present disclosure, implying that there was remarkably less conversion of free cysteine to cysteic acid.

Figures 1 and 2 provides SEM analysis images (500X and 1000X magnification, respectively) of i. bleached hair; ii. hair treated with amino acid; iii. hair treated with monounsaturated carboxylic acid (e.g., maleic acid); iv. Hair treated with transglutaminase; v. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and amino acid blend comprising at least 5 amino acids; vi. hair treated with combination of amino acid blend comprising at least 5 amino acids and transglutaminase; vii. hair treated with combination of monounsaturated carboxylic acid (e.g., maleic acid) and transglutaminase; and viii. hair treated with the composition of the present disclosure.

From the SEM images it can be noted that-

Bleached hair showed damaged cuticles. Hair treated with individual components and composition comprising combination of two components continued to show damaged cuticles, respectively.

Hair treated with the composition of the present disclosure showed complete cuticle repair.

Additional embodiments and features of the present disclosure will be apparent to one of ordinary skill in art based on the description provided herein. The embodiments herein provide various features and advantageous details thereof in the description. Descriptions of well-known/conventional methods and techniques are omitted so as to not unnecessarily obscure the embodiments herein.

The foregoing description of the specific embodiments fully reveals the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and/or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is forthe purpose of description and not of limitation. Therefore, while the embodiments in this disclosure have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein.

As regards the embodiments characterized in this specification, it is intended that each embodiment be read independently as well as in combination with another embodiment. For example, in case of an embodiment 1 reciting 3 alternatives A, B and C, an embodiment 2 reciting 3 alternatives D, E and F and an embodiment 3 reciting 3 alternatives G, H and I, it is to be understood that the specification unambiguously discloses embodiments corresponding to combinations A, D, G; A, D, H; A, D, I; A, E, G; A, E, H; A, E, I; A, F, G; A, F, H; A, F, I; B, D, G; B, D, H; B, D, I; B, E, G; B, E, H; B, E, I; B, F, G; B, F, H; B, F, I; C, D, G; C, D, H; C, D, I; C, E, G; C, E, H; C, E, I; C, F, G; C, F, H; C, F, I, unless specifically mentioned otherwise.

While considerable emphasis has been placed herein on the particular features of this disclosure, it will be appreciated that various modifications can be made, and that many changes can be made in the preferred embodiments without departing from the principles of the disclosure. These and other modifications in the nature of the disclosure or the preferred embodiments will be apparent to those skilled in the art from the disclosure herein, whereby it is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation.

Claims

WE CLAIM:

1. A composition comprising- monounsaturated carboxylic acid or its derivative, transglutaminase and amino acid blend comprising at least 5 amino acids.

2. The composition as claimed in claim 1, wherein the monounsaturated carboxylic acid or its derivative is selected from a group comprising itaconic acid, butenedioc acid, pentenedioc acid, hexenedioc acid, maleic acid, hexenoic acid, octeneoic acid, Bis(2- ethylhexyl) maleate, maleamide and any combinations thereof.

3. The composition as claimed in claim 1, wherein the transglutaminase (TGs) is selected from a group comprising TGI, TG2, TG3, TG4, TG5, TG6, TG7, Plasma TG and any combination thereof.

4. The composition as claimed in claim 1, wherein the amino acid blend comprises amino acids selected from a group comprising Cysteine, Serine, Glutamic Acid, Threonine, Glycine, Leucine, Valine, Arginine, Aspartic Acid, Alanine, Proline, Isoleucine, Tyrosine, Phenylalanine, Histidine, Methionine, Lysine, Tryptophan and any combinations thereof.

