WO2023273903A1 - Dietetic invigoration product capable of rapidly supplementing nutrients by nucleotide and preparation method therefor, and detection method for nucleotide in dietetic invigoration product - Google Patents

Dietetic invigoration product capable of rapidly supplementing nutrients by nucleotide and preparation method therefor, and detection method for nucleotide in dietetic invigoration product Download PDF

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Publication number
WO2023273903A1
WO2023273903A1 PCT/CN2022/099207 CN2022099207W WO2023273903A1 WO 2023273903 A1 WO2023273903 A1 WO 2023273903A1 CN 2022099207 W CN2022099207 W CN 2022099207W WO 2023273903 A1 WO2023273903 A1 WO 2023273903A1
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Prior art keywords
nucleotide
abalone
nucleotides
parts
product
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PCT/CN2022/099207
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French (fr)
Chinese (zh)
Inventor
戴晨义
戴晨旭
董庆盈
李淑洁
金煜彬
Original Assignee
百珍堂生物科技(浙江)有限公司
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Priority claimed from CN202110746475.5A external-priority patent/CN113552251A/en
Priority claimed from CN202110819983.1A external-priority patent/CN113519807A/en
Application filed by 百珍堂生物科技(浙江)有限公司 filed Critical 百珍堂生物科技(浙江)有限公司
Publication of WO2023273903A1 publication Critical patent/WO2023273903A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00—Soups; Sauces; Preparation or treatment thereof
    • A23L23/10—Soup concentrates, e.g. powders or cakes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Definitions

  • the invention relates to the technical field of food processing and the technical field of nutrient component detection, and in particular to a food supplement product for rapid nutritional supplementation with nucleotides, a preparation method thereof and a detection method for nucleotides in the food supplement product.
  • Immunity is the body's own defense mechanism. It is the body's ability to recognize and eliminate any foreign bodies invaded by the outside world, to deal with aging, damaged, dead, and denatured self-cells, and to recognize and deal with mutant cells and virus-infected cells in the body. Eliminate the physiological reactions of "other people”. With the accelerated pace of life, high life pressure, irregular diet, improper medication, irregular life and other reasons, the immunity of some people has decreased, which seriously affects human health. When the immune function of the human body is low, the body presents a state of non-resistance, and the human body is very easy to cause bacteria, viruses, etc., and is easy to get sick, especially the concurrent infection of influenza, which is very serious.
  • Nucleotides are important low-molecular compounds in living organisms and have many physiological functions. In addition to being the precursors of DNA and RNA, they are also involved in the metabolism of substances in the body as regulators of physiological and biochemical processes. Nucleotides can enhance immunity, enhance iron absorption to help brain development and so on.
  • Abalone is a single-shell mollusc, which is a traditional Chinese precious food material. It ranks first among the four oceans. It is delicious in meat and rich in nutrition. It is rich in amino acid phospholipids, glycolipids, fatty acids, sterols, nucleic acids, and colorants Nutrients also contain Cu, Fe, Zn and other elements, which can improve human immunity and inhibit the growth of bacteria and viruses. Therefore, it is of great significance to improve the health status of immunocompromised people by using abalone as the main raw material, scientifically matching formula, researching and developing safe, effective and nutritious food supplement products with nucleotides for rapid nutritional supplementation that can enhance immunity.
  • Nucleotides are a class of compounds composed of purine or pyrimidine bases, ribose or deoxyribose, and phosphoric acid, including: CMP (cytosine nucleotide), UMP (uracil nucleotide), AMP (adenine Nucleotides), GMP (guanine nucleotides) and IMP (inosine nucleotides) five nucleotides.
  • CMP cytosine nucleotide
  • UMP uracil nucleotide
  • AMP adenine Nucleotides
  • GMP guanine nucleotides
  • IMP inosine nucleotides
  • the present invention provides a food tonic product for rapid nutritional supplementation of nucleotides and a preparation method thereof to solve the above-mentioned technical problems.
  • the present invention provides following technical scheme:
  • a food tonic product for rapid nutritional supplementation of nucleotides including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 parts of abalone broth
  • the auxiliary materials include the following raw materials in parts by mass: curry chicken oil 45 ⁇ 53 parts, 8 ⁇ 12 parts of sweet butter, 3 ⁇ 4 parts of edible salt, 1 ⁇ 2 parts of red bowl monosodium glutamate, 8.5 ⁇ 9.5 parts of chicken powder, 50 ⁇ 58 parts of starch, 1 ⁇ 2 parts of edible alkali , 12 ⁇ 15 parts of dark soy sauce, 45 ⁇ 52 parts of oil consumption, 0.8 ⁇ 1.2 parts of disodium I+G flavored nucleotide, 1 ⁇ 2 parts of red yeast rice water.
  • the main ingredients include the following raw materials by mass: 1000 parts of abalone stock;
  • the auxiliary materials include the following raw materials by mass: 50 parts of chicken curry oil, 10 parts of sweet butter, 3.125 parts of edible salt, and 1.2 parts of red bowl monosodium glutamate , 9.3 parts of chicken powder, 55 parts of starch, 1.25 parts of edible alkali, 14 parts of dark soy sauce, 50 parts of fuel consumption, 1 part of disodium I+G flavored nucleotide, and 1.5 parts of red yeast rice water.
  • Another object of the present invention is to provide a method for preparing food tonic products for rapid nutritional supplementation of nucleotides, which includes the following steps in sequence: raw material acceptance—thawing of main ingredients—cleaning—blanching—soup extraction—pressing—filtration—precipitation— Excipient pretreatment - blending - homogenization - heat exchange - filling - labeling - quick freezing and refrigeration - high temperature sterilization - metal detection - packaging inspection, the whole process is aseptic production.
  • the raw material acceptance method is as follows: take an appropriate amount of raw materials and place them in a clean white plate, observe the color and state under natural light, and smell the smell. It is required that the raw materials have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision.
  • the preparation method of the food tonic product of said nucleotide rapid nutritional supplement specifically comprises the following steps:
  • Step (1) raw material acceptance
  • Step (2) thawing of main ingredients: place the qualified abalone in a clean environment to thaw in a natural state;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: place the cleaned abalone in hot steam or hot water, and cook for 30 to 60 minutes at a temperature of 85°C to 100°C to remove foreign matter such as blood residue; Take out the abalone, remove the abalone meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: add abalone meat to cold water, then heat and boil to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into the blending tank, add evenly mixed auxiliary materials, boil and continue heating for 100 minutes to 150 minutes, and keep warm at 45°C ⁇ 55°C for 30 minutes to 50 minutes after the end, get thick broth;
  • Step (9) homogenization: transfer the thick soup prepared in step (8) into a homogenizer through pipelines for emulsification and homogenization, so that it can be emulsified finely and dispersed evenly;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 45°C ⁇ 60°C, and then pump out the air The pressure tank is sealed and labeled to obtain a semi-finished product;
  • Step (12) quick-freezing and refrigerating: quick-freezing the filled and packaged semi-finished product for 1.5 hours to 3 hours with a quick-freezing machine, and then refrigerating at a low temperature for later use;
  • Step (13) high-temperature sterilization: high-temperature sterilization of the quick-frozen and refrigerated semi-finished products
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • the heating and boiling conditions are 1 atm to 2 atm and 100° C. to 120° C. for 1 hour to 3 hours.
  • the emulsification and homogenization conditions are as follows: the temperature is 65° C. to 85° C., the pressure is 10 MPa to 20 MPa, and the time is 25 minutes to 35 minutes.
  • the conditions of the high-temperature sterilization treatment are: the sterilization temperature is 126° C. to 131° C., and the time is 12 minutes to 32 minutes.
  • the key limits of the detection accuracy of the metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the present invention provides a method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements.
  • the technical solution adopted by the present invention to solve the above problems is: a method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements, the nucleotides are cytosine nucleotides, adenine nucleotides, uracil nucleotides Nucleotide, guanine nucleotide and inosine nucleotide five kinds of nucleotides;
  • the detection method comprises the following steps:
  • Step 1 Supply five kinds of nuclei containing nucleotides as cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine nucleotide to the high performance liquid chromatography respectively.
  • Step 2 Take a food supplement product sample frozen at -60°C based on nucleotide rapid nutritional supplementation, thaw at room temperature, take an appropriate amount of sample in a 10mLEP tube, put it in a high-speed centrifuge at 10,000r/min for 10min, and pass through a 0.22um micropore After filtering the membrane, put it into a 1.5mL autosampler bottle, store it in a refrigerator at 4°C, and take it out before analysis;
  • step three the sample is supplied to high performance liquid chromatography to obtain the concentration of nucleotides.
  • step 1 the working parameters of the high performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; Sample volume: 10 ⁇ l; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
  • the working parameters of the high performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; Sample volume: 10 ⁇ l; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
  • the food tonic product for rapid nutritional supplementation of nucleotides of the present invention uses abalone as the main ingredient, rationally screens the raw materials of auxiliary materials, and cooperates with specific formula ratios, so that the prepared food tonic product has a balanced taste, strong aroma, and low flavor. It is full-bodied and rich in nutrients such as amino acids, nucleotides, and proteins. It can not only meet consumers' needs in terms of taste, but also support the improvement of human immunity and meet consumers' needs in terms of nutrition and health.
  • the food tonic product for rapid nutritional supplementation of nucleotides of the present invention uses abalone as a raw material, boils a thick broth, and by controlling the temperature and time, the nutritional components and taste of the abalone are fully retained to the greatest extent; by using a specific proportion of The raw materials of auxiliary materials and the coordination between raw materials of auxiliary materials are conducive to improving the flavor of food supplement products and improving the effect of food supplement products on enhancing human immunity.
  • the invention boils the raw material broth under certain conditions, which ensures the full extraction and dissolution of the nutritional components in the main ingredients, and ensures the nutritional value and flavor of the product; the use of a homogenizer ensures the homogenization of the dispersed particles in the broth system Homogenization and homogenization, reducing the size of the dispersion and improving the uniformity of distribution; the soup dregs pressing system is used to squeeze out the solid residue after cooking, making full use of raw materials, so that the nutritional components in the product are more complete and more Rich, higher concentration; the invention adopts the cold chain quick-freezing process, which retains the deliciousness of the soup to the greatest extent, and at the same time obtains good preservation and stability.
