WO2024041209A1

WO2024041209A1 - Microfluidic chip

Info

Publication number: WO2024041209A1
Application number: PCT/CN2023/104889
Authority: WO
Inventors: 张赛; 祝亮; 何凡; 钱纯亘
Original assignee: 深圳市卓润生物科技有限公司
Priority date: 2022-08-22
Filing date: 2023-06-30
Publication date: 2024-02-29
Also published as: CN115518698A

Abstract

A microfluidic chip (100), comprising a chip body (1), at least one microfluidic valve (2), and liquid inlet cavities (30) and reaction cavities (40) respectively having one-to-one correspondence to the microfluidic valves (2). The chip body (1) has a rotation center axis; each microfluidic valve (2) comprises a Tesla valve pipeline (20); a liquid inlet (21) and a liquid outlet (22) are respectively formed at two opposite ends of the Tesla valve pipeline (20); the distance between the liquid inlet (21) and the rotation center axis is smaller than the distance between the liquid outlet (22) and the rotation center axis; the Tesla valve pipeline (20) is configured to form a preset resistance for a fluid flowing from the liquid inlet (21) to the liquid outlet (22); each liquid inlet cavity (30) is communicated with the liquid inlet (21) of the Tesla valve pipeline (20) of the corresponding microfluidic valve (2); and each reaction cavity (40) is communicated with the liquid outlet (22) of the Tesla valve pipeline (20) of the corresponding microfluidic valve (2).

Description

微流控芯片Microfluidic Chip

交叉引用cross reference

本申请引用于2022年08月22日递交的名称为“微流控芯片”的第202211006592.9号中国专利申请，其通过引用被全部并入本申请。This application cites Chinese patent application No. 202211006592.9 titled "Microfluidic Chip" submitted on August 22, 2022, which is fully incorporated into this application by reference.

技术领域Technical field

本申请涉及微流控技术领域，特别是涉及一种微流控芯片。The present application relates to the field of microfluidic technology, and in particular to a microfluidic chip.

背景技术Background technique

微流控芯片(microfluidic chip)是当前微全分析***(miniaturized total analysis systems)发展的热点领域，指将多种生物、化学、医学等领域的中所涉及的一系列实验，包括样品前处理、样品反应和结果读取等操作整合到一张具有微纳尺寸结构的芯片上。其中，离心微流控芯片，指的是利用离心力为动力驱动样品或试剂在芯片微管道中运动，从而进行检测的一类芯片。为了控制离心微流控芯片上液路的通断，相关技术中提供了一种微流控阀门，通过在微流控芯片上通过特殊的结构设计，如毛细管、表面改性、薄膜等结构起到抑制流体流动的作用，从而通过控制微流控阀门开闭实现液路的通断。Microfluidic chip is currently a hot area in the development of miniaturized total analysis systems. It refers to a series of experiments involved in a variety of biology, chemistry, medicine and other fields, including sample preprocessing, Operations such as sample reaction and result reading are integrated on a chip with micro-nano-sized structures. Among them, the centrifugal microfluidic chip refers to a type of chip that uses centrifugal force as power to drive samples or reagents to move in the microchannels of the chip to perform detection. In order to control the opening and closing of the liquid path on the centrifugal microfluidic chip, the related technology provides a microfluidic valve, which is activated by special structural design on the microfluidic chip, such as capillary, surface modification, film and other structures. To inhibit the flow of fluid, thereby realizing the opening and closing of the liquid path by controlling the opening and closing of the microfluidic valve.

然而，相关技术中的微流控阀门存在难以稳定开闭的问题。However, the microfluidic valve in the related art has a problem that it is difficult to open and close stably.

发明内容Contents of the invention

根据本申请的各种实施例，提供一种微流控芯片。According to various embodiments of the present application, a microfluidic chip is provided.

本申请提供了一种微流控芯片，包括：芯片本体，具有旋转中心轴；至少一个微流控阀门，所述微流控阀门包括特斯拉阀管路，所述特斯拉阀管路的相对两端分别具有进液口和出液口，所述进液口与所述旋转中心轴的距离小于所述出液口与所述旋转中心轴的距离，且所述特斯拉阀管路被配置为能够对从所述进液口向所述出液口流动的流体形成预设阻力；以及分别与所述微流控阀门一一对应的进液腔和反应腔，所述进液腔与对应的所述微流控阀门的所述特斯拉阀管路的所述进液口连通，所述反应腔与对应的所述微流控阀门的所述特斯拉阀管路的所述出液口连通。This application provides a microfluidic chip, including: a chip body with a central axis of rotation; at least one microfluidic valve, the microfluidic valve includes a Tesla valve pipeline, and the Tesla valve pipeline The opposite ends have a liquid inlet and a liquid outlet respectively, the distance between the liquid inlet and the rotation central axis is less than the distance between the liquid outlet and the rotation central axis, and the Tesla valve tube The path is configured to form a preset resistance to the fluid flowing from the liquid inlet to the liquid outlet; and there are liquid inlet chambers and reaction chambers respectively corresponding to the microfluidic valve, the liquid inlet The cavity is connected to the liquid inlet of the Tesla valve pipeline of the corresponding microfluidic valve, and the reaction chamber is connected to the Tesla valve pipeline of the corresponding microfluidic valve. The liquid outlet is connected.

在其中一个实施例中，所述特斯拉阀管路包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元组，所述特斯拉阀单元组包括至少一个特斯拉阀单元；In one embodiment, the Tesla valve pipeline includes a plurality of Tesla valve unit groups arranged along its extension direction and connected to each other. The Tesla valve unit group includes at least one Tesla valve unit. ;

每一所述特斯拉阀单元沿所述进液口指向所述出液口方向上的两端分别具有逆向进口端和逆向出口端，每一所述特斯拉阀单元被配置为能够阻挡流体从所述逆向进口端向所述逆向出口端流动，以使所述特斯拉阀管路被配置为能够对从所述进液口向所述出液口流动的流体形成预设阻力。Each Tesla valve unit has a reverse inlet end and a reverse outlet end respectively at both ends along the direction from the liquid inlet to the liquid outlet, and each Tesla valve unit is configured to be able to block Fluid flows from the reverse inlet end to the The flow is reversed to the outlet end, so that the Tesla valve pipeline is configured to form a preset resistance to the fluid flowing from the liquid inlet to the liquid outlet.

在其中一个实施例中，所述特斯拉阀单元包括第一通道和位于所述第一通道一侧的第二通道，所述第一通道的出口与所述第二通道的出口朝向彼此靠近的一侧延伸，并交汇形成汇合通道。In one embodiment, the Tesla valve unit includes a first channel and a second channel located on one side of the first channel, and the outlet of the first channel and the outlet of the second channel are close to each other. extend on one side and meet to form a confluence channel.

在其中一个实施例中，每一所述特斯拉阀单元组包括沿第一方向设置的至少两个所述特斯拉阀单元，同一所述特斯拉阀单元组中相邻的两个所述特斯拉阀单元彼此连通；In one embodiment, each Tesla valve unit group includes at least two Tesla valve units arranged along the first direction, and two adjacent Tesla valve units in the same Tesla valve unit group The Tesla valve units are connected to each other;

其中，所述第一方向与所述特斯拉阀管路的延伸方向相交设置。Wherein, the first direction intersects the extension direction of the Tesla valve pipeline.

在其中一个实施例中，同一所述特斯拉阀单元组中相邻的两个所述特斯拉阀单元沿所述第一方向交错布设。In one embodiment, two adjacent Tesla valve units in the same Tesla valve unit group are staggered along the first direction.

在其中一个实施例中，所述特斯拉阀管路包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元；In one embodiment, the Tesla valve pipeline includes a plurality of Tesla valve units arranged along its extension direction and connected with each other;

每一所述特斯拉阀单元包括彼此相对设置且沿所述进液口指向所述出液口方向上错位布设的两个管道，所述两个管道均具有沿所述进液口指向所述出液口方向上的第一端和第二端；Each of the Tesla valve units includes two pipes arranged opposite each other and arranged in a staggered direction along the liquid inlet toward the liquid outlet. Both pipes have a pipe pointing along the liquid inlet toward the liquid outlet. the first end and the second end in the direction of the liquid outlet;

所述两个管道包括第一管道和第二管道，第N个所述特斯拉阀单元的所述第一管道的所述第二端朝第N个所述特斯拉阀单元的所述第二管道延伸，并连接于第N+1个所述特斯拉阀单元的第一管道；第N个所述特斯拉阀单元的所述第二管道的所述第一端连接于第N个所述特斯拉阀单元的所述第一管道上位于第一端和所述第二端之间的部位，第N个所述特斯拉阀单元的所述第二管道的所述第二端朝第N+1个所述特斯拉阀单元的所述第一管道延伸，并连接于第N+1个所述特斯拉阀单元的第二管道上位于第一端和所述第二端之间的部位；第N+1个所述特斯拉阀单元的所述第一管道的所述第一端连接于第N个所述特斯拉阀单元的所述第二管道。The two pipes include a first pipe and a second pipe, and the second end of the first pipe of the Nth Tesla valve unit faces the Nth Tesla valve unit. The second pipe extends and is connected to the first pipe of the N+1th Tesla valve unit; the first end of the second pipe of the Nth Tesla valve unit is connected to the The portion of the first pipe of the N Tesla valve units located between the first end and the second end, the second pipe of the Nth Tesla valve unit The second end extends toward the first pipe of the N+1th Tesla valve unit and is connected to the second pipe of the N+1th Tesla valve unit between the first end and the second pipe of the Tesla valve unit. The position between the second ends; the first end of the first pipe of the N+1 Tesla valve unit is connected to the second end of the Nth Tesla valve unit pipeline.

