WO2024069493A3 - Methods for separating multiplexed beads - Google Patents

Methods for separating multiplexed beads Download PDF

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Publication number
WO2024069493A3
WO2024069493A3 PCT/IB2023/059643 IB2023059643W WO2024069493A3 WO 2024069493 A3 WO2024069493 A3 WO 2024069493A3 IB 2023059643 W IB2023059643 W IB 2023059643W WO 2024069493 A3 WO2024069493 A3 WO 2024069493A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
sample
methods
bead
separating multiplexed
Prior art date
Application number
PCT/IB2023/059643
Other languages
French (fr)
Other versions
WO2024069493A2 (en
Inventor
Chang Liu
Thomas R. Covey
Original Assignee
Dh Technologies Development Pte. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dh Technologies Development Pte. Ltd. filed Critical Dh Technologies Development Pte. Ltd.
Publication of WO2024069493A2 publication Critical patent/WO2024069493A2/en
Publication of WO2024069493A3 publication Critical patent/WO2024069493A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36 using an external stimulus
    • B01D15/3885—Using electrical or magnetic means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00564—Handling or washing solid phase elements, e.g. beads

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Of Solids By Using Liquids Or Pneumatic Power (AREA)

Abstract

A method of processing a liquid sample includes providing the sample. A first set of beads is introduced to the sample. The first set of beads includes a bead characteristic and a first bead performance value. A second set of beads is introduced to the sample. The second set of beads includes the bead characteristic and a second bead performance value different than the first bead performance value. The sample is mixed with the first set of beads and the second set of beads. The first set of beads is captured in a first location within the sample.
PCT/IB2023/059643 2022-09-29 2023-09-27 Methods for separating multiplexed beads WO2024069493A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263411398P 2022-09-29 2022-09-29
US63/411,398 2022-09-29

Publications (2)

Publication Number Publication Date
WO2024069493A2 WO2024069493A2 (en) 2024-04-04
WO2024069493A3 true WO2024069493A3 (en) 2024-06-06

Family

ID=88297324

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2023/059643 WO2024069493A2 (en) 2022-09-29 2023-09-27 Methods for separating multiplexed beads

Country Status (1)

Country Link
WO (1) WO2024069493A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002090565A2 (en) * 2001-05-07 2002-11-14 Gamida Volcano Ltd. Magnetic beads and uses thereof
US20130337455A1 (en) * 2012-05-03 2013-12-19 The Regents Of The University Of Michigan Multi-Mode Separation for Target Detection and Cell Growth Monitoring
WO2023121990A1 (en) * 2021-12-20 2023-06-29 Hach Company Methods and kits for assaying a large fluid volume using flow cytometry

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002090565A2 (en) * 2001-05-07 2002-11-14 Gamida Volcano Ltd. Magnetic beads and uses thereof
US20130337455A1 (en) * 2012-05-03 2013-12-19 The Regents Of The University Of Michigan Multi-Mode Separation for Target Detection and Cell Growth Monitoring
WO2023121990A1 (en) * 2021-12-20 2023-06-29 Hach Company Methods and kits for assaying a large fluid volume using flow cytometry

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JEGATHA NAMBI KRISHNAN ET AL: "Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis", ELECTROPHORESIS, VERLAG CHEMIE, HOBOKEN, USA, vol. 30, no. 9, 7 May 2009 (2009-05-07), pages 1457 - 1463, XP071499913, ISSN: 0173-0835, DOI: 10.1002/ELPS.200800646 *

Also Published As

Publication number Publication date
WO2024069493A2 (en) 2024-04-04

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