  • the preparation method of the present invention is simple, and the product is complete in color, fragrance and taste, and is suitable for industrial production.
  • the prepared food tonic product for rapid nutritional supplementation of nucleotides is rich in nutrition, has no peculiar fishy smell, has high nucleotide content, is delicious in taste, is convenient to use, and can meet the needs of consumers.
  • the present invention adopts the external standard method to calibrate the high performance liquid chromatography, and the food tonic product to be tested can be directly analyzed by high performance liquid chromatography, the sample does not need to be processed, the operation is simple, the sample consumption is small, the sensitivity is high, and the specificity is strong , which can be used in food supplement products based on nucleotide rapid nutritional supplements.
  • Fig. 1 is the process flow chart of the food tonic product of nucleotide rapid nutritional supplement of the present invention.
  • Fig. 2 is the test report 1 of the food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
  • Fig. 3 is the detection report 2 of the food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
  • Fig. 4 is a test report 3 of a food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
  • Fig. 5 is a test report 1 of a food tonic product sample prepared in Comparative Example 1 of the present invention for rapid nutritional supplementation with nucleotides.
  • Fig. 6 is the detection report 2 of the food tonic product sample prepared in comparative example 1 of the present invention for nucleotide rapid nutritional supplementation.
  • Fig. 7 is the detection report 3 of the food tonic product sample prepared in comparative example 1 of the present invention for nucleotide rapid nutritional supplementation.
  • Figure 8 is a flow chart of nucleotide detection in food supplement products based on nucleotide rapid nutritional supplements
  • Fig. 9 is the chromatogram 1 of standard nucleotide solution
  • Fig. 10 is the chromatogram 2 of standard nucleotide solution
  • Fig. 11 is the chromatogram 3 of standard nucleotide solution
  • Fig. 12 is the chromatogram 4 of standard nucleotide solution
  • Fig. 13 is the chromatogram 5 of standard nucleotide solution
  • Fig. 14 is the standard curve Fig. 1 of different nucleotides
  • Fig. 15 is the standard curve Fig. 2 of different nucleotides
  • Fig. 16 is the standard curve Fig. 3 of different nucleotides
  • Figure 17 is the detection result 1 of unknown concentration nucleotides
  • Figure 18 is the detection result 2 of nucleotides with unknown concentration.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients of raw materials include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include raw materials in parts by mass: curry chicken oil 45, sweet butter 8 grams, 3 grams of edible salt, 1 gram of red bowl monosodium glutamate, 8.5 grams of chicken powder, 50 grams of starch, 1 gram of edible alkali, 12 grams of dark soy sauce, 45 grams of oil consumption, 0.8 grams of I+G flavored nucleotide disodium, red 1 gram of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell.
  • the raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
  • Step (2) thawing the main ingredients: place the abalone in a clean environment for thawing in a natural state or place the abalone in a room temperature, humid and clean environment for thawing;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: place the abalone cleaned in step (3) in hot steam or hot water, and cook at 85°C for 30 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 1 hour to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 100 minutes, and keep warm at 45°C for 30 minutes after the end to obtain a thick broth;
  • Step (9) homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 65°C and 10MPa for 25 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 45°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: quick-freeze the filled and packaged semi-finished product for 1.5 hours with a quick-freezing machine, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 126°C for 12 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance:
  • Raw material requirements supplier qualification report, quarantine (COA), inspection qualified;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 1 hour to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and then keep warm at 45°C for 35 minutes to obtain a thick broth;
  • Step (9) homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2 hours, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include raw materials in parts by mass: 53 grams of curry chicken oil, sweet butter 12 grams, 4 grams of edible salt, 2 grams of red bowl monosodium glutamate, 9.5 grams of chicken powder, 58 grams of starch, 2 grams of edible alkali, 15 grams of dark soy sauce, 52 grams of oil consumption, 1.2 grams of I+G flavored nucleotide disodium, red 2 grams of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell.
  • the raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 95°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm and 100°C for 2 hours to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 120 minutes, and keep warm at 50°C for 40 minutes after the end to obtain a thick broth;
  • Step (9) homogenization: Transfer the thick soup prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 14MPa for 28 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 55°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2.5 hours, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 20 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance: take an appropriate amount of raw materials and place them in a clean white plate, observe the color and state under natural light, and smell their smell.
  • the raw materials are required to have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 100°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 2.5 hours to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue to heat for 130 minutes, and then keep warm at 50°C for 45 minutes to obtain a thick broth;
  • Step (9) homogenization: transfer the thick soup prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 80°C and 18MPa for 30 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool the homogenized thick broth to 25°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 55°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 3 hours, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 131°C for 32 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include the raw materials in parts by mass: 53 grams of curry chicken oil, sweet butter 12 grams, 4 grams of edible salt, 2 grams of red bowl monosodium glutamate, 9.5 grams of chicken powder, 58 grams of starch, 2 grams of edible alkali, 15 grams of dark soy sauce, 52 grams of oil consumption, 1.2 grams of I+G flavored nucleotide disodium, red 2 grams of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell.
  • the raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 20 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm and 100°C for 3 hours to obtain abalone extract;
  • Step (6) pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 150 minutes, and keep warm at 55°C for 50 minutes after the end to obtain a thick broth;
  • Step (9) homogenization: transfer the thick soup prepared in step (8) into a homogenizer through a pipeline and emulsify and homogenize at 85°C and 20MPa for 35 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool down the homogenized thick broth to 30°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 60°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 3 hours, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 131°C for 32 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • a food tonic product for rapid nutritional supplementation of nucleotides and its preparation method The food supplement product for rapid nutritional supplementation of nucleotides, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of broth; the abalone broth The broth is made from abalone, chicken skeleton, beef bone, fish bone and pork bone; the auxiliary materials include the following raw materials in parts by mass: curry chicken oil 50 grams, sweet butter 10 grams, edible salt 3.125 grams, red bowl 1.2 grams of monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of fuel consumption, 1 gram of disodium I+G nucleotide nucleotides, 1.5 grams of red yeast rice water.
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of broth; the abalone broth The broth is made from abalone, chicken skeleton, beef bone, fish bone and pork bone; the auxiliary materials include the
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance:
  • Raw material requirements supplier qualification report, quarantine (COA), inspection qualified;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: Add abalone meat, chicken skeleton, beef bone, fish bone, and pork bone to cold water, heat and boil, and boil for 1 hour at 1 atm and 100°C to obtain the extract;
  • Step (6) pressing: filter the extract to obtain the original soup and solid residue, use the soup dregs pressing system to squeeze out the remaining soup in the solid residue, let it stand for precipitation and filter, and separate to obtain the pressed soup; the original soup and the pressed The broth is mixed, precipitated, filtered, and separated to obtain broth;
  • Step (7) pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
  • Step (8) blending: transfer the broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and then keep warm at 45°C for 35 minutes to obtain a thick broth;
  • Step (9) homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
  • Step (10) heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
  • Step (11) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (12) quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2 hours, and then refrigerate at low temperature for later use;
  • Step (13) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
  • Step (14) metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  • step (14) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • the food tonic product of nucleotide rapid nutritional supplement including main ingredients and auxiliary materials
  • the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth
  • the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
  • the preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
  • Step (1) raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell.
  • the raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
  • Step (2) thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
  • Step (3) cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
  • Step (4) blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 20 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
  • Step (5) soup extraction: adding abalone meat to water, heating and boiling, and boiling at 1.5atm and 110°C for 1 hour to obtain abalone broth;
  • Step (6) pretreatment of auxiliary materials: Weigh the auxiliary materials according to the above-mentioned parts by mass, mix well and set aside;
  • Step (7) blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and keep warm at 45°C for 35 minutes after the end to obtain a thick broth;
  • Step (8) homogenization: transfer the thick broth prepared in step (8) to a homogenizer through pipelines and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
  • Step (9) heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
  • Step (10) filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
  • Step (11) quick-freezing and refrigerating: quick-freezing the filled and packaged semi-finished product for 2 hours with a quick-freezing machine, and then refrigerating at a low temperature for later use;
  • Step (12) high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
  • Step (13) metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector, after the detection is qualified, the packaging is completed, and after the packaging inspection is qualified, a food supplement product for rapid nutritional supplementation of nucleotides is prepared.
  • step (13) the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: ⁇ 1.5mm, SUS: ⁇ 2.0mm.
  • chromatographic column ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; injection volume: 10 ⁇ l; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol in a volume ratio of 95:5; isocratic elution.
  • flow rate 0.5ml/min
  • injection volume 10 ⁇ l
  • mobile phase 0.05 mol/L potassium dihydrogen phosphate and methanol in a volume ratio of 95:5
  • isocratic elution Input the standard solution into the high performance liquid chromatograph in turn.
  • the method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements comprises the following steps:
  • Step 1 Take samples 1 and 2 of food supplement products frozen at -60°C for rapid nutritional supplementation based on nucleotides, thaw at room temperature, take an appropriate amount of samples in a 10mLEP tube, put them in a high-speed centrifuge and centrifuge at 10,000r/min for 10min, pass After the 0.22um microporous filter membrane, put it into a 1.5mL auto-sampler bottle, store it in a refrigerator at 4°C, and take it out before analysis;
  • Step 4 supply sample 1 and sample 2 to high performance liquid chromatography respectively, and control the working parameters of high performance liquid chromatography as follows: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C ; Flow rate: 0.5ml/min; Injection volume: 10 ⁇ l; Mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; Isocratic elution; The concentration of nucleotides is obtained, as shown in Figure 17 and Figure 18.
  • the concentration of each substance in the food supplement product sample 1 is: cytosine nucleotide 4.3244ug/mL, adenine nucleotide 1.8689ug/mL, uracil nucleotide 7.7716ug/mL, guanosine nucleotide 32.5026 acid ug/mL and inosine nucleotide 53.4535ug/mL; the concentration of each substance in food supplement product sample 1 is: cytosine nucleotide 0, adenine nucleotide 0, uracil nucleotide 0, guanine nucleotide Purine nucleotide 74.2496ug/mL and hypoxanthine nucleotide 57.2852ug/mL.