在其中一个实施例中，第N个所述特斯拉阀单元的所述第一管道的第二端位于第N+1个所述特斯拉阀单元的所述第一管道的第一端和第N+1个所述特斯拉阀单元的所述第二管道的第一端之间；In one embodiment, the second end of the first pipe of the Nth Tesla valve unit is located at the first end of the first pipe of the N+1th Tesla valve unit. and the first end of the second pipe of the N+1 Tesla valve unit;

第N个所述特斯拉阀单元的所述第二管道的第二端位于第N+1个所述特斯拉阀单元的所述第二管道的第一端和第N+2个所述特斯拉阀单元的所述第一管道的第一端之间。The second end of the second pipe of the Nth Tesla valve unit is located between the first end of the second pipe of the N+1th Tesla valve unit and the N+2th Tesla valve unit. between the first end of the first pipe of the Tesla valve unit.

在其中一个实施例中，所述管道包括具有彼此连通的进液段和出液段，所述管道的所述第一端设置于所述进液段远离所述出液段的一端，所述管道的所述第二端设置于所述出液段远离所述进液段的一端，所述进液段被构造为呈直线状，所述出液段被构造为呈圆弧状。In one embodiment, the pipeline includes a liquid inlet section and a liquid outlet section that are connected to each other, and the first end of the pipeline is disposed at an end of the liquid inlet section away from the liquid outlet section, and the The second end of the pipe is disposed at an end of the liquid outlet section away from the liquid inlet section. The liquid inlet section is configured in a linear shape, and the liquid outlet section is configured in an arc shape.

在其中一个实施例中，所述微流控阀门沿所述芯片本体的径向延伸。In one embodiment, the microfluidic valve extends along the radial direction of the chip body.

在其中一个实施例中，所述微流控阀门的数量为多个； In one embodiment, the number of the microfluidic valves is multiple;

多个所述微流控阀门环绕所述旋转中心轴彼此间隔设置。A plurality of the microfluidic valves are spaced apart from each other around the central axis of rotation.

在其中一个实施例中，多个所述微流控阀门关于所述旋转中心轴旋转对称。In one embodiment, a plurality of the microfluidic valves are rotationally symmetrical about the central axis of rotation.

在其中一个实施例中，所述微流控芯片还包括分别与多个所述进液腔连通的至少一个分配管道，以及一一对应地与所述分配管道连通的加样舱，所述分配管道位于对应的所述加样仓远离所述旋转中心轴的一侧。In one embodiment, the microfluidic chip further includes at least one distribution pipe connected to a plurality of the liquid inlet chambers, and a sampling chamber connected to the distribution pipes in one-to-one correspondence. The pipeline is located on the side of the corresponding sampling chamber away from the central axis of rotation.

在其中一个实施例中，所述微流控芯片还包括与所述反应腔一一对应连通的虹吸阀。In one embodiment, the microfluidic chip further includes a siphon valve connected to the reaction chamber in a one-to-one correspondence.

在其中一个实施例中，所述微流控阀门包括毛细阀。In one embodiment, the microfluidic valve includes a capillary valve.

在其中一个实施例中，所述微流控阀门包括疏水阀。In one embodiment, the microfluidic valve includes a hydrophobic valve.

本申请的一个或多个实施例的细节在下面的附图和描述中提出。本申请的其他特征、目的和优点将从说明书、附图以及权利要求书变得明显。The details of one or more embodiments of the application are set forth in the accompanying drawings and the description below. Other features, objects and advantages of the application will become apparent from the description, drawings and claims.

附图说明Description of drawings

通过阅读对下文优选实施方式的详细描述，各种其他的优点和益处对于本领域普通技术人员将变得清楚明了。同时，为了更好地描述和说明这里公开的那些发明的实施例和/或示例，可以参考一幅或多幅附图。用于描述附图的附加细节或示例不应当被认为是对所公开的发明、目前描述的实施例和/或示例以及目前理解的这些发明的最佳模式中的任何一者的范围限制。Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. Meanwhile, in order to better describe and illustrate embodiments and/or examples of those inventions disclosed herein, reference may be made to one or more of the accompanying drawings. The additional details or examples used to describe the figures should not be construed as limiting the scope of any of the disclosed inventions, the embodiments and/or examples presently described, and the best modes currently understood of these inventions.

图1为根据一个或多个实施例提供的微流控芯片的结构示意图。Figure 1 is a schematic structural diagram of a microfluidic chip provided according to one or more embodiments.

图2为根据一个或多个实施例提供的微流控阀门、进液腔和反应腔的装配示意图。Figure 2 is a schematic assembly diagram of a microfluidic valve, a liquid inlet chamber and a reaction chamber provided according to one or more embodiments.

图3为根据一个或多个实施例提供的一个特斯拉阀单元的结构示意图。Figure 3 is a schematic structural diagram of a Tesla valve unit provided according to one or more embodiments.

图4为根据一个或多个实施例提供的第N至N+2个特斯拉阀单元的结构示意图。Figure 4 is a schematic structural diagram of the N to N+2 Tesla valve units provided according to one or more embodiments.

图5为根据一个或多个实施例提供的微流控阀门的结构示意图。Figure 5 is a schematic structural diagram of a microfluidic valve provided according to one or more embodiments.

图6为根据一个或多个实施例提供的一个特斯拉阀单元的结构示意图。Figure 6 is a schematic structural diagram of a Tesla valve unit provided according to one or more embodiments.

图7为根据一个或多个实施例提供的微流控阀门的结构示意图。Figure 7 is a schematic structural diagram of a microfluidic valve provided according to one or more embodiments.

图8为根据一个或多个实施例提供的微流控阀门的结构示意图。Figure 8 is a schematic structural diagram of a microfluidic valve provided according to one or more embodiments.

附图标记说明：100、微流控芯片；1、芯片本体；2、微流控阀门；20、特斯拉阀管路；21、进液口；22、出液口；23、特斯拉阀单元组；24、特斯拉阀单元；231、逆向进口端；232、逆向出口端；233、第一通道；234、第二通道；235、汇合通道；240、管道；241、第一管道；242、第二管道；2401、第一端；2402、第二端；2403、进液段；2404、出液段；30、进液腔；40、反应腔；50、分配管道；60、加样仓；70、虹吸阀；80、废液通道；A、第一方向。Explanation of reference signs: 100. Microfluidic chip; 1. Chip body; 2. Microfluidic valve; 20. Tesla valve pipeline; 21. Liquid inlet; 22. Liquid outlet; 23. Tesla Valve unit group; 24, Tesla valve unit; 231, reverse inlet end; 232, reverse outlet end; 233, first channel; 234, second channel; 235, converging channel; 240, pipeline; 241, first pipeline ; 242. Second pipe; 2401. First end; 2402. Second end; 2403. Liquid inlet section; 2404. Liquid outlet section; 30. Liquid inlet chamber; 40. Reaction chamber; 50. Distribution pipe; 60. Adding Sample bin; 70. Siphon valve; 80. Waste liquid channel; A. First direction.

具体实施方式 Detailed ways

下面将结合附图对本申请技术方案的实施例进行详细的描述。以下实施例仅用于更加清楚地说明本申请的技术方案，因此只作为示例，而不能以此来限制本申请的保护范围。The embodiments of the technical solution of the present application will be described in detail below with reference to the accompanying drawings. The following examples are only used to illustrate the technical solution of the present application more clearly, and are therefore only used as examples and cannot be used to limit the protection scope of the present application.

除非另有定义，本文所使用的所有的技术和科学术语与属于本申请的技术领域的技术人员通常理解的含义相同；本文中所使用的术语只是为了描述具体的实施例的目的，不是旨在于限制本申请；本申请的说明书和权利要求书及上述附图说明中的术语“包括”和“具有”以及它们的任何变形，意图在于覆盖不排他的包含。Unless otherwise defined, all technical and scientific terms used herein have the same meanings as commonly understood by those skilled in the technical field belonging to this application; the terms used herein are for the purpose of describing specific embodiments only and are not intended to be used in Limitation of this application; the terms "including" and "having" and any variations thereof in the description and claims of this application and the above description of the drawings are intended to cover non-exclusive inclusion.

在本申请实施例的描述中，技术术语“第一”“第二”等仅用于区别不同对象，而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量、特定顺序或主次关系。在本申请实施例的描述中，“多个”的含义是两个以上，除非另有明确具体的限定。In the description of the embodiments of this application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying the relative importance or implicitly indicating the quantity or specificity of the indicated technical features. Sequence or priority relationship. In the description of the embodiments of this application, "plurality" means two or more, unless otherwise explicitly and specifically limited.

在本文中提及“实施例”意味着，结合实施例描述的特定特征、结构或特性可以包含在本申请的至少一个实施例中。在说明书中的各个位置出现该短语并不一定均是指相同的实施例，也不是与其它实施例互斥的独立的或备选的实施例。本领域技术人员显式地和隐式地理解的是，本文所描述的实施例可以与其它实施例相结合。Reference herein to "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of this phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those skilled in the art understand, both explicitly and implicitly, that the embodiments described herein may be combined with other embodiments.

在本申请实施例的描述中，术语“和/或”仅仅是一种描述关联对象的关联关系，表示可以存在三种关系，例如A和/或B，可以表示：单独存在A，同时存在A和B，单独存在B这三种情况。另外，本文中字符“/”，一般表示前后关联对象是一种“或”的关系。In the description of the embodiments of this application, the term "and/or" is only an association relationship describing associated objects, indicating that there can be three relationships, such as A and/or B, which can mean: A exists alone, and A exists simultaneously and B, there are three cases of B alone. In addition, the character "/" in this article generally indicates that the related objects are an "or" relationship.

在本申请实施例的描述中，术语“多个”指的是两个以上(包括两个)，同理，“多组”指的是两组以上(包括两组)，“多片”指的是两片以上(包括两片)。In the description of the embodiments of this application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to two or more groups (including two groups), and "multiple pieces" refers to It is more than two pieces (including two pieces).