5. The composition as claimed in claim 1, wherein the amino acid blend is selected from a group comprising blend of Alanine, Arginine, Proline, Tyrosine and Cysteine; blend of Cysteine, Histidine, Phenylalanine, Methionine and Isoleucine; blend of Glutamic acid, Arginine, Valine, Leucine and Alanine; blend of Aspartic Acid, Alanine, Proline, Arginine and Methionine; blend of Serine, Threonine, Glycine, Lysine, Tryptophan blend of Alanine, Arginine, Proline, Tyrosine, Cysteine and Isoleucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine and Phenylalanine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine and Histidine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine and Methionine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine and Glutamic acid; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid and Leucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine and Valine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine and Threonine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine and Glycine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine and Aspartic Acid; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid and Isoleucine; blend of Alanine, Arginine, Proline, Tyrosine, Cysteine, Isoleucine, Phenylalanine, Histidine, Methionine, Glutamic acid, Leucine, Valine, Threonine, Glycine, Aspartic Acid, Isoleucine and Tryptophan. The composition as claimed in claim 1, wherein the monounsaturated carboxylic acid is in an amount ranging from about 1% to 15%. The composition as claimed in claim 1, wherein the transglutaminase is in an amount ranging from about 0.001% to 1%. The composition as claimed in claim 1, wherein the amino acid blend is in an amount ranging from about 0.05% to 10%. The composition as claimed in claim 1, wherein the composition further comprises chelating agent selected from a group comprising Ethylenediaminetetraacetic acid, Disodium ethylenediaminetetraacetic, Trisodium Ethylenediamine Disuccinate, Dipotassium ethylenediaminetetraacetic acid, Diethyl oxalate and any combinations thereof; and wherein the chelating agent is in an amount ranging from about 0.05% to 1%. The composition as claimed in claim 1, wherein the composition comprises water q.s. (quantum sufficit). A method of preparing the composition as claimed in claim 1, said method comprises- mixing the monounsaturated carboxylic acid or its derivative, the transglutaminase and the amino acid blend comprising at least 5 amino acids to obtain the composition. The method as claimed in claim 11, wherein the method comprises adding the monounsaturated carboxylic acid to mixture of chelating agent and water, followed by adding the transglutaminase and the amino acid blend comprising at least 5 amino acids. A formulation comprising the composition as claimed in claim 1. The formulation as claimed in claim 13, wherein the composition is in an amount ranging from about 1% to 25%. The formulation as claimed in claim 13, wherein the formulation comprises excipient in an amount ranging from about 1% to 99%; and wherein the excipient is selected from a group comprising Corrosion Inhibitors, Cosmetic Astringents, Cosmetic Biocides, Denaturants, Deodorant Agents, Surfactant, opacifiers chelating agent, emulsifier, preservative, thickeners, emollient, solvents, cosolvent, abrasives, anticaking agent, anticaries agents, antifoaming agents, antiperspirants, antistatic agents, artificial nail builders, Depilating Agents, bulking agents, colorants, perfuming, Dispersing Agents, occlusive, Astringents, Emulsion Stabilizers, Epilating Agents, Exfoliants, Eyelash Conditioning Agents, Film Formers, Flavouring Agents, Fragrance Ingredients, Hair Colorants, Hair Conditioning Agents, Hair Fixatives, Hair- Waving/Straightening Agents, Humectants, Light Stabilizers, Lytic Agents, Nail Conditioning Agents, Opacifying Agents, Oxidizing Agents, gelling agents and any combinations thereof. The formulation as claimed in claim 13, wherein the formulation is selected from a group comprising Hair mask, Hair Cream, Hair Spray, Hair Serum, Hair Colour, Hair Lotion, Hair Wax, Hair Gel, Hair Balm, Hair Mousse, Hair Tonic, Hair Moisturizer, Hair Cleanser, Hair Butter, Hair Colour Remover, Hair Solution, Hair Pomade, Hair Volumizer, Hair Clay, Hair Cream Oil, Hair Stylers, Hair Heat Protector, Hair Conditioning Mask, Hair Root Pump, Dry Shampoo, Hair Texturizer, Hair Curl Retentioner, Heena Powder, Hair Growth Serum, Hair Drops, Hair Scalp Maintenance, Hair Scalp Exfoliator, Hair Scalp Scrub, Shampoo Bar, Conditioner Bar, Hair Concentrates and Hair infusion. A method for managing and/or treating hair, said method comprises applying the composition as claimed in claim 1 or the formulation as claimed in claim 14 to hair for managing and/or treating the hair. The method as claimed in claim 17, wherein the managing hair comprises Hair damage repairing, free Cysteine rearrangement, amide bond repair, disulfide bond repair, keratin repair and any combination thereof. The method as claimed in claim 17, wherein the composition is at a concentration ranging from about 1% to 25. The method as claimed in claim 17, wherein the managed hair shows reduction in free cysteine content in the range of about 13% to 95%. The method as claimed in claim 17, wherein the managed hair shows reduction in combining force in the range of about 28% to 81% for wet hair and shows reduction in combining force in the range of about 46% to 87% for dry hair. The method as claimed in claim 17, wherein the managed hair has tensile stress ranging from about 6% to 48%.