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Abstract

A dietetic invigoration product capable of rapidly supplementing nutrients by nucleotide and a preparation method therefor, and a detection method for the nucleotide in the dietetic invigoration product. The dietetic invigoration product comprises main materials and auxiliary materials; abalones are taken as the main materials, the auxiliary materials are reasonably screened, and the materials are synergistically matched in a particular formula proportion, so that the prepared dietetic invigoration product is balanced in taste, and rich in nutritional ingredients such as amino acid, nucleotide, and protein. The preparation method for the dietetic invigoration product comprises: raw material acceptance-main material unfreezing-cleaning-blanching-soup extraction-squeezing-filtering-precipitation-auxiliary material pretreatment-blending-homogenizing-heat exchange-filling-labeling-quick-freezing refrigeration-high-temperature sterilization-metal detection-packaging inspection. The preparation method is simple and suitable for industrial production. In the detection method, an external standard method is utilized to correct high performance liquid chromatography, the dietetic invigoration product to be detected can be directly analyzed by means of the high performance liquid chromatography, a sample is not required to be treated, the operation is simple, the usage amount of the sample is small, the sensitivity is high, and the specificity is strong.

Description

一种核苷酸快速营养补充的食补产品和其制备方法及该食补产品中核苷酸的检测方法A food supplement product for rapid nutrient supplementation with nucleotides, a preparation method thereof, and a detection method for nucleotides in the food supplement product 技术领域technical field
本发明涉及食品加工技术领域和营养成分检测技术领域，具体涉及一种核苷酸快速营养补充的食补产品和其制备方法以及对该食补产品中核苷酸的检测方法。The invention relates to the technical field of food processing and the technical field of nutrient component detection, and in particular to a food supplement product for rapid nutritional supplementation with nucleotides, a preparation method thereof and a detection method for nucleotides in the food supplement product.
本申请基于2021年7月1日提出的中国发明申请第202110746475.5号和2021年7月20日提出的中国发明申请第202110819983.1号主张两项优先权，在此引用其内容。This application claims two priority rights based on Chinese Invention Application No. 202110746475.5 filed on July 1, 2021 and Chinese Invention Application No. 202110819983.1 filed on July 20, 2021, the contents of which are quoted here.
背景技术Background technique
免疫力是人体自身的防御机制，是人体识别和消灭外来侵入的任何异物，处理衰老、损伤、死亡、变性的自身细胞，以及识别和处理体内突变细胞和病毒感染细胞的能力，是人体识别和排除"异己"的生理反应。随着生活节奏加快，生活压力大、无规律饮食、服用药物不当、生活不规律等原因，造成部分人群免疫力下降，严重影响人体健康。在人体免疫功能低下时，身体呈现不抵抗状态，人体极易招致细菌、病毒等，易生病，特别是流行性感冒并发的感染，病情十分严重。核苷酸是生物体内重要的低分子化合物，具有许多生理功能，除作为DNA、RNA的前体，也作为生理、生化过程的调节物质参与体内物质代谢。核苷酸具有增强免疫能力、增强铁的吸收以助于大脑发育等作用。Immunity is the body's own defense mechanism. It is the body's ability to recognize and eliminate any foreign bodies invaded by the outside world, to deal with aging, damaged, dead, and denatured self-cells, and to recognize and deal with mutant cells and virus-infected cells in the body. Eliminate the physiological reactions of "other people". With the accelerated pace of life, high life pressure, irregular diet, improper medication, irregular life and other reasons, the immunity of some people has decreased, which seriously affects human health. When the immune function of the human body is low, the body presents a state of non-resistance, and the human body is very easy to cause bacteria, viruses, etc., and is easy to get sick, especially the concurrent infection of influenza, which is very serious. Nucleotides are important low-molecular compounds in living organisms and have many physiological functions. In addition to being the precursors of DNA and RNA, they are also involved in the metabolism of substances in the body as regulators of physiological and biochemical processes. Nucleotides can enhance immunity, enhance iron absorption to help brain development and so on.
鲍鱼是一种单壳软体动物，是中国传统的名贵食材，位居四大海洋之首，肉味鲜美，营养丰富，富含有氨基酸磷脂、糖脂、脂肪酸、甾醇、核酸类、色察等营养物质还含有Cu、Fe、Zn等元素，能够提高人体免疫力，抑制细菌、病毒生长。因此，以鲍鱼为主要原料，科学搭配配方，研究开发安全有效营养丰富的，具有增强免疫力的核苷酸快速营养补充的食补产品，对改善免疫力低下人群的健康状况具有重要意义。Abalone is a single-shell mollusc, which is a traditional Chinese precious food material. It ranks first among the four oceans. It is delicious in meat and rich in nutrition. It is rich in amino acid phospholipids, glycolipids, fatty acids, sterols, nucleic acids, and colorants Nutrients also contain Cu, Fe, Zn and other elements, which can improve human immunity and inhibit the growth of bacteria and viruses. Therefore, it is of great significance to improve the health status of immunocompromised people by using abalone as the main raw material, scientifically matching formula, researching and developing safe, effective and nutritious food supplement products with nucleotides for rapid nutritional supplementation that can enhance immunity.
核苷酸是一类由嘌呤碱或嘧啶碱、核糖或脱氧核糖以及磷酸三种物质组成的化合物，包括：CMP（胞嘧啶核苷酸）、UMP（尿嘧啶核苷酸）、AMP（腺嘌呤核苷酸）、GMP（鸟嘌呤核苷酸）和IMP（次黄嘌呤核苷酸）五种核苷酸。核苷酸类化合物具有重要的生物学功能，它们参与了生物体内几乎所有的生物化学反应。因此，对基于核苷酸快速营养补充的食补产品中核苷酸的检测显得尤为重要。但目前针对食补产品中五种核苷酸的检测的研究较少，因此有必要研发一种简单、高效且可靠的基于核苷酸快速营养补充的食补产品中核苷酸的检测方法，满足生产需求。Nucleotides are a class of compounds composed of purine or pyrimidine bases, ribose or deoxyribose, and phosphoric acid, including: CMP (cytosine nucleotide), UMP (uracil nucleotide), AMP (adenine Nucleotides), GMP (guanine nucleotides) and IMP (inosine nucleotides) five nucleotides. Nucleotide compounds have important biological functions, and they participate in almost all biochemical reactions in organisms. Therefore, the detection of nucleotides in food supplement products based on nucleotide rapid nutritional supplements is particularly important. However, there are few studies on the detection of the five nucleotides in food supplement products at present, so it is necessary to develop a simple, efficient and reliable method for the detection of nucleotides in food supplement products based on nucleotide rapid nutritional supplements to meet production needs .
技术解决方案technical solution
有鉴于此，本发明提供一种核苷酸快速营养补充的食补产品及其制备方法，以解决上述技术问题。In view of this, the present invention provides a food tonic product for rapid nutritional supplementation of nucleotides and a preparation method thereof to solve the above-mentioned technical problems.
本发明提供如下技术方案：The present invention provides following technical scheme:
一种核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000份；所述辅料包括以下质量份的原料：咖喱鸡油45份~53份、甜奶油8份~12份、食用盐3份~4份、红碗味精1份~2份、鸡粉8.5份~9.5份、淀粉50份~58份、食用碱1份~2份、老抽12份~15份、耗油45份~52份、I＋G呈味核苷酸二钠0.8份~1.2份、红曲米水1份~2份。A food tonic product for rapid nutritional supplementation of nucleotides, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 parts of abalone broth; the auxiliary materials include the following raw materials in parts by mass: curry chicken oil 45~ 53 parts, 8~12 parts of sweet butter, 3~4 parts of edible salt, 1~2 parts of red bowl monosodium glutamate, 8.5~9.5 parts of chicken powder, 50~58 parts of starch, 1~2 parts of edible alkali , 12~15 parts of dark soy sauce, 45~52 parts of oil consumption, 0.8~1.2 parts of disodium I+G flavored nucleotide, 1~2 parts of red yeast rice water.
优选地，所述主料包括以下质量份的原料：鲍鱼高汤1000份；所述辅料包括以下质量份的原料：咖喱鸡油50份、甜奶油10份、食用盐3.125份、红碗味精1.2份、鸡粉9.3份、淀粉55份、食用碱1.25份、老抽14份、耗油50份、I＋G呈味核苷酸二钠1份、红曲米水1.5份。Preferably, the main ingredients include the following raw materials by mass: 1000 parts of abalone stock; the auxiliary materials include the following raw materials by mass: 50 parts of chicken curry oil, 10 parts of sweet butter, 3.125 parts of edible salt, and 1.2 parts of red bowl monosodium glutamate , 9.3 parts of chicken powder, 55 parts of starch, 1.25 parts of edible alkali, 14 parts of dark soy sauce, 50 parts of fuel consumption, 1 part of disodium I+G flavored nucleotide, and 1.5 parts of red yeast rice water.
本发明另一目的在于提供一种上述核苷酸快速营养补充的食补产品的制备方法，依次包括以下步骤：原料验收—主料解冻—清洗—漂烫—汤汁提取—压榨—过滤—沉淀—辅料预处理—调配—均质—换热—灌装—标签—速冻冷藏—高温杀菌—金属检测—包装检查，全程无菌化生产。Another object of the present invention is to provide a method for preparing food tonic products for rapid nutritional supplementation of nucleotides, which includes the following steps in sequence: raw material acceptance—thawing of main ingredients—cleaning—blanching—soup extraction—pressing—filtration—precipitation— Excipient pretreatment - blending - homogenization - heat exchange - filling - labeling - quick freezing and refrigeration - high temperature sterilization - metal detection - packaging inspection, the whole process is aseptic production.
优选地，所述原料验收方法为：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物。Preferably, the raw material acceptance method is as follows: take an appropriate amount of raw materials and place them in a clean white plate, observe the color and state under natural light, and smell the smell. It is required that the raw materials have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision.