在本申请实施例的描述中，技术术语“中心”“纵向”“横向”“长度”“宽度”“厚度”“上”“下”“前”“后”“左”“右”“竖直”“水平”“顶”“底”“内”“外”“顺时针”“逆时针”“轴向”“径向”“周向”等指示的方位或位置关系为基于附图所示的方位或位置关系，仅是为了便于描述本申请实施例和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本申请实施例的限制。In the description of the embodiments of this application, the technical terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "back", "left", "right" and "vertical" The orientation or positional relationships indicated by "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. are based on those shown in the accompanying drawings. The orientation or positional relationship is only for the convenience of describing the embodiments of the present application and simplifying the description. It does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the implementation of the present application. Example limitations.

在本申请实施例的描述中，除非另有明确的规定和限定，技术术语“安装”“相连”“连接”“固定”等术语应做广义理解，例如，可以是固定连接，也可以是可拆卸连接，或成一体；也可以是机械连接，也可以是电连接；可以是直接相连，也可以通过中间媒介间接相连，可以是两个元件内部的连通或两个元件的相互作用关系。对于本领域的普通技术人员而言，可以根据具体情况理解上述术语在本申请实施例中的具体含义。In the description of the embodiments of this application, unless otherwise clearly stated and limited, technical terms such as "installation", "connection", "connection" and "fixing" should be understood in a broad sense. For example, it can be a fixed connection or a removable connection. It can be disassembled and connected, or integrated; it can also be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be an internal connection between two elements or an interaction between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of this application can be understood according to specific circumstances.

本申请一实施例提供了一种微流控芯片100，如图1和图2所示，微流控芯片100包括芯片本体1、至少一个微流控阀门2和分别与微流控阀门2一一对应的进液腔30和反应腔40。芯片本体1具有旋转中心轴(图未示)，微流控阀门2包括特斯拉阀管路20，特斯拉阀管路20的相对两端分别具有进液口21和出液口22，进液口21与旋转中心轴的距离小于出液口22与旋转中心轴的距离，且特斯拉阀管路20被配置为能够对从进液口21向出液口22 流动的流体形成预设阻力。进液腔30与对应的微流控阀门2的特斯拉阀管路20的进液口21连通，反应腔40与对应的微流控阀门2的特斯拉阀管路20的出液口22连通。An embodiment of the present application provides a microfluidic chip 100. As shown in Figures 1 and 2, the microfluidic chip 100 includes a chip body 1, at least one microfluidic valve 2 and a microfluidic valve 2 respectively. A corresponding liquid inlet chamber 30 and reaction chamber 40. The chip body 1 has a central axis of rotation (not shown). The microfluidic valve 2 includes a Tesla valve pipeline 20. The opposite ends of the Tesla valve pipeline 20 have a liquid inlet 21 and a liquid outlet 22 respectively. The distance between the liquid inlet 21 and the central axis of rotation is smaller than the distance between the liquid outlet 22 and the central axis of rotation, and the Tesla valve pipeline 20 is configured to be able to flow from the liquid inlet 21 to the liquid outlet 22 The flowing fluid creates a preset resistance. The liquid inlet chamber 30 is connected to the liquid inlet 21 of the corresponding Tesla valve pipeline 20 of the microfluidic valve 2, and the reaction chamber 40 is connected to the liquid outlet of the corresponding Tesla valve pipeline 20 of the microfluidic valve 2. 22 connected.

上述微流控芯片100，通过设置微流控阀门2包括特斯拉阀管路20，特斯拉阀管路20靠近旋转中心轴的一端具有进液口21，特斯拉阀管路20远离旋转中心轴的一端具有出液口22，使当芯片本体1绕旋转中心轴转动时，流体在离心力的作用下的流向为从进液口21向出液口22流动。而由于特斯拉阀管路20被配置为能够对进液口21向出液口22流动的流体形成预设阻力，从而使芯片本体1需要以较高的转速绕旋转中心轴旋转，使流体受到的离心力大于预设阻力，才能使进液口21的流体克服预设阻力流动至出液口22，即，使微流控阀门2打开。而当流体受到的离心力小于预设阻力时，流体无法从进液口21流至出液口22，微流控阀门2关闭。因此，能够通过改变芯片本体1绕旋转中心轴转动的转速，改变离心力的大小，实现微流控阀门2的打开或关闭，并使得微流控阀门2能分别在一定的转速范围内稳定地保持打开状态或关闭状态。The above-mentioned microfluidic chip 100 includes a Tesla valve pipeline 20 by setting a microfluidic valve 2. The Tesla valve pipeline 20 has a liquid inlet 21 at one end close to the central axis of rotation, and the Tesla valve pipeline 20 is far away from the central axis of rotation. One end of the central axis of rotation has a liquid outlet 22, so that when the chip body 1 rotates around the central axis of rotation, the flow direction of the fluid under the action of centrifugal force is from the liquid inlet 21 to the liquid outlet 22. Since the Tesla valve pipeline 20 is configured to form a preset resistance to the fluid flowing from the liquid inlet 21 to the liquid outlet 22, the chip body 1 needs to rotate around the central axis of rotation at a higher speed, causing the fluid to Only when the centrifugal force is greater than the preset resistance can the fluid in the liquid inlet 21 overcome the preset resistance and flow to the liquid outlet 22, that is, the microfluidic valve 2 can be opened. When the centrifugal force experienced by the fluid is less than the preset resistance, the fluid cannot flow from the liquid inlet 21 to the liquid outlet 22, and the microfluidic valve 2 is closed. Therefore, by changing the rotation speed of the chip body 1 around the central axis of rotation and changing the size of the centrifugal force, the microfluidic valve 2 can be opened or closed, and the microfluidic valve 2 can be stably maintained within a certain rotational speed range. Open state or closed state.

在实际使用过程中，先将试剂加入进液腔30，再通过控制芯片本体1绕旋转中心轴的转速，控制微流控阀门2的打开或关闭，即控制试剂能否进入反应腔40内。因此，上述实施例中的微流控芯片100，能够更精确地控制芯片本体1上试剂的加入顺序和反应步骤，控制孵育时间，从而实现复杂的多步化学反应。In actual use, the reagent is first added into the liquid chamber 30, and then the opening or closing of the microfluidic valve 2 is controlled by controlling the rotation speed of the chip body 1 around the central axis of rotation, that is, whether the reagent can enter the reaction chamber 40 is controlled. Therefore, the microfluidic chip 100 in the above embodiment can more accurately control the adding sequence and reaction steps of reagents on the chip body 1 and control the incubation time, thereby realizing complex multi-step chemical reactions.

基于不同结构的特斯拉阀管路20提供如下实施例：The following embodiments are provided based on Tesla valve pipeline 20 with different structures:

在一些实施例中，如图2和图3所示，特斯拉阀管路20包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元24，每一特斯拉阀单元24包括彼此相对设置且沿进液口21指向出液口22方向上错位布设的两个管道240，两个管道240均具有沿进液口21指向出液口22方向上的第一端2401和第二端2402。如图4所示，两个管道240包括第一管道241和第二管道242，第N个特斯拉阀单元24的第一管道241的第二端2412朝第N个特斯拉阀单元24的第二管道242延伸，并连接于第N+1个特斯拉阀单元24的第一管道241。第N个特斯拉阀单元24的第二管道242的第一端2421连接于第N个特斯拉阀单元24的第一管道241上位于第一端2411和第二端2412之间的部位，第N个特斯拉阀单元24的第二管道242的第二端2422朝第N+1个特斯拉阀单元24的第一管道241延伸，并连接于第N+1个特斯拉阀单元24的第二管道242上位于第一端2421和第二端2422之间的部位。第N+1个特斯拉阀单元24的第一管道241的第一端2411连接于第N个特斯拉阀单元24的第二管道242。In some embodiments, as shown in FIGS. 2 and 3 , the Tesla valve pipeline 20 includes a plurality of Tesla valve units 24 arranged along its extension direction and connected to each other. Each Tesla valve unit 24 includes Two pipes 240 are arranged opposite each other and are arranged in a staggered direction along the direction from the liquid inlet 21 to the liquid outlet 22. Both pipes 240 have a first end 2401 and a second end along the direction from the liquid inlet 21 toward the liquid outlet 22. Terminal 2402. As shown in FIG. 4 , the two pipes 240 include a first pipe 241 and a second pipe 242 , and the second end 2412 of the first pipe 241 of the Nth Tesla valve unit 24 faces the Nth Tesla valve unit 24 The second pipe 242 extends and is connected to the first pipe 241 of the N+1 Tesla valve unit 24. The first end 2421 of the second pipe 242 of the Nth Tesla valve unit 24 is connected to a portion of the first pipe 241 of the Nth Tesla valve unit 24 between the first end 2411 and the second end 2412 , the second end 2422 of the second pipe 242 of the Nth Tesla valve unit 24 extends toward the first pipe 241 of the N+1th Tesla valve unit 24 and is connected to the N+1th Tesla valve unit 24. The portion of the second pipe 242 of the valve unit 24 is located between the first end 2421 and the second end 2422 . The first end 2411 of the first pipe 241 of the N+1 Tesla valve unit 24 is connected to the second pipe 242 of the Nth Tesla valve unit 24 .

