WO2023217767A3 - Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning - Google Patents

Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning Download PDF

Info

Publication number
WO2023217767A3
WO2023217767A3 PCT/EP2023/062247 EP2023062247W WO2023217767A3 WO 2023217767 A3 WO2023217767 A3 WO 2023217767A3 EP 2023062247 W EP2023062247 W EP 2023062247W WO 2023217767 A3 WO2023217767 A3 WO 2023217767A3
Authority
WO
WIPO (PCT)
Prior art keywords
organoid
farms
regional
patterning
aggregation
Prior art date
Application number
PCT/EP2023/062247
Other languages
French (fr)
Other versions
WO2023217767A2 (en
Inventor
Christopher Lawrence GRIGSBY
Kaja I. RITZAU-REID
Richard Wang
Ruoxiao XIE
Daniel REUMANN
Jürgen A. KNOBLICH
James P. Armstrong
Jonathon YEOW
Molly M. Stevens
Original Assignee
Imperial College Innovations Limited
Institut Für Molekulare Biotechnologie Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial College Innovations Limited, Institut Für Molekulare Biotechnologie Gmbh filed Critical Imperial College Innovations Limited
Publication of WO2023217767A2 publication Critical patent/WO2023217767A2/en
Publication of WO2023217767A3 publication Critical patent/WO2023217767A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y70/00—Materials specially adapted for additive manufacturing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y80/00—Products made by additive manufacturing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan

Abstract

Methods and products for preparing organoids in regular arrays on scaffolds are proposed.
PCT/EP2023/062247 2022-05-09 2023-05-09 Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning WO2023217767A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2206768.0 2022-05-09
GB202206768 2022-05-09

Publications (2)

Publication Number Publication Date
WO2023217767A2 WO2023217767A2 (en) 2023-11-16
WO2023217767A3 true WO2023217767A3 (en) 2024-02-15

Family

ID=86497451

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2023/062247 WO2023217767A2 (en) 2022-05-09 2023-05-09 Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning

Country Status (1)

Country Link
WO (1) WO2023217767A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011008986A2 (en) * 2009-07-15 2011-01-20 California Institute Of Technology A method applying hemodynamic forcing and klf2 to initiate the growth and development of cardiac valves
US20170246350A1 (en) * 2010-03-04 2017-08-31 University Of Pittsburgh - Of The Commonwealth System Of Higher Education "Bioengineered Human Corneal Stromal Tissue"
US20180243480A1 (en) * 2015-08-17 2018-08-30 The Johns Hopkins University Mesenchymal cell-binding composite material for tissue restoration
US20190241849A1 (en) * 2016-08-31 2019-08-08 University Of Kansas Expandable cell culture substrate
US20190367884A1 (en) * 2017-01-08 2019-12-05 Ramot At Tel-Aviv University Ltd. Three-dimensional tumor models, methods of manufacturing same and uses thereof
US20210403850A1 (en) * 2017-01-18 2021-12-30 National Agriculture And Food Research Organization Semipermeable membrane and uses thereof
WO2022076519A1 (en) * 2020-10-08 2022-04-14 Corning Incorporated Cell culture harvest and reseed methods and systems using dissolvable substrates

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011008986A2 (en) * 2009-07-15 2011-01-20 California Institute Of Technology A method applying hemodynamic forcing and klf2 to initiate the growth and development of cardiac valves
US20170246350A1 (en) * 2010-03-04 2017-08-31 University Of Pittsburgh - Of The Commonwealth System Of Higher Education "Bioengineered Human Corneal Stromal Tissue"
US20180243480A1 (en) * 2015-08-17 2018-08-30 The Johns Hopkins University Mesenchymal cell-binding composite material for tissue restoration
US20190241849A1 (en) * 2016-08-31 2019-08-08 University Of Kansas Expandable cell culture substrate
US20190367884A1 (en) * 2017-01-08 2019-12-05 Ramot At Tel-Aviv University Ltd. Three-dimensional tumor models, methods of manufacturing same and uses thereof
US20210403850A1 (en) * 2017-01-18 2021-12-30 National Agriculture And Food Research Organization Semipermeable membrane and uses thereof
WO2022076519A1 (en) * 2020-10-08 2022-04-14 Corning Incorporated Cell culture harvest and reseed methods and systems using dissolvable substrates

Also Published As

Publication number Publication date
WO2023217767A2 (en) 2023-11-16

Similar Documents

Publication Publication Date Title
EP4302998A3 (en) Method and system for creating pharamceutical products by additive manufacturing
KR880003610A (en) Active Ingredient Delayed-Release Formulations
EP2019133A8 (en) MICROORGANISM CAPABLE OF PRODUCING gamma-L-PGA, METHOD FOR PRODUCTION OF gamma-L-PGA USING THE MICROORGANISM, CROSSLINKED PRODUCT, AND AGENT FOR EXTERNAL APPLICATION TO THE SKIN
WO2021021350A3 (en) Artificial melanin nanoparticles and methods including porous melanin materials
WO2023217767A3 (en) Scaffold supported organoid farms for controlled high-throughput in vitro organoid aggregation and regional organoid patterning
CA2564510A1 (en) Granular material storage container and associated method
CN206927143U (en) A kind of standby ceramic tile pipeline of bridge-type
CN205209363U (en) A fiber container nozzle trimmer for on fireworks production line
WO2019216930A3 (en) Self-assembling protein scaffolds and methods
CN205570417U (en) Caking of fertilizer hog shred
CN109319667A (en) A kind of hanging apparatus for Maintenance of Agricultural Machinery
CN210552966U (en) Curved mould frock is dried by fire to acryl tunnel plate
CN205772290U (en) A kind of shawl paper subpackage arranging machine
CN207346743U (en) An egg tart skin forming machine cup device automatically
CA2314011A1 (en) Method and device for change of section of a billet of a continuous casting plant during continuous casting
CN205184738U (en) Well empty tray class part rack that wind generating set used
CN105947947A (en) Full-press plugging mechanism for semi-press plugging medicine bottle
CN207015767U (en) Drop stamping piling bed feeding pallet
CN210176140U (en) Angle modulation diaphragm connects goods shelves
CN205838514U (en) A kind of half tamponade medicine bottle total head plug mechanism
CN208047988U (en) A kind of windproof stable type seaweed drying device
CN207224218U (en) A kind of precast truss frame mould
WO2012092522A3 (en) Method, system, and apparatus for lift gas distribution
CN211590306U (en) Film-making device is used in chinese-medicinal material processing
CN216688130U (en) Monitoring device for cell culture with adjustable angle