优选地，所述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：Preferably, the preparation method of the food tonic product of said nucleotide rapid nutritional supplement specifically comprises the following steps:
步骤（1），原料验收；Step (1), raw material acceptance;
步骤（2），主料解冻：将验收合格的鲍鱼置于洁净环境中自然状态放置解冻；Step (2), thawing of main ingredients: place the qualified abalone in a clean environment to thaw in a natural state;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将清洗干净后的鲍鱼放置于热蒸汽或热水中，在温度为85℃~100℃的条件下蒸煮30分钟~60分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: place the cleaned abalone in hot steam or hot water, and cook for 30 to 60 minutes at a temperature of 85°C to 100°C to remove foreign matter such as blood residue; Take out the abalone, remove the abalone meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中，然后加热熬煮，得到鲍鱼提取物；Step (5), soup extraction: add abalone meat to cold water, then heat and boil to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热100分钟~150分钟，结束后在45℃~55℃的温度下保温30分钟~50分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into the blending tank, add evenly mixed auxiliary materials, boil and continue heating for 100 minutes to 150 minutes, and keep warm at 45°C~55°C for 30 minutes to 50 minutes after the end, get thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中进行乳化均质，使其乳化细腻，分散均匀；Step (9), homogenization: transfer the thick soup prepared in step (8) into a homogenizer through pipelines for emulsification and homogenization, so that it can be emulsified finely and dispersed evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃~30℃；Step (10), heat exchange: cool the homogenized thick broth to 20°C~30°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至45℃~60℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 45°C~60°C, and then pump out the air The pressure tank is sealed and labeled to obtain a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻1.5小时~3小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: quick-freezing the filled and packaged semi-finished product for 1.5 hours to 3 hours with a quick-freezing machine, and then refrigerating at a low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品高温杀菌处理；Step (13), high-temperature sterilization: high-temperature sterilization of the quick-frozen and refrigerated semi-finished products;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
优选地，步骤（5）中，所述加热熬煮条件为1atm~2atm，100℃~120℃的条件下熬煮1小时~3小时。Preferably, in step (5), the heating and boiling conditions are 1 atm to 2 atm and 100° C. to 120° C. for 1 hour to 3 hours.
优选地，步骤（9）中，所述乳化均质的条件为：温度为65℃~85℃，压力为10MPa~20MPa，时间为25分钟~35分钟。Preferably, in step (9), the emulsification and homogenization conditions are as follows: the temperature is 65° C. to 85° C., the pressure is 10 MPa to 20 MPa, and the time is 25 minutes to 35 minutes.
优选地，步骤（13）中，所述高温杀菌处理的条件为：杀菌温度为126℃~131℃，时间为12分钟~32分钟。Preferably, in step (13), the conditions of the high-temperature sterilization treatment are: the sterilization temperature is 126° C. to 131° C., and the time is 12 minutes to 32 minutes.
优选地，步骤（14）中，所述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。Preferably, in step (14), the key limits of the detection accuracy of the metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
根据本发明的又一发明目的，本发明提供基于核苷酸快速营养补充的食补产品中核苷酸的检测方法。According to another object of the present invention, the present invention provides a method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements.
本发明解决上述问题所采用的技术方案是：基于核苷酸快速营养补充的食补产品中核苷酸的检测方法，所述核苷酸为胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸五种核苷酸；所述检测方法包括以下步骤：The technical solution adopted by the present invention to solve the above problems is: a method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements, the nucleotides are cytosine nucleotides, adenine nucleotides, uracil nucleotides Nucleotide, guanine nucleotide and inosine nucleotide five kinds of nucleotides; The detection method comprises the following steps:
步骤一：分别向高效液相色谱内供应含有核苷酸为胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸五种核苷酸的标准溶液，再以单个核苷酸的浓度比为X轴，单个核苷酸峰面积为Y轴，分别建立不同核苷酸的标准曲线；Step 1: Supply five kinds of nuclei containing nucleotides as cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine nucleotide to the high performance liquid chromatography respectively. The standard solution of nucleotides, and then the concentration ratio of a single nucleotide as the X-axis, and the peak area of a single nucleotide as the Y-axis, respectively establish standard curves for different nucleotides;
步骤二，取冷冻在-60℃基于核苷酸快速营养补充的食补产品样品，常温解冻，取适量的样品于10mLEP管中，放入高速离心机中10000r/min离心10min，过0.22um微孔滤膜后装入1.5mL自动进样瓶内，放入4℃冰箱保存，在分析前取出；Step 2: Take a food supplement product sample frozen at -60°C based on nucleotide rapid nutritional supplementation, thaw at room temperature, take an appropriate amount of sample in a 10mLEP tube, put it in a high-speed centrifuge at 10,000r/min for 10min, and pass through a 0.22um micropore After filtering the membrane, put it into a 1.5mL autosampler bottle, store it in a refrigerator at 4°C, and take it out before analysis;
步骤三，将样品供应到高效液相色谱，得到核苷酸的浓度。In step three, the sample is supplied to high performance liquid chromatography to obtain the concentration of nucleotides.
进一步地，步骤一中，所述高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱。Further, in step 1, the working parameters of the high performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; Sample volume: 10 μl; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
进一步地，步骤二中，所述高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱。Further, in step 2, the working parameters of the high performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; Sample volume: 10 μl; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
有益效果Beneficial effect
（1）本发明的核苷酸快速营养补充的食补产品，以鲍鱼为主料，合理筛选辅料原料，以特定的配方比例进协同配合，使得制备所得的食补产品味道均衡，香气浓郁，底味醇厚，富含氨基酸、核苷酸、蛋白质等营养成分，既可以满足消费者在口味方面的需求，又对人体免疫力提高有支持作用，满足消费者在营养、健康方面的需求。(1) The food tonic product for rapid nutritional supplementation of nucleotides of the present invention uses abalone as the main ingredient, rationally screens the raw materials of auxiliary materials, and cooperates with specific formula ratios, so that the prepared food tonic product has a balanced taste, strong aroma, and low flavor. It is full-bodied and rich in nutrients such as amino acids, nucleotides, and proteins. It can not only meet consumers' needs in terms of taste, but also support the improvement of human immunity and meet consumers' needs in terms of nutrition and health.
（2）本发明的核苷酸快速营养补充的食补产品以鲍鱼为原料，熬制浓高汤，通过控制温度、时间，最大程度上充分的保留了鲍鱼的营养成分和口味；通过采用特定比例的辅料原料，辅料原料间互相协调配合，有利于提高食补产品的风味，提高食补产品增强人体免疫力的效果。本发明在一定条件下对原料高汤进行熬煮，保证了主料中营养成分的充分提取和溶解，保证了产品的营养价值和风味；采用均质机均质保证了高汤体系中的分散物微粒化和均匀化，降低分散物尺度的同时提高了分布均匀性；采用汤渣压榨***将熬煮后的固体残留物通过压榨的方式挤出，充分利用原料，使产品中营养成分更完全、更丰富，浓度更高；本发明采用冷链速冻工艺，最大程度上保留了汤汁鲜美的同时，又获得了良好的保藏性，稳定性。(2) The food tonic product for rapid nutritional supplementation of nucleotides of the present invention uses abalone as a raw material, boils a thick broth, and by controlling the temperature and time, the nutritional components and taste of the abalone are fully retained to the greatest extent; by using a specific proportion of The raw materials of auxiliary materials and the coordination between raw materials of auxiliary materials are conducive to improving the flavor of food supplement products and improving the effect of food supplement products on enhancing human immunity. The invention boils the raw material broth under certain conditions, which ensures the full extraction and dissolution of the nutritional components in the main ingredients, and ensures the nutritional value and flavor of the product; the use of a homogenizer ensures the homogenization of the dispersed particles in the broth system Homogenization and homogenization, reducing the size of the dispersion and improving the uniformity of distribution; the soup dregs pressing system is used to squeeze out the solid residue after cooking, making full use of raw materials, so that the nutritional components in the product are more complete and more Rich, higher concentration; the invention adopts the cold chain quick-freezing process, which retains the deliciousness of the soup to the greatest extent, and at the same time obtains good preservation and stability.
（3）本发明制备方法简单，产品色香味俱全，适工业化生产。制得的核苷酸快速营养补充的食补产品营养丰富，无异腥味，核苷酸含量高，味道鲜美，使用方便，能够满足广大消费者的需求。(3) The preparation method of the present invention is simple, and the product is complete in color, fragrance and taste, and is suitable for industrial production. The prepared food tonic product for rapid nutritional supplementation of nucleotides is rich in nutrition, has no peculiar fishy smell, has high nucleotide content, is delicious in taste, is convenient to use, and can meet the needs of consumers.
（4）本发明采用外标法对高效液相色谱进行校正，可将待测食补产品直接使用高效液相色谱进行分析，样本无需处理，操作简单，样本用量少，灵敏度高，特异性强，可用于基于核苷酸快速营养补充的食补产品。(4) The present invention adopts the external standard method to calibrate the high performance liquid chromatography, and the food tonic product to be tested can be directly analyzed by high performance liquid chromatography, the sample does not need to be processed, the operation is simple, the sample consumption is small, the sensitivity is high, and the specificity is strong , which can be used in food supplement products based on nucleotide rapid nutritional supplements.
除了上面所描述的目的、特征和优点之外，本发明还有其它的目的、特征和优点。下面将对本发明作进一步详细的说明。In addition to the objects, features and advantages described above, the present invention has other objects, features and advantages. The present invention will be described in further detail below.
附图说明Description of drawings
构成本申请的一部分的附图用来提供对本发明的进一步理解，本发明的示意性实施例及其说明用于解释本发明，并不构成对本发明的不当限定。The accompanying drawings constituting a part of this application are used to provide further understanding of the present invention, and the schematic embodiments and descriptions of the present invention are used to explain the present invention, and do not constitute an improper limitation of the present invention.
图1为本发明核苷酸快速营养补充的食补产品的工艺流程图。Fig. 1 is the process flow chart of the food tonic product of nucleotide rapid nutritional supplement of the present invention.