如此，上述实施例中通过设置每一特斯拉阀单元24包括两个管道240，且两个管道240均具有沿进液口21指向出液口22方向上的第一端2401和第二端2402(见图3)，使当芯片本体1绕旋转中心轴转动时，流体的流向为从每一管道240的第一端2401向同一管道240的第二端2402流动。通过设置第N个特斯拉阀单元24的第一管道241的第二端2412朝第N个特斯拉阀单元24的第二管道242延伸，使在流体从进液口21向出液口22流动时，流动至第N个特斯拉阀单元24的第一管道241的第二端2412内的流体的流速，与流动至第N个特斯拉阀单元24的第二管道242内的流体的流速不同向。并且由于设置第N+1个特斯拉阀单元24的第一管道241的第一端2411与第N个特斯拉阀单元24的第二管道242连接，使当流体从进液口21向出液口22流动时，进入第N个特斯拉阀单元24的第二管道242的流体一部分从同一第二管道242的第二端2402流出，另一部分进入第N+1个特斯拉阀单元24的第一管道241的第一端2401。又由于第N个特斯拉阀单元24的第一管道241的第二端2412与第N+1个特斯拉阀单元24的第一管道241连接，使在第N个特斯拉阀单元24的第一管道241的第二端2412与第N+1个特斯拉阀单元24的第一管道241的连接处相遇的流体的流速反向，从而使第N个特斯拉阀单元24的第一管道241的第二端2412流出的流体与第N+1个特斯拉阀单元24的第一管道241内的流体相遇时，对第N+1个特斯拉阀单元24的第一管道241内的流体起到阻挡流动的作用。同样地，第N个特斯拉阀单元24的第二管道242的第二端2422流出的流体与第N+1个特斯拉阀单元24的第二管道242内的流体相遇时，对第N+1个特斯拉阀单元24的第二管道242内的流体起到阻挡流动的作用。因此，通过设置特斯拉阀管路20包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元24，使当流体从第N个特斯拉阀单元24流进第N+1个特斯拉阀单元24时，第N个特斯拉阀单元24与第N+1个特斯拉阀单元24的连接处对流体形成阻力，从而使特斯拉阀管路20对从进液口21向出液口22流动的流体形成预设阻力。In this way, in the above embodiment, each Tesla valve unit 24 includes two pipes 240 , and both pipes 240 have a first end 2401 and a second end along the liquid inlet 21 pointing toward the liquid outlet 22 . 2402 (see Figure 3), so that when the chip body 1 rotates around the central axis of rotation, the flow direction of the fluid is from the first end 2401 of each pipe 240 to the second end 2402 of the same pipe 240. By arranging the second end 2412 of the first pipe 241 of the Nth Tesla valve unit 24 to extend toward the second pipe 242 of the Nth Tesla valve unit 24, the fluid flows from the liquid inlet 21 to the liquid outlet. When 22 flows, The flow rate of the fluid flowing into the second end 2412 of the first pipe 241 of the Nth Tesla valve unit 24 is different from the flow rate of the fluid flowing into the second pipe 242 of the Nth Tesla valve unit 24 Towards. And since the first end 2411 of the first pipe 241 of the N+1 Tesla valve unit 24 is connected to the second pipe 242 of the Nth Tesla valve unit 24, when the fluid flows from the liquid inlet 21 to When the liquid outlet 22 flows, part of the fluid entering the second pipe 242 of the Nth Tesla valve unit 24 flows out from the second end 2402 of the same second pipe 242, and the other part enters the N+1th Tesla valve. First end 2401 of first conduit 241 of unit 24. And because the second end 2412 of the first pipe 241 of the Nth Tesla valve unit 24 is connected to the first pipe 241 of the N+1th Tesla valve unit 24, so that in the Nth Tesla valve unit The flow rate of the fluid that meets the second end 2412 of the first pipe 241 of 24 and the first pipe 241 of the N+1 Tesla valve unit 24 is reversed, so that the Nth Tesla valve unit 24 When the fluid flowing out of the second end 2412 of the first pipe 241 meets the fluid in the first pipe 241 of the N+1 Tesla valve unit 24, the fluid flowing out of the N+1 Tesla valve unit 24 will The fluid in a pipe 241 acts as a flow barrier. Similarly, when the fluid flowing out of the second end 2422 of the second pipe 242 of the Nth Tesla valve unit 24 meets the fluid in the second pipe 242 of the N+1th Tesla valve unit 24, the The fluid in the second pipe 242 of the N+1 Tesla valve units 24 acts to block the flow. Therefore, by arranging the Tesla valve pipeline 20 to include a plurality of Tesla valve units 24 arranged along its extension direction and connected to each other, when the fluid flows from the Nth Tesla valve unit 24 into the N+1th When the Tesla valve unit 24 is installed, the connection between the Nth Tesla valve unit 24 and the N+1th Tesla valve unit 24 forms a resistance to the fluid, thereby causing the Tesla valve pipeline 20 to pass through the liquid inlet. The fluid flowing from the port 21 to the liquid outlet 22 forms a preset resistance.

在一些实施例中，N为正整数。In some embodiments, N is a positive integer.

需要说明的是，两股流体的流速同向，指两股流体的运动方向相同，两股流体的流速反向，指两股流体的运动方向相反。It should be noted that the flow rates of the two fluids are in the same direction, which means that the two fluids move in the same direction, and the flow rates of the two fluids are in the opposite direction, which means that the two fluids move in opposite directions.

在一些实施例中，如图4所示，第N个特斯拉阀单元24的第一管道241的第二端2402位于第N+1个特斯拉阀单元24的第一管道241的第一端2401和第N+1个特斯拉阀单元24的第二管道242的第一端2401之间。第N个特斯拉阀单元24的第二管道242的第二端2402位于第N+1个特斯拉阀单元24的第二管道242的第一端2401和第N+2个特斯拉阀单元24的第一管道241的第一端2401之间。如此，通过设置第N个特斯拉阀单元24的第一管道241的第二端2402位于第N+1个特斯拉阀单元24的第一管道241的第一端2401和第N+1个特斯拉阀单元24的第二管道242的第一端2401之间，使当流体流进第N+1个特斯拉阀单元24的第一管道241的第一端2401后，就受到从第N个特斯拉阀单元24的第一管道241的第二端2402中流出的流体的阻挡。即，在第N+1个特斯拉阀单元24的第一管道241内的流体分流进第N+1个特斯拉阀单元24的第二管道242之前，第N+1个特斯拉阀单元24的第一管道241内的流体的流速降低，从而降低了分流进第N+1个特斯拉阀单元24的第二管道242内的流体的流速，进一步提高了特斯拉阀管路20对从进液口21向出液口22流动的流体形成的预设阻力。 In some embodiments, as shown in FIG. 4 , the second end 2402 of the first pipe 241 of the Nth Tesla valve unit 24 is located at the first pipe 241 of the N+1th Tesla valve unit 24 . Between one end 2401 and the first end 2401 of the second pipe 242 of the N+1 Tesla valve unit 24. The second end 2402 of the second pipe 242 of the Nth Tesla valve unit 24 is located at the first end 2401 of the second pipe 242 of the N+1th Tesla valve unit 24 and the N+2th Tesla between the first end 2401 of the first pipe 241 of the valve unit 24 . In this way, by arranging the second end 2402 of the first pipe 241 of the Nth Tesla valve unit 24 to be located at the first end 2401 of the first pipe 241 of the N+1th Tesla valve unit 24 and the N+1th between the first ends 2401 of the second pipes 242 of the N+1 Tesla valve units 24, so that when the fluid flows into the first end 2401 of the first pipe 241 of the N+1 Tesla valve unit 24, it is Blocking of fluid flowing out from the second end 2402 of the first pipe 241 of the Nth Tesla valve unit 24 . That is, before the fluid in the first pipe 241 of the N+1th Tesla valve unit 24 flows into the second pipe 242 of the N+1th Tesla valve unit 24, the N+1th Tesla valve The flow rate of the fluid in the first pipe 241 of the pull valve unit 24 is reduced, thereby reducing the flow rate of the fluid branched into the second pipe 242 of the N+1th Tesla valve unit 24, further increasing the Tesla The valve pipeline 20 forms a preset resistance to the fluid flowing from the liquid inlet 21 to the liquid outlet 22 .

可以理解的是，当特斯拉阀管路20对从进液口21向出液口22流动的流体形成的预设阻力提高，则需要较大的离心力才能使流体从进液口21流动至出液口22，因此，当芯片本体1绕旋转中心轴转动的转速大于与预设阻力对应的临界转速时，微流控阀门2开启，当芯片本体1绕旋转中心轴转动的转速大于与预设阻力对应的临界转速时，微流控阀门2打开。由此可知，上述实施例中，通过提高特斯拉阀管路20对从进液口21向出液口22流动的流体形成的预设阻力，也就提高了芯片本体1在高转速条件下工作时，微流控阀门2关闭的稳定性和可靠性。It can be understood that when the preset resistance formed by the Tesla valve pipeline 20 to the fluid flowing from the liquid inlet 21 to the liquid outlet 22 increases, a larger centrifugal force is required to make the fluid flow from the liquid inlet 21 to the liquid outlet 22 . Liquid outlet 22. Therefore, when the rotation speed of the chip body 1 around the central axis of rotation is greater than the critical rotation speed corresponding to the preset resistance, the microfluidic valve 2 opens. When the rotation speed of the chip body 1 around the central axis of rotation is greater than the preset resistance, When the critical speed corresponding to the resistance is set, the microfluidic valve 2 is opened. It can be seen from this that in the above embodiment, by increasing the preset resistance of the Tesla valve pipeline 20 to the fluid flowing from the liquid inlet 21 to the liquid outlet 22, the performance of the chip body 1 under high-speed conditions is also improved. When working, the microfluidic valve 2 is closed with stability and reliability.

其中，临界转速为使微流控阀门2打开时，芯片本体1绕旋转中心轴转动的转速值。The critical rotation speed is the rotation speed value at which the chip body 1 rotates around the central axis of rotation when the microfluidic valve 2 is opened.

在一些实施例中，如图2-4所示，管道240包括具有彼此连通的进液段2403和出液段2404，管道240的第一端2401设置于进液段2403远离出液段2404的一端，管道240的第二端2402设置于出液段2404远离进液段2403的一端，进液段2403被构造为呈直线状，出液段2404被构造为呈圆弧状。如此，通过设置出液段2404呈圆弧状，使同一管道240的出液段2404的延伸方向与进液段2403的延伸方向不同，从而使第一管道241的第二端2412能够朝同一特斯拉阀单元24的第二管道242延伸。In some embodiments, as shown in Figures 2-4, the pipeline 240 includes a liquid inlet section 2403 and a liquid outlet section 2404 that are connected to each other. The first end 2401 of the pipeline 240 is disposed on the liquid inlet section 2403 away from the liquid outlet section 2404. At one end, the second end 2402 of the pipe 240 is disposed at an end of the liquid outlet section 2404 away from the liquid inlet section 2403. The liquid inlet section 2403 is configured in a straight line, and the liquid outlet section 2404 is structured in an arc shape. In this way, by arranging the liquid outlet section 2404 in an arc shape, the extending direction of the liquid outlet section 2404 of the same pipe 240 is different from the extending direction of the liquid inlet section 2403, so that the second end 2412 of the first pipe 241 can move toward the same feature. The second conduit 242 of the Sla valve unit 24 extends.

在一些实施例中，如图5和图6所示，特斯拉阀管路20包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元组23，特斯拉阀单元组23包括至少一个特斯拉阀单元24。每一特斯拉阀单元24沿进液口21指向出液口22方向上的两端分别具有逆向进口端231和逆向出口端232，每一特斯拉阀单元24被配置为能够阻挡流体从逆向进口端231向逆向出口端232流动，以使特斯拉阀管路20被配置为能够对从进液口21向出液口22流动的流体形成预设阻力。如此，使流体从进液口21向出液口22流动时，流体流经多个特斯拉阀单元组23，而当流体流经每一特斯拉阀单元组23时，流体从特斯拉阀单元组23的特斯拉阀单元24的逆向进口端231向逆向出口端232流动，从而受到特斯拉阀单元24形成的阻挡作用，使流体的流速降低。而由于流体从进液口21流进特斯拉阀管路20后，需要经过多组特斯拉阀单元组23才能流至出液口22，从而使特斯拉阀管路20对流体形成较大的预设阻力，使芯片本体1在高转速条件下工作时，微流控阀门2能够稳定可靠地关闭。In some embodiments, as shown in FIGS. 5 and 6 , the Tesla valve pipeline 20 includes a plurality of Tesla valve unit groups 23 arranged along its extension direction and connected to each other. The Tesla valve unit group 23 includes At least one Tesla valve unit 24. Each Tesla valve unit 24 has a reverse inlet end 231 and a reverse outlet end 232 at both ends along the direction from the liquid inlet 21 to the liquid outlet 22. Each Tesla valve unit 24 is configured to block fluid from flowing out. The reverse inlet end 231 flows toward the reverse outlet end 232 , so that the Tesla valve pipeline 20 is configured to form a preset resistance to the fluid flowing from the liquid inlet 21 to the liquid outlet 22 . In this way, when the fluid flows from the liquid inlet 21 to the liquid outlet 22, the fluid flows through multiple Tesla valve unit groups 23, and when the fluid flows through each Tesla valve unit group 23, the fluid flows from the Tesla valve unit group 23 to the liquid outlet 22. The reverse inlet end 231 of the Tesla valve unit 24 of the pull valve unit group 23 flows toward the reverse outlet end 232, thereby being blocked by the Tesla valve unit 24 and reducing the flow rate of the fluid. Since the fluid flows from the liquid inlet 21 into the Tesla valve pipeline 20, it needs to pass through multiple groups of Tesla valve unit groups 23 before it can flow to the liquid outlet 22, so that the Tesla valve pipeline 20 forms a barrier for the fluid. The larger preset resistance enables the microfluidic valve 2 to be closed stably and reliably when the chip body 1 is working under high-speed conditions.

此外，上述实施例中通过设置特斯拉阀管路20包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元组23，还使得第一次使流体通过特斯拉阀管路20后，残留在特斯拉阀管路20内的流体在离心力作用下沉积到特斯拉阀管路20的靠近出液口22的一段。此时，特斯拉阀管路20靠近进液口21的一段内保持通畅，因此特斯拉阀管路20内未形成闭塞的液滴，从而使微流控阀门2的两端的气体***，微流控阀门2的两端的气压平衡。如此，当需要第二次使流体通过微流控阀门2时，使微流控阀门2打开的离心力与上一次的离心力相同，因此，使多次打开微流控阀门2的离心力保持恒定，从而便于微流控阀门2的重复使用，能够进行多步化学反应。例如，在实际使用过程中，可以将不同种类的试剂按照实验反应需要的顺序加入进液腔30内，并在每次向进液腔30内加入一种或多种试剂后，使微流控阀门2打开，从而使不同种类的试剂能够按照实验反应需要的顺序进入反应体系，控制孵育时间，以精确控制液流的次序及反应过程，提高微流控芯片100中化学反应的复杂程度，拓展微流控芯片100中化学反应的类别。In addition, in the above embodiment, by arranging the Tesla valve pipeline 20 to include a plurality of Tesla valve unit groups 23 arranged along its extension direction and connected to each other, it is also possible to allow fluid to pass through the Tesla valve pipeline 20 for the first time. Finally, the fluid remaining in the Tesla valve pipeline 20 is deposited to a section of the Tesla valve pipeline 20 close to the liquid outlet 22 under the action of centrifugal force. At this time, the section of the Tesla valve pipeline 20 close to the liquid inlet 21 remains unobstructed, so no occluded droplets are formed in the Tesla valve pipeline 20 , thereby allowing the gas at both ends of the microfluidic valve 2 to move freely. , the air pressure at both ends of the microfluidic valve 2 is balanced. In this way, when the fluid needs to pass through the microfluidic valve 2 for the second time, the centrifugal force to open the microfluidic valve 2 is the same as the centrifugal force of the last time. Therefore, the centrifugal force to open the microfluidic valve 2 multiple times remains constant, thus It is convenient to reuse the microfluidic valve 2 and can carry out multi-step chemical reactions. For example, in actual use, different types of reagents can be used according to experimental reaction needs The microfluidic valve 2 is opened after adding one or more reagents into the liquid inlet chamber 30 each time, so that different types of reagents can enter in the order required for the experimental reaction. The reaction system controls the incubation time to accurately control the order of liquid flow and the reaction process, thereby increasing the complexity of chemical reactions in the microfluidic chip 100 and expanding the types of chemical reactions in the microfluidic chip 100 .

在一些实施例中，如图6所示，特斯拉阀单元24包括第一通道233和位于第一通道233一侧的第二通道234，第一通道233的出口与第二通道234的出口朝向彼此靠近的一侧延伸，并交汇形成汇合通道235。如此，使从第一通道233的出口流出的流体的流速与从第二通道234的出口流出的流体的流速反向，从而分别降低了从第一通道233的出口流出的流体的流速，以及从第二通道234的出口流出的流体的流速，因此使流体从逆向进口端231指向逆向出口端232的方向流过每一特斯拉阀单元24时，均受到阻挡作用，使流体的流速降低。In some embodiments, as shown in Figure 6, the Tesla valve unit 24 includes a first channel 233 and a second channel 234 located on one side of the first channel 233. The outlet of the first channel 233 and the outlet of the second channel 234 They extend toward the sides closer to each other and meet to form a merging channel 235 . In this way, the flow rate of the fluid flowing out from the outlet of the first channel 233 and the flow rate of the fluid flowing out from the outlet of the second channel 234 are reversed, thereby respectively reducing the flow rate of the fluid flowing out from the outlet of the first channel 233, and from The flow rate of the fluid flowing out of the outlet of the second channel 234 is therefore blocked when the fluid flows through each Tesla valve unit 24 in the direction from the reverse inlet end 231 to the reverse outlet end 232, reducing the flow rate of the fluid.

在一些实施例中，如图7和图8所示，每一特斯拉阀单元组23包括沿第一方向设置的至少两个特斯拉阀单元24，同一特斯拉阀单元组23中相邻的两个特斯拉阀单元24彼此连通。其中，第一方向与特斯拉阀管路20的延伸方向相交设置。如此，通过设置每一特斯拉阀单元组23包括沿第一方向设置的至少两个特斯拉阀单元24，使特斯拉阀管路20中相邻组的特斯拉阀单元24的连接处的数量增加，使得当流体从进液口21指向出液口22的方向流经特斯拉阀管路20时，流体所经过的分叉口和汇合口的数量增加。需要说明，当流体在流道内流动时，其前端的流道面积突然增大或减小都会产生阻力，因此，当流体经过分叉口和汇合口时产生较大的液阻。因此，通过设置每一特斯拉阀单元组23包括沿第一方向设置的至少两个特斯拉阀单元24，使流体通过交错排列的多个分叉口和汇合口，从而进一步提高了特斯拉阀管路20对从进液口21向出液口22流动的流体形成的预设阻力，进一步提高了芯片本体1在高转速条件下工作时，微流控阀门2关闭的稳定性和可靠性。In some embodiments, as shown in FIGS. 7 and 8 , each Tesla valve unit group 23 includes at least two Tesla valve units 24 arranged along the first direction. In the same Tesla valve unit group 23 Two adjacent Tesla valve units 24 are connected to each other. The first direction intersects the extending direction of the Tesla valve pipeline 20 . In this way, by arranging each Tesla valve unit group 23 to include at least two Tesla valve units 24 arranged along the first direction, the adjacent groups of Tesla valve units 24 in the Tesla valve pipeline 20 are The number of connections increases, so that when the fluid flows through the Tesla valve pipeline 20 from the liquid inlet 21 to the liquid outlet 22, the number of bifurcated openings and converging openings that the fluid passes through increases. It should be noted that when the fluid flows in the flow channel, a sudden increase or decrease in the flow channel area at the front end will produce resistance. Therefore, when the fluid passes through the bifurcation port and the confluence port, a large liquid resistance is generated. Therefore, by arranging each Tesla valve unit group 23 to include at least two Tesla valve units 24 arranged along the first direction, the fluid can pass through a plurality of staggered bifurcation ports and merging ports, thereby further improving the characteristics. The preset resistance formed by the Sla valve pipeline 20 on the fluid flowing from the liquid inlet 21 to the liquid outlet 22 further improves the stability and stability of the closing of the microfluidic valve 2 when the chip body 1 operates under high-speed conditions. reliability.