图2为本发明实施例2制备的核苷酸快速营养补充的食补产品样品检测报告1。Fig. 2 is the test report 1 of the food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
图3为本发明实施例2制备的核苷酸快速营养补充的食补产品样品检测报告2。Fig. 3 is the detection report 2 of the food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
图4为本发明实施例2制备的核苷酸快速营养补充的食补产品样品检测报告3。Fig. 4 is a test report 3 of a food tonic product sample prepared in Example 2 of the present invention for rapid nutritional supplementation with nucleotides.
图5为本发明对比例1制备的核苷酸快速营养补充的食补产品样品检测报告1。Fig. 5 is a test report 1 of a food tonic product sample prepared in Comparative Example 1 of the present invention for rapid nutritional supplementation with nucleotides.
图6为本发明对比例1制备的核苷酸快速营养补充的食补产品样品检测报告2。Fig. 6 is the detection report 2 of the food tonic product sample prepared in comparative example 1 of the present invention for nucleotide rapid nutritional supplementation.
图7为本发明对比例1制备的核苷酸快速营养补充的食补产品样品检测报告3。Fig. 7 is the detection report 3 of the food tonic product sample prepared in comparative example 1 of the present invention for nucleotide rapid nutritional supplementation.
图8为基于核苷酸快速营养补充的食补产品中核苷酸检测的流程；Figure 8 is a flow chart of nucleotide detection in food supplement products based on nucleotide rapid nutritional supplements;
图9为标准核苷酸溶液的色谱图1；Fig. 9 is the chromatogram 1 of standard nucleotide solution;
图10为标准核苷酸溶液的色谱图2；Fig. 10 is the chromatogram 2 of standard nucleotide solution;
图11为标准核苷酸溶液的色谱图3；Fig. 11 is the chromatogram 3 of standard nucleotide solution;
图12为标准核苷酸溶液的色谱图4；Fig. 12 is the chromatogram 4 of standard nucleotide solution;
图13为标准核苷酸溶液的色谱图5；Fig. 13 is the chromatogram 5 of standard nucleotide solution;
图14为不同核苷酸的标准曲线图1；Fig. 14 is the standard curve Fig. 1 of different nucleotides;
图15为不同核苷酸的标准曲线图2；Fig. 15 is the standard curve Fig. 2 of different nucleotides;
图16为不同核苷酸的标准曲线图3；Fig. 16 is the standard curve Fig. 3 of different nucleotides;
图17为未知浓度核苷酸的检测结果1；Figure 17 is the detection result 1 of unknown concentration nucleotides;
图18为未知浓度核苷酸的检测结果2。Figure 18 is the detection result 2 of nucleotides with unknown concentration.
本发明的实施方式Embodiments of the present invention
为使本发明的目的、技术方案和优点更加清楚明白，下面结合实施方式和附图，对本发明做进一步详细说明。在此，本发明的示意性实施方式及其说明用于解释本发明，但并不作为对本发明的限定。In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings. Here, the exemplary embodiments and descriptions of the present invention are used to explain the present invention, but not to limit the present invention.
实施例1Example 1
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，原料所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油45、甜奶油8克、食用盐3克、红碗味精1克、鸡粉8.5克、淀粉50克、食用碱1克、老抽12克、耗油45克、I＋G呈味核苷酸二钠0.8克、红曲米水1克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients of raw materials include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include raw materials in parts by mass: curry chicken oil 45, sweet butter 8 grams, 3 grams of edible salt, 1 gram of red bowl monosodium glutamate, 8.5 grams of chicken powder, 50 grams of starch, 1 gram of edible alkali, 12 grams of dark soy sauce, 45 grams of oil consumption, 0.8 grams of I+G flavored nucleotide disodium, red 1 gram of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物；Step (1), raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell. The raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
步骤（2），主料解冻：将鲍鱼置于洁净环境中自然状态放置解冻或者将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a clean environment for thawing in a natural state or place the abalone in a room temperature, humid and clean environment for thawing;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在85℃下蒸煮30分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: place the abalone cleaned in step (3) in hot steam or hot water, and cook at 85°C for 30 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮1小时，得到鲍鱼提取物；Step (5), soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 1 hour to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热100分钟，结束后在45℃的温度下保温30分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 100 minutes, and keep warm at 45°C for 30 minutes after the end to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在65℃，10MPa下乳化均质25分钟，使其乳化细腻，分散均匀；Step (9), homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 65°C and 10MPa for 25 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃；Step (10), heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至45℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 45°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻1.5小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: quick-freeze the filled and packaged semi-finished product for 1.5 hours with a quick-freezing machine, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在126℃下高温杀菌处理12分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 126°C for 12 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
实施例2Example 2
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油50克、甜奶油10克、食用盐3.125克、红碗味精1.2克、鸡粉9.3克、淀粉55克、食用碱1.25克、老抽14克、耗油50克、I＋G呈味核苷酸二钠1克、红曲米水1.5克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：Step (1), raw material acceptance:
原料要求：供应商资质报告、检疫（COA）、检验合格；Raw material requirements: supplier qualification report, quarantine (COA), inspection qualified;
感官要求：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物。Sensory requirements: Take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell the smell. It is required that the raw materials have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision.
取适量试样置于洁净的白色盘(瓷盘或同类容器)中，在自然光下观察色泽和状态，闻其气味，色泽具有产品应有的色泽，气味具有产品应有的气味，无异味状态具有产品应有的状态，无正常视力可见外来异物；Take an appropriate amount of sample and place it in a clean white plate (porcelain plate or similar container), observe the color and state under natural light, and smell its smell. The color and luster have the color and luster that the product should have, the smell has the smell that the product should have, and there is no peculiar smell It has the proper state of the product, and there is no visible foreign matter with normal vision;
理化要求：依据《食品安全国家标准食品中挥发性盐基氮的测定》GB5009.228要求，畜禽挥发性盐基氮≤15(mg/100g)；污染物限量：畜禽内脏的污染物限量应符合GB2762中畜禽内脏的规定，除畜禽内脏以外的产品的污染物限量应符合GB2762中畜禽肉的规定。畜禽农药残留限量和兽药残留限量：农药残留量应符合GB2763的规定，兽药残留量应符合国家有关规定和公告；Physical and chemical requirements: According to the requirements of GB5009.228 of "Determination of Volatile Basic Nitrogen in Food in National Food Safety Standards", the volatile basic nitrogen of livestock and poultry is ≤15 (mg/100g); pollutant limit: the pollutant limit of livestock and poultry viscera It should comply with the regulations of livestock and poultry offal in GB2762, and the pollutant limit of products other than livestock and poultry offal should comply with the regulations of livestock and poultry meat in GB2762. Pesticide residue limits and veterinary drug residue limits for livestock and poultry: pesticide residues should comply with the provisions of GB2763, and veterinary drug residues should comply with relevant national regulations and announcements;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在90℃下蒸煮40分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮1小时，得到鲍鱼提取物；Step (5), soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 1 hour to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热110分钟，结束后在45℃的温度下保温35分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and then keep warm at 45°C for 35 minutes to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在70℃，12MPa下乳化均质30分钟，使其乳化细腻，分散均匀；Step (9), homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃；Step (10), heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至50℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻2小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2 hours, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在130℃下高温杀菌处理15分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
实施例3Example 3
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油53克、甜奶油12克、食用盐4克、红碗味精2克、鸡粉9.5克、淀粉58克、食用碱2克、老抽15克、耗油52克、I＋G呈味核苷酸二钠1.2克、红曲米水2克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include raw materials in parts by mass: 53 grams of curry chicken oil, sweet butter 12 grams, 4 grams of edible salt, 2 grams of red bowl monosodium glutamate, 9.5 grams of chicken powder, 58 grams of starch, 2 grams of edible alkali, 15 grams of dark soy sauce, 52 grams of oil consumption, 1.2 grams of I+G flavored nucleotide disodium, red 2 grams of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物；Step (1), raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell. The raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在95℃下蒸煮40分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 95°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮2小时，得到鲍鱼提取物；Step (5), soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm and 100°C for 2 hours to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热120分钟，结束后在50℃的温度下保温40分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 120 minutes, and keep warm at 50°C for 40 minutes after the end to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在70℃，14MPa下乳化均质28分钟，使其乳化细腻，分散均匀；Step (9), homogenization: Transfer the thick soup prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 14MPa for 28 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃；Step (10), heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至55℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 55°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻2.5小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2.5 hours, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在130℃下高温杀菌处理20分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 20 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
实施例4Example 4
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油50克、甜奶油10克、食用盐3.125克、红碗味精1.2克、鸡粉9.3克、淀粉55克、食用碱1.25克、老抽14克、耗油50克、I＋G呈味核苷酸二钠1克、红曲米水1.5克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物；Step (1), raw material acceptance: take an appropriate amount of raw materials and place them in a clean white plate, observe the color and state under natural light, and smell their smell. The raw materials are required to have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在100℃下蒸煮40分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 100°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮2.5小时，得到鲍鱼提取物；Step (5), soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm, 100°C for 2.5 hours to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热130分钟，结束后在50℃的温度下保温45分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue to heat for 130 minutes, and then keep warm at 50°C for 45 minutes to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在80℃，18MPa下乳化均质30分钟，使其乳化细腻，分散均匀；Step (9), homogenization: transfer the thick soup prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 80°C and 18MPa for 30 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至25℃；Step (10), heat exchange: cool the homogenized thick broth to 25°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至55℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 55°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻3小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 3 hours, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在131℃下高温杀菌处理32分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 131°C for 32 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
实施例5Example 5
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油53克、甜奶油12克、食用盐4克、红碗味精2克、鸡粉9.5克、淀粉58克、食用碱2克、老抽15克、耗油52克、I＋G呈味核苷酸二钠1.2克、红曲米水2克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include the raw materials in parts by mass: 53 grams of curry chicken oil, sweet butter 12 grams, 4 grams of edible salt, 2 grams of red bowl monosodium glutamate, 9.5 grams of chicken powder, 58 grams of starch, 2 grams of edible alkali, 15 grams of dark soy sauce, 52 grams of oil consumption, 1.2 grams of I+G flavored nucleotide disodium, red 2 grams of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物；Step (1), raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell. The raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在90℃下蒸煮20分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 20 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮3小时，得到鲍鱼提取物；Step (5), soup extraction: adding abalone meat to cold water, heating and boiling, and boiling at 1 atm and 100°C for 3 hours to obtain abalone extract;
步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热150分钟，结束后在55℃的温度下保温50分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 150 minutes, and keep warm at 55°C for 50 minutes after the end to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在85℃，20MPa下乳化均质35分钟，使其乳化细腻，分散均匀；Step (9), homogenization: transfer the thick soup prepared in step (8) into a homogenizer through a pipeline and emulsify and homogenize at 85°C and 20MPa for 35 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至30℃；Step (10), heat exchange: cool down the homogenized thick broth to 30°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至60℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 60°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻3小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 3 hours, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在131℃下高温杀菌处理32分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 131°C for 32 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
实施例6Example 6
一种核苷酸快速营养补充的食补产品及其制备方法核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：高汤1000克；所述鲍鱼高汤是以鲍鱼、鸡骨架、牛骨、鱼骨、猪骨为原料制得的高汤；所述辅料包括以下质量份的原料：咖喱鸡油50克、甜奶油10克、食用盐3.125克、红碗味精1.2克、鸡粉9.3克、淀粉55克、食用碱1.25克、老抽14克、耗油50克、I＋G呈味核苷酸二钠1克、红曲米水1.5克。A food tonic product for rapid nutritional supplementation of nucleotides and its preparation method The food supplement product for rapid nutritional supplementation of nucleotides, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of broth; the abalone broth The broth is made from abalone, chicken skeleton, beef bone, fish bone and pork bone; the auxiliary materials include the following raw materials in parts by mass: curry chicken oil 50 grams, sweet butter 10 grams, edible salt 3.125 grams, red bowl 1.2 grams of monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of fuel consumption, 1 gram of disodium I+G nucleotide nucleotides, 1.5 grams of red yeast rice water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：Step (1), raw material acceptance:
原料要求：供应商资质报告、检疫（COA）、检验合格；Raw material requirements: supplier qualification report, quarantine (COA), inspection qualified;
感官要求：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物。Sensory requirements: Take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell the smell. It is required that the raw materials have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision.
取适量试样置于洁净的白色盘(瓷盘或同类容器)中，在自然光下观察色泽和状态，闻其气味，色泽具有产品应有的色泽，气味具有产品应有的气味，无异味状态具有产品应有的状态，无正常视力可见外来异物；Take an appropriate amount of sample and place it in a clean white plate (porcelain plate or similar container), observe the color and state under natural light, and smell its smell. The color and luster have the color and luster that the product should have, the smell has the smell that the product should have, and there is no peculiar smell It has the proper state of the product, and there is no visible foreign matter with normal vision;
理化要求：依据《食品安全国家标准食品中挥发性盐基氮的测定》GB5009.228要求，畜禽挥发性盐基氮≤15(mg/100g)；污染物限量：畜禽内脏的污染物限量应符合GB2762中畜禽内脏的规定，除畜禽内脏以外的产品的污染物限量应符合GB2762中畜禽肉的规定。畜禽农药残留限量和兽药残留限量：农药残留量应符合GB2763的规定，兽药残留量应符合国家有关规定和公告；Physical and chemical requirements: According to the requirements of GB5009.228 of "Determination of Volatile Basic Nitrogen in Food in National Food Safety Standards", the volatile basic nitrogen of livestock and poultry is ≤15 (mg/100g); pollutant limit: the pollutant limit of livestock and poultry viscera It should comply with the regulations of livestock and poultry offal in GB2762, and the pollutant limit of products other than livestock and poultry offal should comply with the regulations of livestock and poultry meat in GB2762. Pesticide residue limits and veterinary drug residue limits for livestock and poultry: pesticide residues should comply with the provisions of GB2763, and veterinary drug residues should comply with relevant national regulations and announcements;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在90℃下蒸煮40分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 40 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉、鸡骨架、牛骨、鱼骨、猪骨加入到冷水中加热熬煮，在1atm，100℃的条件下熬煮1小时，得到提取物；Step (5), soup extraction: Add abalone meat, chicken skeleton, beef bone, fish bone, and pork bone to cold water, heat and boil, and boil for 1 hour at 1 atm and 100°C to obtain the extract;
步骤（6），压榨：将提取物过滤得到原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将原汤与压榨汤汁混合，经沉淀，过滤，分离，得到高汤；Step (6), pressing: filter the extract to obtain the original soup and solid residue, use the soup dregs pressing system to squeeze out the remaining soup in the solid residue, let it stand for precipitation and filter, and separate to obtain the pressed soup; the original soup and the pressed The broth is mixed, precipitated, filtered, and separated to obtain broth;
步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
步骤（8），调配：将高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热110分钟，结束后在45℃的温度下保温35分钟，得到浓高汤；Step (8), blending: transfer the broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and then keep warm at 45°C for 35 minutes to obtain a thick broth;
步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中在70℃，12MPa下乳化均质30分钟，使其乳化细腻，分散均匀；Step (9), homogenization: Transfer the thick broth prepared in step (8) to a homogenizer through a pipeline and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃；Step (10), heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至50℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻2小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: use a quick-freezing machine to freeze the filled and packaged semi-finished product for 2 hours, and then refrigerate at low temperature for later use;
步骤（13），高温杀菌：将速冻冷藏的半成品在130℃下高温杀菌处理15分钟；Step (13), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
步骤（14）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (14), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
对比例1Comparative example 1
一种核苷酸快速营养补充的食补产品及其制备方法Nucleotide quick nutritional supplement food tonic product and preparation method thereof
核苷酸快速营养补充的食补产品，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000克；所述辅料包括以下质量份的原料：咖喱鸡油50克、甜奶油10克、食用盐3.125克、红碗味精1.2克、鸡粉9.3克、淀粉55克、食用碱1.25克、老抽14克、耗油50克、I＋G呈味核苷酸二钠1克、红曲米水1.5克。The food tonic product of nucleotide rapid nutritional supplement, including main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 grams of abalone broth; the auxiliary materials include raw materials in parts by mass: 50 grams of curry chicken oil, sweet butter 10 grams, 3.125 grams of edible salt, 1.2 grams of red bowl monosodium glutamate, 9.3 grams of chicken powder, 55 grams of starch, 1.25 grams of edible alkali, 14 grams of dark soy sauce, 50 grams of oil consumption, 1 gram of I+G flavored nucleotide disodium, red 1.5 grams of koji water.
上述核苷酸快速营养补充的食补产品的制备方法，具体包括以下步骤：The preparation method of the food tonic product of the above-mentioned nucleotide rapid nutritional supplement specifically includes the following steps:
步骤（1），原料验收：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物；Step (1), raw material acceptance: take an appropriate amount of raw materials and put them in a clean white plate, observe the color and state under natural light, and smell their smell. The raw materials are required to be normal in color, fresh and odorless, and no foreign matter can be seen with normal vision;
步骤（2），主料解冻：将鲍鱼置于常温湿润洁净环境中放置解冻；Step (2), thawing the main ingredients: place the abalone in a humid and clean environment at room temperature to thaw;
步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
步骤（4），漂烫：将步骤（3）清洗干净后的鲍鱼放置于热蒸汽或热水中，在90℃下蒸煮20分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: Place the abalone cleaned in step (3) in hot steam or hot water, and cook at 90°C for 20 minutes to remove foreign matter such as blood residue; take out the cooked abalone, and put the abalone Remove the meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
步骤（5），汤汁提取：将鲍鱼肉加入水中加热熬煮，在1.5atm，110℃的条件下熬煮1小时，得到鲍鱼高汤；Step (5), soup extraction: adding abalone meat to water, heating and boiling, and boiling at 1.5atm and 110°C for 1 hour to obtain abalone broth;
步骤（6），辅料预处理：按上述质量份称取辅料，充分混合均匀后备用；Step (6), pretreatment of auxiliary materials: Weigh the auxiliary materials according to the above-mentioned parts by mass, mix well and set aside;
步骤（7），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热110分钟，结束后在45℃的温度下保温35分钟，得到浓高汤；Step (7), blending: transfer the abalone broth into a blending tank, add evenly mixed auxiliary materials, boil and continue heating for 110 minutes, and keep warm at 45°C for 35 minutes after the end to obtain a thick broth;
步骤（8），均质：将步骤（8）制得的浓高汤经管道转入均质机中在70℃，12MPa下乳化均质30分钟，使其乳化细腻，分散均匀；Step (8), homogenization: transfer the thick broth prepared in step (8) to a homogenizer through pipelines and emulsify and homogenize at 70°C and 12MPa for 30 minutes to make it emulsify finely and disperse evenly;
步骤（9），换热：将均质后的浓高汤通过换热降温冷却至20℃；Step (9), heat exchange: cool down the homogenized thick broth to 20°C through heat exchange;
步骤（10），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至50℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (10), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 50°C, then pump out the air and press the tank to seal , affix a label to get a semi-finished product;
步骤（11），速冻冷藏：将灌装包装后的半成品利用速冻机速冻2小时，然后低温冷藏备用；Step (11), quick-freezing and refrigerating: quick-freezing the filled and packaged semi-finished product for 2 hours with a quick-freezing machine, and then refrigerating at a low temperature for later use;
步骤（12），高温杀菌：将速冻冷藏的半成品在130℃下高温杀菌处理15分钟；Step (12), high-temperature sterilization: sterilize the quick-frozen and refrigerated semi-finished product at 130°C for 15 minutes;
步骤（13），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (13), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector, after the detection is qualified, the packaging is completed, and after the packaging inspection is qualified, a food supplement product for rapid nutritional supplementation of nucleotides is prepared.
步骤（13）中，上述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。In step (13), the key limits of the detection accuracy of the above-mentioned metal detection are: Fe: Φ1.5mm, SUS: Φ2.0mm.