在一些实施例中，如图7和图8所示，同一特斯拉阀单元组23中相邻的两个特斯拉阀单元24沿第一方向交错布设，以使流体从一特斯拉阀单元组23中的特斯拉阀单元24流进相邻特斯拉阀单元组23中的特斯拉阀单元24时，流体前端的流道面积能够突然增大或减小。In some embodiments, as shown in FIGS. 7 and 8 , two adjacent Tesla valve units 24 in the same Tesla valve unit group 23 are staggered along the first direction, so that the fluid flows from one Tesla to When the Tesla valve unit 24 in the valve unit group 23 flows into the Tesla valve unit 24 in the adjacent Tesla valve unit group 23, the flow channel area at the front end of the fluid can suddenly increase or decrease.

在一些实施例中，如图5和图7-8所示，每一特斯拉阀单元组23包括的特斯拉阀单元24的数量可以是1个～3个。另一些实施例中，每一特斯拉阀单元组23包括的特斯拉阀单元24的数量也可以根据使用需要设置，只要能满足对特斯拉阀管路20对从进液口21向出液口22流动的流体形成的预设阻力大小的要求即可，在此不做限定。In some embodiments, as shown in FIG. 5 and FIGS. 7-8 , the number of Tesla valve units 24 included in each Tesla valve unit group 23 may be 1 to 3. In other embodiments, the number of Tesla valve units 24 included in each Tesla valve unit group 23 can also be set according to usage needs, as long as it can meet the requirements for the Tesla valve pipeline 20 from the liquid inlet 21 to The preset resistance level formed by the fluid flowing through the liquid outlet 22 is sufficient and is not limited here.

在一些实施例中，微流控阀门2包括毛细阀。In some embodiments, microfluidic valve 2 includes a capillary valve.

在另一些实施例中，微流控阀门2包括疏水阀。需要说明的是，当微流控阀门2采用疏水阀时，对微流控阀门2的表面进行疏水改性。由于微流控阀门2中流道的高度会直接影响微流控阀门2的阻力，因此当需要设置打开微流控阀门2的临界转速极高时，对应的流道的高度极低，导致加工难度加大，此时通过进行疏水改性，能够适当增加微流控阀门2的流道的高度，降低加工难度。In other embodiments, the microfluidic valve 2 includes a hydrophobic valve. It should be noted that when the microfluidic valve 2 adopts a hydrophobic valve, the surface of the microfluidic valve 2 is hydrophobically modified. Since the height of the flow channel in the microfluidic valve 2 will directly affect the resistance of the microfluidic valve 2, when the critical speed for opening the microfluidic valve 2 needs to be set to be extremely high, the height of the corresponding flow channel will be extremely low, resulting in difficulty in processing. increase, at this time, by performing hydrophobic modification, the flow rate of the microfluidic valve 2 can be appropriately increased. The height of the channel reduces the difficulty of processing.

基于图7所示实施例中提供的微流控阀门2，表一为在给定的圆心距位置上，进行测试，得到的与不同特斯拉阀管路20的高度对应的临界转速。其中，临界转速使微流控阀门2打开时，芯片本体1绕旋转中心轴转动的转速值。为了便于陈述，给定圆心距为旋转中心轴到特斯拉阀管路20的进液口21之间的距离，为3厘米。芯片本体1的材质为PMMA(polymethyl methacrylate聚甲基丙烯酸甲酯)，芯片本体1的厚度为1mm，不代表微流控阀门2只能在圆心距3厘米的空间上排布，亦不代表微流控阀门2只能在PMMA上使用，亦不代表芯片厚度只能为1mm。Based on the microfluidic valve 2 provided in the embodiment shown in FIG. 7 , Table 1 shows the critical rotation speed corresponding to different heights of the Tesla valve pipeline 20 obtained through testing at a given circle center distance position. Among them, the critical speed is the speed value at which the chip body 1 rotates around the central axis of rotation when the microfluidic valve 2 is opened. For the convenience of description, the given circle center distance is the distance between the central axis of rotation and the liquid inlet 21 of the Tesla valve pipeline 20, which is 3 cm. The material of the chip body 1 is PMMA (polymethyl methacrylate), and the thickness of the chip body 1 is 1 mm. This does not mean that the microfluidic valves 2 can only be arranged in a space with a center distance of 3 cm, nor does it mean that the microfluidic valves 2 can only be arranged in a space with a center distance of 3 cm. Fluid control valve 2 can only be used on PMMA, which does not mean that the chip thickness can only be 1mm.

表一
Table I

基于图7所示实施例中提供的微流控阀门2，表二为给定特斯拉阀管路20的高度，进行测试，得到的与不同的圆心距对应的临界转速。为了便于陈述，其中，给定特斯拉阀管路20的高度为100微米，芯片本体1的材质为PMMA，芯片本体1的厚度为1mm。Based on the microfluidic valve 2 provided in the embodiment shown in Figure 7, Table 2 shows the critical rotation speeds corresponding to different center distances obtained by testing given the height of the Tesla valve pipeline 20. For ease of description, it is assumed that the height of the Tesla valve pipeline 20 is 100 microns, the material of the chip body 1 is PMMA, and the thickness of the chip body 1 is 1 mm.

表二
Table II

基于图7所示实施例中提供的微流控阀门2，表三为给定给定圆心距和特斯拉阀管路20的高度，对六个微流控阀门2测试其稳定性。为了便于陈述，其中，圆心距为3厘米，特斯拉阀管路20的高度为100微米，芯片本体1的材质为PMMA，芯片本体1的厚度为1mm。 Based on the microfluidic valve 2 provided in the embodiment shown in FIG. 7 , Table 3 shows the stability of six microfluidic valves 2 given a given circle center distance and the height of the Tesla valve pipeline 20 . For the convenience of description, the center distance of the circles is 3 cm, the height of the Tesla valve pipeline 20 is 100 microns, the material of the chip body 1 is PMMA, and the thickness of the chip body 1 is 1 mm.

表三
Table 3

由表三可知，上述实施例中微流控阀门2具有较高的稳定性，即能够分别在不同的转速下保持稳定关闭或稳定打开。As can be seen from Table 3, the microfluidic valve 2 in the above embodiment has high stability, that is, it can remain stably closed or stably opened at different rotational speeds.

在一些实施例中，如图1所示，微流控阀门2的数量为多个，多个微流控阀门2环绕旋转中心轴彼此间隔设置。如此，能够将作为不同样品的试剂分别放入与多个微流控阀门2对应的多个进液腔30内，使当芯片本体1绕旋转中心轴转动时，能够实现多样品的检测。In some embodiments, as shown in FIG. 1 , the number of microfluidic valves 2 is multiple, and the plurality of microfluidic valves 2 are spaced apart from each other around the central axis of rotation. In this way, reagents serving as different samples can be put into multiple liquid inlet chambers 30 corresponding to multiple microfluidic valves 2, so that when the chip body 1 rotates around the central axis of rotation, multiple samples can be detected.

在一些实施例中，多个微流控阀门2关于旋转中心轴旋转对称，从而当芯片本体1绕旋转中心轴转动时，彼此对称的微流控阀门2同步打开或关闭，使芯片本体1上的对称单元执行相同操作。In some embodiments, multiple microfluidic valves 2 are rotationally symmetrical about the central axis of rotation, so that when the chip body 1 rotates around the central axis of rotation, the symmetrical microfluidic valves 2 open or close synchronously, causing the chip body 1 to The same operation is performed for symmetrical units.

在一些实施例中，如图1所示，微流控阀门2沿芯片本体1的径向延伸，以便于更好地利用芯片本体1上的空间。另一些实施例中，微流控阀门2的延伸方向也可以与芯片本体1的径向交叉设置，只要使特斯拉阀管路20的进液口21与旋转中心轴的距离小于特斯拉阀管路20的出液口22与旋转中心轴的距离即可，在此不做限定。In some embodiments, as shown in FIG. 1 , the microfluidic valve 2 extends along the radial direction of the chip body 1 to better utilize the space on the chip body 1 . In other embodiments, the extension direction of the microfluidic valve 2 can also be arranged across the radial direction of the chip body 1 , as long as the distance between the liquid inlet 21 of the Tesla valve pipeline 20 and the central axis of rotation is smaller than the distance between the Tesla valve pipeline 20 and the central axis of rotation. The distance between the liquid outlet 22 of the valve pipeline 20 and the central axis of rotation is sufficient and is not limited here.

在一些实施例中，如图1所示，微流控阀门2的数量为多个，微流控芯片100还包括分别与多个进液腔30连通的至少一个分配管道50，以及一一对应地与分配管道50连通的加样仓60，分配通道位于对应的加样仓60远离旋转中心轴的一侧。如此，通过设置加样仓60，以加入试剂，并使试剂通过分配管道50进入对应的进液腔30内。In some embodiments, as shown in FIG. 1 , the number of microfluidic valves 2 is multiple, and the microfluidic chip 100 also includes at least one distribution pipe 50 connected to multiple liquid inlet chambers 30 respectively, and a one-to-one correspondence. The distribution channel is located on the side of the corresponding sampling chamber 60 away from the central axis of rotation. In this way, the sample adding chamber 60 is provided to add reagents, and the reagents enter the corresponding liquid inlet chamber 30 through the distribution pipe 50 .

在一些实施例中，连接于同一分配通道的多个进液腔30的容积相同。In some embodiments, the volumes of multiple liquid inlet chambers 30 connected to the same distribution channel are the same.