试验例Test case
为了确定本发明制备的核苷酸快速营养补充的食补产品的性能，现对实施例2、对比例1及实施例6制备的核苷酸快速营养补充的食补产品分别进行抽样样品检测。将抽取作为样品的核苷酸快速营养补充的食补产品按照GB5413.40-2016的标准进行检测，检测结果如表1所示。In order to determine the performance of the food tonic products for rapid nutritional supplementation of nucleotides prepared in the present invention, the food supplement products for rapid nutritional supplementation of nucleotides prepared in Example 2, Comparative Example 1 and Example 6 are now sampled for testing. The food tonic products of nucleotide rapid nutritional supplement extracted as samples were tested according to the standard of GB5413.40-2016, and the test results are shown in Table 1.
表1 Table 1
Figure 613399dest_path_image001
Figure 613399dest_path_image001
实施例7Example 7
基于核苷酸快速营养补充的食补产品中核苷酸的检测方法Detection method of nucleotides in food supplement products based on nucleotide rapid nutritional supplement
1、建立标准曲线1. Establish a standard curve
控制高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱。依次向高效液相色谱内输入标准溶液。The working parameters of high-performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; injection volume: 10μl; mobile phase: 0.05 mol/L potassium dihydrogen phosphate and methanol in a volume ratio of 95:5; isocratic elution. Input the standard solution into the high performance liquid chromatograph in turn.
（1）配制胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的浓度均为10μg/mL的标准溶液，输入高效液相色谱中，得到五个保留时间和对应的峰面积，如表2和图9所示：(1) Prepare a standard solution with a concentration of 10 μg/mL of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosine nucleotide, and input it into the high performance liquid phase In the chromatogram, five retention times and corresponding peak areas are obtained, as shown in Table 2 and Figure 9:
表2 Table 2
Figure 290368dest_path_image002
Figure 290368dest_path_image002
（2）输入胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的浓度均为20μg/mL的标准溶液，得到五个保留时间和对应的峰面积，如表3和图10所示：(2) Input a standard solution with a concentration of 20 μg/mL of cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and inosine nucleotide to obtain five reserved Time and corresponding peak area, as shown in Table 3 and Figure 10:
表3table 3
Figure 790620dest_path_image003
Figure 790620dest_path_image003
（3）输入不同浓度的胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的标准溶液，得到五个保留时间和对应的峰面积，如表4和图11所示：(3) Input standard solutions of cytosine nucleotides, adenine nucleotides, uracil nucleotides, guanine nucleotides and inosine nucleotides at different concentrations to obtain five retention times and corresponding Peak area, as shown in Table 4 and Figure 11:
表4Table 4
Figure 804712dest_path_image004
Figure 804712dest_path_image004
（4）输入不同浓度的胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的标准溶液，得到五个保留时间和对应的峰面积，如表5和图12所示：(4) Input standard solutions of cytosine nucleotides, adenine nucleotides, uracil nucleotides, guanine nucleotides and inosine nucleotides at different concentrations to obtain five retention times and corresponding Peak area, as shown in Table 5 and Figure 12:
表5table 5
Figure 339599dest_path_image005
Figure 339599dest_path_image005
（5）输入不同浓度的胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的标准溶液，得到五个保留时间和对应的峰面积，如表6和图13所示：(5) Input standard solutions of cytosine nucleotides, adenine nucleotides, uracil nucleotides, guanine nucleotides and hypoxanthine nucleotides at different concentrations to obtain five retention times and corresponding Peak area, as shown in Table 6 and Figure 13:
表6Table 6
Figure 751251dest_path_image006
Figure 751251dest_path_image006
（6）通过外标法分别建立胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸的标准曲线，如图14~图16所示。(6) Establish the standard curves of cytosine nucleotides, adenine nucleotides, uracil nucleotides, guanine nucleotides and hypoxanthine nucleotides by external standard method, as shown in Figure 14~Figure 16 shown.
2、基于核苷酸快速营养补充的食补产品样品的检测2. Detection of food tonic product samples based on nucleotide rapid nutritional supplement
在标准曲线的基础上，所述基于核苷酸快速营养补充的食补产品中核苷酸检测方法包括以下步骤：On the basis of the standard curve, the method for detecting nucleotides in food supplement products based on nucleotide rapid nutritional supplements comprises the following steps:
步骤一：取冷冻在-60℃基于核苷酸快速营养补充的食补产品样品1和样品2，常温解冻，取适量的样品于10mLEP管中，放入高速离心机中10000r/min离心10min，过0.22um微孔滤膜后装入1.5mL自动进样瓶内，放入4℃冰箱保存，在分析前取出；Step 1: Take samples 1 and 2 of food supplement products frozen at -60°C for rapid nutritional supplementation based on nucleotides, thaw at room temperature, take an appropriate amount of samples in a 10mLEP tube, put them in a high-speed centrifuge and centrifuge at 10,000r/min for 10min, pass After the 0.22um microporous filter membrane, put it into a 1.5mL auto-sampler bottle, store it in a refrigerator at 4°C, and take it out before analysis;
步骤四，将样品1和样品2分别供应到高效液相色谱，控制高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱；得到核苷酸的浓度，如图17和图18所示。由图可知，食补产品样品1中各物质的浓度为：胞嘧啶核苷酸4.3244ug/mL、腺嘌呤核苷酸1.8689ug/mL、尿嘧啶核苷酸7.7716ug/mL、鸟嘌呤核苷32.5026酸ug/mL和次黄腺嘌呤核苷酸53.4535ug/mL；食补产品样品1中各物质的浓度为：胞嘧啶核苷酸0、腺嘌呤核苷酸0、尿嘧啶核苷酸0、鸟嘌呤核苷酸74.2496ug/mL和次黄腺嘌呤核苷酸57.2852ug/mL。Step 4, supply sample 1 and sample 2 to high performance liquid chromatography respectively, and control the working parameters of high performance liquid chromatography as follows: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength: 254nm; column temperature: 30°C ; Flow rate: 0.5ml/min; Injection volume: 10 μl; Mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; Isocratic elution; The concentration of nucleotides is obtained, as shown in Figure 17 and Figure 18. It can be seen from the figure that the concentration of each substance in the food supplement product sample 1 is: cytosine nucleotide 4.3244ug/mL, adenine nucleotide 1.8689ug/mL, uracil nucleotide 7.7716ug/mL, guanosine nucleotide 32.5026 acid ug/mL and inosine nucleotide 53.4535ug/mL; the concentration of each substance in food supplement product sample 1 is: cytosine nucleotide 0, adenine nucleotide 0, uracil nucleotide 0, guanine nucleotide Purine nucleotide 74.2496ug/mL and hypoxanthine nucleotide 57.2852ug/mL.
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，对于本领域的技术人员来说，本发明实施例可以有各种更改和变化。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, various modifications and changes may be made to the embodiments of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (12)

  1. 一种核苷酸快速营养补充的食补产品，其特征在于，包括主料和辅料，所述主料包括以下质量份的原料：鲍鱼高汤1000份；所述辅料包括以下质量份的原料：咖喱鸡油45份~53份、甜奶油8份~12份、食用盐3份~4份、红碗味精1份~2份、鸡粉8.5份~9.5份、淀粉50份~58份、食用碱1份~2份、老抽12份~15份、耗油45份~52份、I＋G呈味核苷酸二钠0.8份~1.2份、红曲米水1份~2份。A food tonic product for rapid nutritional supplementation of nucleotides, characterized in that it includes main ingredients and auxiliary materials, the main ingredients include the following raw materials in parts by mass: 1000 parts of abalone broth; the auxiliary materials include raw materials in parts by mass: 45~53 parts of oil, 8~12 parts of sweet butter, 3~4 parts of edible salt, 1~2 parts of red bowl monosodium glutamate, 8.5~9.5 parts of chicken powder, 50~58 parts of starch, 1 part of edible alkali 1-2 servings, dark soy sauce 12-15 servings, fuel consumption 45-52 servings, I+G flavored nucleotide disodium 0.8-1.2 servings, red yeast rice water 1-2 servings.
  2. 根据权利要求1所述的核苷酸快速营养补充的食补产品，其特征在于，所述主料包括以下质量份的原料：鲍鱼高汤1000份；所述辅料包括以下质量份的原料：咖喱鸡油50份、甜奶油10份、食用盐3.125份、红碗味精1.2份、鸡粉9.3份、淀粉55份、食用碱1.25份、老抽14份、耗油50份、I＋G呈味核苷酸二钠1份、红曲米水1.5份。The food tonic product for rapid nutritional supplementation of nucleotides according to claim 1, wherein the main ingredient includes the following raw materials in parts by mass: 1000 parts of abalone broth; the auxiliary materials include raw materials in parts by mass: curry chicken oil 50 parts, 10 parts of sweet butter, 3.125 parts of edible salt, 1.2 parts of red bowl monosodium glutamate, 9.3 parts of chicken powder, 55 parts of starch, 1.25 parts of edible alkali, 14 parts of dark soy sauce, 50 parts of oil consumption, I+G taste nucleotide two Sodium 1 part, red yeast rice water 1.5 parts.
  3. 一种根据权利要求1~2任意一项所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，依次包括以下步骤：原料验收—主料解冻—清洗—漂烫—汤汁提取—压榨—过滤—沉淀—辅料预处理—调配—均质—换热—灌装—标签—速冻冷藏—高温杀菌—金属检测—包装检查，全程无菌化生产。A method for preparing food tonic products for rapid nutritional supplementation of nucleotides according to any one of claims 1 to 2, characterized in that it comprises the following steps in sequence: raw material acceptance - thawing of main ingredients - cleaning - blanching - soup Extraction—squeezing—filtration—precipitation—excipient pretreatment—deployment—homogenization—heat exchange—filling—labeling—quick freezing and refrigeration—high temperature sterilization—metal detection—packaging inspection, the whole process is aseptic production.
  4. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，所述原料验收方法为：取适量原料置于洁净的白色盘中，在自然光下观察色泽和状态，闻其气味，要求原料色泽正常，新鲜无异味，无正常视力可见外来异物。According to claim 3, the preparation method of food tonic products for rapid nutritional supplementation of nucleotides is characterized in that the raw material acceptance method is as follows: take an appropriate amount of raw materials and place them in a clean white plate, observe the color and state under natural light, To smell its smell, the raw materials are required to have normal color, freshness and no peculiar smell, and no foreign matter can be seen with normal vision.
  5. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，具体包括以下步骤：The preparation method of the food tonic product of nucleotide quick nutrition supplement according to claim 3, is characterized in that, specifically comprises the following steps:
    步骤（1），原料验收；Step (1), raw material acceptance;
    步骤（2），主料解冻：将验收合格的鲍鱼置于洁净环境中自然状态放置解冻；Step (2), thawing of main ingredients: place the qualified abalone in a clean environment to thaw in a natural state;
    步骤（3），清洗：低温状态下，用清水清洗鲍鱼，除去粘附在鲍鱼表面的大部分沙粒及异物；Step (3), cleaning: wash the abalone with clean water at low temperature to remove most of the sand and foreign matter adhering to the surface of the abalone;
    步骤（4），漂烫：将清洗干净后的鲍鱼放置于热蒸汽或热水中，在温度为85℃~100℃的条件下蒸煮30分钟~60分钟，除去血渣等异物；将煮好的鲍鱼取出，将鲍鱼肉从鲍鱼壳上取下，去除内脏及裙边，并用清水再次清洗鲍鱼肉，彻底除去沙粒及异物；Step (4), blanching: place the cleaned abalone in hot steam or hot water, and cook for 30 to 60 minutes at a temperature of 85°C to 100°C to remove foreign matter such as blood residue; Take out the abalone, remove the abalone meat from the abalone shell, remove the viscera and skirt, and wash the abalone meat again with clean water to completely remove sand and foreign matter;
    步骤（5），汤汁提取：将鲍鱼肉加入到冷水中，然后加热熬煮，得到鲍鱼提取物；Step (5), soup extraction: add abalone meat to cold water, then heat and boil to obtain abalone extract;
    步骤（6），压榨：将鲍鱼提取物过滤得到鲍鱼原汤和固体残留物，利用汤渣压榨***将固体残留物中残留的汤汁压榨出来，静置沉淀过滤后分离得到压榨汤汁；将鲍鱼原汤与压榨汤汁混合，经沉淀，过滤，分离，得到鲍鱼高汤；Step (6), pressing: filter the abalone extract to obtain the original abalone soup and solid residue, use the soup slag squeezing system to squeeze out the soup remaining in the solid residue, let it stand for sedimentation and filter, and separate to obtain the squeezed soup; the abalone The original soup is mixed with the squeezed soup, precipitated, filtered, and separated to obtain abalone broth;
    步骤（7），辅料预处理：按所述质量份称取辅料，充分混合均匀后备用；Step (7), pretreatment of excipients: Weigh the excipients according to the mass parts, mix well and set aside;
    步骤（8），调配：将鲍鱼高汤转入调配罐中，加入混合均匀的辅料，煮沸后持续加热100分钟~150分钟，结束后在45℃~55℃的温度下保温30分钟~50分钟，得到浓高汤；Step (8), blending: transfer the abalone broth into the blending tank, add evenly mixed auxiliary materials, boil and continue heating for 100 minutes to 150 minutes, and keep warm at 45°C~55°C for 30 minutes to 50 minutes after the end, get thick broth;
    步骤（9），均质：将步骤（8）制得的浓高汤经管道转入均质机中进行乳化均质，使其乳化细腻，分散均匀；Step (9), homogenization: transfer the thick soup prepared in step (8) into a homogenizer through pipelines for emulsification and homogenization, so that it can be emulsified finely and dispersed evenly;
    步骤（10），换热：将均质后的浓高汤通过换热降温冷却至20℃~30℃；Step (10), heat exchange: cool the homogenized thick broth to 20°C~30°C through heat exchange;
    步骤（11），灌装：将冷却后的浓高汤按规格灌装至无菌灌装容器中，并将无菌灌装容器中浓高汤的中心温度加热至45℃~60℃，然后抽出空气压罐密封，贴上标签，得到半成品；Step (11), filling: Fill the cooled thick broth into aseptic filling containers according to the specifications, and heat the center temperature of the thick broth in the aseptic filling container to 45°C~60°C, and then pump out the air The pressure tank is sealed and labeled to obtain a semi-finished product;
    步骤（12），速冻冷藏：将灌装包装后的半成品利用速冻机速冻1.5小时~3小时，然后低温冷藏备用；Step (12), quick-freezing and refrigerating: quick-freezing the filled and packaged semi-finished product for 1.5 hours to 3 hours with a quick-freezing machine, and then refrigerating at a low temperature for later use;
    步骤（13），高温杀菌：将速冻冷藏的半成品高温杀菌处理；Step (13), high-temperature sterilization: high-temperature sterilization of the quick-frozen and refrigerated semi-finished products;
    步骤（14），金属检测：将杀菌处理后的半成品通过金属探测器进行金属检测，检测合格后，完成包装，经包装检查合格后，制得核苷酸快速营养补充的食补产品。Step (14), metal detection: metal detection is carried out on the sterilized semi-finished product through a metal detector. After the detection is qualified, the packaging is completed. After the packaging inspection is qualified, the food supplement product of nucleotide rapid nutritional supplement is prepared.
  6. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，步骤（5）中，所述加热熬煮条件为1atm~2atm，100℃~120℃的条件下熬煮1小时~3小时。The preparation method of food tonic products for rapid nucleotide nutritional supplementation according to claim 3, characterized in that in step (5), the heating and boiling conditions are 1 atm~2 atm, 100°C~120°C Cook for 1 hour to 3 hours.
  7. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，步骤（9）中，所述乳化均质的条件为：温度为65℃~85℃，压力为10MPa~20MPa，时间为25分钟~35分钟。The preparation method of food tonic products for rapid nutritional supplementation of nucleotides according to claim 3, characterized in that, in step (9), the conditions for emulsification and homogeneity are: temperature is 65°C~85°C, pressure is 10MPa ~20MPa, the time is 25 minutes to 35 minutes.
  8. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，步骤（13）中，所述高温杀菌处理的条件为：杀菌温度为126℃~131℃，时间为12分钟~32分钟。According to claim 3, the preparation method of food tonic products for rapid nutritional supplementation of nucleotides is characterized in that, in step (13), the conditions of the high-temperature sterilization treatment are: the sterilization temperature is 126°C~131°C, and the time is 12 minutes to 32 minutes.
  9. 根据权利要求3所述的核苷酸快速营养补充的食补产品的制备方法，其特征在于，步骤（14）中，所述金属探测的探测精度关键限值为：Fe:Φ1.5mm，SUS:Φ2.0mm。The method for preparing food tonic products for rapid nucleotide nutritional supplementation according to claim 3, characterized in that, in step (14), the detection accuracy critical limit of the metal detection is: Fe: Φ1.5 mm, SUS: Φ2.0mm.
  10. 基于核苷酸快速营养补充的食补产品中核苷酸的检测方法，其特征在于，所述核苷酸为胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸五种核苷酸；所述检测方法包括以下步骤：The method for detecting nucleotides in food tonic products based on nucleotide rapid nutritional supplementation is characterized in that the nucleotides are cytosine nucleotides, adenine nucleotides, uracil nucleotides, and guanine nucleotides and five nucleotides of inosine nucleotide; the detection method comprises the following steps:
    步骤一：分别向高效液相色谱内供应含有核苷酸为胞嘧啶核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、鸟嘌呤核苷酸和次黄腺嘌呤核苷酸五种核苷酸的标准溶液，再以单个核苷酸的浓度比为X轴，单个核苷酸峰面积为Y轴，分别建立不同核苷酸的标准曲线；Step 1: Supply five kinds of nucleosides containing nucleotides as cytosine nucleotide, adenine nucleotide, uracil nucleotide, guanine nucleotide and hypoxanthine nucleotide to the high performance liquid chromatography respectively. The standard solution of nucleotides, and then the concentration ratio of a single nucleotide as the X-axis, and the peak area of a single nucleotide as the Y-axis, respectively establish standard curves for different nucleotides;
    步骤二，取冷冻在-60℃基于核苷酸快速营养补充的食补产品样品，常温解冻，取适量的样品于10mLEP管中，放入高速离心机中10000r/min离心10min，过0.22um微孔滤膜后装入1.5mL自动进样瓶内，放入4℃冰箱保存，在分析前取出；Step 2: Take a food supplement product sample frozen at -60°C for rapid nutritional supplementation based on nucleotides, thaw at room temperature, take an appropriate amount of sample in a 10mLEP tube, put it in a high-speed centrifuge at 10,000r/min for 10min, and pass through a 0.22um micropore After filtering the membrane, put it into a 1.5mL autosampler bottle, store it in a refrigerator at 4°C, and take it out before analysis;
    步骤三，将样品供应到高效液相色谱，得到核苷酸的浓度。In step three, the sample is supplied to high performance liquid chromatography to obtain the concentration of nucleotides.
  11.  根据权利要求10所述的检测方法，其特征在于，步骤一中，所述高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱。The detection method according to claim 10, wherein in step one, the working parameters of the high performance liquid chromatography are: chromatographic column: ZORBAX Eclipse Plus C18 (4.6*250mm5-Micron); detection wavelength is: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; injection volume: 10μl; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
  12. 根据权利要求10所述的检测方法，其特征在于，步骤二中，所述高效液相色谱的工作参数为：色谱柱：ZORBAXEclipsePlusC18(4.6*250mm5-Micron)；检测波长为：254nm；柱温：30℃；流速：0.5ml/min；进样量：10μl；流动相：体积比为95:5的0.05mol/L磷酸二氢钾和甲醇；等度洗脱。 The detection method according to claim 10, wherein, in step 2, the working parameters of the HPLC are: chromatographic column: ZORBAX EclipsePlusC18 (4.6*250mm5-Micron); detection wavelength is: 254nm; column temperature: 30°C; flow rate: 0.5ml/min; injection volume: 10μl; mobile phase: 0.05mol/L potassium dihydrogen phosphate and methanol with a volume ratio of 95:5; isocratic elution.
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