在一些实施例中，如图1所示，微流控芯片100还包括与反应腔40一一对应连通的虹吸阀70，以使反应腔40内的流体能够在芯片本体1停转后，在毛细力作用下通过虹吸阀70流出反应腔40。In some embodiments, as shown in FIG. 1 , the microfluidic chip 100 also includes a siphon valve 70 that communicates with the reaction chamber 40 in a one-to-one correspondence, so that the fluid in the reaction chamber 40 can be released after the chip body 1 stops rotating. It flows out of the reaction chamber 40 through the siphon valve 70 under the action of capillary force.

在一些实施例中，如图1所示，微流控芯片100还包括与分配通道一一对应的废液通道80，废液通道80与对应的反应腔40连通，以收容完成反应后从反应腔40经过虹吸阀70流出反应腔40的流体。In some embodiments, as shown in FIG. 1 , the microfluidic chip 100 also includes a waste liquid channel 80 corresponding to the distribution channel. The waste liquid channel 80 is connected with the corresponding reaction chamber 40 to accommodate the reaction chamber 40 after the reaction is completed. Fluid from the reaction chamber 40 flows out of the chamber 40 through the siphon valve 70 .

基于上述实施例中提供的微流控芯片100，提供一种微流控芯片100的使用方法：Based on the microfluidic chip 100 provided in the above embodiment, a method of using the microfluidic chip 100 is provided:

S110：将磁珠及酶冻干球预置于进液腔30内，将样本血浆与配套稀释液稀释混匀后加入到加样仓60，加样量为100-500ul；S110: Pre-place the magnetic beads and enzyme lyophilized beads in the liquid inlet chamber 30, dilute and mix the sample plasma with the supporting diluent and then add it to the sample addition chamber 60, with a sample addition volume of 100-500ul;

S120：使芯片本体1以小于临界转速的第一预设转速绕旋转中心轴转动第一预设转动时间； S120: Rotate the chip body 1 around the central axis of rotation for a first preset rotation time at a first preset rotation speed that is less than the critical rotation speed;

如此，使加样仓60内的流体在离心力作用下通过分配通道，填满进液腔30。In this way, the fluid in the sampling chamber 60 passes through the distribution channel under the action of centrifugal force and fills the liquid inlet chamber 30 .

在一些实施例中，临界转速为1200rpm～2000rpm，第一预设转速为500rpm～1000rpm，第一预设转动时间为20s～60s。In some embodiments, the critical rotation speed is 1200rpm~2000rpm, the first preset rotation speed is 500rpm~1000rpm, and the first preset rotation time is 20s~60s.

S130：使芯片本体1以大于临界转速的第二预设转速绕旋转中心轴转动第二预设转动时间；S130: Rotate the chip body 1 around the central axis of rotation at a second preset speed greater than the critical speed for a second preset rotation time;

如此，使微流控阀门2打开，进液腔30内的流体通过微流控阀门2流进对应的反应腔40内，开始反应。In this way, the microfluidic valve 2 is opened, and the fluid in the liquid inlet chamber 30 flows into the corresponding reaction chamber 40 through the microfluidic valve 2 to start the reaction.

在一些实施例中，第二预设转速为1500rpm。In some embodiments, the second preset rotation speed is 1500 rpm.

S140：使芯片本体1绕旋转中心轴转动第三预设转动时间，且芯片本体1的转速在第一预设转速区间内交替变化。S140: Rotate the chip body 1 around the central axis of rotation for a third preset rotation time, and the rotation speed of the chip body 1 alternately changes within the first preset rotation range.

如此，促进冻干球溶解及混匀操作。In this way, the dissolving and mixing operations of the freeze-dried beads are promoted.

在一些实施例中，第一预设转速区间为1000rpm～3000rpm，第三预设转动时间为10分钟～15分钟。In some embodiments, the first preset rotation speed range is 1000rpm~3000rpm, and the third preset rotation time is 10 minutes~15 minutes.

S150：使芯片本体1停止转动，且芯片本体1停止转动的时长为第一预设停转时间。S150: Stop the rotation of the chip body 1, and the duration for which the chip body 1 stops rotating is the first preset stop time.

如此，使流体在毛细力作用下通过虹吸阀70。In this way, the fluid passes through the siphon valve 70 under the action of capillary force.

在一些实施例中，第一预设停转时间为10s～30s。In some embodiments, the first preset stall time is 10s˜30s.

S160：使芯片本体1以第一预设转速绕旋转中心轴转动20s，使流体进入废液通道80。S160: Rotate the chip body 1 around the central axis of rotation at the first preset speed for 20 seconds to allow the fluid to enter the waste liquid channel 80 .

在一些实施例中，微流控芯片100的使用方法还包括：In some embodiments, the method of using the microfluidic chip 100 further includes:

将清洗液加入加样仓60，加样量为100-500ul，使芯片本体1以第一预设转速绕旋转中心轴转动20s～60s，使清洗液填满进液腔30。再使芯片本体1以第二预设转速绕旋转中心轴转动，使清洗液进入反应腔40，进行交替变加速顺时针旋转，芯片本体1的转速在第一预设转速区间内交替变化，持续时间为3分钟。停止10-30s，使清洗液在毛细力作用下通过虹吸阀70。使芯片本体1以第一预设转速绕旋转中心轴转动20s，使液体到达废液通道80。重复2-3次。Add the cleaning liquid to the sample addition chamber 60 with a sample volume of 100-500 ul. Rotate the chip body 1 around the central axis of rotation at the first preset speed for 20s to 60s, so that the cleaning liquid fills the liquid inlet chamber 30 . Then, the chip body 1 is rotated around the central axis of rotation at a second preset speed, so that the cleaning liquid enters the reaction chamber 40 and rotates clockwise with alternating acceleration. The speed of the chip body 1 alternately changes within the first preset speed range, and continues The time is 3 minutes. Stop for 10-30 seconds to allow the cleaning fluid to pass through the siphon valve 70 under the action of capillary force. The chip body 1 is rotated around the central axis of rotation at the first preset speed for 20 seconds, so that the liquid reaches the waste liquid channel 80 . Repeat 2-3 times.

添加底物至加样仓60，加样量为100-500ul。使芯片本体1以第一预设转速绕旋转中心轴转动20s～60s，使底物通过分配管道50，填满进液腔30。再使芯片本体1以第二预设转速绕旋转中心轴转动，使底物进入反应腔40，在此进行交替变加速顺时针旋转，转速在1分钟内维持1000-3000rpm交替变化，持续时间为3分钟。停止5分钟后进行检测。Add substrate to the sample chamber 60, and the sample volume is 100-500ul. The chip body 1 is rotated around the central axis of rotation at a first preset speed for 20 to 60 seconds, so that the substrate passes through the distribution pipe 50 and fills the liquid inlet chamber 30 . Then, the chip body 1 is rotated around the central axis of rotation at a second preset speed, so that the substrate enters the reaction chamber 40, where it alternately accelerates and rotates clockwise. The rotation speed maintains an alternating change of 1000-3000 rpm within 1 minute, and the duration is 3 minutes. Test after stopping for 5 minutes.

以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。The technical features of the above-described embodiments can be combined in any way. To simplify the description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, All should be considered to be within the scope of this manual.

以上所述实施例仅表达了本申请的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对申请专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本申请构思的前提下，还可以做出若干变形和改进，这些都属于本申请的保护范围。因此，本申请专利的保护范围应以所附权利要求为准。 The above-described embodiments only express several implementation modes of the present application, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the patent application. It should be noted that, for those of ordinary skill in the art, in Without departing from the concept of this application, several deformations and improvements can be made, which all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

一种微流控芯片(100)，其中，包括：A microfluidic chip (100), which includes:

芯片本体(1)，具有旋转中心轴；The chip body (1) has a central axis of rotation;

至少一个微流控阀门(2)，所述微流控阀门(2)包括特斯拉阀管路(20)，所述特斯拉阀管路(20)的相对两端分别具有进液口(21)和出液口(22)，所述进液口(21)与所述旋转中心轴的距离小于所述出液口(22)与所述旋转中心轴的距离，且所述特斯拉阀管路(20)被配置为能够对从所述进液口(21)向所述出液口(22)流动的流体形成预设阻力；以及At least one microfluidic valve (2). The microfluidic valve (2) includes a Tesla valve pipeline (20). Opposite ends of the Tesla valve pipeline (20) have liquid inlets respectively. (21) and a liquid outlet (22), the distance between the liquid inlet (21) and the central axis of rotation is smaller than the distance between the liquid outlet (22) and the central axis of rotation, and the Tes The pull valve pipeline (20) is configured to form a preset resistance to the fluid flowing from the liquid inlet (21) to the liquid outlet (22); and

分别与所述微流控阀门(2)一一对应的进液腔(30)和反应腔(40)，所述进液腔(30)与对应的所述微流控阀门(2)的所述特斯拉阀管路(20)的所述进液口(21)连通，所述反应腔(40)与对应的所述微流控阀门(2)的所述特斯拉阀管路(20)的所述出液口(22)连通。The liquid inlet chamber (30) and the reaction chamber (40) respectively correspond to the microfluidic valve (2) one-to-one, and the liquid inlet chamber (30) and the corresponding microfluidic valve (2) all have The liquid inlet (21) of the Tesla valve pipeline (20) is connected, and the reaction chamber (40) is connected to the Tesla valve pipeline (2) of the corresponding microfluidic valve (2). The liquid outlet (22) of 20) is connected.
根据权利要求1所述的微流控芯片(100)，其中，所述特斯拉阀管路(20)包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元组(23)，所述特斯拉阀单元组(23)包括至少一个特斯拉阀单元(24)；The microfluidic chip (100) according to claim 1, wherein the Tesla valve pipeline (20) includes a plurality of Tesla valve unit groups (23) arranged along its extension direction and connected to each other, The Tesla valve unit group (23) includes at least one Tesla valve unit (24);

每一所述特斯拉阀单元(24)沿所述进液口(21)指向所述出液口(22)方向上的两端分别具有逆向进口端(231)和逆向出口端(232)，每一所述特斯拉阀单元(24)被配置为能够阻挡流体从所述逆向进口端(231)向所述逆向出口端(232)流动，以使所述特斯拉阀管路(20)被配置为能够对从所述进液口(21)向所述出液口(22)流动的流体形成预设阻力。Each Tesla valve unit (24) has a reverse inlet end (231) and a reverse outlet end (232) at both ends along the direction from the liquid inlet (21) to the liquid outlet (22). , each Tesla valve unit (24) is configured to block the flow of fluid from the reverse inlet end (231) to the reverse outlet end (232), so that the Tesla valve pipeline (232) 20) is configured to form a preset resistance to the fluid flowing from the liquid inlet (21) to the liquid outlet (22).
根据权利要求2所述的微流控芯片(100)，其中，所述特斯拉阀单元(24)包括第一通道(233)和位于所述第一通道(233)一侧的第二通道(234)，所述第一通道(233)的出口与所述第二通道(234)的出口朝向彼此靠近的一侧延伸，并交汇形成汇合通道(235)。The microfluidic chip (100) according to claim 2, wherein the Tesla valve unit (24) includes a first channel (233) and a second channel located on one side of the first channel (233) (234), the outlet of the first channel (233) and the outlet of the second channel (234) extend toward the side closer to each other, and meet to form a merging channel (235).
根据权利要求3所述的微流控芯片(100)，其中，每一所述特斯拉阀单元组(23)包括沿第一方向设置的至少两个所述特斯拉阀单元(24)，同一所述特斯拉阀单元组(23)中相邻的两个所述特斯拉阀单元(24)彼此连通；The microfluidic chip (100) according to claim 3, wherein each Tesla valve unit group (23) includes at least two Tesla valve units (24) arranged along a first direction. , two adjacent Tesla valve units (24) in the same Tesla valve unit group (23) are connected to each other;

其中，所述第一方向与所述特斯拉阀管路(20)的延伸方向相交设置。Wherein, the first direction intersects the extension direction of the Tesla valve pipeline (20).
根据权利要求4所述的微流控芯片(100)，其中，同一所述特斯拉阀单元组(23)中相邻的两个所述特斯拉阀单元(24)沿所述第一方向交错布设。The microfluidic chip (100) according to claim 4, wherein two adjacent Tesla valve units (24) in the same Tesla valve unit group (23) are located along the first The directions are staggered.
根据权利要求1所述的微流控芯片(100)，其中，所述特斯拉阀管路(20)包括沿其延伸方向布设并彼此连通的多个特斯拉阀单元(24)；The microfluidic chip (100) according to claim 1, wherein the Tesla valve pipeline (20) includes a plurality of Tesla valve units (24) arranged along its extension direction and connected to each other;

每一所述特斯拉阀单元(24)包括彼此相对设置且沿所述进液口(21)指向所述出液口(22)方向上错位布设的两个管道(240)，所述两个管道(240)均具有沿所述进液口(21)指向所述出液口(22)方向上的第一端(2401)和第二端(2402)；Each Tesla valve unit (24) includes two pipes (240) arranged opposite each other and arranged staggered in the direction from the liquid inlet (21) to the liquid outlet (22). Each pipe (240) has a first end (2401) and a second end (2402) pointing in the direction of the liquid inlet (21) toward the liquid outlet (22);

所述两个管道(240)包括第一管道(241)和第二管道(242)，第N个所述特斯拉阀单元(24)的所述第一管道(241)的所述第二端(2402)朝第N个所述特斯拉阀单元(24)的所述第二管道(242)延伸，并连接于第N+1个所述特斯拉阀单元(24)的第一管道(241)；第N个所述特斯拉阀单元(24) 的所述第二管道(242)的所述第一端(2401)连接于第N个所述特斯拉阀单元(24)的所述第一管道(241)上位于第一端(2401)和所述第二端(2402)之间的部位，第N个所述特斯拉阀单元(24)的所述第二管道(242)的所述第二端(2402)朝第N+1个所述特斯拉阀单元(24)的所述第一管道(241)延伸，并连接于第N+1个所述特斯拉阀单元(24)的第二管道(242)上位于第一端(2401)和所述第二端(2402)之间的部位；第N+1个所述特斯拉阀单元(24)的所述第一管道(241)的所述第一端(2401)连接于第N个所述特斯拉阀单元(24)的所述第二管道(242)。The two pipes (240) include a first pipe (241) and a second pipe (242), and the second pipe (241) of the Nth Tesla valve unit (24) The end (2402) extends toward the second pipe (242) of the Nth Tesla valve unit (24) and is connected to the first pipe (242) of the N+1th Tesla valve unit (24). Pipeline (241); Nth Tesla Valve Unit (24) The first end (2401) of the second pipe (242) is connected to the first pipe (241) of the Nth Tesla valve unit (24) and is located at the first end (2401) and the second end (2402), the second end (2402) of the second pipe (242) of the Nth Tesla valve unit (24) faces the N+1 The first pipe (241) of the Tesla valve unit (24) extends and is connected to the second pipe (242) of the N+1 Tesla valve unit (24) located at the The position between one end (2401) and the second end (2402); the first end (241) of the first pipe (241) of the N+1 Tesla valve unit (24) 2401) The second pipe (242) connected to the Nth Tesla valve unit (24).
根据权利要求6所述的微流控芯片(100)，其中，第N个所述特斯拉阀单元(24)的所述第一管道(241)的第二端(2402)位于第N+1个所述特斯拉阀单元(24)的所述第一管道(241)的第一端(2401)和第N+1个所述特斯拉阀单元(24)的所述第二管道(242)的第一端(2401)之间；The microfluidic chip (100) according to claim 6, wherein the second end (2402) of the first pipe (241) of the Nth Tesla valve unit (24) is located at the N+th The first end (2401) of the first pipe (241) of one Tesla valve unit (24) and the second pipe of the N+1 Tesla valve unit (24) between the first ends (2401) of (242);

第N个所述特斯拉阀单元(24)的所述第二管道(242)的第二端(2402)位于第N+1个所述特斯拉阀单元(24)的所述第二管道(242)的第一端(2401)和第N+2个所述特斯拉阀单元(24)的所述第一管道(241)的第一端(2401)之间。The second end (2402) of the second pipe (242) of the Nth Tesla valve unit (24) is located at the second end of the N+1th Tesla valve unit (24). Between the first end (2401) of the pipe (242) and the first end (2401) of the first pipe (241) of the N+2 Tesla valve unit (24).
根据权利要求6所述的微流控芯片(100)，其中，所述管道(240)包括具有彼此连通的进液段(2403)和出液段(2404)，所述管道(240)的所述第一端(2401)设置于所述进液段(2403)远离所述出液段(2404)的一端，所述管道(240)的所述第二端(2402)设置于所述出液段(2404)远离所述进液段(2403)的一端，所述进液段(2403)被构造为呈直线状，所述出液段(2404)被构造为呈圆弧状。The microfluidic chip (100) according to claim 6, wherein the pipeline (240) includes a liquid inlet section (2403) and a liquid outlet section (2404) that are connected to each other, and all parts of the pipeline (240) The first end (2401) is disposed at an end of the liquid inlet section (2403) away from the liquid outlet section (2404), and the second end (2402) of the pipe (240) is disposed at the liquid outlet section. One end of the section (2404) is away from the liquid inlet section (2403), the liquid inlet section (2403) is configured in a straight line, and the liquid outlet section (2404) is constructed in an arc shape.
根据权利要求1至8任一项所述的微流控芯片(100)，其中，所述微流控阀门(2)沿所述芯片本体(1)的径向延伸。The microfluidic chip (100) according to any one of claims 1 to 8, wherein the microfluidic valve (2) extends along the radial direction of the chip body (1).
根据权利要求1至9任一项所述的微流控芯片(100)，其中，所述微流控阀门(2)的数量为多个；The microfluidic chip (100) according to any one of claims 1 to 9, wherein the number of the microfluidic valves (2) is multiple;

多个所述微流控阀门(2)环绕所述旋转中心轴彼此间隔设置。A plurality of the microfluidic valves (2) are spaced apart from each other around the central axis of rotation.
根据权利要求10所述的微流控芯片(100)，其中，多个所述微流控阀门(2)关于所述旋转中心轴旋转对称。The microfluidic chip (100) according to claim 10, wherein a plurality of the microfluidic valves (2) are rotationally symmetrical about the central axis of rotation.
根据权利要求10所述的微流控芯片(100)，其中，所述微流控芯片(100)还包括分别与多个所述进液腔(30)连通的至少一个分配管道(50)，以及一一对应地与所述分配管道(50)连通的加样仓(60)，所述分配管道(50)位于对应的所述加样仓(60)远离所述旋转中心轴的一侧。The microfluidic chip (100) according to claim 10, wherein the microfluidic chip (100) further includes at least one distribution pipe (50) connected to a plurality of the liquid inlet chambers (30) respectively, And a sampling chamber (60) connected to the distribution pipe (50) in a one-to-one correspondence, and the distribution pipe (50) is located on the side of the corresponding sampling chamber (60) away from the central axis of rotation.
根据权利要求1-12任一项所述的微流控芯片(100)，其中，所述微流控芯片(100)还包括与所述反应腔(40)一一对应连通的虹吸阀(70)。The microfluidic chip (100) according to any one of claims 1-12, wherein the microfluidic chip (100) further includes a siphon valve (70) connected to the reaction chamber (40) in one-to-one correspondence ).
根据权利要求1-13任一项所述的微流控芯片(100)，其中，所述微流控阀门(2)包括毛细阀。The microfluidic chip (100) according to any one of claims 1-13, wherein the microfluidic valve (2) includes a capillary valve.
根据权利要求1-14任一项所述的微流控芯片(100)，其中，所述微流控阀门(2)包括疏水阀。 The microfluidic chip (100) according to any one of claims 1-14, wherein the microfluidic valve (2) includes a hydrophobic valve.